DE602004018155D1 - Einen grössenausschlussschritt verwendendes verbessertes bisulfit-verfahren - Google Patents

Einen grössenausschlussschritt verwendendes verbessertes bisulfit-verfahren

Info

Publication number
DE602004018155D1
DE602004018155D1 DE602004018155T DE602004018155T DE602004018155D1 DE 602004018155 D1 DE602004018155 D1 DE 602004018155D1 DE 602004018155 T DE602004018155 T DE 602004018155T DE 602004018155 T DE602004018155 T DE 602004018155T DE 602004018155 D1 DE602004018155 D1 DE 602004018155D1
Authority
DE
Germany
Prior art keywords
bisulfit
improved
size exclusion
exclusion step
nucleic acid
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE602004018155T
Other languages
English (en)
Inventor
Victoria L Boyd
Gerald Zon
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Applied Biosystems LLC
Original Assignee
Applied Biosystems Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=34278642&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=DE602004018155(D1) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Applied Biosystems Inc filed Critical Applied Biosystems Inc
Publication of DE602004018155D1 publication Critical patent/DE602004018155D1/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Glass Compositions (AREA)
DE602004018155T 2003-08-29 2004-08-27 Einen grössenausschlussschritt verwendendes verbessertes bisulfit-verfahren Expired - Lifetime DE602004018155D1 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US49899603P 2003-08-29 2003-08-29
US52094103P 2003-11-17 2003-11-17
PCT/US2004/028089 WO2005021803A1 (en) 2003-08-29 2004-08-27 Improved bisulfite method employing size exclusion step

Publications (1)

Publication Number Publication Date
DE602004018155D1 true DE602004018155D1 (de) 2009-01-15

Family

ID=34278642

Family Applications (1)

Application Number Title Priority Date Filing Date
DE602004018155T Expired - Lifetime DE602004018155D1 (de) 2003-08-29 2004-08-27 Einen grössenausschlussschritt verwendendes verbessertes bisulfit-verfahren

Country Status (8)

Country Link
US (1) US7262013B2 (de)
EP (1) EP1658383B1 (de)
JP (1) JP2007503813A (de)
AT (1) ATE416262T1 (de)
AU (1) AU2004269393B2 (de)
CA (1) CA2537183A1 (de)
DE (1) DE602004018155D1 (de)
WO (1) WO2005021803A1 (de)

Families Citing this family (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20060134643A1 (en) 2000-06-19 2006-06-22 Kurt Berlin Bisulfite conversion of DNA
US7371526B2 (en) * 2003-08-29 2008-05-13 Applera Corporation Method and materials for bisulfite conversion of cytosine to uracil
US7368239B2 (en) * 2003-08-29 2008-05-06 Applera Corporation Method and materials for polyamine catalyzed bisulfite conversion of cytosine to uracil
US7534873B2 (en) * 2003-08-29 2009-05-19 Applied Biosystems, Llc Method and materials for quaternary amine catalyzed bisulfite conversion of cytosine to uracil
ES2432025T3 (es) * 2003-10-09 2013-11-29 Epigenomics Ag Conversión mejorada de ADN con bisulfito
AU2005286831A1 (en) * 2004-09-21 2006-03-30 Applied Biosystems, Llc. Methods of using sulfur nucleophiles as improved alternatives to sodium bisulfite for methylated DNA analysis
US20060286577A1 (en) * 2005-06-17 2006-12-21 Xiyu Jia Methods for detection of methylated DNA
DE102005034628B4 (de) * 2005-07-19 2007-08-23 Epigenomics Ag Verfahren zur Untersuchung von Cytosin-Methylierungen in DNA
WO2007024800A2 (en) 2005-08-22 2007-03-01 Applera Corporation Device and method for making discrete volumes of a first fluid in contact with a second fluid, which are immiscible with each other
JP2008136404A (ja) * 2006-11-30 2008-06-19 Sysmex Corp Dnaメチル化検出における非メチル化シトシン変換処理後のdna量の確認方法
US20080213870A1 (en) * 2007-03-01 2008-09-04 Sean Wuxiong Cao Methods for obtaining modified DNA from a biological specimen
WO2010003153A2 (en) * 2008-07-03 2010-01-07 Life Technologies Corporation Methylation analysis of mate pairs
CA2921234A1 (en) * 2013-09-30 2015-04-02 Us Biomarkers, Inc. Biomarkers for detection of colorectal cancer
WO2024044764A2 (en) * 2022-08-26 2024-02-29 University Of Virginia Patent Foundation Microfluidic system and method for dna methylation sample preparation

Family Cites Families (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPH07265082A (ja) 1994-03-28 1995-10-17 Makoto Komiyama 核酸を化学変換する方法
US6251594B1 (en) 1997-06-09 2001-06-26 Usc/Norris Comprehensive Cancer Ctr. Cancer diagnostic method based upon DNA methylation differences
DE19754482A1 (de) 1997-11-27 1999-07-01 Epigenomics Gmbh Verfahren zur Herstellung komplexer DNA-Methylierungs-Fingerabdrücke
US6331393B1 (en) 1999-05-14 2001-12-18 University Of Southern California Process for high-throughput DNA methylation analysis
DE10029915B4 (de) 2000-06-19 2005-06-16 Epigenomics Ag Verfahren zum Nachweis von Cytosin-Methylierungen
JP2004507230A (ja) 2000-07-03 2004-03-11 アプレラ コーポレイション ポリヌクレオチド配列アッセイ
DE10050942B4 (de) 2000-10-10 2005-11-17 Epigenomics Ag Verfahren zum Nachweis von Cytosin-Methylierungen
AU1312502A (en) 2000-10-11 2002-04-22 Pe Corp Ny Fluorescent nucleobase conjugates having anionic linkers
DE10151055B4 (de) 2001-10-05 2005-05-25 Epigenomics Ag Verfahren zum Nachweis von Cytosin-Methylierung in CpG Inseln
US9394332B2 (en) * 2002-08-29 2016-07-19 Epigenomics Ag Method for bisulfite treatment
WO2004067545A1 (en) 2003-01-29 2004-08-12 Roche Diagnostics Gmbh Improved method for bisulfite treatment
US7371526B2 (en) 2003-08-29 2008-05-13 Applera Corporation Method and materials for bisulfite conversion of cytosine to uracil

Also Published As

Publication number Publication date
US7262013B2 (en) 2007-08-28
AU2004269393A1 (en) 2005-03-10
JP2007503813A (ja) 2007-03-01
EP1658383B1 (de) 2008-12-03
AU2004269393B2 (en) 2010-08-19
WO2005021803A1 (en) 2005-03-10
ATE416262T1 (de) 2008-12-15
CA2537183A1 (en) 2005-03-10
US20050079527A1 (en) 2005-04-14
EP1658383A1 (de) 2006-05-24

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Legal Events

Date Code Title Description
8327 Change in the person/name/address of the patent owner

Owner name: APPLIED BIOSYSTEMS,LLC (N. D. GES. D. STAATES , US

8363 Opposition against the patent
8380 Miscellaneous part iii

Free format text: PFANDRECHT

8380 Miscellaneous part iii

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