CR20220101A - Composiciones y métodos para modificar una característica vegetal sin modificar el genoma vegetal - Google Patents

Composiciones y métodos para modificar una característica vegetal sin modificar el genoma vegetal

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Publication number
CR20220101A
CR20220101A CR20220101A CR20220101A CR20220101A CR 20220101 A CR20220101 A CR 20220101A CR 20220101 A CR20220101 A CR 20220101A CR 20220101 A CR20220101 A CR 20220101A CR 20220101 A CR20220101 A CR 20220101A
Authority
CR
Costa Rica
Prior art keywords
modifying
plant
compositions
methods
characteristic
Prior art date
Application number
CR20220101A
Other languages
English (en)
Inventor
Robert G Shatters
Michelle L Heck
Marco Pitino
Eddie W Stover
Randall P Niedz
Joseph Krystel
Magali Ferrari Grando
Original Assignee
Us Agriculture
Agrosource Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Us Agriculture, Agrosource Inc filed Critical Us Agriculture
Publication of CR20220101A publication Critical patent/CR20220101A/es

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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8291—Hormone-influenced development
    • C12N15/8295—Cytokinins
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H17/00—Symbiotic or parasitic combinations including one or more new plants, e.g. mycorrhiza
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H3/00—Processes for modifying phenotypes, e.g. symbiosis with bacteria
    • C—CHEMISTRY; METALLURGY
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    • C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C—CHEMISTRY; METALLURGY
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8291—Hormone-influenced development
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8291—Hormone-influenced development
    • C12N15/8294—Auxins
    • C—CHEMISTRY; METALLURGY
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0008—Oxidoreductases (1.) acting on the aldehyde or oxo group of donors (1.2)
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0069—Oxidoreductases (1.) acting on single donors with incorporation of molecular oxygen, i.e. oxygenases (1.13)
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1085—Transferases (2.) transferring alkyl or aryl groups other than methyl groups (2.5)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1096—Transferases (2.) transferring nitrogenous groups (2.6)
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/78—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5)
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y102/00—Oxidoreductases acting on the aldehyde or oxo group of donors (1.2)
    • C12Y102/03—Oxidoreductases acting on the aldehyde or oxo group of donors (1.2) with oxygen as acceptor (1.2.3)
    • C12Y102/03007—Indole-3-acetaldehyde oxidase (1.2.3.7)
    • C—CHEMISTRY; METALLURGY
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    • C12Y—ENZYMES
    • C12Y101/00—Oxidoreductases acting on the CH-OH group of donors (1.1)
    • C12Y101/01—Oxidoreductases acting on the CH-OH group of donors (1.1) with NAD+ or NADP+ as acceptor (1.1.1)
    • C12Y101/0111—Indolelactate dehydrogenase (1.1.1.110)
    • C—CHEMISTRY; METALLURGY
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    • C12Y—ENZYMES
    • C12Y113/00—Oxidoreductases acting on single donors with incorporation of molecular oxygen (oxygenases) (1.13)
    • C12Y113/12—Oxidoreductases acting on single donors with incorporation of molecular oxygen (oxygenases) (1.13) with incorporation of one atom of oxygen (internal monooxygenases or internal mixed function oxidases)(1.13.12)
    • C12Y113/12003—Tryptophan 2-monooxygenase (1.13.12.3)
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    • C12Y205/00—Transferases transferring alkyl or aryl groups, other than methyl groups (2.5)
    • C12Y205/01—Transferases transferring alkyl or aryl groups, other than methyl groups (2.5) transferring alkyl or aryl groups, other than methyl groups (2.5.1)
    • C12Y205/01027—Adenylate dimethylallyltransferase (2.5.1.27)
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    • C12Y206/00—Transferases transferring nitrogenous groups (2.6)
    • C12Y206/01—Transaminases (2.6.1)
    • C12Y206/01027—Tryptophan transaminase (2.6.1.27)
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    • C12Y206/00—Transferases transferring nitrogenous groups (2.6)
    • C12Y206/01—Transaminases (2.6.1)
    • C12Y206/01099—L-Tryptophan—pyruvate aminotransferase (2.6.1.99)
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    • C12Y305/00—Hydrolases acting on carbon-nitrogen bonds, other than peptide bonds (3.5)
    • C12Y305/01—Hydrolases acting on carbon-nitrogen bonds, other than peptide bonds (3.5) in linear amides (3.5.1)
    • C12Y305/01004—Amidase (3.5.1.4)
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    • C12Y—ENZYMES
    • C12Y401/00—Carbon-carbon lyases (4.1)
    • C12Y401/01—Carboxy-lyases (4.1.1)

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Environmental Sciences (AREA)
  • Developmental Biology & Embryology (AREA)
  • Botany (AREA)
  • Endocrinology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Mycology (AREA)
  • Natural Medicines & Medicinal Plants (AREA)
  • Virology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

La invención se refiere a métodos y composiciones para modificar una característica de 5 una planta sin modificar el genoma de la planta usando una o más células que comprenden uno o más genes de fitohormonas y al menos un polinucleótido de interés, que uno o más genes de fitohormonas y el al menos un polinucleótido de interés se expresan en una o más células.
CR20220101A 2019-09-20 2020-09-18 Composiciones y métodos para modificar una característica vegetal sin modificar el genoma vegetal CR20220101A (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201962903183P 2019-09-20 2019-09-20
PCT/US2020/051356 WO2021055656A1 (en) 2019-09-20 2020-09-18 Compositions and methods for modifying a plant characteristic without modifying the plant genome

Publications (1)

Publication Number Publication Date
CR20220101A true CR20220101A (es) 2022-06-28

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CR20220101A CR20220101A (es) 2019-09-20 2020-09-18 Composiciones y métodos para modificar una característica vegetal sin modificar el genoma vegetal

Country Status (13)

Country Link
US (1) US20220304273A1 (es)
EP (1) EP4031659A4 (es)
JP (2) JP2022548397A (es)
KR (1) KR20220062516A (es)
CN (1) CN114555796A (es)
AU (1) AU2020348784A1 (es)
BR (1) BR112022003563A2 (es)
CA (1) CA3154614A1 (es)
CO (1) CO2022002769A2 (es)
CR (1) CR20220101A (es)
EC (1) ECSP22018187A (es)
MX (1) MX2022002843A (es)
WO (1) WO2021055656A1 (es)

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CN112522308B (zh) * 2020-12-15 2022-04-01 山东农业大学 拟南芥tir2基因在提高植物盐胁迫抗性中的应用
CN115364019B (zh) * 2021-05-21 2023-07-04 海南师范大学 一种牛大力-石斛-诺丽复合酵素及其制备方法与作为防晒剂的应用
CN114059234B (zh) * 2021-11-26 2023-05-26 成都大学 一种果蔬保鲜膜及其制备方法和用途
CN114885842B (zh) * 2022-06-30 2023-07-28 中南林业科技大学 一种君迁子种子消毒及再生培养的方法
US20240349723A1 (en) * 2023-04-14 2024-10-24 The United States Of America, As Represented By The Secretary Of Agriculture Plant-based peptides for treatment and prevention of citrus greening disease
CN116746487A (zh) * 2023-05-30 2023-09-15 江苏多诺现代农业有限公司 一种文冠果叶片组织培养再生方法
CN118726382B (zh) * 2024-06-11 2025-01-24 中国农业大学 一种黄瓜CsaTRM4基因在促进南瓜CmoCK1砧穗间长距离运输的应用
CN118910311B (zh) * 2024-08-09 2025-08-22 湖北科技学院 桂花金属离子胁迫下lncRNA内参基因和应用

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JP4403605B2 (ja) * 1998-07-14 2010-01-27 日本製紙株式会社 再分化効率を上昇させた植物への遺伝子導入用ベクター
US20040016016A1 (en) * 2002-06-19 2004-01-22 Mankin S. Luke Compositions and methods for improving plant performance
US20060064786A1 (en) * 2004-09-17 2006-03-23 Pioneer Hi-Bred International, Inc. Isopentenyl transferase sequences and methods of use
CA2682349C (en) * 2007-04-04 2017-08-22 Basf Plant Science Gmbh Ahas mutants
JP2013201911A (ja) * 2012-03-27 2013-10-07 Hirosaki Univ 台木と穂木の接ぎ木を介して植物の形質転換を行う方法
AU2013377774A1 (en) * 2013-02-05 2015-09-17 University Of Saskatchewan Endophytic microbial symbionts in plant prenatal care
US20140380524A1 (en) * 2013-06-19 2014-12-25 The University Of Hong Kong Methods of using 3-hydroxy-3-methylglutaryl-coa synthase to enhance growth and/or seed yield of genetically modified plants
US20160333367A1 (en) * 2013-12-30 2016-11-17 Stora Enso Oyj Method for improving stem volume growth and biomass production in trees
CN108330182B (zh) * 2017-01-18 2020-04-10 中国农业大学 一种mRNA分子在植物砧穗间传递的荧光鉴定方法

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AU2020348784A1 (en) 2022-03-03
JP2022548397A (ja) 2022-11-18
ECSP22018187A (es) 2022-08-31
CN114555796A (zh) 2022-05-27
BR112022003563A2 (pt) 2022-05-24
WO2021055656A1 (en) 2021-03-25
EP4031659A1 (en) 2022-07-27
MX2022002843A (es) 2022-04-26
US20220304273A1 (en) 2022-09-29
JP2025170254A (ja) 2025-11-18
EP4031659A4 (en) 2023-09-27
KR20220062516A (ko) 2022-05-17
CO2022002769A2 (es) 2022-06-21
CA3154614A1 (en) 2021-03-25

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