CU20120138A7 - Método de obtención de 1-kestosa - Google Patents

Método de obtención de 1-kestosa

Info

Publication number
CU20120138A7
CU20120138A7 CUP2012000138A CU20120138A CU20120138A7 CU 20120138 A7 CU20120138 A7 CU 20120138A7 CU P2012000138 A CUP2012000138 A CU P2012000138A CU 20120138 A CU20120138 A CU 20120138A CU 20120138 A7 CU20120138 A7 CU 20120138A7
Authority
CU
Cuba
Prior art keywords
kestose
sucrose
recombinant
obtaining
fos
Prior art date
Application number
CUP2012000138A
Other languages
English (en)
Other versions
CU24158B1 (es
Inventor
Cruz Enrique Rosendo Pérez
García Lázaro Hernández
García Duniesky Martínez
Toledo Luis Enrique Trujillo
Rodríguez Carmen Menéndez
Legón Alina Sobrino
Ibañez Ricardo Ramírez
Costa Gumersindo Feijoo
Rodicio Juan Manuel Lema
Original Assignee
Ct De Ingeniería Genética Y Biotecnología
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Ct De Ingeniería Genética Y Biotecnología filed Critical Ct De Ingeniería Genética Y Biotecnología
Priority to CU20120138A priority Critical patent/CU24158B1/es
Priority to ARP130103318A priority patent/AR092588A1/es
Priority to KR1020157008228A priority patent/KR102097749B1/ko
Priority to EA201590599A priority patent/EA028094B1/ru
Priority to UAA201503647A priority patent/UA118545C2/uk
Priority to MX2015003497A priority patent/MX363716B/es
Priority to EP13780057.9A priority patent/EP2899282A1/en
Priority to JP2015531460A priority patent/JP2015530089A/ja
Priority to CA2885116A priority patent/CA2885116C/en
Priority to MYPI2015700841A priority patent/MY190696A/en
Priority to IN2234DEN2015 priority patent/IN2015DN02234A/en
Priority to US14/428,534 priority patent/US10131927B2/en
Priority to BR112015005852-3A priority patent/BR112015005852B1/pt
Priority to CN201380059189.5A priority patent/CN104781414B/zh
Priority to AU2013317358A priority patent/AU2013317358B2/en
Priority to PCT/CU2013/000005 priority patent/WO2014044230A1/es
Priority to BR122021021803-0A priority patent/BR122021021803B1/pt
Publication of CU20120138A7 publication Critical patent/CU20120138A7/es
Priority to ZA2015/01833A priority patent/ZA201501833B/en
Priority to CL2015000690A priority patent/CL2015000690A1/es
Publication of CU24158B1 publication Critical patent/CU24158B1/es
Priority to AU2017221776A priority patent/AU2017221776B2/en
Priority to US16/166,215 priority patent/US10982246B2/en
Priority to JP2018203050A priority patent/JP6994449B2/ja

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/10—Organic substances
    • A23K20/163—Sugars; Polysaccharides
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
    • A23L2/00—Non-alcoholic beverages; Dry compositions or concentrates therefor; Preparation or treatment thereof
    • A23L2/52—Adding ingredients
    • A23L2/60—Sweeteners
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
    • A23L27/00—Spices; Flavouring agents or condiments; Artificial sweetening agents; Table salts; Dietetic salt substitutes; Preparation or treatment thereof
    • A23L27/30—Artificial sweetening agents
    • A23L27/33—Artificial sweetening agents containing sugars or derivatives
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
    • A23L29/00—Foods or foodstuffs containing additives; Preparation or treatment thereof
    • A23L29/30—Foods or foodstuffs containing additives; Preparation or treatment thereof containing carbohydrate syrups; containing sugars; containing sugar alcohols, e.g. xylitol; containing starch hydrolysates, e.g. dextrin
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
    • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
    • A23L5/00—Preparation or treatment of foods or foodstuffs, in general; Food or foodstuffs obtained thereby; Materials therefor
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H3/00—Compounds containing only hydrogen atoms and saccharide radicals having only carbon, hydrogen, and oxygen atoms
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H3/00—Compounds containing only hydrogen atoms and saccharide radicals having only carbon, hydrogen, and oxygen atoms
    • C07H3/02—Monosaccharides
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H3/00—Compounds containing only hydrogen atoms and saccharide radicals having only carbon, hydrogen, and oxygen atoms
    • C07H3/06—Oligosaccharides, i.e. having three to five saccharide radicals attached to each other by glycosidic linkages
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C12N9/1051—Hexosyltransferases (2.4.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/18—Preparation of compounds containing saccharide radicals produced by the action of a glycosyl transferase, e.g. alpha-, beta- or gamma-cyclodextrins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y204/00—Glycosyltransferases (2.4)
    • C12Y204/01—Hexosyltransferases (2.4.1)
    • C12Y204/01099—Sucrose:sucrose fructosyltransferase (2.4.1.99)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Polymers & Plastics (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Food Science & Technology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Nutrition Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Animal Husbandry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Mycology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Fodder In General (AREA)
  • Coloring Foods And Improving Nutritive Qualities (AREA)
  • Physiology (AREA)

Abstract

La presente invención revela un método de obtención de 1-kestosa, a escala industrial, mediante el empleo de una fructosiltransferasa (FTF) recombinante, aislada de Festuca arundinacea, expresada constitutivamente en una levadura hospedera no sacarolítica.En la invenvión, la FTF recombinante tipo sacarosa:sacarosa 1-fructosiltransferasa (1-SSTrec) se produce de forma constitutiva, estable y en altos niveles, tanto en el sobrenadante como en el sedimento celular del cultivo de una cepa de Pichia pastoris. Por tanto, también se provee un método para la obtención de 1-SST a escala industrial. La enzima recombinante resultante se emplea para la producción enzimática masiva de fructoligosacáridos (FOS), específicamente de 1-kestosa, a partir de la sacarosa.El método de la presente invención permite conversiones superiores al 55% en FOS, donde la 1-kestosa está representada en más de un 90%.
CU20120138A 2012-09-18 2012-09-18 Método de obtención de 1-kestosa CU24158B1 (es)

Priority Applications (22)

Application Number Priority Date Filing Date Title
CU20120138A CU24158B1 (es) 2012-09-18 2012-09-18 Método de obtención de 1-kestosa
ARP130103318A AR092588A1 (es) 2012-09-18 2013-09-16 Metodo de obtencion de 1-kestosa
US14/428,534 US10131927B2 (en) 2012-09-18 2013-09-18 Method for obtaining 1-kestose
CN201380059189.5A CN104781414B (zh) 2012-09-18 2013-09-18 用于获得1-蔗果三糖的方法
UAA201503647A UA118545C2 (uk) 2012-09-18 2013-09-18 Спосіб отримання 1-кестози
MX2015003497A MX363716B (es) 2012-09-18 2013-09-18 Metodo de obtencion de 1-kestosa.
EP13780057.9A EP2899282A1 (en) 2012-09-18 2013-09-18 Method for obtaining 1-kestose
JP2015531460A JP2015530089A (ja) 2012-09-18 2013-09-18 １−ケストースを得る方法
CA2885116A CA2885116C (en) 2012-09-18 2013-09-18 Method for obtaining 1-kestose
MYPI2015700841A MY190696A (en) 2012-09-18 2013-09-18 Method for obtaining 1-kestose
IN2234DEN2015 IN2015DN02234A (es) 2012-09-18 2013-09-18
KR1020157008228A KR102097749B1 (ko) 2012-09-18 2013-09-18 1-케스토스를 얻기 위한 방법
BR112015005852-3A BR112015005852B1 (pt) 2012-09-18 2013-09-18 Método para a produção de 1-sst em escala industrial
EA201590599A EA028094B1 (ru) 2012-09-18 2013-09-18 Способ получения 1-кестозы
AU2013317358A AU2013317358B2 (en) 2012-09-18 2013-09-18 Method for obtaining 1-kestose
PCT/CU2013/000005 WO2014044230A1 (es) 2012-09-18 2013-09-18 Método de obtención de 1-kestosa
BR122021021803-0A BR122021021803B1 (pt) 2012-09-18 2013-09-18 Método para a produção de 1-questose em escala industrial
ZA2015/01833A ZA201501833B (en) 2012-09-18 2015-03-17 Method for obtaining 1-kestone
CL2015000690A CL2015000690A1 (es) 2012-09-18 2015-03-18 Método de obtención de 1-kestosa a escala industrial, el cual utiliza la enzima frustosiltransferasa (ftf) recombinante aislada de festuca arundinacea; preparado enzimático; y producto alimenticio que comprende 1-kestosa.
AU2017221776A AU2017221776B2 (en) 2012-09-18 2017-08-30 Method for obtaining 1-kestose
US16/166,215 US10982246B2 (en) 2012-09-18 2018-10-22 Method for obtaining 1-kestose
JP2018203050A JP6994449B2 (ja) 2012-09-18 2018-10-29 １－ケストースを得る方法

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CU20120138A CU24158B1 (es) 2012-09-18 2012-09-18 Método de obtención de 1-kestosa

Publications (2)

Publication Number Publication Date
CU20120138A7 true CU20120138A7 (es) 2014-05-27
CU24158B1 CU24158B1 (es) 2016-03-30

Family

ID=49474178

Family Applications (1)

Application Number Title Priority Date Filing Date
CU20120138A CU24158B1 (es) 2012-09-18 2012-09-18 Método de obtención de 1-kestosa

Country Status (18)

Country Link
US (2) US10131927B2 (es)
EP (1) EP2899282A1 (es)
JP (2) JP2015530089A (es)
KR (1) KR102097749B1 (es)
CN (1) CN104781414B (es)
AR (1) AR092588A1 (es)
AU (2) AU2013317358B2 (es)
BR (2) BR122021021803B1 (es)
CA (1) CA2885116C (es)
CL (1) CL2015000690A1 (es)
CU (1) CU24158B1 (es)
EA (1) EA028094B1 (es)
IN (1) IN2015DN02234A (es)
MX (1) MX363716B (es)
MY (1) MY190696A (es)
UA (1) UA118545C2 (es)
WO (1) WO2014044230A1 (es)
ZA (1) ZA201501833B (es)

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JPWO2018117198A1 (ja) * 2017-04-14 2020-01-23 物産フードサイエンス株式会社 糖液ならびにこれを用いる液体甘味料およびハナバチ用飼料
JP2020146310A (ja) * 2019-03-14 2020-09-17 株式会社三洋物産 遊技機
JP2020146317A (ja) * 2019-03-14 2020-09-17 株式会社三洋物産 遊技機
JP2020146305A (ja) * 2019-03-14 2020-09-17 株式会社三洋物産 遊技機
JP2020146316A (ja) * 2019-03-14 2020-09-17 株式会社三洋物産 遊技機
JP2020146306A (ja) * 2019-03-14 2020-09-17 株式会社三洋物産 遊技機
JP2020146313A (ja) * 2019-03-14 2020-09-17 株式会社三洋物産 遊技機
JP2020146307A (ja) * 2019-03-14 2020-09-17 株式会社三洋物産 遊技機
JP2020146297A (ja) * 2019-03-14 2020-09-17 株式会社三洋物産 遊技機
JP2020146314A (ja) * 2019-03-14 2020-09-17 株式会社三洋物産 遊技機
JP2020146311A (ja) * 2019-03-14 2020-09-17 株式会社三洋物産 遊技機
JP2020146299A (ja) * 2019-03-14 2020-09-17 株式会社三洋物産 遊技機
JP2020146308A (ja) * 2019-03-14 2020-09-17 株式会社三洋物産 遊技機
JP2020146302A (ja) * 2019-03-14 2020-09-17 株式会社三洋物産 遊技機
JP2020146318A (ja) * 2019-03-14 2020-09-17 株式会社三洋物産 遊技機
JP2020146312A (ja) * 2019-03-14 2020-09-17 株式会社三洋物産 遊技機
JP2020146300A (ja) * 2019-03-14 2020-09-17 株式会社三洋物産 遊技機
JP2020146315A (ja) * 2019-03-14 2020-09-17 株式会社三洋物産 遊技機
JP2020146301A (ja) * 2019-03-14 2020-09-17 株式会社三洋物産 遊技機
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KR102402632B1 (ko) * 2019-10-31 2022-05-26 주식회사 삼양사 케스토스-함유 프락토올리고당 제조방법
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BR112022008663A2 (pt) * 2019-11-27 2022-07-19 Revelations Biotech Pvt Ltd Ácidos nucleicos, vetores, células hospedeiras e métodos para a produção de beta-frutofuranosidase de aspergillus niger
CN112210576B (zh) * 2020-09-28 2023-05-16 量子高科(广东)生物有限公司 一种高纯度蔗果三糖及其制备方法
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Also Published As

Publication number Publication date
JP6994449B2 (ja) 2022-02-04
AU2017221776A1 (en) 2017-09-21
US10131927B2 (en) 2018-11-20
ZA201501833B (en) 2016-08-31
AU2013317358B2 (en) 2017-06-08
EA201590599A1 (ru) 2015-07-30
CL2015000690A1 (es) 2015-08-07
MX363716B (es) 2019-03-29
US20150232898A1 (en) 2015-08-20
JP2015530089A (ja) 2015-10-15
BR122021021803B1 (pt) 2022-04-12
BR112015005852A2 (pt) 2017-07-04
CA2885116A1 (en) 2014-03-27
UA118545C2 (uk) 2019-02-11
CN104781414A (zh) 2015-07-15
US20190085364A1 (en) 2019-03-21
CA2885116C (en) 2021-09-14
AU2013317358A1 (en) 2015-04-02
CN104781414B (zh) 2019-09-03
AU2017221776B2 (en) 2019-03-21
IN2015DN02234A (es) 2015-08-21
BR112015005852B1 (pt) 2022-03-29
CU24158B1 (es) 2016-03-30
KR102097749B1 (ko) 2020-04-06
EA028094B1 (ru) 2017-10-31
MY190696A (en) 2022-05-11
MX2015003497A (es) 2015-06-22
JP2019047797A (ja) 2019-03-28
EP2899282A1 (en) 2015-07-29
AR092588A1 (es) 2015-04-29
KR20150056795A (ko) 2015-05-27
WO2014044230A1 (es) 2014-03-27
US10982246B2 (en) 2021-04-20

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