DD260280A5 - Verfahren zur renaturierung von denaturierten proteinen - Google Patents
Verfahren zur renaturierung von denaturierten proteinen Download PDFInfo
- Publication number
- DD260280A5 DD260280A5 DD87301550A DD30155087A DD260280A5 DD 260280 A5 DD260280 A5 DD 260280A5 DD 87301550 A DD87301550 A DD 87301550A DD 30155087 A DD30155087 A DD 30155087A DD 260280 A5 DD260280 A5 DD 260280A5
- Authority
- DD
- German Democratic Republic
- Prior art keywords
- protein
- concentration
- renaturation
- reactivation
- solution
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/96—Stabilising an enzyme by forming an adduct or a composition; Forming enzyme conjugates
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
- C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
- C12N9/2462—Lysozyme (3.2.1.17)
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/107—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides
- C07K1/113—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides without change of the primary structure
- C07K1/1136—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides without change of the primary structure by reversible modification of the secondary, tertiary or quarternary structure, e.g. using denaturating or stabilising agents
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
- C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
- C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
- C12N9/6424—Serine endopeptidases (3.4.21)
- C12N9/6456—Plasminogen activators
- C12N9/6459—Plasminogen activators t-plasminogen activator (3.4.21.68), i.e. tPA
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
- C12Y304/21—Serine endopeptidases (3.4.21)
- C12Y304/21069—Protein C activated (3.4.21.69)
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- General Engineering & Computer Science (AREA)
- Medicinal Chemistry (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Crystallography & Structural Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biophysics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Peptides Or Proteins (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Enzymes And Modification Thereof (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DE19863611817 DE3611817A1 (de) | 1986-04-08 | 1986-04-08 | Verfahren zur renaturierung von proteinen |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| DD260280A5 true DD260280A5 (de) | 1988-09-21 |
Family
ID=6298240
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DD87301550A DD260280A5 (de) | 1986-04-08 | 1987-04-06 | Verfahren zur renaturierung von denaturierten proteinen |
Country Status (20)
| Country | Link |
|---|---|
| US (1) | US4933434A (fr) |
| EP (1) | EP0241022B1 (fr) |
| JP (1) | JPH0742307B2 (fr) |
| KR (1) | KR900005279B1 (fr) |
| AT (1) | ATE84541T1 (fr) |
| AU (1) | AU583723B2 (fr) |
| CA (1) | CA1304191C (fr) |
| CZ (1) | CZ283304B6 (fr) |
| DD (1) | DD260280A5 (fr) |
| DE (2) | DE3611817A1 (fr) |
| DK (1) | DK173650B1 (fr) |
| ES (1) | ES2038969T3 (fr) |
| FI (1) | FI93842C (fr) |
| GR (1) | GR3007605T3 (fr) |
| HK (1) | HK46896A (fr) |
| HU (1) | HU198514B (fr) |
| IE (1) | IE60064B1 (fr) |
| IL (1) | IL82091A (fr) |
| SK (1) | SK249587A3 (fr) |
| ZA (1) | ZA872474B (fr) |
Families Citing this family (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1989001484A1 (fr) * | 1987-08-14 | 1989-02-23 | Gebro Broschek Kg Pharmazeutische Fabrik | Procede d'oxydation intramoleculaire de deux groupes -sh dans un compose peptidique de façon a former un pont bisulfure et peptide produit selon ce procede |
| DE3832898A1 (de) * | 1988-09-28 | 1990-04-12 | Boehringer Mannheim Gmbh | Praeparat von in prokaryonten exprimiertem plasminogenaktivator |
| DE3835350A1 (de) * | 1988-10-17 | 1990-04-19 | Boehringer Mannheim Gmbh | Aktivierung von gentechnologisch hergestellten, in prokaryonten exprimierten antikoerpern |
| DE3903581A1 (de) * | 1989-02-07 | 1990-08-16 | Boehringer Mannheim Gmbh | Gewebs-plasminogenaktivator-derivat |
| EP0545999B1 (fr) * | 1990-08-20 | 1997-06-04 | Novo Nordisk A/S | Procédé de préparation d l'IGF-1 biologiquement actif à partir d'un IGF-1 ayant une extension amino-terminale |
| ES2119779T3 (es) * | 1990-09-05 | 1998-10-16 | Southern Cross Biotech Pty Ltd | Solubilizacion de proteinas en formas activas. |
| US5023323A (en) * | 1990-09-12 | 1991-06-11 | Monsanto Company | Method of somatotropin naturation |
| DE4037196A1 (de) * | 1990-11-22 | 1992-05-27 | Boehringer Mannheim Gmbh | Verfahren zur reaktivierung von denaturiertem protein |
| DE4139000A1 (de) | 1991-11-27 | 1993-06-03 | Boehringer Mannheim Gmbh | Verfahren zur gentechnologischen herstellung von biologisch aktivem ss-ngf |
| EP1077263A1 (fr) | 1999-07-29 | 2001-02-21 | F.Hoffmann-La Roche Ag | Procédé de préparation des protéines secrétées sous une forme pliée naturelle par co-sécrétion de chaperones |
| US20050079526A1 (en) * | 2002-02-20 | 2005-04-14 | Affinium Pharmaceuticals, Inc. | Methods and apparatuses for characterizing refolding and aggregation of biological molecules |
| WO2011113601A1 (fr) | 2010-03-17 | 2011-09-22 | Biogenerix Ag | Procédé permettant d'obtenir un g-csf humain recombinant biologiquement actif |
| WO2012104099A1 (fr) | 2011-02-04 | 2012-08-09 | Glucometrix Ag | Procédé de production de trypsine recombinante |
| CN106699836A (zh) * | 2017-03-07 | 2017-05-24 | 安徽鑫华坤生物工程有限公司 | 一种蛋白质复性的透析设备 |
| CN120341163B (zh) * | 2025-06-20 | 2025-09-30 | 深圳市中芯辰瑞光电科技有限公司 | 一种固晶头 |
Family Cites Families (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4216141A (en) | 1978-07-19 | 1980-08-05 | The Salk Institute For Biological Studies | Method for cyclization of peptides |
| DE3231658A1 (fr) | 1981-01-21 | 1983-11-17 | ||
| WO1983004418A1 (fr) * | 1982-06-07 | 1983-12-22 | Celltech Limited | Procede de preparation de chymosine |
| JPS593711U (ja) * | 1982-06-30 | 1984-01-11 | 協伸工業株式会社 | 電線被覆剥取器 |
| AU538170B2 (en) * | 1982-09-15 | 1984-08-02 | Owens-Illinois Glass Container Inc. | Modified protein to mould an enzyme type molecule |
| GR79124B (fr) * | 1982-12-22 | 1984-10-02 | Genentech Inc | |
| ATE107356T1 (de) * | 1983-03-25 | 1994-07-15 | Celltech Ltd | Verfahren zur herstellung eines proteins. |
| US4530787A (en) * | 1984-03-28 | 1985-07-23 | Cetus Corporation | Controlled oxidation of microbially produced cysteine-containing proteins |
| KR860007688A (ko) | 1985-03-08 | 1986-10-15 | 시끼 모리야 | 전자석장치(電磁石裝置) |
| GB8508340D0 (en) * | 1985-03-29 | 1985-05-09 | Creighton T E | Production of protein |
| EP0208539A3 (fr) * | 1985-07-11 | 1988-08-03 | Repligen Corporation | Protéines pliantes liées par pont disulfuré en croix |
| US4656255A (en) * | 1985-09-09 | 1987-04-07 | International Minerals & Chemical Corp. | Protein recovery |
| DE3618817A1 (de) * | 1986-06-04 | 1987-12-10 | Behringwerke Ag | Verfahren zur gewinnung aktiver proteine aus einer biologisch inaktiven form |
-
1986
- 1986-04-08 DE DE19863611817 patent/DE3611817A1/de not_active Withdrawn
-
1987
- 1987-03-16 IE IE69587A patent/IE60064B1/en not_active IP Right Cessation
- 1987-04-02 IL IL82091A patent/IL82091A/xx not_active IP Right Cessation
- 1987-04-02 CA CA000533687A patent/CA1304191C/fr not_active Expired - Lifetime
- 1987-04-06 DD DD87301550A patent/DD260280A5/de not_active IP Right Cessation
- 1987-04-06 JP JP62083132A patent/JPH0742307B2/ja not_active Expired - Lifetime
- 1987-04-07 HU HU871493A patent/HU198514B/hu unknown
- 1987-04-07 CZ CS872495A patent/CZ283304B6/cs not_active IP Right Cessation
- 1987-04-07 KR KR1019870003281A patent/KR900005279B1/ko not_active Expired
- 1987-04-07 FI FI871522A patent/FI93842C/fi not_active IP Right Cessation
- 1987-04-07 SK SK2495-87A patent/SK249587A3/sk unknown
- 1987-04-07 ZA ZA872474A patent/ZA872474B/xx unknown
- 1987-04-08 EP EP87105230A patent/EP0241022B1/fr not_active Expired - Lifetime
- 1987-04-08 DE DE8787105230T patent/DE3783497D1/de not_active Expired - Lifetime
- 1987-04-08 DK DK198701797A patent/DK173650B1/da active IP Right Grant
- 1987-04-08 AU AU71190/87A patent/AU583723B2/en not_active Ceased
- 1987-04-08 ES ES198787105230T patent/ES2038969T3/es not_active Expired - Lifetime
- 1987-04-08 AT AT87105230T patent/ATE84541T1/de not_active IP Right Cessation
-
1989
- 1989-01-13 US US07/298,274 patent/US4933434A/en not_active Expired - Lifetime
-
1993
- 1993-04-09 GR GR920403237T patent/GR3007605T3/el unknown
-
1996
- 1996-03-14 HK HK46896A patent/HK46896A/xx not_active IP Right Cessation
Also Published As
| Publication number | Publication date |
|---|---|
| HUT44046A (en) | 1988-01-28 |
| IL82091A (en) | 1991-12-15 |
| CA1304191C (fr) | 1992-06-23 |
| HK46896A (en) | 1996-03-22 |
| GR3007605T3 (fr) | 1993-08-31 |
| SK279976B6 (sk) | 1999-06-11 |
| HU198514B (en) | 1989-10-30 |
| FI871522L (fi) | 1987-10-09 |
| DE3783497D1 (de) | 1993-02-25 |
| JPS62242697A (ja) | 1987-10-23 |
| US4933434A (en) | 1990-06-12 |
| IE870695L (en) | 1987-10-08 |
| IE60064B1 (en) | 1994-06-01 |
| ATE84541T1 (de) | 1993-01-15 |
| FI93842B (fi) | 1995-02-28 |
| EP0241022A3 (en) | 1989-10-25 |
| DE3611817A1 (de) | 1987-10-15 |
| AU583723B2 (en) | 1989-05-04 |
| DK179787D0 (da) | 1987-04-08 |
| ES2038969T3 (es) | 1993-08-16 |
| CZ249587A3 (en) | 1997-11-12 |
| IL82091A0 (en) | 1987-10-30 |
| JPH0742307B2 (ja) | 1995-05-10 |
| FI871522A0 (fi) | 1987-04-07 |
| SK249587A3 (en) | 1999-06-11 |
| KR900005279B1 (ko) | 1990-07-27 |
| EP0241022A2 (fr) | 1987-10-14 |
| AU7119087A (en) | 1987-10-15 |
| CZ283304B6 (cs) | 1998-02-18 |
| DK179787A (da) | 1987-10-09 |
| DK173650B1 (da) | 2001-05-21 |
| EP0241022B1 (fr) | 1993-01-13 |
| ZA872474B (en) | 1988-07-27 |
| FI93842C (fi) | 1995-06-12 |
| KR870010185A (ko) | 1987-11-30 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| RPI | Change in the person, name or address of the patentee (searches according to art. 11 and 12 extension act) | ||
| ENJ | Ceased due to non-payment of renewal fee |