CU22813A1 - OLIGONUCLEOTIDES TO DETECT BACTERIES OF THE FAMILY OF THE BETA-LACTAMIC ANTIBIOTICS PRODUCING FAMILIES AND DETECTION KIT OF THE SAME - Google Patents
OLIGONUCLEOTIDES TO DETECT BACTERIES OF THE FAMILY OF THE BETA-LACTAMIC ANTIBIOTICS PRODUCING FAMILIES AND DETECTION KIT OF THE SAMEInfo
- Publication number
- CU22813A1 CU22813A1 CU1998182A CU1998182A CU22813A1 CU 22813 A1 CU22813 A1 CU 22813A1 CU 1998182 A CU1998182 A CU 1998182A CU 1998182 A CU1998182 A CU 1998182A CU 22813 A1 CU22813 A1 CU 22813A1
- Authority
- CU
- Cuba
- Prior art keywords
- beta
- family
- oligonucleotides
- bacteries
- detect
- Prior art date
Links
- 241000894006 Bacteria Species 0.000 title abstract 2
- 108091034117 Oligonucleotide Proteins 0.000 title abstract 2
- JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 title abstract 2
- 239000003242 anti bacterial agent Substances 0.000 title abstract 2
- 229940088710 antibiotic agent Drugs 0.000 title abstract 2
- 238000001514 detection method Methods 0.000 title 1
- 241000186361 Actinobacteria <class> Species 0.000 abstract 2
- 101150074325 pcbC gene Proteins 0.000 abstract 2
- 238000003752 polymerase chain reaction Methods 0.000 abstract 2
- 238000009007 Diagnostic Kit Methods 0.000 abstract 1
- 230000003321 amplification Effects 0.000 abstract 1
- 238000004458 analytical method Methods 0.000 abstract 1
- 150000001875 compounds Chemical class 0.000 abstract 1
- 238000000034 method Methods 0.000 abstract 1
- 238000003199 nucleic acid amplification method Methods 0.000 abstract 1
- 150000003952 β-lactams Chemical class 0.000 abstract 1
Landscapes
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
Se desarrolló un método y además un kit ó juego diagnóstico para identificar bacterias del grupo de los Actinomicetos productoras de compuestos de la familia de los (Beta-lactámicos ó sus derivados. Se disenaron oligonuclétidos sintéticos (SEQ ID NO 1 y SEQ ID NO 2) a partir del análisis de varias secuencias del gen pcbC de Actinomicetos productores de estos antibióticos los cuales fueron usados como cebadores en un Sistema de Reacción en Cadena de la Polimerasa (PCR) de 35 ciclos para la amplificación de una región de aproximadamente 700-750 bp del gen pcbC.A method and a diagnostic kit or kit were also developed to identify bacteria from the group of Actinomycetes that produce compounds of the family of (Beta-lactams or their derivatives. Synthetic oligonucleotides (SEQ ID NO 1 and SEQ ID NO 2) were designed) from the analysis of several sequences of the pcbC gene of Actinomycetes producing these antibiotics which were used as primers in a 35-cycle Polymerase Chain Reaction System (PCR) for the amplification of a region of approximately 700-750 bp of the pcbC gene.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CU1998182A CU22813A1 (en) | 1998-12-01 | 1998-12-01 | OLIGONUCLEOTIDES TO DETECT BACTERIES OF THE FAMILY OF THE BETA-LACTAMIC ANTIBIOTICS PRODUCING FAMILIES AND DETECTION KIT OF THE SAME |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CU1998182A CU22813A1 (en) | 1998-12-01 | 1998-12-01 | OLIGONUCLEOTIDES TO DETECT BACTERIES OF THE FAMILY OF THE BETA-LACTAMIC ANTIBIOTICS PRODUCING FAMILIES AND DETECTION KIT OF THE SAME |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CU22813A1 true CU22813A1 (en) | 2003-01-29 |
Family
ID=46599416
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CU1998182A CU22813A1 (en) | 1998-12-01 | 1998-12-01 | OLIGONUCLEOTIDES TO DETECT BACTERIES OF THE FAMILY OF THE BETA-LACTAMIC ANTIBIOTICS PRODUCING FAMILIES AND DETECTION KIT OF THE SAME |
Country Status (1)
| Country | Link |
|---|---|
| CU (1) | CU22813A1 (en) |
-
1998
- 1998-12-01 CU CU1998182A patent/CU22813A1/en unknown
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US8329394B2 (en) | Methods and substances for isolation and detection of small polynucleotides | |
| AU2012251027B2 (en) | Quantitative nuclease protection Assay (qNPA) and sequencing (qNPS) improvements | |
| US20040023209A1 (en) | Method for identifying microorganisms based on sequencing gene fragments | |
| ATE232242T1 (en) | UNIVERSAL PRIMER SEQUENCE FOR MULTIPLEX DNA AMPLIFICATION | |
| CN102242189A (en) | Nucleic Acid Template preparation for real-time PCR | |
| CN114085896A (en) | Multiplex pyrosequencing using non-interfering noise-canceling polynucleotides identification tags | |
| CN106574304B (en) | Strand Invasion-Based DNA Amplification Methods | |
| JP2012080871A (en) | Method for directly detecting rna | |
| DE60143314D1 (en) | VOTING THE PRESENCE OF CRYPTOSPORIDIUM ORGANISMS IN A TEST TEST | |
| CN105358711A (en) | Strand-invasion based DNA amplification method | |
| EP3209675A1 (en) | Compositions and methods for the detection of nucleic acids | |
| US20220228150A1 (en) | Crispr system high throughput diagnostic systems and methods | |
| ES2685710T3 (en) | Kits comprising controls for nucleic acid amplification | |
| ES2151002T3 (en) | DETECTION OF TREPONEMA PALLIDUM AND HAEMOPHILUS DUCREYI. | |
| WO2019073049A1 (en) | Solid phase isothermal amplification | |
| CA3046266A1 (en) | Nucleic acid catenane with a linking duplex biosensor for detection of a microorganism target | |
| Chhabra et al. | Molecular detection methods of foodborne viruses | |
| AR027256A1 (en) | DETECTION OF MICROORGANISMS RELATED TO FERMENTATION | |
| US20130130258A1 (en) | Detection, identification and differentiation of eubacterial taxa using a hybridization assay | |
| EP2414541B1 (en) | Methylation ligation-dependent macroarray (mlm) | |
| ES2253279T3 (en) | METHODS AND COMPOSITIONS FOR THE DETECTION OF SPECIES OF THE MYCOBACTERIUM AVIUM COMPLEX. | |
| ATE347618T1 (en) | METHOD FOR AMPLIFICATION OF NUCLEIC ACIDS PROVIDING STRAND SEPARATION MEANS AND UNIVERSAL SEQUENCE MARKERS | |
| RU2420589C1 (en) | SYNTHETIC OLIGONUCLEOTIDES KIT FOR DNA DETECTION OF PARODONTOPATHOGENIC MICROORGANISM Prevotella intermedia BY POLYMERASE CHAIN REACTION | |
| WO2006035062A1 (en) | Detection, identification and differentiation of serratia species using the spacer region | |
| CN113512609A (en) | Novel coronavirus real-time fluorescent nucleic acid isothermal amplification detection kit and its special primers and probes |