CU22287A1 - Cepa de levadura recombinante productora de quimosina bovina extracelular y procedimiento de obtencion - Google Patents

Cepa de levadura recombinante productora de quimosina bovina extracelular y procedimiento de obtencion

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Publication number
CU22287A1
CU22287A1 CU1990132A CU1990132A CU22287A1 CU 22287 A1 CU22287 A1 CU 22287A1 CU 1990132 A CU1990132 A CU 1990132A CU 1990132 A CU1990132 A CU 1990132A CU 22287 A1 CU22287 A1 CU 22287A1
Authority
CU
Cuba
Prior art keywords
security
obtaining
yeast strain
recombining
cellular
Prior art date
Application number
CU1990132A
Other languages
English (en)
Inventor
Espinosa Angela Estela Sosa
Binelfa Gerardo Ferbeyre
Adelaida Villareal
Rodriguez Alejandro Silva
Martinez Luis Saturnino Herrera
Mesa Jorge Luis Maestre
Alarcon Alredo Menendez
Madrazo Isis Del Carmen Torrens
Grillo Juan Morales
Santana Vladimir Martinez
Original Assignee
Cigb
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Cigb filed Critical Cigb
Priority to CU1990132A priority Critical patent/CU22287A1/es
Priority to DK91200074T priority patent/DK0438200T3/da
Priority to JP3014953A priority patent/JPH05184352A/ja
Priority to AT91200074T priority patent/ATE220721T1/de
Priority to EP91200074A priority patent/EP0438200B1/en
Priority to ES91200074T priority patent/ES2180528T3/es
Priority to DE69133066T priority patent/DE69133066T2/de
Publication of CU22287A1 publication Critical patent/CU22287A1/es

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  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

ESTA RELACIONADA CON LA BIOTECNOLOGIA, ESPECIALMENTE CON LA RAMA DE LA INGENIERIA GENETICA Y ESTA ENCAMINADA A LA OBTENCION DE UNA NUEVA CEPA DE LEVADURA SUPERPRODUCTORA DE QUIMOSINA POR VIA RECOMBINANTE. EL OBJETIVO TECNICO ES LOGRAR QUE A PARTIR DE UNA LEVADURA MANIPULADA GENETICAMENTE, SE OBTENGA DICHA ENZIMA EN EL MEDIO DE CULTIVO Y EN PROPORCIONES ELEVADAS POR VIA FERMENTATIVA. CON EL EMPLEO DE TECNICAS CONVENCIONALES DE INGENIERIA GENETICA, SE INTRODUJO EL GEN DE QUIMOSINA BOVINA BAJO EL PROMOTOR DE LA ALCOHOL OXIDASA 1 DE PICHIA PASTORIS Y LA SEÑAL DE SECRECION DE LA SUCROSA INVERTASA, Y SEGUIDO DE LA SECUENCIA TERMINADORA DE LA TRANSCRIPCION DE LA GLICERALDEHIDO 3 FOSFATO DESHIDROGENASA, AMBOS DE SACHAROIMYCES CEREVISIAE, CLONAJE REALIZADO EN UN VECTOR DE INTEGRACION LOGRANDOSE ASI A PARTIR DE UN MUTANTE HIS-3 DE LA LEVADURA METILOTROFA PICHIA PASTORIS UNA NUEVA CEPA CARACTERIZADA POR LA PRODUCCION DE ESTA ENZIMA PROTEOLITICA, PROPIA DEL TRACTO DIGESTIVO DE TERNEROS. LA IN VENCION ES APLICABLE EN LA OBTENCION DE UNA ENZIMA DE GRAN UTILIDAD EN LA INDUSTRIA LACTEA PARA LA ELABORACION DE QUESOS.\!
CU1990132A 1990-01-16 1990-07-12 Cepa de levadura recombinante productora de quimosina bovina extracelular y procedimiento de obtencion CU22287A1 (es)

Priority Applications (7)

Application Number Priority Date Filing Date Title
CU1990132A CU22287A1 (es) 1990-07-12 1990-07-12 Cepa de levadura recombinante productora de quimosina bovina extracelular y procedimiento de obtencion
DK91200074T DK0438200T3 (da) 1990-01-16 1991-01-16 Fremgangsmåde til ekspression af heterologe gener i gæren Pichia pastoris, ekspressionsvektorer og transformerede mikroorganismer
JP3014953A JPH05184352A (ja) 1990-01-16 1991-01-16 ピヒア パストリス（Ｐｉｃｈｉａ ｐａｓｔｏｒｉｓ）酵母中での異種遺伝子の発現方法、発現ベクターおよび形質転換微生物
AT91200074T ATE220721T1 (de) 1990-01-16 1991-01-16 Verfahren zur expression heterologischer gene in der hefe pichia pastoris, expressionsvektoren und transformierte mikroorganismen
EP91200074A EP0438200B1 (en) 1990-01-16 1991-01-16 Method for the expression of heterologous genes in the yeast Pichia pastoris, expression vectors and transformed microorganisms
ES91200074T ES2180528T3 (es) 1990-01-16 1991-01-16 Metodo de expresion de genes heterologos en la levadura pichia pastoris, vectores de expresion y microorganismos transformados.
DE69133066T DE69133066T2 (de) 1990-01-16 1991-01-16 Verfahren zur Expression heterologischer Gene in der Hefe Pichia Pastoris, Expressionsverfahren und transformierte Mikroorganismen

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CU1990132A CU22287A1 (es) 1990-07-12 1990-07-12 Cepa de levadura recombinante productora de quimosina bovina extracelular y procedimiento de obtencion

Publications (1)

Publication Number Publication Date
CU22287A1 true CU22287A1 (es) 1995-01-31

Family

ID=5459434

Family Applications (1)

Application Number Title Priority Date Filing Date
CU1990132A CU22287A1 (es) 1990-01-16 1990-07-12 Cepa de levadura recombinante productora de quimosina bovina extracelular y procedimiento de obtencion

Country Status (1)

Country Link
CU (1) CU22287A1 (es)

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