CU22813A1 - Oligonucleótidos para detectar bacterías de la familia de los actinomicetos productoras de antibióticos beta-lactámicos y kit de detección de los mismos - Google Patents
Oligonucleótidos para detectar bacterías de la familia de los actinomicetos productoras de antibióticos beta-lactámicos y kit de detección de los mismosInfo
- Publication number
- CU22813A1 CU22813A1 CU1998182A CU1998182A CU22813A1 CU 22813 A1 CU22813 A1 CU 22813A1 CU 1998182 A CU1998182 A CU 1998182A CU 1998182 A CU1998182 A CU 1998182A CU 22813 A1 CU22813 A1 CU 22813A1
- Authority
- CU
- Cuba
- Prior art keywords
- beta
- family
- oligonucleotides
- bacteries
- detect
- Prior art date
Links
- 241000894006 Bacteria Species 0.000 title abstract 2
- 108091034117 Oligonucleotide Proteins 0.000 title abstract 2
- JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 title abstract 2
- 239000003242 anti bacterial agent Substances 0.000 title abstract 2
- 229940088710 antibiotic agent Drugs 0.000 title abstract 2
- 238000001514 detection method Methods 0.000 title 1
- 241000186361 Actinobacteria <class> Species 0.000 abstract 2
- 101150074325 pcbC gene Proteins 0.000 abstract 2
- 238000003752 polymerase chain reaction Methods 0.000 abstract 2
- 238000009007 Diagnostic Kit Methods 0.000 abstract 1
- 230000003321 amplification Effects 0.000 abstract 1
- 238000004458 analytical method Methods 0.000 abstract 1
- 150000001875 compounds Chemical class 0.000 abstract 1
- 238000000034 method Methods 0.000 abstract 1
- 238000003199 nucleic acid amplification method Methods 0.000 abstract 1
- 150000003952 β-lactams Chemical class 0.000 abstract 1
Landscapes
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
Se desarrolló un método y además un kit ó juego diagnóstico para identificar bacterias del grupo de los Actinomicetos productoras de compuestos de la familia de los (Beta-lactámicos ó sus derivados. Se disenaron oligonuclétidos sintéticos (SEQ ID NO 1 y SEQ ID NO 2) a partir del análisis de varias secuencias del gen pcbC de Actinomicetos productores de estos antibióticos los cuales fueron usados como cebadores en un Sistema de Reacción en Cadena de la Polimerasa (PCR) de 35 ciclos para la amplificación de una región de aproximadamente 700-750 bp del gen pcbC.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CU1998182A CU22813A1 (es) | 1998-12-01 | 1998-12-01 | Oligonucleótidos para detectar bacterías de la familia de los actinomicetos productoras de antibióticos beta-lactámicos y kit de detección de los mismos |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CU1998182A CU22813A1 (es) | 1998-12-01 | 1998-12-01 | Oligonucleótidos para detectar bacterías de la familia de los actinomicetos productoras de antibióticos beta-lactámicos y kit de detección de los mismos |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CU22813A1 true CU22813A1 (es) | 2003-01-29 |
Family
ID=46599416
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CU1998182A CU22813A1 (es) | 1998-12-01 | 1998-12-01 | Oligonucleótidos para detectar bacterías de la familia de los actinomicetos productoras de antibióticos beta-lactámicos y kit de detección de los mismos |
Country Status (1)
| Country | Link |
|---|---|
| CU (1) | CU22813A1 (es) |
-
1998
- 1998-12-01 CU CU1998182A patent/CU22813A1/es unknown
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