DD240215A5 - Neue monoklonale antikoerper gegen glycokonjugate, verfahren zu ihrer herstellung und ihre anwendung - Google Patents

Neue monoklonale antikoerper gegen glycokonjugate, verfahren zu ihrer herstellung und ihre anwendung Download PDF

Info

Publication number: DD240215A5
Authority: DD; German Democratic Republic
Prior art keywords: monoclonal antibodies; cells; item; derivatives; gsl
Prior art date: 1984-08-30

Application number

DD28012085A

Other languages

German (de)

English (en)

Inventor

Guenter Rosenfelder

Harry Towbin

Dietmar Braun

Original Assignee

Ciba-Geigy Ag,Ch

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1984-08-30

Filing date

1985-08-29

Publication date

1986-10-22

1985-08-29 Application filed by Ciba-Geigy Ag,Ch filed Critical Ciba-Geigy Ag,Ch

1986-10-22 Publication of DD240215A5 publication Critical patent/DD240215A5/de

Links

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Micro-Organisms Or Cultivation Processes Thereof (AREA)
Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
Peptides Or Proteins (AREA)

DD28012085A 1984-08-30 1985-08-29 Neue monoklonale antikoerper gegen glycokonjugate, verfahren zu ihrer herstellung und ihre anwendung DD240215A5 (de)

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- 1985-06-19 GB GB858515538A patent/GB8515538D0/en active Pending
- 1985-08-29 JP JP60188688A patent/JPS6193200A/ja active Pending
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Publication	Publication Date	Title
DE3887675T2 (de)	1994-06-30	Antikörper.
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DE3883803T2 (de)	1994-04-14	Monoklonale Antikörper gegen melanoma-assoziierte Antigene, Hybridzellinien, die diese Antikörper produzieren, und ihre Verwendung.
DE69432926T2 (de)	2004-05-13	Humanes carcinoma-antigen (hca), hca antikörper, hca immunoassays, aufzeichnungsmethoden und therapy
EP0260610B1 (de)	1993-09-01	Monoklonale Antikörper gegen humanen Tumonekrosefaktor (TNF) und deren Verwendung
EP0173648A2 (en)	1986-03-05	New monoclonal antibodies to glycoconjugates, processes for their production, and applications
DE69130224T2 (de)	1999-05-06	Monoklonaler antikörper
DE69306803T2 (de)	1997-05-15	Kleinzelliger lungenkarzinom-spezifischer antikörper und antigenen
EP0480440B1 (de)	1996-05-01	Monoklonale Antikörper gegen Melanom
DE69033295T2 (de)	2000-05-25	Nachweismethode für Harnkarzinom-assoziierte Antigene
AT400577B (de)	1996-01-25	Verfahren zur herstellung eines monoklonalen antikörpers und verfahren zum nachweisen von malignen zellen
DE3346336A1 (de)	1984-08-23	Verfahren zur herstellung von erythropoetin
DE69911322T2 (de)	2004-07-01	Monoklonaler antikörper der die oligosaccharide sialinsäure n-glycolierte galactose-glucose auf bösartige tumoren erkennt und zusammensetzungen die dieser enthalten
DE3789781T2 (de)	1994-11-10	Antigene, Antikörper und Verfahren zur Identifizierung humaner metastatischer Tumoren und Zellinien zur Herstellung dieser Antikörper.
DE3586216T2 (de)	1992-12-10	Brusttumorassoziiertes antigen und monoklonale antikoerper dazu.
DE68922806T2 (de)	1996-02-29	Monoklonale antikörper und herstellung eines impfstoffes gegen menschliche krebsantigene durch immunisierung mit menschlichem und tierischem mucin und mit synthetischen gluciden-trägerkonjugaten.
DE3587976T2 (de)	1995-05-18	Monoklonale antikörper und antigene für menschliche lungenkarzinome vom typ der nichtkleinen zellen.
DE3884646T2 (de)	1994-01-27	Humanes Karcinom-assoziertes Antigen und an dieses Antigen bindende Antikörper.
DE3688638T2 (de)	1993-12-09	Monoklonale Antikörper für humane Lungenkarzinome des "Nicht-kleine-Zellen"-Typs.
DE3785795T2 (de)	1993-09-02	Monoklonale anti-menschliche magenkrebs-antikoerper.
DE69031225T2 (de)	1997-12-11	Monoklonaler antikörper zur differenzierung von "squamous cell carcinoma"-antigenen und verfahren zu dessen verwendung
DE69124683T2 (de)	1997-09-11	Monoklonale Antikörper gegen Glykolipid-Karbohydratketten
DE69024758T2 (de)	1996-08-29	Neuartige antiidiotypische monoklonale Antikörper
DE69114546T2 (de)	1996-04-04	Neue Verwendung für einen monoklonalen Antikörper.
DE68913601T2 (de)	1994-10-13	Monoklonaler Antikörper gegen menschliches Lungenadenokarzinom.