DD291237A5 - PROCESS FOR PREVENTING GAEUMANNOMYCES GRAMINIS CONDITIONAL SHEET IN WHEAT PLANTS BY GROWTH STIMULATING BACTERIAL STRAIN - Google Patents

Abstract

The invention relates to a method for preventing Gaeumannomyces graminis-related damage in wheat seedlings by means of a growth-stimulating bacterial strain. The method according to the invention involves the use of the bacterial strain IMET 11423. It demonstrates multiple levels of dry matter, improved germination and improved emergence of young plants. The invention can be used in agricultural crop production. Arthrobacter; Wheat seedling; Gaeumannomyces graminis; More yield; Cytokininbildung; Stimulate growth; emergence; Germination; Dry matter; culture}

Description

Characteristic of the known state of the art

The soil-borne fungus Gaeumannomyces graminis plays a not insignificant role in intensive plant production on light soils. An effective control with fungicides or the cultivation of resistant varieties has never been. t succeeded with corresponding success. Therefore, gesuctit for ways of biological control of the pest. Two groups of organisms are important:

1. Use of bacterial antagonists

2. Preinoculation with non-pathogenic fungi

For agricultural practice there is a method that can be used to control Gaeumannomyces graminis var. Tritici in wheat by means of an antagonistic Phialophora graminicola strain (DD-WP AOIN /

However, this strain still has insufficient results in its performance parameters.

DD-PS 270653 describes a method in which a Pseudomonas putida Starnm is used to combat Gaeumannomyces graminis. Seeds treated in this way yielded higher yields in experiments, caused by a

weakened infestation with Gaeumannomyces graminis. There are still no results available on a broad application of such a method in agricultural practice.

For controlling phytopathogenic soil fungi by means of Pseudomonas putida or unspecified Pseudomonas species using their siderophore production in horticultural crops, EP-PS 0106975, US-PS 4479336, and DE-OS 3335643 have made statements. But they only refer to horticultural crops,

Object of the invention

The object of the invention is the use of a bacterial strain which effectively stimulates the germination and growth of young wheat plants in Gaeumannomyces graminis -infected soil and promotes the growth in length and the dry mass formation of the plants.

Explanation of the essence of the invention

The object of the invention is the use of a bacterial strain as an antagonist against Gaeumannomyces graminis in wheat seedlings. It has been found that the strain Arthrobacter sp. IMET11423 is characterized by a strong inhibition of the growth of Gaeumannomyces gramims isolates in vitro.

The culture filtrate of the bacterial strain ei was tested by a modified barley leaf chlorophyll senescence delay test (MILLER, 1963).

This test represents a detection method of cytokinin equivalents or cytokinin-like compounds excreted into the culture medium during growth of the bacteria. The test is based on the detection of delayed chlorophyll degradation by such compounds in separated barley leaf segments after treatment with the Kulcurfiltrat of the bacterial strain. It could further be stated that the treatment of winter varicose caryopses of the variety 'MIRAS' with the bacterial strain IMET11423 resulted in a significant stimulation of the radicle growth (length) and the shoot (length and dry matter). The highest stimulation effect could be determined at the bacterial concentration of 10 ⁶ cfu / ml, whereby no significant difference was detectable compared to the bacterial densities of 10 ⁷ Gfu / ml and 10 ⁹ cfu / ml.

After the treatment of winter wheat cocxoses of the variety 'MIRAS' with the bacterial strain Arthrobacter sp. IMET11423 was compared to the control a siginfikant shortened soaking period can be determined.

Seed treatment of summer wheat of the variety 'MARIO' and the bacterial strain Arthrobacter sp. IMET11423 continued to stimulate dry matter production of shoot and root in the greenhouse experiment after 90 days of vegetation, as well as increased growth in length despite the high pressure of attack by Gaeumannomyces graminis.

exemplary

In the treatment of wheat seed with the bacterial strain Arthrobacter sp. IMET11423 the following results could be achieved

Table 1

Influence of the bacterial strain IMET11423 on the germination of wheat corymbs of the genus 'MIRAS' In vitro at 27 ⁹ C; Evaluation after 48 hours

  treatment shoot elongation dry matter Length of the Koimwur2eln (Mm) Sprout (mg) (cm) H ₂ O control 7.4 a 15.0 a 12.5 a ¹ Bakterienstamn. IMET1142310 ⁵ cfu / ml 14.9 b 30.8 c 25.6 b 10 ⁷ cfu / ml 13.4 b 27.4 b 23.5 b 10 ⁹ cfu / ml 12.5 b 24.3 b 24.4 b

1 Values with unequal letters indicate significant differences In the Newman-Keuls test at α = 5%

Table 2

Influence of seed treatment with the bacterial strain IMET11423 on the stimulation of the plant emergence in winter wheat of the variety 'MIRAS' in unsterile soil under greenhouse conditions 9 caryopses per vessel designed; η = 5; Evaluation after 5 days

treatment

Number of accumulated plants / vessel

D value (= 0.05)

Control, untreated 4.4

Bacterial strain IMET11423 8.8

2.10 0.55

Table 3

Influence of seed treatment with the bacterial strain IMET11423 on the growth of summer wheat 'MARIO' under greenhouse conditions and present soil infection with Gauumannomyces graminis; η = 10; Vegetation time 90 days

treatment dry matter dry matter plant height Scion (g) Root (g) (cm) Control, untreated soil 4.91 0.47 67.6 Control, soil infected m. G. graminis 3.43 0.32 69.6 Seed treatment with IMET11423 and G.grarninis infection 5.70 0.41 73.6 LSD (a = 0.05) 0.98 0.11 5.5

Table 4

Chlorophyll content in barley leaf sections (extracted in ethanol) after treatment with the culture filtrate of the bacterial strain IMET11423 (extinction at 665nm)

treatment

Absorbance at 665 nm

Control (Medium) 0.468 a ¹

Bacterial strain IMET11423 0.632 b

1 means with unequal letters characterize the significant difference in Student-Newman-Keuls test (α = 5%)

Claims (1)

Method for preventing Gaeumannomyces graminis-related damage in wheat seedlings by means of a growth-stimulating bacterial strain, characterized in that the seed before sowing with the bacterial strain Arthrobacter sp. IMET11423 is treated. Field of application of the invention The invention relates to a method for preventing Gaeumannomyces graminis-related damage in wheat seedlings by means of growth-stimulating bacterial strain. It can be used in agricultural crop production,