CR5602A - Metodo y aparato para la determinacion por medio de un fluorescente de dos colores de molecula simple y de peso molecular y determinacionde su concentracion - Google Patents

Metodo y aparato para la determinacion por medio de un fluorescente de dos colores de molecula simple y de peso molecular y determinacionde su concentracion

Info

Publication number
CR5602A
CR5602A CR5602A CR5602A CR5602A CR 5602 A CR5602 A CR 5602A CR 5602 A CR5602 A CR 5602A CR 5602 A CR5602 A CR 5602A CR 5602 A CR5602 A CR 5602A
Authority
CR
Costa Rica
Prior art keywords
determination
probes
nucleic acid
molecula
colors
Prior art date
Application number
CR5602A
Other languages
English (en)
Inventor
Alonso Castro
John Williams
Original Assignee
Pioneer Hi Bred Int
Univ California
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Pioneer Hi Bred Int, Univ California filed Critical Pioneer Hi Bred Int
Publication of CR5602A publication Critical patent/CR5602A/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)

Abstract

un metodo de deteccion de un acido nucleico objetivo en una muestra que comprende agregar simultaneamente o secuencialmente a la muestra bajo condiciones adecuadas para hibridacion por lo menos dos sondas que lleven fluoroforo que tengan secuencias de bases nucleosidicas que sean complementarias a por lo menos una porcion de la secuencia del acido nucleico objetivo, en el que las sondas son capaces de hidridado simultaneo y especifico al acido nucleico objetivo, colocar por lo menos una porcion de la muestra que contiene sondas en un capilar que tenga una o mas zonas de deteccion, hacer que la muestra que contiene sondas se desplace a traves del capilar y detectar la emision simultanea de las sondas que llevan fluoroforo en una o mas zonas de deteccion.
CR5602A 1996-09-03 1997-08-26 Metodo y aparato para la determinacion por medio de un fluorescente de dos colores de molecula simple y de peso molecular y determinacionde su concentracion CR5602A (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US70946896A 1996-09-03 1996-09-03

Publications (1)

Publication Number Publication Date
CR5602A true CR5602A (es) 1998-08-31

Family

ID=24849984

Family Applications (1)

Application Number Title Priority Date Filing Date
CR5602A CR5602A (es) 1996-09-03 1997-08-26 Metodo y aparato para la determinacion por medio de un fluorescente de dos colores de molecula simple y de peso molecular y determinacionde su concentracion

Country Status (4)

Country Link
AR (1) AR009477A1 (es)
AU (1) AU3829197A (es)
CR (1) CR5602A (es)
WO (1) WO1998010097A2 (es)

Families Citing this family (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1998035012A2 (en) 1997-02-12 1998-08-13 Chan Eugene Y Methods and products for analyzing polymers
US6263286B1 (en) 1998-08-13 2001-07-17 U.S. Genomics, Inc. Methods of analyzing polymers using a spatial network of fluorophores and fluorescence resonance energy transfer
US6927065B2 (en) * 1999-08-13 2005-08-09 U.S. Genomics, Inc. Methods and apparatus for characterization of single polymers
US6696022B1 (en) 1999-08-13 2004-02-24 U.S. Genomics, Inc. Methods and apparatuses for stretching polymers
US6762059B2 (en) * 1999-08-13 2004-07-13 U.S. Genomics, Inc. Methods and apparatuses for characterization of single polymers
AU2002227156A1 (en) 2000-12-01 2002-06-11 Visigen Biotechnologies, Inc. Enzymatic nucleic acid synthesis: compositions and methods for altering monomer incorporation fidelity
JP2005525787A (ja) * 2001-10-24 2005-09-02 シンギュレックス・インコーポレイテッド プローブとの相互作用による遺伝子ハプロタイプの検出方法
US7371520B2 (en) 2002-05-28 2008-05-13 U.S. Genomics, Inc. Methods and apparati using single polymer analysis
US7282330B2 (en) 2002-05-28 2007-10-16 U.S. Genomics, Inc. Methods and apparati using single polymer analysis
US20040214211A1 (en) * 2003-01-23 2004-10-28 U.S. Genomics, Inc. Methods for analyzing polymer populations
US7595160B2 (en) 2004-01-13 2009-09-29 U.S. Genomics, Inc. Analyte detection using barcoded polymers
EP1704256A4 (en) 2004-01-13 2008-01-16 Us Genomics Inc Detection and quantification of analytes in solution using polymers
WO2006036388A2 (en) 2004-08-23 2006-04-06 U.S. Genomics, Inc. Systems and methods for detecting and analyzing polymers
US7262859B2 (en) 2004-10-13 2007-08-28 U.S. Genomics, Inc. Systems and methods for measurement optimization
US8045140B2 (en) * 2007-10-18 2011-10-25 Stc.Unm Method for multivariate analysis of confocal temporal image sequences for velocity estimation
US9028776B2 (en) 2012-04-18 2015-05-12 Toxic Report Llc Device for stretching a polymer in a fluid sample

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1993019205A1 (en) * 1992-03-19 1993-09-30 The Regents Of The University Of California Multiple tag labeling method for dna sequencing
EP0636186B1 (en) * 1992-04-03 1998-11-25 The Perkin-Elmer Corporation Probe composition and method
JP3068413B2 (ja) * 1994-07-13 2000-07-24 日立電子エンジニアリング株式会社 Ｄｎａ塩基配列決定装置
US6045995A (en) * 1994-08-24 2000-04-04 Isis Pharmaceuticals, Inc. Capillary electrophoretic detection of nucleic acids
DE19508366C2 (de) * 1995-03-10 1998-01-29 Evotec Biosystems Gmbh Verfahren zum direkten Nachweisen weniger Nucleinsäurestränge
DE69634696T2 (de) * 1995-10-06 2006-01-19 PerSeptive Biosystems, Inc., Framingham Verfahren und testsystem zur hybridisierungsanalyse unter verwendung von peptidnukleinsäure-sonden

Also Published As

Publication number Publication date
AR009477A1 (es) 2000-04-26
WO1998010097A2 (en) 1998-03-12
AU3829197A (en) 1998-03-26
WO1998010097A3 (en) 2002-10-17

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