DD287193A5 - METHOD OF RESTRICTING GAEUMANNOMYCES GRAMINIS VAR. TRITICI IN WHEAT BY A PRESCHOKULATION BY PHIALOPHORN SP. (LOBED HYPHOPODIA) - Google Patents

Abstract

The invention relates to a method for restricting Gaeumannomyces graminis by means of a preinoculation of Phialophora sp. (lobed hyphopodia) IMET 43910. In wheat, increased dry-matter masses and grain traits were detected by application of this method {Phialophora sp. (lobed hyphopodia) antagonists; Wheat; More yield; Soil fungi; Praeinokulation}

Description

Characteristics' - known prior art

The soil-borne fungus Gaeumannomyces graminis plays a not insignificant role in the further intensification of cereal production as a harmful factor (STEINBRENNER, 1987). An effective control with fungicides or resistance breeding has hitherto been successful with corresponding success. Therefore, we are looking for ways of biological control. Two groups of organisms are important:

1. Use of bacterial antagonists

2. Preinoculation of apathogenic fungi.

Such non-pathogenic fungi are Phialophora graminicola (SCOTT, 1970, BALIS, 1970, DEACON, 1973, 1974, 1976); Phialophora sp. (Lobed hyphopodia) (DEACON, 1974, 1976); Phialophora spp. (SIVASITMANPARAM, 1975); hypovirulent strains of the pathogen (HVOLL 6t al., 1974) and Gaeumannomyces graminis var. graminis (WONG, 1975). Similarly, working groups in Great Biitania and Australia, which are concerned with the use of Phialophora isolates in cereals and grasslands, have had a positive impact on damage compensation of Gaeumannomyces graminis to the 2nd wheat replant as a result of their enrichment by the forefoot grass clover mixture (PREW, 1981) or ryegrass-legume mixture (SLOPE et al.,

The statements refer to Phialophora isolates without further characterization of the strains.

For agricultural practice, there is a method that can be used for controlling Gaeumannomyces graminis var. Tritici in wheat by means of an antagonistic Phialophora graminicola strain (DD-WP A01N 3237463). However, this strain still has insufficient results in its performance parameters.

Object of the invention

The aim of the invention is the use of a strain of Pnialophora sp. (Lobed hyphopodia), which effectively limits the infestation of wheat plants by Gaeumannomyces graminis var. tritici and has a positive influence on the growth stimulation.

Explanation of the essence of the invention

The object of the invention is the use of a Phialophoru jp. (lobed hyphopodlal strain? as an antagonist for Gaemannomyces graminis var. tritici in wheat.

It was found that the strain Phialophora sp. (lobed hyphopodia) IMET 43910 in the pathogenicity test characterized by a positive effect on the development of young plants (harvest after 6 weeks).

The testing of the fungal isolates selected for pre-inoculation was carried out in the two-stage test system.

In a preinoculation of phialophr ca sp. (lobed hyphopodia) IMET 43910 two to three weeks before infection with mi ', an aggressive strain of pathogens, was able to completely prevent the harmful effects of this strain with marked pathogenicity.

At the same time, there were growth-promoting effects compared to uninfected control.

This observation does not change with only weak pathogenicity of the control strain.

At the same time bringing together the roots with the pathogen and the Philophora sp. In both cases of different concentration ratios (lobed hyphopodia) isolate IMET 43910, it is possible to compensate for the harmful effects in the six-week old plants compared to the infected control variant.

Again, a lessening aggressiveness of the control strain was able to demonstrate a growth-stimulating effect.

Similar to the model tests in the climate chamber under semi-sterile conditions, experiments were carried out in which the plant cultivation took place in substrate which was inoculated simultaneously and uniformly with the Präinokulationspartnern and fungal strains. Planting and observation took place in the greenhouse and field during a full growing season with unsterile soil.

Even under these conditions, the promoting effect of deep isolate IMET 43910 on plant growth and thus a reduction in the harmful effects of highly virulent strains of Gaeumannomyces graminis could be observed. The level of uninfected control could be exceeded by an average of 45% in the sprouted solids parameters and by an average of 59% in the grain yield.

embodiment The invention will be illustrated by the following explanations.

!. Preliminary or model trial under semi-sterile and climatically controllable conditions in the phytotron (substrate, quartz sand). The Pflanzenernto took place after a developmental period of 6 weeks, with the evaluation parameters Sproßtrockenmasse, root dry matter, root drain and root length served. 2. Gafasverouche in the greenhouse and field (soil from uncontaminated experimental plots). The harvest took place after one growing season.

In the model experiment, the time to perform a pre-inoculation test was used as a test factor.

A - Cultivation of the plants in substrate, which was only added to those used for pre-inoculation fungal strains. After reaching the stage of development of the plants DC 13 (according to ZADOK), these were transformed into substrate which had been inoculated with the strongly aggressive fungal strains.

B - Cultivation of the plants in substrate, which was inoculated simultaneously and evenly with the Präinokulationspartnern and aggressive fungal strains. In this stage B, the factor concentration ratio between preinoculation partner and aggressive control strain (B / 1 = 1: 1, B / 2 = 3: 1) was also tested. In the further investigations in the greenhouse only the variants B / 1 and B / 2 were tested in approximation to the practice.

example 1 Influence of Phiaiophora sp. (Iobed hyphopodia) IMET 43910 on semi-sterile seedling development of wheat plants test

Handling variant Sprout dry matter (g) Root-root-i dry mass low (g) (cm) 15.5 21.1 + Root length (cm) Main roots In addition to roots Sprout mass (g) Culm length (cm) Ears length (cm) Grain number / ear Grain yield / pot (g) TKM (g) uninfected control + IMET 43910 0.09 0.11 + 0.04 0.09 3.3 603.0 1 288.0 9.2 10.1 r 113.3 261.3+ 2.3 3.2+ 59.2 62.8 10.3 11.0 38.3 44.4 7.2 11,6+ 38.1 42.1 LSD (α = 0.05) 0.02 0.06 228.6 1.4 53,5+ 0.6 5.6 1.2 10.2 4.0 0.2 Example 2 Influence of Phiaiophora sp. (Iobed hyphopodia) IMET 43910 on the plant yield of wheat in the unsterile plant test Treatment var. uninfected control + IMET 43910 LSDYa = 0.05)

+ = significantly superior.

Effect of Preinoculation of Phiaiophora sp. (Iobed hyphopodia) IMET 43910 on damage to young seedlings of wheat by Gaeumannomyces graminis in semisterial plant test (test variant A)

example 1

Behandlungsvailante shoot dry root dry root length Root draft mass (g) mass (g) (cm) (cm) nlchtinfizierte control 0.09 ν + 0.04 ν 603.0 ν + 15.5 ν + with G.graminis (G 46) infected control 0.03 - ν 0.02 ν 193.6 - ν 10,9 - ν G 46 + IMET 43910 0.12 + + 0.08 817.6 + 21.4 + + LSD (= 0.05) 0.02 0.07 229.8 2.9

ν = Verglelchavarianle

 + = significantly superior

 - = Signifikani inferior.

Example 2

treatment option shoot dry root dry Wurzeliänge Root draft mass (g) mass (g) (cm) (cm) uninfected control 0.09 ν 0.04 ν 603.0 ν 15.5 BC with G. graminis (G 41! Infected 0.08 ν 0,05 ν 644.4 ν 15.3 BC control G 41 + IMET 43910 0.14 + + 0.09 + + 731.3 19.9 + + LSD (= 0.05) 0.02 0.02 236.1 3.1 ν = comparison variant. + = significantly superior. - = significantly inferior Example 3 uninfected control 0.09 ν + 0.04 ν 603.0 ν + 15.5 ν + with G. graminis (G 38) infected 0.04 - ν 0.01 ν 147.8 - ν 4,5 - ν control G38 + IMET 43910 0.14 + + 0.14 808.8 + + 16.7 + LSD (α = 0.05) 0.02 0.07 184.5 2.4

ν = comparison variant. + = significantly superior. - = significantly inferior

Effect of Preinoculation of Phialophora sp. (lobed hyphopodia) IMET 43910 on dio damage of the young plants of wheat by Gaeumannomyces graminis in the semi-sterile plant test (test variant B / 1)

example 1

treatment option Sprouted mass (g) Root dry mass (g) Root length (cm) Root barrel (cm) uninified control with G. graminis (G 46) infected control G46 + IMET 43910 0.20 ν + 0.10 - ν J, 13 - + 0.13 ν + 0.04 - ν 0.10 - + 1005.6 ν + 206.1 - ν 425.8 - + 24..0 ν + 9.1 - V 17.9 - + LSD (= 0.05) 0.03 0.02 127.1 2.9 Example 2 uninfected control with G. graminis (G41) infected control G41 + IMET 43910 0.17 ν + 0.06 - ν 0.13 + 0.09 ν + 0.02 - ν 0.06 - + 345 ν + 104.1 - ν 490.1 + + 18.3 ν + 10.2 - ν 18.4 + LSD (O. = 0.05) 0.06 0.02 61.7 2.6 Example 3 uninfected control with Q. graminis (Q 38) Infected control G38 + IMET 43910 0.13 ν - 0.21 + ν 0.25 + + 0.06 ν - 0.13 + ν 0.13 + 735.9 ν - 1223.4 + V 1007.4 + 22.0 ν 24.1 V 25.7 + LSD (α - = 0.05) 0.03 0.02 216.1 2.9

Effect of Preinoculation of Phiaiophora sp. (Iobed hyphopodia) IMET 43910 on Damage to Young Plants of Wheat by Gaeumaiinomyces graminis in the Semi-Sterile Plant Test (Experimental B / 2)

example 1

treatment option Sprouted mass (g) Root dry mass (g) Root length (cm) Root barrel (cm) uninfected control with G.graminis (G46) infected control G46 + IMET 43910 0.15 ν + 0.10 - ν 0.14 + 0.08 ν + 0.04 - ν 0.09 + 805.7 ν + 567.8 - ν 786.6 + 19,6 ν + 12,0 - ν 21,4 + LSD (O = 0.05) 0.03 0.04 188.1 2.9 Example 2 uninfected control with G. graminis (G 41! infected control G41 + IMET 43910 0.15 ν + 0.09 - ν 0.18 + + 0.08 ν + 0.03 ν 0.09 805.7 ν + 411.1 - ν 1071.2 + + 19,6 ν + 7,1 - ν 20,6 + LSD (α = 0.05) 0.03 0.09 214.5 2.8 Example 3 uninfected control with G. graminis (G 38) infected control G38 + IMET 43910 0.15 ν + 0.09 - ν 0.20 + + 0.08 ν + 0.04 - ν 0.12 + + 605.7 ν + 448.3 - ν 774.2 + 19,6 ν + 19,6 ν 18,1 LSD (= 0.05) 0.04 0.02 237.7 3.2

Effect of Preinoculation of Phiaiophora sp. (lobed hyphopodia) IMET 43910 on the damage of wheat plants by Gaeumannomyces graminis in the unsterile plant test (test variant B / 1 and B / 2)

example 1

treatment option Sprouted mass) Half length (cm) Grain yield / pot (g) TKM (g) Grain number / spike non-verified control with G. graminis (G 46/41! infected 'G46 / 41 + IMET 43910 2.2 ν + 0.8 - ν 2.6 + 60.3 ν + 37.6 - ν 60.1 + 2.9 ν + 0.3 - ν 3.6 + 41.6 ν + 17.1 - ν 41.2 + 24.2 ν + 5.3 - ν 31.0 + LSD (α = 0.05) 0.6 7.1 1.2 8.0 8.1 Example 2 noninfected control with G. graminis (G 46/41) infected control G46 / 41 + IMET 43910 2.2 ν + 0.8 - ν 3.2 + + 60.3 ν + 37.6 - ν 63.4 + 2.9 ν + 0.3 - ν 4,6 + + 41.6 ν + 17.1 - ν 44.3 + 24.2 ν + 5.3 - ν 33.6 + + LSD (Q = 0.05) 0.4 5.4 0.9 8.0 6.1

Claims (1)

A process for the restriction of Gaeumannomyces graminis var. Tritici on wheat, characterized in that the culture substrate or the plant culture iur genus Phialopnora ερ. (lobed hyphopodia) belonging to mushroom isolate IMET 43910 is added. Field of application of the invention The invention relates to a method for controlling Gaeumannomyces graminis var. Tritici by means of a preinoculation of Fhlalophora sp. (lobed hyphopodia) in wheat. It can be used in plant production.