CS135490A3 - Source of proteins containing other essentially biologically active compounds for nutritive purposes, particularly for feeding animals and process for preparing thereof - Google Patents

Description

The subject of the invention is a protein source containing other essential biologically active substances for nutritional purposes, in particular for feeding animals and a method of producing such a source.

Yeast biomass used in the prior art is pronounced in particular for feeding purposes and further for the preparation of biologically active substances such as vitamins, provitamins, essential amino acids, lipid saccharides and trace elements. Also production of thiobomass is known.

However, nutritional preparations from this yeast biomass do not achieve the desired and expected results, especially when achieving low nutritional efficacy. The glutin hydrolyzate is used as an additive to feed mixtures of livestock. The nutritional value is low due to the minimum content of essential amino acids. The abovementioned deficiencies removes the protein source of the process of its production according to the present invention, which consists in simply obtaining said particularly valuable source produced by culturing microbial biomass in a medium containing an alternative carbon and nitrogen source of a glutin hydrolyzate, optionally trace elements, resulting from growth-related microbiological processes microorganisms are more nutritionally biomasses with higher content and improved composition of essential amino acids, vitamins, biologically active forms of trace elements, carbohydrates and lipids.

The glutin hydrolyzate is prepared either by hydrolysis of the animal skin or by hydrolysis of the waste from various stages of the tannery production, the handling leather waste of the leather industry as part of the functionally degraded products.

According to the invention, the biomass produced is a nutritionally valuable preparation especially suited to livestock nutrition, which causes a higher incorporation of amino acids into proteins, i.e. an increase in nutrient conversion, improved health status and non-specific stimulation of the immune system. If animal skin hydrolyzate is used, there is also the possibility of humane use. When utilizing wastes from various stages of leather tanning technology, the present invention also has a great ecological importance. The accompanying examples illustrate the invention without limiting it. no. 1:

In an effective volume fermenter, a single-batch culture of Torulopsis ethanolitolorans / 26-58-1 was performed. The basic source of carbon and energy was synthetic η with 90% wt. % ethanol, the source of nitrogen was ammonia; NH3. The source of the minerals was the nutrient plant containing phosphoric acid (85%), potassium hydroxide, zinc ion, magnesium sulfate, manganese chloride and coca. An alternative source of carbon and nitrogen and a source of chromium was a glutin hydrolyzate containing 35% of dry matter with a composition of 14.9% by weight of dry matter, 8.6% of ash in dry matter and 0.078% of chromium in dry matter / aitivation was carried out at pH 4.0 and 33 ° C as follows:

To 14.5 liters of water add 20 ml of synthetic alcohol, 100 ml / l of salt, 15 g of ammonium sulphate, adjust the pH to 4.0 with sulfuric acid, add 300 g of paste (22.5% dry matter) ) the inoculum with the added carbon, nitrogen and mineral nutrient sources c cultivates until the concentration of yeast biomass reaches 60 g of dry matter / 1. From this point onwards, an alternative source of carbon, nitrogen and chromium is added depending on the consumption of the basic carbon source until the yeast biomass concentration reaches 120 g of dry matter / 1, 1.050 of the C, N, Cr source is consumed, and the yeast suspension is heated to 80 ° C and dried for 30 minutes in a spray dryer. 1,920 g of dry product of 10.6% nitrogen and 0.015% chromium were obtained.

Using an alternative source of nutrients, the bioassay yield, expressed as othanol, increased from 74.0% to 82.2% and the nutritional value increased 1.2 times compared to the biomass obtained by culturing naethanol as the sole source of carbon and energy. The favorable content of the biologically active essential element of chromium in biomass greatly increases the nutritional value of the product. Example 2 In a 2.5 l laboratory fermenter, Candida utilis biomass was continuously continually cultivated on sulfite extracts as a basic carbon source and separated on a laboratory centrifuge. 2250 ml of yeast suspension (15.0% dry matter) was obtained and aerobic culture was continued for 2 hours at 32 ° C and pH 4.2.

Upon completion of the cultivation, the suspension is heated to 80 ° C and dried in a spray dryer. A 45g product was obtained containing 69% of the crude protein from the following amino acid composition.

Composition g / 100 g of dry sample

Hydrolysate Candida Biomass Glutin product obtained before cultivation

Lysine 3.15 3.95 4.54 Histidine 1.42 1.04 1.25 Arginine 5.68 2.89 3.66 Cysteine 0.09 - 0.53 Asparagine 5.44 5.16 6.94 Threonine 0 , 63 2,61 2,78 Serine 0,73 2,62 2,27 Kglutamic 10,72 7,28 11,98 Proline 13,05 1,80 5,54 Glycine 26,15 2,08 6,75 Alanine 11.19 2.91 5.32 Valine 3.63 2.36 2.67 Methionine 1.62 0.64 1.15 Isoleucine 1.33 2.42 2.56 Leucine 3, 10 3.54 3.54 Tyrosine 4.28 1.90 3.11 Phenylalanine 1.94 2.20 2.52 Biological value 33.3 66.6 65.2 At the indicated ratio of glutin hydrolyzate and yeast biomass, i.e. 1 part glutin dry matter with biological value 33.3% of the biological value of egg white and 4.87 parts of the C.utilis biomass with a biological value of 66.6% of egg white yielded 5.8 parts of the product with a biological value of 65.2% of the biological value of egg white. 4

The biological value of the product can be controlled by ratio, for example, by an alternative and basic source of carbon and nitrogen.

Claims (1)

PATENT REQUIREMENTS 1.) Protein source containing other essential biologically active substances for nutritional purposes, especially for feeding animals. that & & It consists of microbial biomass with elevated value, essential aminociselins, vitamins, biologically active trace elements, carbohydrates and lipids. 2. The method for producing the microbial biomass according to item 17). above 65 ° C and then dried. 3.) The method of item 2, wherein the glutin hydrolyzate is prepared by hydrolyzing animal skin. 4.) Method according to items 2 and 3, that the glutin hydrolyzate is prepared by hydrolysis of wastes from various stages of the cogeneration production of leather handling wastes and leather parts of functionally deteriorated products. D 'λΖΉίνΝλΛ OHd l dvnn}' r — Ί | 0 6 .111 0 2 | aagoa 1! 6 1 9 ε 1 o I • (• 3