CS274145B1 - Inulin's insulation from vegetable juices - Google Patents
Inulin's insulation from vegetable juices Download PDFInfo
- Publication number
- CS274145B1 CS274145B1 CS598889A CS598889A CS274145B1 CS 274145 B1 CS274145 B1 CS 274145B1 CS 598889 A CS598889 A CS 598889A CS 598889 A CS598889 A CS 598889A CS 274145 B1 CS274145 B1 CS 274145B1
- Authority
- CS
- Czechoslovakia
- Prior art keywords
- inulin
- solution
- vegetable juices
- juice
- hydrogen peroxide
- Prior art date
Links
- 229920001202 Inulin Polymers 0.000 title claims abstract description 22
- JYJIGFIDKWBXDU-MNNPPOADSA-N inulin Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)OC[C@]1(OC[C@]2(OC[C@]3(OC[C@]4(OC[C@]5(OC[C@]6(OC[C@]7(OC[C@]8(OC[C@]9(OC[C@]%10(OC[C@]%11(OC[C@]%12(OC[C@]%13(OC[C@]%14(OC[C@]%15(OC[C@]%16(OC[C@]%17(OC[C@]%18(OC[C@]%19(OC[C@]%20(OC[C@]%21(OC[C@]%22(OC[C@]%23(OC[C@]%24(OC[C@]%25(OC[C@]%26(OC[C@]%27(OC[C@]%28(OC[C@]%29(OC[C@]%30(OC[C@]%31(OC[C@]%32(OC[C@]%33(OC[C@]%34(OC[C@]%35(OC[C@]%36(O[C@@H]%37[C@@H]([C@@H](O)[C@H](O)[C@@H](CO)O%37)O)[C@H]([C@H](O)[C@@H](CO)O%36)O)[C@H]([C@H](O)[C@@H](CO)O%35)O)[C@H]([C@H](O)[C@@H](CO)O%34)O)[C@H]([C@H](O)[C@@H](CO)O%33)O)[C@H]([C@H](O)[C@@H](CO)O%32)O)[C@H]([C@H](O)[C@@H](CO)O%31)O)[C@H]([C@H](O)[C@@H](CO)O%30)O)[C@H]([C@H](O)[C@@H](CO)O%29)O)[C@H]([C@H](O)[C@@H](CO)O%28)O)[C@H]([C@H](O)[C@@H](CO)O%27)O)[C@H]([C@H](O)[C@@H](CO)O%26)O)[C@H]([C@H](O)[C@@H](CO)O%25)O)[C@H]([C@H](O)[C@@H](CO)O%24)O)[C@H]([C@H](O)[C@@H](CO)O%23)O)[C@H]([C@H](O)[C@@H](CO)O%22)O)[C@H]([C@H](O)[C@@H](CO)O%21)O)[C@H]([C@H](O)[C@@H](CO)O%20)O)[C@H]([C@H](O)[C@@H](CO)O%19)O)[C@H]([C@H](O)[C@@H](CO)O%18)O)[C@H]([C@H](O)[C@@H](CO)O%17)O)[C@H]([C@H](O)[C@@H](CO)O%16)O)[C@H]([C@H](O)[C@@H](CO)O%15)O)[C@H]([C@H](O)[C@@H](CO)O%14)O)[C@H]([C@H](O)[C@@H](CO)O%13)O)[C@H]([C@H](O)[C@@H](CO)O%12)O)[C@H]([C@H](O)[C@@H](CO)O%11)O)[C@H]([C@H](O)[C@@H](CO)O%10)O)[C@H]([C@H](O)[C@@H](CO)O9)O)[C@H]([C@H](O)[C@@H](CO)O8)O)[C@H]([C@H](O)[C@@H](CO)O7)O)[C@H]([C@H](O)[C@@H](CO)O6)O)[C@H]([C@H](O)[C@@H](CO)O5)O)[C@H]([C@H](O)[C@@H](CO)O4)O)[C@H]([C@H](O)[C@@H](CO)O3)O)[C@H]([C@H](O)[C@@H](CO)O2)O)[C@@H](O)[C@H](O)[C@@H](CO)O1 JYJIGFIDKWBXDU-MNNPPOADSA-N 0.000 title claims abstract description 22
- 229940029339 inulin Drugs 0.000 title claims abstract description 22
- 235000015192 vegetable juice Nutrition 0.000 title abstract 2
- 238000009413 insulation Methods 0.000 title 1
- 238000000034 method Methods 0.000 claims abstract description 8
- 238000002955 isolation Methods 0.000 claims abstract description 4
- MHAJPDPJQMAIIY-UHFFFAOYSA-N Hydrogen peroxide Chemical compound OO MHAJPDPJQMAIIY-UHFFFAOYSA-N 0.000 claims description 16
- 235000011389 fruit/vegetable juice Nutrition 0.000 claims description 12
- 238000001914 filtration Methods 0.000 claims description 3
- 239000007788 liquid Substances 0.000 claims description 3
- 238000010908 decantation Methods 0.000 claims description 2
- 235000013311 vegetables Nutrition 0.000 claims 1
- 235000007542 Cichorium intybus Nutrition 0.000 abstract description 6
- 244000005700 microbiome Species 0.000 abstract description 3
- 239000012452 mother liquor Substances 0.000 abstract description 3
- 244000115658 Dahlia pinnata Species 0.000 abstract description 2
- 235000012040 Dahlia pinnata Nutrition 0.000 abstract description 2
- 240000008892 Helianthus tuberosus Species 0.000 abstract description 2
- 240000004808 Saccharomyces cerevisiae Species 0.000 abstract description 2
- 208000015181 infectious disease Diseases 0.000 abstract description 2
- 238000001556 precipitation Methods 0.000 abstract description 2
- 244000298479 Cichorium intybus Species 0.000 abstract 1
- 244000116484 Inula helenium Species 0.000 abstract 1
- 235000002598 Inula helenium Nutrition 0.000 abstract 1
- 241000723343 Cichorium Species 0.000 description 5
- 238000003756 stirring Methods 0.000 description 5
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Natural products CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 4
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 description 3
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 3
- 239000000126 substance Substances 0.000 description 3
- 241000196324 Embryophyta Species 0.000 description 2
- 108010090785 inulinase Proteins 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 239000000725 suspension Substances 0.000 description 2
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 1
- 235000003230 Helianthus tuberosus Nutrition 0.000 description 1
- 240000001949 Taraxacum officinale Species 0.000 description 1
- 235000005187 Taraxacum officinale ssp. officinale Nutrition 0.000 description 1
- 229910052791 calcium Inorganic materials 0.000 description 1
- 239000011575 calcium Substances 0.000 description 1
- 238000005119 centrifugation Methods 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 238000000354 decomposition reaction Methods 0.000 description 1
- 125000001495 ethyl group Chemical group [H]C([H])([H])C([H])([H])* 0.000 description 1
- 239000000706 filtrate Substances 0.000 description 1
- -1 inulin polysaccharide Chemical class 0.000 description 1
- 229940046892 lead acetate Drugs 0.000 description 1
- VTHJTEIRLNZDEV-UHFFFAOYSA-L magnesium dihydroxide Chemical compound [OH-].[OH-].[Mg+2] VTHJTEIRLNZDEV-UHFFFAOYSA-L 0.000 description 1
- 239000000347 magnesium hydroxide Substances 0.000 description 1
- 229910001862 magnesium hydroxide Inorganic materials 0.000 description 1
- 239000000395 magnesium oxide Substances 0.000 description 1
- CPLXHLVBOLITMK-UHFFFAOYSA-N magnesium oxide Inorganic materials [Mg]=O CPLXHLVBOLITMK-UHFFFAOYSA-N 0.000 description 1
- AXZKOIWUVFPNLO-UHFFFAOYSA-N magnesium;oxygen(2-) Chemical compound [O-2].[Mg+2] AXZKOIWUVFPNLO-UHFFFAOYSA-N 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 239000008267 milk Substances 0.000 description 1
- 210000004080 milk Anatomy 0.000 description 1
- 235000013336 milk Nutrition 0.000 description 1
- 229920001282 polysaccharide Polymers 0.000 description 1
- 239000005017 polysaccharide Substances 0.000 description 1
- 239000002244 precipitate Substances 0.000 description 1
- 238000003825 pressing Methods 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
- 238000003809 water extraction Methods 0.000 description 1
Landscapes
- Polysaccharides And Polysaccharide Derivatives (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
Description
Vynález se týká izolace inulínu z rostlinných štáv, například z čekanky, topinambu- u ru, černého kořene, kořene púpav, hlízy jiřiny, nebo omanu pravého novým technologickým postupem přidáním peroxidu vodíku, který kromě vysrážení inulínu chrání i matečný roztok před infekcí mikroorganizmy, například kvasinkami nebo plísněmi.The invention relates to the isolation of inulin from plant juices, for example from chicory, Jerusalem artichoke, black root, dandelion root, dahlia tuber, or omanum, by a new process by adding hydrogen peroxide which protects the mother liquor from infection with microorganisms, e.g. yeasts or molds.
Dosavadní postupy izolace inulínu ze štáv uvedených rostlin se vyznačovaly hlavně tím, že stává se nejdříve zahřála na 60 až 80 °C kvůli aktivaci rozkladného enzymu inulázy a potom se inulín vysrážel za chladu s vápenatým mlékem, dále zásaditým octanem olovnatým, popřípadě organickými srážedly, jako je alkohol ethylnatý nebo metylnatý a aceton. Vysrážený inulín se velmi těžce filtroval pro přítomnost slizovitých látek v roztoku, a matečný roztok se lehce infikoval mikroorganizmy.Previous processes for isolating inulin from the juices of said plants were characterized in that they first became heated to 60-80 ° C to activate the decomposition enzyme inulase and then the inulin precipitated in the cold with calcium milk, further with lead lead acetate or organic precipitants such as is ethyl or methyl alcohol and acetone. The precipitated inulin was filtered very heavily for the presence of mucilaginous substances in the solution, and the mother liquor was slightly infected with microorganisms.
Podle předloženého vynálezu se matečná štáva sterilizuje peroxidem vodíku, který zároveň sráží přítomné slizovité látky a inulín. Tím se technologický proces filtrace takto upraveného roztoku velmi zjednodušuje a zrychluje. Podle dosud známé technologie izolace inulínu, slizovité sloučeniny a látky bílkovinné podstaty pro svůj koloidní stav byly příčinou zalepování otvorů na filtračních plachetkách.According to the present invention, the mother juice is sterilized with hydrogen peroxide, which at the same time precipitates the mucilaginous substances and inulin present. As a result, the technological process of filtering the treated solution is greatly simplified and accelerated. According to the known technology of isolation of inulin, mucilaginous compound and protein substance due to its colloidal state, the cause of the apertures on the filter sheets was sealed.
Podstata vynálezu spočívá v tom, že do 100 hmot. dílů vylisované, nebo vyextrahované štávy s výhodou alkalicky upravené minimálně na pH 8, se za 5 až 10 minutového míchání přidá nejméně 5 hmot. dílů peroxidu vodíku v minimálně 3 procentní hmotnostní koncentraci.The principle of the invention is that up to 100 wt. At least 5 wt.% of the compressed or extracted juice, preferably alkaline adjusted to a pH of at least 8, is added with stirring for 5 to 10 minutes. % of hydrogen peroxide in a concentration of at least 3% by weight.
Z takto upraveného roztoku se za 1 až 10 hodinového stání v prostoru s teplotou 0 až -10 °C vyloučí práškový inulín, který se mechanicky oddělí od tekuté části roztoku dekantací, odstředěním, nebo filtrací a potom se vysuší.From the treated solution, standing in a space of 0-10 ° C for 1 to 10 hours, precipitated inulin powder, which was mechanically separated from the liquid part of the solution by decantation, centrifugation or filtration and then dried.
Z kořenů se inulín získává dvěma základními postupy, a to lisováním štávy z pomleté suroviny, nebo extrakcí teplou vodou. Štávu je třeba rychle ohřát na 60 až 80 °C kvůli inaktivaci enzymu inulázy a udržovat v alkalickém prostředí při pH 8 až 11. Jinak se polysacharid inulín rychle rozkládá na monomérní složky.Inulin is obtained from roots by two basic processes, either by pressing the juice from the ground material or by warm water extraction. The juice should be quickly heated to 60-80 ° C to inactivate the inulase enzyme and kept in an alkaline environment at pH 8-11. Otherwise, the inulin polysaccharide decomposes rapidly into monomeric components.
Technologicky výhodné je spojení výroby inulinu a cikorky. Z vyextrahované štávy se získává inulín vysrážením pomocí peroxidu vodíku a tekutý zbytek se dále zužitkuje na výrobu cikorky.Technologically advantageous is the combination of the production of inulin and chicory. Inulin is extracted from the extracted juice by precipitation with hydrogen peroxide and the liquid residue is further utilized for chicory production.
Příklad 1Example 1
Do dvou litrů 10 C teplé štávy vylisované ze strouhané čekanky a amoniakem zalkalizované na pH 8 se za stálého míchání přidá 0,2 litru peroxidu vodíku v 10 procentní hmotnostní koncentraci. Po 10 minutovém promíchání se obsah nádoby vloží do ledničky s teplotou -5 °C. Na druhý den se vrchní část roztoku dekantuje a usazená vrstva přáškového inulínu se promyje 1 litrem alkoholu a přefiltruje se. Filtrát se znovu promyje alkoholem, přefiltruje se a vysuší. Postup se opakuje až do požadované čistoty inulínu.To two liters of 10 C warm juice pressed from grated chicory and made alkaline to pH 8 with 0.2 l of hydrogen peroxide at 10% by weight concentration is added with stirring. After stirring for 10 minutes, the contents of the container were placed in a -5 ° C refrigerator. The next day, the top of the solution was decanted and the deposited powdered inulin layer was washed with 1 liter of alcohol and filtered. The filtrate was washed again with alcohol, filtered and dried. The procedure is repeated until the desired purity of inulin.
Příklad 2Example 2
Nastrouhané tcpinambury se vylisují. Získaná štáva v množství 2 litry se zalkalizuje přidáním oxidu hořečnatého v množství minimálně 8 gramů a přidá se 0,1 litru peroxidu vodíku ve 20 procentní hmotnostní koncentraci. Obsah takto upravené šíávy se 10 minut míchá a ochlazuje na 10 °C. Potom se vloží na 8 až 10 hodin do mrazničky a ochladí se na -5 °C. Po 8 až 10 hodinách se roztok dekantuje a zbytek roztoku, což je suzpenze inulínu se doplní jedním litrem vody, rozmíchá se a ohřeje na 60 °C po dobu 1 hodiny. Inulín přejde do roztoku, odfiltruje se a pevná část s obsahem hydroxidu hořečnatého se zachytí na filtru. Teplý roztok inulínu se ochladí.Grated tcpinambury are pressed. The juice obtained in an amount of 2 liters is made alkaline by the addition of magnesium oxide in an amount of at least 8 grams and 0.1 liters of hydrogen peroxide in a 20% by weight concentration is added. The content of the treated juice was stirred for 10 minutes and cooled to 10 ° C. It is then placed in a freezer for 8-10 hours and cooled to -5 ° C. After 8 to 10 hours, the solution is decanted and the remainder of the solution, which is a suspension of inulin, is made up with one liter of water, stirred and heated to 60 ° C for 1 hour. The inulin goes into solution, is filtered and the solid containing magnesium hydroxide is collected on the filter. The warm inulin solution is cooled.
CS 274145 BlCS 274145 Bl
Příklad 3Example 3
Do 2 litrů vylisované žíávy z nastrouhané čekanky se za míchání přidá 0,2 litru peroxidu vodíku v 10 procentní hmotnostní koncentraci. Míchá se 10 minut při teplotě 10 °C a potom se roztok vloží do ledničky s teplotou -5 °C. Po 8 hodinách se vrchní část roztoku odleje a spodní část se suspenzí inulínu se rozředí a promíchá s 1 litrem alkoholu a odfiltruje. Zachycený inulín se vakuově vysuší.To 2 liters of grated chicory juice is added 0.2 liters of hydrogen peroxide at 10% by weight with stirring. Stir for 10 minutes at 10 ° C and then place the solution in a -5 ° C refrigerator. After 8 hours, the upper part of the solution is poured and the lower part is diluted with the inulin suspension and mixed with 1 liter of alcohol and filtered. The trapped inulin is dried under vacuum.
Claims (2)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CS598889A CS274145B1 (en) | 1989-10-23 | 1989-10-23 | Inulin's insulation from vegetable juices |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CS598889A CS274145B1 (en) | 1989-10-23 | 1989-10-23 | Inulin's insulation from vegetable juices |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CS598889A1 CS598889A1 (en) | 1990-08-14 |
| CS274145B1 true CS274145B1 (en) | 1991-04-11 |
Family
ID=5405928
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CS598889A CS274145B1 (en) | 1989-10-23 | 1989-10-23 | Inulin's insulation from vegetable juices |
Country Status (1)
| Country | Link |
|---|---|
| CS (1) | CS274145B1 (en) |
-
1989
- 1989-10-23 CS CS598889A patent/CS274145B1/en unknown
Also Published As
| Publication number | Publication date |
|---|---|
| CS598889A1 (en) | 1990-08-14 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| RU2095408C1 (en) | Method of treatment of materials from yeast cells | |
| US5633032A (en) | Process of preparing cereal extracts | |
| US6972284B2 (en) | Chitosan and method of preparing chitosan | |
| US5567462A (en) | Pecto-cellulosic product from whole citrus peel and other materials | |
| CN106832041A (en) | A kind of method that biologic enzymolysis method extracts fucoidin | |
| US20100056473A1 (en) | Method of extracting fucoidan | |
| CN1231131A (en) | Insects protein capsule or powder medicine and preparation process thereof | |
| CN113249359B (en) | Method for extracting mulberry leaf functional ingredient by utilizing enzyme | |
| Stevens et al. | Structural investigation of an arabinan from cabbage (Brassica oleracea var. capitata) | |
| CN100389687C (en) | Hawthorn acetic acid fermented drink and brewage method thereof | |
| CN108485669A (en) | Seaweed activity extract and application thereof | |
| CN111171170A (en) | Method for extracting scindapsus aureus flower polysaccharide | |
| EP1397389B1 (en) | A method of isolation of immunostimulating glucan from oyster mushroom | |
| Komae et al. | Isolation of pectinesterase from Ficus awkeotsang seeds and its implication in gel-formation of the awkeotsang polygalacturonide | |
| McCready et al. | Pectin—a product of citrus waste | |
| CN116445239A (en) | Method for preparing clarified wufan wine | |
| JPS589680A (en) | Preparation of health nutrient drink containing culture fluid from fungus of genus grifola as principal constituent | |
| CN1040657C (en) | Method of extracting superoxide dismutase (SOD) from corn | |
| CN1029540C (en) | Method for preparing jelly from apple waste | |
| DE4228023A1 (en) | Immobilised mushroom starter cultures - comprising hyphae, spores and/or mycelia in polymer matrix contg. filter | |
| CN112741321A (en) | Pine pollen oral liquid and preparation method thereof | |
| SU503532A3 (en) | The method of obtaining the enzyme for lysis of microbial cells | |
| US3123538A (en) | Method for cultivating | |
| JP2742866B2 (en) | Rooibos Concentrate Extract | |
| CN110372323A (en) | A kind of preparation method of high activity composite function ceramics |