DD283545A5 - METHOD FOR STERILIZING THE DUST BAGS OF PLANTS - Google Patents

Abstract

The invention relates to a process for the sterilization of the anthers of plants, which makes it possible to sterilize anthers of a wide range of cultures with a high degree of sterilization while maintaining the ability of seeds, fruit preparations with free Bestäubung. The invention has for its object to find sterilizing agent for the process for the sterilization of dust bags. According to the invention, the treatment of the plants with a sterilizing agent containing a dismutating agent is carried out in the fifth and / or sixth period of the organogenesis. As active substances in the sterilizing agent, N-substituted maleamic acids according to the invention are of the general formula RNHCOCHCHCO 2 H, in which {anther plants; Sterilization procedures; Sterilant; Active ingredient N-substituted maleamic acids; Agriculture; Selection; Seed Production}

Description

Designation of the invention VSRi ¹ AHRSN FOR THE STERILIZATION OF DUST BAGS FROM PLANTS field of use

The present invention relates to biology and agriculture, more particularly to a method of sterilizing the dust bags of plants used in selection and seed building.

Characteristic you ochu ^ kt ^ S / & ccj cUr Tec ^ n / d:

Today, in the world, the task of intensifying agriculture, in particular to increase the carrying capacity of cereals, feed, vegetable and technical crops by broad use of first-generation hybrids, is being solved. Because of their hybrid power, the hybrids differ from the molds in higher performance (by 25 to 30%) and better product quality.

Ea there is a process for the production of new hybrids, which is based on a system "cytoplasinate pollen sterility - Restorer of fertility". This procedure is an ongoing (within 12 to 14 years)

ο \

, , - 2 - ZiSStS

and complicated selection work involving the development of sterile analogs, sterility fixators and restorers of fertility. Particularly promising are methods based on the sterilization of the anthers of plants with chemical sterilants (gametocides). The use of gametocides is significantly more cost effective than the systemic cytoplasmic penile sterility ¹¹ , since there is no need to produce such forms of sterile analogue, analogues for the fixation of sterility in maternal forms and for the restoration of fertility in paternal forms In practice, it is possible to obtain the seeds of first-generation hybrids both in the selection of initial forms and in the organization of their technical production.

At present, about 200 compounds are found which possess gametocidal activity and their chemical structure belongs to various classes of chemical compounds. Gametocides must provide full pollen minerality to treated plants while maintaining the viability of bracts and to ensure a sufficiently high ability (desirably at least 70% of the control) to form the ovary under free pollination. The levels of their phytotoxicity and toxicity to warm-blooded animals must be minimal ,

Methods are known for sterilizing the dust bags of cereal crops (L.Dzh .. Nikell, Regulatory Rosta rasteny, Primenenie ν selakom khozyaistve, Moskva, izdatelstvo "Kolos", 1984, p 2Ö-51, SU, A 906457 · LJNikell., Growth regulators of Plants · Application in agriculture Moscow,

Verlag "Koloa", 1984, p 2Ö-31), which in the treatment of plants with Stariliaationaniittein never 2-Chloräthylphoaphonsaure (fithrel), maleic acid hydrazide, di (polyfluoroalkyl) - phosphoric acids and their salts u.a. consist. The treatment of the plants with domestic sterilizing agents takes place in the V and VX. Period of organogenesis (after F.M. Kupermann).

In the 5th period of an organogenesis processes of formation and differentiation of flowers begin. Towards this period neoplasms, sporogenous archesporewebs develop. During this period stamens, pistils and perianth are applied. In the fifth period the differentiation of the hump of stamens into stamens and pistils appears. The VI. Period is characterized by the process of flowering (micro- and macrosporogenesis). In this period, separate mononuclear pollen grains are formed (FM Koupermann, Morfofiziologiya rest. * Moskva, izdatelstvo "Vysshaya shkola", 1973, pp. 30-36.) MorphoPhysiology of Plants., Moscow, Publisher "Vysschaya shkola", 1973. p. 36).

Also known is a method of sterilizing the anthers of grassy plants (GB, A, 1567153), which consists of treating the grass plants with a sterilizing agent in the period between the occurrence of the second stem member and earspacing. As a sterilizing agent one uses he ο cyclic compounds whose main representatives are 2-karbo.xy-3,4-methanpyrrolidine or 2-methoxycarbonyl-3,4-methanpyrrolidin · The compounds mentioned are used in combination with diluents and surfactants.

Object of the invention

The aim of the present invention is to develop a method of sterilizing anthers which makes it possible to use it for the sterilization of anthers of a wide range of cultures with a high degree of sterilization while maintaining the high ability of seeds to produce fruiting with free pollination ,

Explanation of the essence of the invention

The object of the invention is to select sterilizing agents in the process for the sterilization of anthers.

In accordance with the stated object and the stated object, the invention is that in the proposed method for sterilizing the anthers of plants by their treatment with a sterilant in conjunction with a diluent in the fifth and / or sixth period of the organogenesis according to the invention as a sterilant Η -substituted maleamic acids of the general formula

BHHCOCH _s CHCO ₂ H,

wherein R (CH ₃ ) ₂ NCH ₂ CH ₂ -; (CHj) ₂ NCH ₂ CH ₂ CH ₂ -; C ₃ H ₅ -; HOC ₂ H ₄ -; NH ₂ CO - means «

The sterilant can be used in combination with any known and suitable diluent. It is expedient to use Ss in conjunction with water as a 0.1 to 2% aqueous emulsion. As plants, which are treated with the indicated sterilizing agent, are preferably used grass plants or sunflowers.

The method according to the invention makes it possible to

To achieve sterility of plants (98-100%) and to obtain a high percentage of ability to seed (over 70%). In order to achieve the high degree of sterilization of dust bags under unfavorable climatic conditions, the treatment of plants with the sterilizing agent is repeated in the fifth and / or sixth perlode of the organogen (according to Kupermann).

The process according to the invention is carried out as follows.

Plants such as; Winter and summer wheat, diploid and tetraploid rye, triticale, millet, sunflower, grass plants are treated with a sterilizing agent, using as such N-substituted-thymiceaminic acids.

N-substituted maleamic acids can be used in conjunction with any suitable thinner, as a thinner water is suitably used. In this case, a 0.1 to 2% aqueous emulsion of the compounds given to be used advantageously.

It is possible to add any suitable surface-active substances to the ready-to-use solutions as desired. When applied to plants, it is convenient to add to the ready-to-use solutions any known adjunct additives such as wetting agents, dispersants and adhesives.

The sterilizing agent can be applied to plants by various treatment methods such as liquid atomization and air atomization (aerosols). The treatment

of plants with the sterilizing agent takes place in the fifth and / or sixth period of organogenesis (according to Kupermann) · The dose of the sterilizing agent depends on the nature of the compound! of culture to be treated «treatment period and natural climatic factors. In order to ensure the high sterilization efficiency under unfavorable climatic conditions, it is expedient to use a less complicated treatment of plants in the VI. Period of organogenesis. The total dose of sterilant is 0.6 to 20 kg / ha.

All of the N-substituted maleamic acids of the invention which are used as sterilants have been tested for toxicity in animal studies. The Prüfergebni as β have shown that the indicated compounds of low or medium toxicity. LDc ₀ for N- (2-dimethylamethyl) maleamic acid is 2500 mg / kg animal weight, for N- (3-dimethylaminopropyl) maleamic acid over 10000 mg / kg, for N-ethylmaleamic acid 2000 mg / kg, for N- (2-hydroxyethyl ) maleamic acid 1500 mg / kg and N-kaxbamoylmaleamic acid 2000 mg / kg.

The gametocidal activity of the sterilizing agent according to the invention was demonstrated in field trials in various soil climatic zones with sections of 10 m in size three to four times repetition. Each sterilant was tested for at least 5 years.

The development of the periods of organogenesis is overwhelmed cytologically. The treatment of plants with the Sterilisat ionamittel occurs at the beginning of the fifth period of organogenesis after Kapermann.

By ear pushing you realize the insulation

-7- of main clocks and other floors, in parchment. For wheat and insecticides, single isolators are used. In the case of rye, 5 to 7 different plants next to each other are placed under a common insulator per ear of wheat * In the case of millet, each panicle is isolated separately. The sterility percentage (X) for wheat, bog, triticale and millet is given by the following formula

, Number of inseminated in an isolator

X =

I -

Seeds of treated plants

X 100 %

Number of seeds fertilized in an isolator of ui-treated control plants.

The number of grains in uninsulated ears of control plants is assumed to be 100 % on free pollination.

To obtain reliable data, 20 to 25 isolators of each replicate are used for wheat and triticae, 10 to 15 group isolators of each replicate for rye and millet.

To control the chemical sterilization of sunflower pollen, 45 treated patches of each replicate are used for each compound, 15 plants being isolated from them for self-pollination of the flower basket, and 15 other plants pollinated with 20 to 25 treated isolated pots and 15 plants should be left for free pollination so that the preparation of achenes can be controlled with the pollen of the father form.

Pollen sterility of plants of the Son

-a-

according to the morphological characteristics of spermatozoa and the ability of achenes to prepare for pollination of the treated isolated plants with the pollen of the untreated paternal form. The viability of the egg cell is determined after seed formation of treated plants with free pollination of the paternal form.

It is desirable to carry out the treatment of plants in clear windless weather.

N-substituted mal earn ins are known compounds. They are prepared according to the known technology, for example by reacting the maleic anhydride and the corresponding amine.

embodiments

For a better understanding of the present invention, the following specific embodiments are given.

example 1

Plants of winter wheat variety Mironovskaya 608 are treated in the fifth period of organogenesis by spraying a 2% solution of aqueous Sauls of the N- (2-dimethylethylamino) -maleamic acid with the aid of a syringe.

As the emulsifying agent, 0.1 mass% of calcium alkylbenzenesulfonate having 12 to 14 carbon atoms is added. The adjuvant is introduced into the solution 0.01% by mass of dimethyl sulphoxide. The consumption of preparation is 20 kg / ha. As control serve plants, treated with a thinner without sterilizing agent. The results are shown in Table 1. , Examples 2 to 5

The procedure is analogous to that described in Example 1.

The sterilant used is a 2% aqueous emulsion of N- (3-dimethylaminopropyl) -maleamic acid, N-ethylmaleamic acid, N- (2-hydroxyethyl) maleaminae, N-carbamoylmaleamic acid. The test results are shown in Table 1.

The similar results are in the treatment of wheat variety Mironovskaya 808 in the VI. Period of organogenesis obtained ·

Table 1

Ser , at 1 number of percentage 0.0 number of percent No. game 2 grains the sterili 99.8 grains sentence of 3 the ear of wheat ty 99.8 the ear of wheat Anset- 4 in the iso 99.5 at free zens of 5 lator 99.3 pollination grains 100.0 at free he be pollination 1. control 42.1 42.6 100.0 Second example 0.1 38.3 89.9 Third example 0.1 41-5 97.4 4th example 0.2 40.7 95.5 5th example 0.3 39.8 93.4 6th example 0.0 40.0 93.9

Examples up to 10

Plants of winter wheat variety Bezostaya I are obtained in the V. period of organogenesis by sputtering a 2% aqueous emulsion of N- (2-dimethylaminoethyl) maleic acid onto the acid, N- (2-dimethylaminopropyl) -malamic acid, N-ethylmaleamic acid, N - (2-hydroxyethyl) -maleamic acid, N-Karbamoylmaleaminsäure treated by means of a syringe. The JSmulsion contains 0.1% by mass of calcium alkylbenzenesulfonate having 12 to 14 carbon atoms.

The following are introduced into the emulsion O ₁ oil mass% tetrahydrofuran as adjuvant.

The consumption of preparation is 16 kg / ha. As control serve plants, treated with a thinner without sterilization agent ·

The Prüfergehnisse are shown in Table 2. Similar results are received at treatment of winter wheat of a Bezostaya type I in VI · period of an organogenesis.

Table 2

Ser , at 6 number of 31.7 percentage number of Prozentsai No. game 7 grains 0.0 the steri Körhern of the Anset a the ear of wheat 0.0 formality the ear of wheat zens of 9 in Isola 0.2 at free grains 10 gate 0.0 he be at free 0.7 pollination he be pollination 1. control 0.0 42.9 100.0 Second example 100.0 39.7 92.5 Third example 100.0 40.2 93.7 4th example 99.4 42.1 9ö, l 5th example 100.0 37.1 36.5 6th example 97, a 4i, a 97.4

Examples up to 15

Plants of spring wheat variety Moekovskaya 35 warden -maleaminsäure in V. period of organogenesis (after Kuper man) with a ljSigen aqueous emulsion of N- (2-dimethylaminoethyl), N- (3-dimethyl minopropyl _a) -maleaminsäure, N-Äthylmaleaminsäure, N- (2-hydroxyethyl) maleamic acid <3 or N-karbaaioylmal a treated on the acid. The Sauls ion contains 0.5 mass% KaIziumalkylbenzolsulfonat having 12 to 14 carbon atoms

zn s if s

- ii -

as a bulking agent and 0.01% by weight of dimethylformamide as Adouvazxa * The consumption of preparation is 10 kg / ha. As a control serve plants treated with a thinner without Sterilisationamittel.

The test results are shown in Table 3. The similar results are in the treatment of winter wheat of the Moskovskaya variety 35 In VI. Period of organogenesis obtained.

Table 3

Ser , at 11 number percentage number percentage No. game 12 from Kör the steri from Kör of attachment 13 the formality the of grains 14 Ear of corn in the - Ear of wheat at free 15 insulator free pollination Bestäu advertising 1. control 27.1 0.0 27.5 100.0 Second example 0.2 99.3 26.8 97.4 Third example 0.3 98.9 27.0 98.2 4th example 0.4 98.5 26.7 97.1 5th example 0.0 100.0 27.0 98.2 6th example 0.0 100.0 27.2 98.9

Examples 16 to 20

Plants of summer wheat variety Botanicheskaya 4 are in the V. period of organogenesis (according to Kupermann) with a 1% aqueous emulsion of N- (2-dimethylaminoethyl) -maleamic acid, N- (5-dimethylaminopropyl) -maleamic acid, N-ethylmaleamic acid, N- (2-hydroxyethyl) -maleamic acid or N-Karbamoylmaleaminsäure treated. The emulsion contains 0.5% by weight of calcium alkylbenzenesulfonate having 12 to 14 carbon atoms and 0.01% by mass of dimethyl sulfoxide as Ad, j uv ans. The consumption of

Preparation is 6 kg / ha · ale control serve plants treated with a thinner without sterilizing agent.

The test results are shown in Table 4. Anal results are at treatment of summer wheat variety Botanicheskaya 4 in VI. Period of the organ. received nogenesis.

Table 4

Ser example ä 16 number percentage number percentage pollination 100.0 No. 17 from Kör the sterili from Kör of piecing 30.1 99.0 ia the ty the of grains 29.8 92.0 19 ' her im Ear of wheat at free 27.7 93.7 20 insulator free pollination 2a, 2 95.3 2A, 7 90.0 1. Kontrollt 2Q, 2 0.0 27.1 Second example 0.7 97.5 Third example 0.1 99.7 4th example 0.0 100.0 5th example 0.0 100.0 6th example 0.0 100.0

Examples 21 to 25

Plants of tetraploid Hoggen aorte Belta are in the V.Periode of Organogeneae (Kapler) with a 1% aqueous emulsion of N- (2-ßimethylaminoäthyl) maleamic, N- (3-dimethylaiainopropyl) maleamic acid, N-Äthylmaleaminsäure, N- (2 ^ tiydroxyäthyl) maleamic acid or Kaxbamoylmaleaminsäure treated. The emulsion contains 0.5 parts by weight of calcium alkylbenzenesulfonate having 12 to 14 carbon atoms and 0.01% by weight of tetrahydrofuran. The consumption of preparation is 10 kg / ha. The test results are shown in Table 5. The similar results are in the treatment of tetraploid rye variety Belta in the VI. Period of organi-

obtained genesis. Table 5

Ser. example .Number percentage number percent No. of body the sterili from Kör sentence of "Nern the ty the piecing Ear of corn in the Ear of wheat from Kör insulator free loading ners pollination free loading pollination

1. Control 21 50.6 0.0 2nd example 22 0.0 100.0 3 * example 23 0.0 100.0 4th example 24 0.3 99.4 5, example 25 0.2 99.6 6th example 0.0 .100,0

54.8 48.2 47.7 52.3 51.6 47, β

100.0 88.2 87.0 95.4 94.2 87.2

Examples 26 to 28

Plants of tetraploid Eoggen sort Ukrainskaya tetra ηerden in the V. Period of Organogeneae (after Küpermann) with a 1% aqueous emulsion of N- (2-dimethylaminoethyl) maleamic acid, N- (3-dimethylaminopropyl) maleamic acid and N-Karbamoylmale β treated. The version contains 0.10% by mass of calcium alkyl benzene sulphonate having from 12 to 14 carbon atoms and 0.01% by weight of dodecyl sulphate. The consumption of preparation is 6 kg / ha. As control serve plants, treated with a thinner without sterilizing agent. The test results are shown in Table 6. Similar results are at treatment of tetraploid rye Ukrainskaya in VI. Period of organogenesis preserved »

Table 6

Running example Number Percentage No. Percentage No. of body Ster11i of bodies Ansatz

ners of the zens of Kör-Ähre in the ear at ners

Insulator of free free

Pollination exercise

1. Control 32.6 0.0 44.8 - 100.0

2. Example 26 0.0 100.0 40.1 89.5

3. Example 27 0.0 100.0 42.6 95.1

4. Example 28 0.2 99.4 44.0 9S, 2

Example 29 to 33

Plants of diploid Boggen variety Voskhod 2 are in the V. Period of Organogeneae (after Kupermann) with a 1% aqueous Jan ulsion of N- (2-dimethylaminoethyl) -alaleamic acid, N- (3-dimethylaminopropyl) -. Maleamic acid, N -Ethylmaleamic acid N- (2-hydroxyethyl) maleamic acid, N-carbamoylmaleamic acid. The emulsion contains 0.1% calcium alkyl benzene sulphonate and 0.01% dimethylformamide. The consumption of protein is 10 kg / ha. As control serve plants, treated with a solvent without sterilizing agent. The test results are, in the table ? shown. The similar results are in the treatment of diploid rye variety Voskhod 2 in the VI. Period of organogenesis obtained.

Table 7

running example no.

1. Control

2. Example 29

3. Example 30

4. Example 31

5. Example 32

6. Example 33

55.2 100 51.2 92.7 48.0 87.0 53.0 96.0 52.2 94.6 51.7 93.7

Number Percentage Percentage of Grains Sterili- um of bodies initiating the act of censoring

A'hre im - "ear of wheat at free Xs oil at free pollination

pollination

49.2 0.0

0,0 100,0

0,0 100,0

0,0 100,0

0.8 98.4

.0,9 98,2

Examples 34 to 38

Plants of diploid rye of the genus Chulpan are in the V. Period of organogenesis (according to Kupermann) with a 1% aqueous emulsion of N- (2-dimethylaminoethyl) -maleamic acid, N- (3-dimethylaminopropyl) -maleamic acid, N-ethylmaleamic acid, N- (2-hydroxyethyl) maleamic acid, tf-Karbamoylmaleaminsäure treated. The emulsion contains 0.01 mass of tetrahydrofuran and 0.1 mass% of calcium alkylbenzenesulfonate having 12 to 20 carbon atoms, per consumption of preparation is 8 kg / ha. As control serve plants, treated with a Verdiinner without sterilizing agent.

The test results are shown in Table 8. The similar results are in the treatment of diploid rye variety Chulpan in the VI. Period of organogenesis obtained.

ZfJStS

Table 8

Ser. example number percentage number percent No. from Kör the steri from Kör sentence of the formality the piecing Ear of corn Ear of wheat from Kör insulator free ners Bestäu free loading advertising dust

1. Control 51 * 2

2. Example 34 0.2

3. Example 35 0.0

4. Example 36 0.3

5. Example 37 0.1

6th example. 39 0.0

0.0 99.6

100.0 99.4 99, θ

100.0

56.8 50.2 51.6 49 »a 50.1 48.7

100.0 88.4 90.8 87.7 88.2 85.7

Examples 39 to 43

Plants of diploid rye variety Talovskaya 12 are in the V. period of the Organogeneee (after Kupermann) with a 1% aqueous emulsion of N- (dimethylaminoethyl) -maleamic acid, N- (3-dimethylatainopropyl) -maleamic acid, N-Äthylmaleaminsäure, N - (2-hydroxyethyl) maleamic acid, N-Karbamoylmaleaminsäure treated. The emulsion contains 0.01 Maaae% Dodezyla al fat and 0.1 mass Kai alkylbenzenesulfonate onat with 12 to 14 carbon atoms. The consumption of preparations is 6 kg / ha. As control serve plants, treated with a thinner without sterilizing agent.

The test results are shown in Table 9. The similar results are in the treatment of diploid rye variety Talovskaya 12 in the VI. Period of the Organogeneae obtained.

Z / S SYS

table 9 number percentage number percentage 100.0 Ser , example from Kör the steri from Kör of the Anset 91.1 No. the formality partners zend of A5.4 Ear of corn in the the ear of wheat Grains 09.1 insulator at free free work 94.7 he is a stubborn man 87.0 advertising 40.2 0.0 56.2 1. control 0.2 99.5 51.2 Second example 39 0.0 100.0 48.0 Third example 40 0.0 100.0 50.1 4 example 41 0.3 99.3 53.2 5th example 42 0.0 100.0 48.9 6th example 43

Examples 44 to 46

Plants of Tritikale variety < Amf idiploid 206 are treated in the V. period of the organogenesis (according to Kupermann) with a 2% aqueous emulsion of N- (3-dimethylaminopropyl) -maleamic acid, N-ethylmaleamic acid, N-carbamoylmaleamic acid.

The emulsion contains 0.01% by weight of dimethylsulfoxide and 0.1% by weight of quinicarbonybenzene sulfonate having 12 to 14 carbon atoms. The consumption of preparation is 12 kg / ha. As control serve plants, treated with a thinner without sterilizing agent.

The test results are shown in Table 10. The similar results are in the treatment of triticae Iorte - Anfidiploid 206 in the VI. Period of organogenesis obtained.

Table 10

running example no.

Number Percentage Number Percentage

of grains of sterol, of grains of corn, of the calves of

In Isola Ähren bei Nern bei frei «

gate open he Beataeu-

Beat exercise

advertising

1. control 44 18.4 O , 0 25.2 100.0 Second example 45 0.1 99 , 5 21.7 86.1 Third example 46 0.4 97 .8th 20.5 ai, 3 4th example 0.0 100 , 0 21.1 83.7

Example 47 and 48

Plants of tritium variety PRAG 109 are treated in the V. period of the organogenes (according to Kupermann) with a 2% aqueous solution of N- (3-dimethylaminopropyl) maleamic acid, N-carbamoylmaleamic acid. The emulsion contains 0.01% tetrahydrofuran and 0.1 wt% of calcium silylbenzenesulfonate having 12 to 14 carbon atoms. The consumption of preparation is 12 kg / ha. As control serve plants, treated with a thinner without sterilizing agent.

The test results are shown in Table 11.

The similar results are in the treatment of the trltical strain PHAG 109 in the VI. Period of organogenesis

receive.

Table 11

running example no.

Number percentage number

of bodies of sterile

the ear of wheat did

percentage

from grains .a of the Anset the ear ►3 zens of at free , 6 grains pollination at free he damned advertising 45, 100.0 40 88.0 39 86.5

1. jion-croiie 47 4 * | 2 U ₁ U Second example 48 O, , 0 100.0 Third example O , 0 100.0

Examples 49 to 55 "

The plants of the millet variety Mironovskoe 94 are in the V. period of organogenesis (after Kupermann) with; a 1% aqueous emulsion of N- (2-dimethylaminoethyl) maleamic acid, N- (3-dimethylaminopropyl) maleamic acid, N-ethylmaleamic acid, N- (2-hydroxyethyl) maleamic acid, N-carbamoylmaleamic acid. The emulsion contains 0.01% dimethyl sulfoxide and 0.1% calcium alkyl benzene sulphonate with 12 to 14 carbon atoms * The consumption of the preparation is 8 kg / ha. As control serve plants, treated with a thinner without sterilizing agent. The test results are shown in Table 12. The similar .Results are in the treatment of millet variety Mironovakoe 94 in the VI. Period of organogenesis obtained.

Table 12

Ser , example 49 number percentage O number percentage No. 50 from Kör the steri , 0 from Kör of piecing 51 the formality , P the of grains 5.2 in the , 0 Panicle at at free 53 insulator "O free pollination , 0 Bestäu advertising 1. control 184.0 0, - 227.3 100.0 Second example 0.0 100 224, a 98.9 Third example 0.0 100 214.5 94.4 4th example 0.0 100 200, a aa, 3 5th example 0.0 100 215.0 94.6 6th example 0.0 100 198.2 87.2

Examples 54 to 53

The plants of the millet variety K-9693 Kormovoe I are in the V * period of organogenesis (after Kupermann) with a

1% aqueous aqueous solution of N- (2-D in a-1-aminoethyl) maleic acid, N- (3-dimethylaminopropyl) nialeamic acid, N-ethylmaleamic acid, N-KaX-bamoylmaleamic acid. The 3 solution contains 0.01 Mass% dimethylformamide and 0.1 wt. KaI-ziumalkylbenzolsulfonat having 12 to 14 carbon atoms.

The consumption of preparation is 6 kg / ha. The following results are shown in the table: The similar results are obtained in the treatment of millet variety K-9693 Kormovoe 1 in the VI * period of the organogenesis.

Table 13

Running example Number Percentage Number Percentage

No, from Körster Sterili- from Kördel Ansetzeng

The cracking action of grains

pe in the panicle at free

Isolator of free pollination

pollination

1. Control 125.0 0.0 146.7 100.0

2. Example 54 0.0 100.0 124.2 84.7

3. Example 55 0.0 100.0 100.8 68.7

4. Example 56 0.0 100.0 114.7 78.2

5. Example 57 0.0 100.0 127.2 86.7

6. Example 58 O ₁ O 100.0 133.6 91.1

Examples 59 to 62

Plants of the sunflower variety Peredovik and line BK -119 are treated in the 5th period of organogenesis (according to Kupermann) with a 0.5% aqueous emulsion of N-carbamoylmaleamic acid. The emulsion contains 0.01% by weight of dimethyl sulfoxide and 0.1 parts by weight of calcium alkylbenzenesulfonate

to 14 carbon atoms. The consumption of preparation is 3 kg / ha. The same plants are treated with an O, lmasseprozentigen aqueous emulsion of N-O-dimethylaminopropyl) - maleamic acid. In the latter case, the emulsion contains 0.01% by weight of dodecyl sulfate and 0.1% by weight of calcium alkylbenzenesulfonate having 12 to 14 carbon atoms. The consumption of preparation is 1 kg / ha. As control serve plants, treated with a thinner without sterilizing agent. The test results are shown in Table 14. Similar results are at treatment of sunflower of the specified varieties in VI. Period of the Organogeneae obtained.

Table 14

Example of the application of the percentage weight of oil-waxer, the rate of Ach- von halt von tums-

Sterilizers at 1000 Achaean energy did fre-% Ach-% in%

dusts in% in g

Variety Peredovik

1. Control 0.2

2nd Example 59 100.0

3. Example 60 100.0

Line BK-119

1. Control 2.0

2nd example 61 100.0

3rd example 64 100.0

85.0 84.0 54.7 100.0 81.7 a?, 7 54.4 100.0 84.9 83.3 54.0 100.0 9 85.0 60.0 51.0 100.0 82.5 5a, 8 50.8 100.0 80.4 59.6 51.0 100.0

Claims (4)

- tz- 1. A method for sterilizing the anthers of plants by their treatment with a sterilizing agent in combination with a thinner in the fifth and / or sixth period of the organogenesis, characterized in that serve as sterilizing agents N-substituted maleamic acids of the general formula RNHCOCH s CHCO ₂ H. in which R (CH ₃ ) ₂ NCH ₂ CH ₂ -; (CH ^ HOH ^ HgCH ^ s C ₃ H ₅ -; HOC ₂ H ₄ -; NH ₃ CO means « 2. The method according to claim 1, characterized in that the stated N-substituted Maleaminsäuren be used in combination with a diluent as a 0.1 to 2 # ige aqueous emulsion. 3. Process according to claims 1, 2, characterized g e k e η n- Suppose that the plants to be treated with the sterilizing agent are grass plants and sunflower. 4. Process according to Claims 1 to 3, characterized in that, in order to achieve a high degree of sterilization of anthers under unfavorable climatic conditions, the repeated treatment of plants with the sterilizing agent takes place in the fifth and / or sixth period of the organogenesis.