DD228974A3 - Verfahren zur herstellung von carnitindehydrogenase - Google Patents
Verfahren zur herstellung von carnitindehydrogenase Download PDFInfo
- Publication number
- DD228974A3 DD228974A3 DD24909083A DD24909083A DD228974A3 DD 228974 A3 DD228974 A3 DD 228974A3 DD 24909083 A DD24909083 A DD 24909083A DD 24909083 A DD24909083 A DD 24909083A DD 228974 A3 DD228974 A3 DD 228974A3
- Authority
- DD
- German Democratic Republic
- Prior art keywords
- carnitine
- enzyme
- dehydrogenase
- activity
- carnitine dehydrogenase
- Prior art date
Links
- 108010029922 carnitine dehydrogenase Proteins 0.000 title claims abstract description 23
- 238000004519 manufacturing process Methods 0.000 title claims abstract 4
- 230000000694 effects Effects 0.000 claims abstract description 23
- 238000000034 method Methods 0.000 claims abstract description 18
- 108090000790 Enzymes Proteins 0.000 claims abstract description 16
- 102000004190 Enzymes Human genes 0.000 claims abstract description 16
- 241000589776 Pseudomonas putida Species 0.000 claims abstract description 7
- 239000000284 extract Substances 0.000 claims abstract description 6
- 238000000746 purification Methods 0.000 claims abstract description 3
- 238000002360 preparation method Methods 0.000 claims abstract 4
- 235000019750 Crude protein Nutrition 0.000 claims abstract 3
- 238000005516 engineering process Methods 0.000 claims abstract 2
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 claims description 11
- 229910052921 ammonium sulfate Inorganic materials 0.000 claims description 9
- 235000011130 ammonium sulphate Nutrition 0.000 claims description 9
- 102000004169 proteins and genes Human genes 0.000 claims description 8
- 108090000623 proteins and genes Proteins 0.000 claims description 8
- 238000005119 centrifugation Methods 0.000 claims description 6
- VLKZOEOYAKHREP-UHFFFAOYSA-N n-Hexane Chemical compound CCCCCC VLKZOEOYAKHREP-UHFFFAOYSA-N 0.000 claims description 6
- 230000008014 freezing Effects 0.000 claims description 5
- 238000007710 freezing Methods 0.000 claims description 5
- 241000894006 Bacteria Species 0.000 claims description 4
- 230000029087 digestion Effects 0.000 claims description 4
- 238000010257 thawing Methods 0.000 claims description 4
- 229920002684 Sepharose Polymers 0.000 claims description 3
- 238000000605 extraction Methods 0.000 claims description 3
- 239000013049 sediment Substances 0.000 claims description 3
- 230000003247 decreasing effect Effects 0.000 claims description 2
- 238000011081 inoculation Methods 0.000 claims description 2
- 239000000725 suspension Substances 0.000 claims description 2
- PHIQHXFUZVPYII-ZCFIWIBFSA-N (R)-carnitine Chemical compound C[N+](C)(C)C[C@H](O)CC([O-])=O PHIQHXFUZVPYII-ZCFIWIBFSA-N 0.000 claims 6
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims 4
- 229910052760 oxygen Inorganic materials 0.000 claims 4
- 239000001301 oxygen Substances 0.000 claims 4
- 108010066477 Carnitine O-acetyltransferase Proteins 0.000 claims 2
- 102100036357 Carnitine O-acetyltransferase Human genes 0.000 claims 2
- 241000589516 Pseudomonas Species 0.000 claims 2
- 229960004203 carnitine Drugs 0.000 claims 2
- DIOQZVSQGTUSAI-UHFFFAOYSA-N decane Chemical compound CCCCCCCCCC DIOQZVSQGTUSAI-UHFFFAOYSA-N 0.000 claims 2
- 239000007788 liquid Substances 0.000 claims 2
- 230000003287 optical effect Effects 0.000 claims 2
- KIUMMUBSPKGMOY-UHFFFAOYSA-N 3,3'-Dithiobis(6-nitrobenzoic acid) Chemical compound C1=C([N+]([O-])=O)C(C(=O)O)=CC(SSC=2C=C(C(=CC=2)[N+]([O-])=O)C(O)=O)=C1 KIUMMUBSPKGMOY-UHFFFAOYSA-N 0.000 claims 1
- 102100026024 Acyl-coenzyme A synthetase ACSM3, mitochondrial Human genes 0.000 claims 1
- 229920001817 Agar Polymers 0.000 claims 1
- 102000005870 Coenzyme A Ligases Human genes 0.000 claims 1
- 102000003855 L-lactate dehydrogenase Human genes 0.000 claims 1
- 108700023483 L-lactate dehydrogenases Proteins 0.000 claims 1
- 108010011449 Long-chain-fatty-acid-CoA ligase Proteins 0.000 claims 1
- 102000013460 Malate Dehydrogenase Human genes 0.000 claims 1
- 108010026217 Malate Dehydrogenase Proteins 0.000 claims 1
- 238000009825 accumulation Methods 0.000 claims 1
- 238000005273 aeration Methods 0.000 claims 1
- 239000008272 agar Substances 0.000 claims 1
- 239000012491 analyte Substances 0.000 claims 1
- 238000004458 analytical method Methods 0.000 claims 1
- 238000004587 chromatography analysis Methods 0.000 claims 1
- 239000000287 crude extract Substances 0.000 claims 1
- 238000003745 diagnosis Methods 0.000 claims 1
- 230000002255 enzymatic effect Effects 0.000 claims 1
- 230000002209 hydrophobic effect Effects 0.000 claims 1
- 239000002054 inoculum Substances 0.000 claims 1
- 230000002452 interceptive effect Effects 0.000 claims 1
- 229940049920 malate Drugs 0.000 claims 1
- BJEPYKJPYRNKOW-UHFFFAOYSA-N malic acid Chemical compound OC(=O)C(O)CC(O)=O BJEPYKJPYRNKOW-UHFFFAOYSA-N 0.000 claims 1
- 244000005700 microbiome Species 0.000 claims 1
- 235000015097 nutrients Nutrition 0.000 claims 1
- 230000035790 physiological processes and functions Effects 0.000 claims 1
- 238000001556 precipitation Methods 0.000 claims 1
- 238000011084 recovery Methods 0.000 claims 1
- 150000003839 salts Chemical class 0.000 claims 1
- 239000007787 solid Substances 0.000 claims 1
- 239000000126 substance Substances 0.000 claims 1
- 238000002560 therapeutic procedure Methods 0.000 claims 1
- 238000009210 therapy by ultrasound Methods 0.000 claims 1
- 238000009423 ventilation Methods 0.000 claims 1
- 230000000813 microbial effect Effects 0.000 abstract 1
- 239000006228 supernatant Substances 0.000 description 4
- PHIQHXFUZVPYII-UHFFFAOYSA-N carnitine Chemical compound C[N+](C)(C)CC(O)CC([O-])=O PHIQHXFUZVPYII-UHFFFAOYSA-N 0.000 description 2
- 239000008363 phosphate buffer Substances 0.000 description 2
- 229910016876 Fe(NH4)2(SO4)2 Inorganic materials 0.000 description 1
- 230000003698 anagen phase Effects 0.000 description 1
- 239000000470 constituent Substances 0.000 description 1
- 238000010828 elution Methods 0.000 description 1
- 238000000855 fermentation Methods 0.000 description 1
- 230000004151 fermentation Effects 0.000 description 1
- 238000011049 filling Methods 0.000 description 1
- 239000002244 precipitate Substances 0.000 description 1
- 238000004537 pulping Methods 0.000 description 1
- 238000000527 sonication Methods 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
Landscapes
- Enzymes And Modification Thereof (AREA)
- Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
Priority Applications (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DD24909083A DD228974A3 (de) | 1983-03-23 | 1983-03-23 | Verfahren zur herstellung von carnitindehydrogenase |
| BG6423684A BG49226A1 (en) | 1983-03-23 | 1984-02-13 | Method for preparing carnitindehydrogenase |
| CS225284A CS254131B1 (en) | 1983-03-23 | 1984-03-28 | Method of carnitine dehydrogenase production |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DD24909083A DD228974A3 (de) | 1983-03-23 | 1983-03-23 | Verfahren zur herstellung von carnitindehydrogenase |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| DD228974A3 true DD228974A3 (de) | 1985-10-23 |
Family
ID=5545844
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DD24909083A DD228974A3 (de) | 1983-03-23 | 1983-03-23 | Verfahren zur herstellung von carnitindehydrogenase |
Country Status (3)
| Country | Link |
|---|---|
| BG (1) | BG49226A1 (cs) |
| CS (1) | CS254131B1 (cs) |
| DD (1) | DD228974A3 (cs) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5156966A (en) * | 1989-10-13 | 1992-10-20 | Toyo Jozo Kabushiki Kaisha | L-carnitine dehydrogenase and process for its production |
-
1983
- 1983-03-23 DD DD24909083A patent/DD228974A3/de not_active IP Right Cessation
-
1984
- 1984-02-13 BG BG6423684A patent/BG49226A1/xx unknown
- 1984-03-28 CS CS225284A patent/CS254131B1/cs unknown
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5156966A (en) * | 1989-10-13 | 1992-10-20 | Toyo Jozo Kabushiki Kaisha | L-carnitine dehydrogenase and process for its production |
Also Published As
| Publication number | Publication date |
|---|---|
| BG49226A1 (en) | 1991-09-16 |
| CS254131B1 (en) | 1988-01-15 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| ENJ | Ceased due to non-payment of renewal fee |