DE10347311A1 - Verfahren und Vorrichtung zur Durchführung von Reaktionen - Google Patents

Verfahren und Vorrichtung zur Durchführung von Reaktionen Download PDF

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Publication number
DE10347311A1
DE10347311A1 DE2003147311 DE10347311A DE10347311A1 DE 10347311 A1 DE10347311 A1 DE 10347311A1 DE 2003147311 DE2003147311 DE 2003147311 DE 10347311 A DE10347311 A DE 10347311A DE 10347311 A1 DE10347311 A1 DE 10347311A1
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Prior art keywords
reaction
carrying
out reactions
time
path
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DE2003147311
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Inventor
Wolfgang Ehrfeld
Karoly Nagy
Alexander Azzawi
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Ehrfeld Mikrotechnik BTS GmbH
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EHRFELD MIKROTECHNIK AG
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Priority to DE2003147311 priority Critical patent/DE10347311A1/de
Priority to EP04765899A priority patent/EP1673161A1/de
Priority to PCT/EP2004/011291 priority patent/WO2005035110A1/de
Publication of DE10347311A1 publication Critical patent/DE10347311A1/de
Withdrawn legal-status Critical Current

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    • BPERFORMING OPERATIONS; TRANSPORTING
    • B82NANOTECHNOLOGY
    • B82YSPECIFIC USES OR APPLICATIONS OF NANOSTRUCTURES; MEASUREMENT OR ANALYSIS OF NANOSTRUCTURES; MANUFACTURE OR TREATMENT OF NANOSTRUCTURES
    • B82Y30/00Nanotechnology for materials or surface science, e.g. nanocomposites
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B01PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01JCHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
    • B01J19/00Chemical, physical or physico-chemical processes in general; Their relevant apparatus
    • B01J19/0046Sequential or parallel reactions, e.g. for the synthesis of polypeptides or polynucleotides; Apparatus and devices for combinatorial chemistry or for making molecular arrays
    • BPERFORMING OPERATIONS; TRANSPORTING
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    • B01LCHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
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    • G01N35/10Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
    • G01N35/1065Multiple transfer devices
    • G01N35/1074Multiple transfer devices arranged in a two-dimensional array
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    • C40COMBINATORIAL TECHNOLOGY
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  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Nanotechnology (AREA)
  • Organic Chemistry (AREA)
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  • Composite Materials (AREA)
  • Crystallography & Structural Chemistry (AREA)
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  • Condensed Matter Physics & Semiconductors (AREA)
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  • Biochemistry (AREA)
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Abstract

Die Erfindung betrifft eine Vorrichtung zur Durchführung von Reaktionen mit Reagenzmengen im Bereich von 0,5 bis 10 nmol, bei der die Reagenzien über Dosierstationen (5, 9, 11, 13) an Reaktionsorte (3) dosiert werden, die sich entlang einer Reaktionsstrecke (2) bewegen, wobei mindestens eine Dosierstation (5, 9, 11, 13) und eine Entnahmestelle (16) derart angeordnet sind, dass die Zeit, in der sich die Reaktionsorte (3) auf der Reaktionsstrecke (2) bewegen, mindestens der erforderlichen Reaktionszeit für einen Reaktionsschritt entspricht.
DE2003147311 2003-10-08 2003-10-08 Verfahren und Vorrichtung zur Durchführung von Reaktionen Withdrawn DE10347311A1 (de)

Priority Applications (3)

Application Number Priority Date Filing Date Title
DE2003147311 DE10347311A1 (de) 2003-10-08 2003-10-08 Verfahren und Vorrichtung zur Durchführung von Reaktionen
EP04765899A EP1673161A1 (de) 2003-10-08 2004-10-08 Verfahren und vorrichtung zur durchführung von reaktionen
PCT/EP2004/011291 WO2005035110A1 (de) 2003-10-08 2004-10-08 Verfahren und vorrichtung zur durchführung von reaktionen

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Application Number Priority Date Filing Date Title
DE2003147311 DE10347311A1 (de) 2003-10-08 2003-10-08 Verfahren und Vorrichtung zur Durchführung von Reaktionen

Publications (1)

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DE10347311A1 true DE10347311A1 (de) 2005-05-12

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DE2003147311 Withdrawn DE10347311A1 (de) 2003-10-08 2003-10-08 Verfahren und Vorrichtung zur Durchführung von Reaktionen

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EP (1) EP1673161A1 (de)
DE (1) DE10347311A1 (de)
WO (1) WO2005035110A1 (de)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114206891A (zh) * 2019-04-10 2022-03-18 日东电工艾维西亚有限公司 用于生物聚合物的顺序合成的方法和设备

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2005058471A2 (en) * 2003-12-15 2005-06-30 Applera Corporation Automated oligomer synthesis
WO2009134821A1 (en) * 2008-04-28 2009-11-05 Douglas Machine, Inc. High throughput screening employing combination of dispensing well plate device and array tape
CA2906011A1 (en) * 2013-03-15 2014-09-18 Douglas Scientific, LLC Reusable belt with a matrix of wells
JP2026506529A (ja) * 2023-02-01 2026-02-25 アローヘッド ファーマシューティカルズ インコーポレイテッド オリゴマー合成のためのベルトリアクター製造方法

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5310674A (en) * 1982-05-10 1994-05-10 Bar-Ilan University Apertured cell carrier
WO1997019749A1 (en) * 1995-11-27 1997-06-05 Dehlinger Peter J Method and apparatus for producing position-addressable combinatorial libraries
WO2000040330A2 (de) * 1998-12-30 2000-07-13 Mwg - Biotech Ag Vorrichtung zur durchführung von chemischen reaktionen
DE60000539T2 (de) * 1999-04-16 2003-03-06 Pe Corp Ny Foster City Vorrichtung und verfahren zur übertragung von kleinen substanzvolumen
DE60000874T2 (de) * 1999-02-16 2003-08-28 Pe Corp. (Ny), Foster City Matrixsystem für aufbewahrung und abgabe

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2353856A1 (fr) * 1976-06-02 1977-12-30 Chateau Guy Ruban destine a servir de support a une reaction par exemple chimique ou biochimique, et procede d'analyse le mettant en oeuvre
US5985214A (en) * 1997-05-16 1999-11-16 Aurora Biosciences Corporation Systems and methods for rapidly identifying useful chemicals in liquid samples
AU5126400A (en) * 1999-05-03 2000-11-17 Ljl Biosystems, Inc. Integrated sample-processing system
WO2001077640A2 (en) * 2000-04-05 2001-10-18 Alexion Pharmaceuticals, Inc. Methods and devices for storing and dispensing liquids
US6841663B2 (en) * 2001-10-18 2005-01-11 Agilent Technologies, Inc. Chemical arrays

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5310674A (en) * 1982-05-10 1994-05-10 Bar-Ilan University Apertured cell carrier
WO1997019749A1 (en) * 1995-11-27 1997-06-05 Dehlinger Peter J Method and apparatus for producing position-addressable combinatorial libraries
WO2000040330A2 (de) * 1998-12-30 2000-07-13 Mwg - Biotech Ag Vorrichtung zur durchführung von chemischen reaktionen
DE60000874T2 (de) * 1999-02-16 2003-08-28 Pe Corp. (Ny), Foster City Matrixsystem für aufbewahrung und abgabe
DE60000539T2 (de) * 1999-04-16 2003-03-06 Pe Corp Ny Foster City Vorrichtung und verfahren zur übertragung von kleinen substanzvolumen

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
CHENG,Ji-Yen,et.al.: High throughput parallel synthesis of oligonu- cleotides with 1536 channel synthesizer. In: Nucleic Acids Research, 2002,Vol.30,No.18,e93 *

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114206891A (zh) * 2019-04-10 2022-03-18 日东电工艾维西亚有限公司 用于生物聚合物的顺序合成的方法和设备
EP3953364A4 (de) * 2019-04-10 2023-01-11 NITTO DENKO Avecia Inc. Verfahren und vorrichtung zur sequenziellen synthese von biologischen polymeren
US12239950B2 (en) 2019-04-10 2025-03-04 Nitto Denko Avecia, Inc. Process and apparatus for sequential synthesis of biological polymers

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