CU22683A1 - Epítopes de la proteína pre-m/m del virus del dengue, péptidos sintéticos, proteínas quiméricas y sus usos - Google Patents

Epítopes de la proteína pre-m/m del virus del dengue, péptidos sintéticos, proteínas quiméricas y sus usos

Info

Publication number
CU22683A1
CU22683A1 CU1997013A CU1997013A CU22683A1 CU 22683 A1 CU22683 A1 CU 22683A1 CU 1997013 A CU1997013 A CU 1997013A CU 1997013 A CU1997013 A CU 1997013A CU 22683 A1 CU22683 A1 CU 22683A1
Authority
CU
Cuba
Prior art keywords
protein
peptides
dengue
virus
synthetic peptides
Prior art date
Application number
CU1997013A
Other languages
English (en)
Inventor
Santiago Glay Chinea
Lazo Orlando Luis Pardo
Perez Hilda Elisa Garay
Martinez De La Cotera Jose Luis Pelegrino
Insuita Omaida Perez
Diaz Luis Morier
Vera Maylin Alvarez
Roche Rosmari Rodriguez
Antunez Maritza Pupo
Diaz Ana Beatriz Perez
Tirado Maria Guadalupe Guzman
Ramudo Susana Vazquez
Acosta Osvaldo Reyes
Nieto Gerardo Enrique Guillen
Palomares Gabriel Padron
Original Assignee
Inst De Medicina Tropical Pedro Kouri
Ct Ingenieria Genetica Biotech
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Inst De Medicina Tropical Pedro Kouri, Ct Ingenieria Genetica Biotech filed Critical Inst De Medicina Tropical Pedro Kouri
Priority to CU1997013A priority Critical patent/CU22683A1/es
Priority to PCT/CU1998/000001 priority patent/WO1998031814A1/es
Priority to AT98900843T priority patent/ATE284444T1/de
Priority to EP98900843A priority patent/EP0966535B1/en
Priority to CNB988032031A priority patent/CN1198933C/zh
Priority to US09/341,833 priority patent/US6383488B1/en
Priority to AU56499/98A priority patent/AU743414B2/en
Priority to JP53348698A priority patent/JP3647048B2/ja
Priority to ES98900843T priority patent/ES2235310T3/es
Priority to CA002277816A priority patent/CA2277816A1/en
Priority to HK00105273.7A priority patent/HK1025999B/xx
Priority to DE69828032T priority patent/DE69828032T2/de
Priority to BR9807486A priority patent/BR9807486A/pt
Priority to MYPI98000163A priority patent/MY120971A/en
Priority to NO993468A priority patent/NO993468L/no
Publication of CU22683A1 publication Critical patent/CU22683A1/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/12—Antivirals
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2770/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses positive-sense
    • C12N2770/00011—Details
    • C12N2770/24011—Flaviviridae
    • C12N2770/24111—Flavivirus, e.g. yellow fever virus, dengue, JEV
    • C12N2770/24122—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/005—Assays involving biological materials from specific organisms or of a specific nature from viruses
    • G01N2333/08—RNA viruses
    • G01N2333/18—Togaviridae; Flaviviridae
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
    • Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Virology (AREA)
  • General Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Genetics & Genomics (AREA)
  • Biophysics (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Animal Behavior & Ethology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • General Chemical & Material Sciences (AREA)
  • Oncology (AREA)
  • Communicable Diseases (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)

Abstract

La presente invención está relacionada con cinco péptidos sintéticos pertenecientes a la proteína pre-M del virus Dengue-2 (3-31, 45-67, 57-92, 69-93, 103-124), los cuales fueron denominados B 19-6, B 20-2, B 19-5, B 20-1 y B 20-3 respectivamente que cubren el 58% de su secuencia aminocídica (97/166 AA), fueron sintetizados químicamente. Dos de los péptidos (19-6 y 20-3) fueron conjugados a una proteína carrier. Los anticuerpos obtenidos tras la inmunización de ratones Balb/c con dichos conjugados fueron evaluados mediante la técnica de neutralización in vitro por reducción del número de placas. También se construyeron proteínas de fusión en las cuales se insertaron independientemente dos de las cuatro regiones cubiertas por los péptidos (1-42 y 92-133), y se expresaron en la bacteria Escherichia coli. La inmunización con cada una de estas fusiones permitirá complementar los resultados obtenidos con los péptidos sintéticos. Dos péptidos (3-31 y 103-124) resultaron capaces de inducir la producción de anticuerpos neutralizantes contra los cuatro serotipos del virus Dengue, siendo reconocidos a su vez por múltiples sueros de pacientes con diagnóstico clínico y serológico de haber sufrido una infección por el virus Dengue, mediante la técnica de Elisa indirecto. De esta manera, la presencia de epítopes secuenciales en la proteína pre-M del virus Dengue-2 queda demostrada, así como la posible relevancia de los mismos en la respuesta inmune contra dicho flavivirus.
CU1997013A 1997-01-15 1997-01-15 Epítopes de la proteína pre-m/m del virus del dengue, péptidos sintéticos, proteínas quiméricas y sus usos CU22683A1 (es)

Priority Applications (15)

Application Number Priority Date Filing Date Title
CU1997013A CU22683A1 (es) 1997-01-15 1997-01-15 Epítopes de la proteína pre-m/m del virus del dengue, péptidos sintéticos, proteínas quiméricas y sus usos
PCT/CU1998/000001 WO1998031814A1 (es) 1997-01-15 1998-01-13 Epitopes de la proteina pre-m/m del virus del dengue, peptidos sinteticos
AT98900843T ATE284444T1 (de) 1997-01-15 1998-01-13 Epitope vom pre-m/m protein aus dem dengue-virus, synthetische peptide
EP98900843A EP0966535B1 (en) 1997-01-15 1998-01-13 Epitopes of the protein pre-m/m of the dengue virus, synthetic peptides
CNB988032031A CN1198933C (zh) 1997-01-15 1998-01-13 登革病毒的前m/m蛋白的表位、合成肽
US09/341,833 US6383488B1 (en) 1997-01-15 1998-01-13 Pre-M/M epitopes of dengue virus, synthetic peptides, chimeric proteins and their use
AU56499/98A AU743414B2 (en) 1997-01-15 1998-01-13 Epitopes of the protein pre-M/M of the dengue virus, synthetic peptides
JP53348698A JP3647048B2 (ja) 1997-01-15 1998-01-13 デング熱ウイルスのプレｍ／ｍエピトープ、合成ペプチド、キメラタンパク質およびその用途
ES98900843T ES2235310T3 (es) 1997-01-15 1998-01-13 Epitopos de la proteina pre-m/m del virus del dengue, peptidos sinteticos.
CA002277816A CA2277816A1 (en) 1997-01-15 1998-01-13 Epitopes of the protein pre-m/m of the dengue virus, synthetic peptides
HK00105273.7A HK1025999B (en) 1997-01-15 1998-01-13 Epitopes of the protein pre-m/m of the dengue virus, synthetic peptides
DE69828032T DE69828032T2 (de) 1997-01-15 1998-01-13 Epitope vom pre-m/m protein aus dem dengue-virus, synthetische peptide
BR9807486A BR9807486A (pt) 1997-01-15 1998-01-13 Epìtopos pre-m/m do vìrus da dengue, peptìdios, sintéticos, proteìnas quiméricas e uso das mesmas
MYPI98000163A MY120971A (en) 1997-01-15 1998-01-15 Pre-m/m epitopes of dengue virus, synthetic peptides, chimeric proteins and its use
NO993468A NO993468L (no) 1997-01-15 1999-07-14 Syntetiske peptid-epitoper fra proteinet fra Pre-M/M fra dengue-virus

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CU1997013A CU22683A1 (es) 1997-01-15 1997-01-15 Epítopes de la proteína pre-m/m del virus del dengue, péptidos sintéticos, proteínas quiméricas y sus usos

Publications (1)

Publication Number Publication Date
CU22683A1 true CU22683A1 (es) 2001-07-20

Family

ID=5459193

Family Applications (1)

Application Number Title Priority Date Filing Date
CU1997013A CU22683A1 (es) 1997-01-15 1997-01-15 Epítopes de la proteína pre-m/m del virus del dengue, péptidos sintéticos, proteínas quiméricas y sus usos

Country Status (14)

Country Link
US (1) US6383488B1 (es)
EP (1) EP0966535B1 (es)
JP (1) JP3647048B2 (es)
CN (1) CN1198933C (es)
AT (1) ATE284444T1 (es)
AU (1) AU743414B2 (es)
BR (1) BR9807486A (es)
CA (1) CA2277816A1 (es)
CU (1) CU22683A1 (es)
DE (1) DE69828032T2 (es)
ES (1) ES2235310T3 (es)
MY (1) MY120971A (es)
NO (1) NO993468L (es)
WO (1) WO1998031814A1 (es)

Families Citing this family (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CU23245A1 (es) 2001-07-16 2007-10-17 Inst De Medicina Tropical Pedr CADENAS QUIMéRICAS CODIFICANTES PARA PROTEINAS INDUCTORAS DE EFECTOS CONTRA VIRUS. PREPARADOS UTILIZANDO PROTEINAS QUIMéRICAS
JP4500049B2 (ja) * 2001-12-04 2010-07-14 バヴァリアン・ノルディック・アクティーゼルスカブ フラビウイルスｎｓ１サブユニットワクチン
EP1454988A1 (en) * 2003-03-03 2004-09-08 Institut National De La Sante Et De La Recherche Medicale (Inserm) Infectious flavivirus pseudo-particles containing functional prM-E envelope proteins
US7281274B2 (en) * 2003-10-16 2007-10-09 Lmp Media Llc Electronic media distribution system
IN2012DN03294A (es) * 2004-07-27 2015-10-23 Government Of The Us Secretary Of The Dept Of Health And Human Services Ct S For Disease Control And
CU23578A1 (es) 2005-09-16 2010-09-30 Ct Ingenieria Genetica Biotech Proteína de la cápsida del virus dengue inductora de respuesta protectora y composición vacunal
CU23630A1 (es) 2006-10-30 2011-02-24 Ct Ingenieria Genetica Biotech Moléculas peptídicas quiméricas con propiedades antivirales contra los virus de la familia flaviviridae
WO2009024534A2 (en) * 2007-08-17 2009-02-26 Intercell Ag Japanese encephalitis virus (jev) and tick-borne encephalitis virus (tbev) peptides stimulating human t cell responses
CU20080028A6 (es) * 2008-02-29 2011-02-24 Ct Ingenieria Genetica Biotech Compuestos químicos obtenidos in silico para la preparación de composiciones farmacéuticas para atenuar o inhibir la infección por virus dengue y otros flavivirus
NZ592054A (en) * 2008-10-13 2013-06-28 Inst Research In Biomedicine Dengue virus neutralizing antibodies and uses thereof
CN101921310B (zh) * 2009-06-15 2014-03-12 温州医学院 登革病毒特异性hla-a2限制性表位肽及应用
WO2011133112A1 (en) * 2010-04-20 2011-10-27 National University Of Singapore Cell penetrating peptide derived from the premembrane protein of flavivirus
BR112013030637A2 (pt) * 2011-05-26 2018-06-12 Inst De Medicina Molecular peptídeo derivado do vírus da dengue (denv) e métodos para a inibição da replicação de flavivírus
CU24188B1 (es) * 2012-12-27 2016-07-29 Ct De Ingeniería Genética Y Biotecnología Composición vacunal contra el virus dengue
CA2918644A1 (en) * 2013-06-26 2014-12-31 The University Of North Carolina At Chapel Hill Methods and compositions for dengue virus vaccines
TWI769185B (zh) 2016-10-27 2022-07-01 海樂源有限公司 登革熱類病毒顆粒、抗登革熱病毒抗體、及含其之組合物
CN116082499B (zh) * 2021-11-08 2023-10-27 东莞市朋志生物科技有限公司 一种抗登革ns1蛋白的抗体及其应用

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU6093296A (en) * 1995-06-07 1996-12-30 Government Of The United States Of America, As Represented By The Secretary Of The Department Of Health And Human Services, The Infectious dengue 2 virus pdk-53 as quadravalent vaccine

Also Published As

Publication number Publication date
CA2277816A1 (en) 1998-07-23
JP2001508457A (ja) 2001-06-26
HK1025999A1 (en) 2000-12-01
DE69828032D1 (de) 2005-01-13
WO1998031814A1 (es) 1998-07-23
NO993468L (no) 1999-09-14
EP0966535A1 (en) 1999-12-29
NO993468D0 (no) 1999-07-14
CN1198933C (zh) 2005-04-27
US6383488B1 (en) 2002-05-07
ES2235310T3 (es) 2005-07-01
AU5649998A (en) 1998-08-07
AU743414B2 (en) 2002-01-24
EP0966535B1 (en) 2004-12-08
DE69828032T2 (de) 2005-12-01
JP3647048B2 (ja) 2005-05-11
BR9807486A (pt) 2000-03-21
CN1249781A (zh) 2000-04-05
ATE284444T1 (de) 2004-12-15
MY120971A (en) 2005-12-30

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