DK0438200T3 - Fremgangsmåde til ekspression af heterologe gener i gæren Pichia pastoris, ekspressionsvektorer og transformerede mikroorganismer - Google Patents

Fremgangsmåde til ekspression af heterologe gener i gæren Pichia pastoris, ekspressionsvektorer og transformerede mikroorganismer

Info

Publication number: DK0438200T3
Authority: DK; Denmark
Prior art keywords: expression; pichia pastoris; cerevisiae; gene; heterologous genes
Prior art date: 1990-01-16

Application number

DK91200074T

Other languages

English (en)

Inventor

Martinez Luis Saturnin Herrera

Gonzalez Vladimir Yong

Clark Emilio Margollez

Grillo Juan Morales

Madrazo Isis Del Carme Torrens

Binelfa Gerardo Ferbeyre

Santana Vladimir Martinez

Santiago Jorge Agustin Aguiar

Boada Julio Marcos Delgado

Rodriguez Alejandro Silva

Reyes Edenia Paifer

Osa Espinosa Angela Estela S

Menendez Alina Seralena

Lupez Tania Calle L-V Gonzalez

Segui Raquel Montesinos

Alvarez Jose Alberto Cremata

Barrios Adelaida Villareal

Badillo Beatriz Gonzalez

Alarcun Alfredo Menendez

Original Assignee

Ct Ingenieria Genetica Biotech

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1990-01-16

Filing date

1991-01-16

Publication date

2002-11-11

1990-01-16 Priority claimed from CU1990007A external-priority patent/CU22276A1/es

1990-06-13 Priority claimed from CU1990104A external-priority patent/CU22278A1/es

1990-07-12 Priority claimed from CU1990132A external-priority patent/CU22287A1/es

1990-08-15 Priority claimed from CU1990142A external-priority patent/CU22288A1/es

1991-01-16 Application filed by Ct Ingenieria Genetica Biotech filed Critical Ct Ingenieria Genetica Biotech

2002-11-11 Application granted granted Critical

2002-11-11 Publication of DK0438200T3 publication Critical patent/DK0438200T3/da

Links

108090000623 proteins and genes Proteins 0.000 title abstract 4
241000235058 Komagataella pastoris Species 0.000 title abstract 3
240000004808 Saccharomyces cerevisiae Species 0.000 title abstract 3
238000000034 method Methods 0.000 title abstract 3
239000013604 expression vector Substances 0.000 title abstract 2
244000005700 microbiome Species 0.000 title 1
235000014680 Saccharomyces cerevisiae Nutrition 0.000 abstract 2
102000006602 glyceraldehyde-3-phosphate dehydrogenase Human genes 0.000 abstract 2
108020004445 glyceraldehyde-3-phosphate dehydrogenase Proteins 0.000 abstract 2
108010025188 Alcohol oxidase Proteins 0.000 abstract 1
108090000790 Enzymes Proteins 0.000 abstract 1
102000004190 Enzymes Human genes 0.000 abstract 1
101150009006 HIS3 gene Proteins 0.000 abstract 1
108010076504 Protein Sorting Signals Proteins 0.000 abstract 1
108020004511 Recombinant DNA Proteins 0.000 abstract 1
101100394989 Rhodopseudomonas palustris (strain ATCC BAA-98 / CGA009) hisI gene Proteins 0.000 abstract 1
229930006000 Sucrose Natural products 0.000 abstract 1
CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 abstract 1
108010051210 beta-Fructofuranosidase Proteins 0.000 abstract 1
LXJXRIRHZLFYRP-UHFFFAOYSA-N glyceraldehyde 3-phosphate Chemical compound O=CC(O)COP(O)(O)=O LXJXRIRHZLFYRP-UHFFFAOYSA-N 0.000 abstract 1
230000010354 integration Effects 0.000 abstract 1
238000011031 large-scale manufacturing process Methods 0.000 abstract 1
239000003550 marker Substances 0.000 abstract 1
239000000825 pharmaceutical preparation Substances 0.000 abstract 1
229940127557 pharmaceutical product Drugs 0.000 abstract 1
229920001184 polypeptide Polymers 0.000 abstract 1
108090000765 processed proteins & peptides Proteins 0.000 abstract 1
102000004196 processed proteins & peptides Human genes 0.000 abstract 1
239000005720 sucrose Substances 0.000 abstract 1
238000013518 transcription Methods 0.000 abstract 1
230000035897 transcription Effects 0.000 abstract 1
230000009466 transformation Effects 0.000 abstract 1
239000013598 vector Substances 0.000 abstract 1

Classifications

- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
- C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
- C12N9/2405—Glucanases
- C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
- C12N9/2411—Amylases
- C12N9/2414—Alpha-amylase (3.2.1.1.)
- C12N9/2417—Alpha-amylase (3.2.1.1.) from microbiological source
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/475—Growth factors; Growth regulators
- C07K14/485—Epidermal growth factor [EGF], i.e. urogastrone
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
- C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
- C12N15/815—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts for yeasts other than Saccharomyces
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
- C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
- C12N9/6478—Aspartic endopeptidases (3.4.23)
- C12N9/6481—Pepsins (3.4.23.1; 3.4.23.2; 3.4.23.3)
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
- C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
- C12N9/6478—Aspartic endopeptidases (3.4.23)
- C12N9/6483—Chymosin (3.4.23.4), i.e. rennin
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
- C12Y304/23—Aspartic endopeptidases (3.4.23)
- C12Y304/23004—Chymosin (3.4.23.4), i.e. rennin
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
- C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence

Landscapes

Health & Medical Sciences (AREA)
Life Sciences & Earth Sciences (AREA)
Chemical & Material Sciences (AREA)
Engineering & Computer Science (AREA)
Organic Chemistry (AREA)
Genetics & Genomics (AREA)
Zoology (AREA)
Bioinformatics & Cheminformatics (AREA)
Wood Science & Technology (AREA)
Biomedical Technology (AREA)
Molecular Biology (AREA)
Biotechnology (AREA)
Biochemistry (AREA)
General Engineering & Computer Science (AREA)
General Health & Medical Sciences (AREA)
Medicinal Chemistry (AREA)
Microbiology (AREA)
Gastroenterology & Hepatology (AREA)
Biophysics (AREA)
Mycology (AREA)
Toxicology (AREA)
Proteomics, Peptides & Aminoacids (AREA)
Physics & Mathematics (AREA)
Plant Pathology (AREA)
Micro-Organisms Or Cultivation Processes Thereof (AREA)
Preparation Of Compounds By Using Micro-Organisms (AREA)
Enzymes And Modification Thereof (AREA)

DK91200074T 1990-01-16 1991-01-16 Fremgangsmåde til ekspression af heterologe gener i gæren Pichia pastoris, ekspressionsvektorer og transformerede mikroorganismer DK0438200T3 (da)

Applications Claiming Priority (4)

Application Number	Priority Date	Filing Date	Title
CU1990007A CU22276A1 (es)	1990-01-16	1990-01-16	Procedimiento para la expresion de genes heterologos en la levadura pichia pastoris, vectores de expresion y microorganismos transformados
CU1990104A CU22278A1 (es)	1990-06-13	1990-06-13	Cepa de levadura recombinante productora de una proteasa aspartica con actividad coagulante de la leche y procedimiento para su obtencion
CU1990132A CU22287A1 (es)	1990-07-12	1990-07-12	Cepa de levadura recombinante productora de quimosina bovina extracelular y procedimiento de obtencion
CU1990142A CU22288A1 (es)	1990-08-15	1990-08-15	Levadura recombinante hiperprotectora de enzima alfa amilasa termoestable y procedimiento para su obtencion

Publications (1)

Publication Number	Publication Date
DK0438200T3 true DK0438200T3 (da)	2002-11-11

Family

ID=27430148

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
DK91200074T DK0438200T3 (da)	1990-01-16	1991-01-16	Fremgangsmåde til ekspression af heterologe gener i gæren Pichia pastoris, ekspressionsvektorer og transformerede mikroorganismer

Country Status (6)

Country	Link
EP (1)	EP0438200B1 (da)
JP (1)	JPH05184352A (da)
AT (1)	ATE220721T1 (da)
DE (1)	DE69133066T2 (da)
DK (1)	DK0438200T3 (da)
ES (1)	ES2180528T3 (da)

Families Citing this family (24)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
CU22292A1 (es) *	1991-05-07	1995-01-31	Cigb	Procedimiento para la obtencion a escala industrial de licores de fructosa-glucosa a partir de sacarosa e instalacion para el mismo
DE69303943T2 (de) *	1992-02-28	1997-01-30	Suntory Ltd	Neuer Vektor, einen von Methanol und/oder Glyzerin induzierbaren Promotor enthaltend
GB9402832D0 (en) *	1994-02-15	1994-04-06	Smith Kline Beecham Biolog S A	Novel process
AU4772596A (en) *	1995-01-30	1996-08-21	New York Blood Center, Inc., The	Recombinant alpha-galactosidase enzyme
SE9501939D0 (sv) *	1995-05-24	1995-05-24	Astra Ab	DNA molecules for expression of polypeptides
US6107057A (en) *	1995-09-25	2000-08-22	Chiron Corporation	Pichia secretory leader for protein expression
CA2203613C (en) *	1997-04-24	2008-01-15	Navneet K. Ahluwalia	Secreted .alpha.-amylase as a reporter gene
FR2765589B1 (fr) *	1997-07-03	1999-09-17	Roquette Freres	Fragment d'adn comprenant le promoteur de la ribulose reductase nadph specifique de yamadazyma ohmeri, vecteur le contenant et procede de preparation de proteines utilisant ce vecteur
US6358705B1 (en)	1998-07-16	2002-03-19	Novo Nordisk A/S	Method of making proteins in transformed yeast cells
ES2231217T3 (es) *	1999-06-24	2005-05-16	Zymogenetics, Inc.	Promotor y finalizador de la gliceraldehido-3-fosfato deshidrogenasa-1 de pichia methanolica.
US6261820B1 (en)	1999-10-01	2001-07-17	Amgen Inc.	Fibronolytically active polypeptide
US6440414B1 (en)	1999-10-01	2002-08-27	Amgen Inc.	Pharmaceutical compositions of fibrinolytic agent
US7033776B2 (en)	1999-12-17	2006-04-25	Amgen Inc.	Method for treatment of indwelling catheter occlusion using fibrinolytic metalloproteinases
KR100365533B1 (ko) *	2000-05-10	2002-12-18	재단법인 목암생명공학연구소	상처치유 효과를 가진 재조합 거머린의 제조방법 및 이를 함유하는 약학적 조성물
CA2589591A1 (en) *	2004-07-22	2006-03-09	Glycofi, Inc.	Malate synthase regulatory sequences for heterologous gene expression in pichia
CU23577A1 (es)	2005-07-21	2010-09-30	Ct Ingenieria Genetica Biotech	Polipéptidos estimuladores del crecimiento para uso en peces y crustáceos
CU23636A1 (es)	2006-11-01	2011-03-21	Ct Ingenieria Genetica Biotech	Anticuerpos recombinantes contra el factor de crecimiento del endotelio vascular (vegf)
CU23634A1 (es)	2007-05-31	2011-02-24	Ct Ingenieria Genetica Biotech	Secuencias de ácido nucleico y aminoácidos, y vacuna para el control de infestaciones por ectoparásitos en peces
ES2336171B1 (es) *	2007-07-27	2011-05-11	Universidad De Santiago De Compostela	Uso de la quimosina recombinante activa.
EP2258855A1 (en)	2009-05-28	2010-12-08	Universität für Bodenkultur Wien	Expression sequences
EP2912053B1 (en)	2012-10-29	2016-11-09	Lonza Ltd	Expression sequences
CN103882050A (zh) *	2014-01-02	2014-06-25	杭州璞题生物科技有限公司	鼠表皮生长因子在巴斯德毕赤酵母中的表达方法和纯化
CN110894219A (zh) *	2019-12-26	2020-03-20	河北省微生物研究所	毕赤酵母转录因子hac1、蛋白、巴斯德毕赤酵母及其制备和应用
WO2026009000A1 (en)	2024-07-04	2026-01-08	Solasta Bio Limited	Methods for production of insecticidal neuropeptides

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
EP0148922A4 (en) *	1983-07-05	1987-06-15	Chiron Corp	SYNTHESIS USING HYBRID EPIDERMAL GROWTH FACTOR DNA.
US4721673A (en) *	1983-09-01	1988-01-26	Genex Corporation	Recovery and activation process for microbially produced calf prochymosin
US5002876A (en) *	1986-09-22	1991-03-26	Phillips Petroleum Company	Yeast production of human tumor necrosis factor
IL83435A (en) *	1986-09-23	1993-01-14	Miles Lab	Dna construct for expression of genes in yeast, an expression vector containing it and yeast transformed with said expression vector
US4997757A (en) *	1987-12-23	1991-03-05	Genebiomed, Inc.	Process for detecting potential carcinogens
JPH01240191A (ja) *	1988-02-16	1989-09-25	Green Cross Corp:The	酵母で機能する新規シグナルペプチドおよびこれを用いた異種蛋白質の分泌発現

1991
- 1991-01-16 DK DK91200074T patent/DK0438200T3/da active
- 1991-01-16 ES ES91200074T patent/ES2180528T3/es not_active Expired - Lifetime
- 1991-01-16 AT AT91200074T patent/ATE220721T1/de not_active IP Right Cessation
- 1991-01-16 EP EP91200074A patent/EP0438200B1/en not_active Expired - Lifetime
- 1991-01-16 DE DE69133066T patent/DE69133066T2/de not_active Expired - Fee Related
- 1991-01-16 JP JP3014953A patent/JPH05184352A/ja active Pending

Also Published As

Publication number	Publication date
EP0438200A1 (en)	1991-07-24
EP0438200B1 (en)	2002-07-17
JPH05184352A (ja)	1993-07-27
DE69133066T2 (de)	2003-03-06
ES2180528T3 (es)	2003-02-16
DE69133066D1 (de)	2002-08-22
ATE220721T1 (de)	2002-08-15

Publication	Publication Date	Title
ES2180528T3 (es)	2003-02-16	Metodo de expresion de genes heterologos en la levadura pichia pastoris, vectores de expresion y microorganismos transformados.
Zhong et al.	2004	Secretion, purification, and characterization of a recombinant Aspergillus oryzae tannase in Pichia pastoris
Madzak et al.	2004	Heterologous protein expression and secretion in the non-conventional yeast Yarrowia lipolytica: a review
Cregg et al.	1993	Recent advances in the expression of foreign genes in Pichia pastoris
CA2140894A1 (en)	1994-03-03	Modified fungal cells and method for producing recombinant products
EP0362179A3 (en)	1990-07-11	Recombinant saccharomyces
US4929553A (en)	1990-05-29	Protease for specific processing of secreted proteins
Kim et al.	2001	Enhanced production of anticoagulant hirudin in recombinant Saccharomyces cerevisiae by chromosomal δ-integration
Kang et al.	2005	Hansenula polymorpha
JPH0847393A (ja)	1996-02-20	酵母からタンパク質を分泌させるためのシグナル配列
KR100496758B1 (ko)	2005-06-22	한세눌라 폴리모르파 앱신 유전자 결손 변이주 및 이를이용한 재조합 단백질 생산 방법
Houard et al.	2002	Engineering of non-conventional yeasts for efficient synthesis of macromolecules: the methylotrophic genera
EP1313848B1 (en)	2006-07-05	Yeast transformant producing recombinant human parathyroid hormone and method for producing the hormone
Böer et al.	2011	Large-scale production of tannase using the yeast Arxula adeninivorans
US4940661A (en)	1990-07-10	Metallothionein transcription control sequences and use thereof
US5068185A (en)	1991-11-26	Trains of yeast for the expression of heterologous genes
US20250207143A1 (en)	2025-06-26	Recombinant yeast cell
Guengerich et al.	2004	A platform for heterologous gene expression based on the methylotrophic yeast Hansenula polymorpha
US12522838B2 (en)	2026-01-13	Promoter for yeast
KR102613937B1 (ko)	2023-12-15	갈락토스 이용에 관여하는 모든 유전자가 결손된 효모 균주 및 이를 이용한 재조합 단백질 생산방법
KR100241266B1 (ko)	2000-02-01	재조합 효모에서 인간 알파-인터페론을 고수율로 생산하는 방법
EP0994955A1 (en)	2000-04-26	Increased production of secreted proteins by recombinant yeast cells
US7091023B2 (en)	2006-08-15	Stereoselective esterase from Aspergillus oryzae
DE60128927T2 (de)	2008-02-14	Stereoselektive esterase aus aspergillus oryzae
WO2026095327A1 (ko)	2026-05-07	인공 하이브리드 프로모터