DE602004018155D1 - Einen grössenausschlussschritt verwendendes verbessertes bisulfit-verfahren - Google Patents
Einen grössenausschlussschritt verwendendes verbessertes bisulfit-verfahrenInfo
- Publication number
- DE602004018155D1 DE602004018155D1 DE602004018155T DE602004018155T DE602004018155D1 DE 602004018155 D1 DE602004018155 D1 DE 602004018155D1 DE 602004018155 T DE602004018155 T DE 602004018155T DE 602004018155 T DE602004018155 T DE 602004018155T DE 602004018155 D1 DE602004018155 D1 DE 602004018155D1
- Authority
- DE
- Germany
- Prior art keywords
- bisulfit
- improved
- size exclusion
- exclusion step
- nucleic acid
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- LSNNMFCWUKXFEE-UHFFFAOYSA-N Sulfurous acid Chemical compound OS(O)=O LSNNMFCWUKXFEE-UHFFFAOYSA-N 0.000 title 1
- 230000007717 exclusion Effects 0.000 title 1
- LSNNMFCWUKXFEE-UHFFFAOYSA-M Bisulfite Chemical compound OS([O-])=O LSNNMFCWUKXFEE-UHFFFAOYSA-M 0.000 abstract 1
- 150000007523 nucleic acids Chemical class 0.000 abstract 1
- 102000039446 nucleic acids Human genes 0.000 abstract 1
- 108020004707 nucleic acids Proteins 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Analytical Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Immunology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Saccharide Compounds (AREA)
- Glass Compositions (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US49899603P | 2003-08-29 | 2003-08-29 | |
| US52094103P | 2003-11-17 | 2003-11-17 | |
| PCT/US2004/028089 WO2005021803A1 (en) | 2003-08-29 | 2004-08-27 | Improved bisulfite method employing size exclusion step |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| DE602004018155D1 true DE602004018155D1 (de) | 2009-01-15 |
Family
ID=34278642
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DE602004018155T Expired - Lifetime DE602004018155D1 (de) | 2003-08-29 | 2004-08-27 | Einen grössenausschlussschritt verwendendes verbessertes bisulfit-verfahren |
Country Status (8)
| Country | Link |
|---|---|
| US (1) | US7262013B2 (de) |
| EP (1) | EP1658383B1 (de) |
| JP (1) | JP2007503813A (de) |
| AT (1) | ATE416262T1 (de) |
| AU (1) | AU2004269393B2 (de) |
| CA (1) | CA2537183A1 (de) |
| DE (1) | DE602004018155D1 (de) |
| WO (1) | WO2005021803A1 (de) |
Families Citing this family (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20060134643A1 (en) | 2000-06-19 | 2006-06-22 | Kurt Berlin | Bisulfite conversion of DNA |
| US7371526B2 (en) * | 2003-08-29 | 2008-05-13 | Applera Corporation | Method and materials for bisulfite conversion of cytosine to uracil |
| US7368239B2 (en) * | 2003-08-29 | 2008-05-06 | Applera Corporation | Method and materials for polyamine catalyzed bisulfite conversion of cytosine to uracil |
| US7534873B2 (en) * | 2003-08-29 | 2009-05-19 | Applied Biosystems, Llc | Method and materials for quaternary amine catalyzed bisulfite conversion of cytosine to uracil |
| ES2432025T3 (es) * | 2003-10-09 | 2013-11-29 | Epigenomics Ag | Conversión mejorada de ADN con bisulfito |
| AU2005286831A1 (en) * | 2004-09-21 | 2006-03-30 | Applied Biosystems, Llc. | Methods of using sulfur nucleophiles as improved alternatives to sodium bisulfite for methylated DNA analysis |
| US20060286577A1 (en) * | 2005-06-17 | 2006-12-21 | Xiyu Jia | Methods for detection of methylated DNA |
| DE102005034628B4 (de) * | 2005-07-19 | 2007-08-23 | Epigenomics Ag | Verfahren zur Untersuchung von Cytosin-Methylierungen in DNA |
| WO2007024800A2 (en) | 2005-08-22 | 2007-03-01 | Applera Corporation | Device and method for making discrete volumes of a first fluid in contact with a second fluid, which are immiscible with each other |
| JP2008136404A (ja) * | 2006-11-30 | 2008-06-19 | Sysmex Corp | Dnaメチル化検出における非メチル化シトシン変換処理後のdna量の確認方法 |
| US20080213870A1 (en) * | 2007-03-01 | 2008-09-04 | Sean Wuxiong Cao | Methods for obtaining modified DNA from a biological specimen |
| WO2010003153A2 (en) * | 2008-07-03 | 2010-01-07 | Life Technologies Corporation | Methylation analysis of mate pairs |
| CA2921234A1 (en) * | 2013-09-30 | 2015-04-02 | Us Biomarkers, Inc. | Biomarkers for detection of colorectal cancer |
| WO2024044764A2 (en) * | 2022-08-26 | 2024-02-29 | University Of Virginia Patent Foundation | Microfluidic system and method for dna methylation sample preparation |
Family Cites Families (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH07265082A (ja) | 1994-03-28 | 1995-10-17 | Makoto Komiyama | 核酸を化学変換する方法 |
| US6251594B1 (en) | 1997-06-09 | 2001-06-26 | Usc/Norris Comprehensive Cancer Ctr. | Cancer diagnostic method based upon DNA methylation differences |
| DE19754482A1 (de) | 1997-11-27 | 1999-07-01 | Epigenomics Gmbh | Verfahren zur Herstellung komplexer DNA-Methylierungs-Fingerabdrücke |
| US6331393B1 (en) | 1999-05-14 | 2001-12-18 | University Of Southern California | Process for high-throughput DNA methylation analysis |
| DE10029915B4 (de) | 2000-06-19 | 2005-06-16 | Epigenomics Ag | Verfahren zum Nachweis von Cytosin-Methylierungen |
| JP2004507230A (ja) | 2000-07-03 | 2004-03-11 | アプレラ コーポレイション | ポリヌクレオチド配列アッセイ |
| DE10050942B4 (de) | 2000-10-10 | 2005-11-17 | Epigenomics Ag | Verfahren zum Nachweis von Cytosin-Methylierungen |
| AU1312502A (en) | 2000-10-11 | 2002-04-22 | Pe Corp Ny | Fluorescent nucleobase conjugates having anionic linkers |
| DE10151055B4 (de) | 2001-10-05 | 2005-05-25 | Epigenomics Ag | Verfahren zum Nachweis von Cytosin-Methylierung in CpG Inseln |
| US9394332B2 (en) * | 2002-08-29 | 2016-07-19 | Epigenomics Ag | Method for bisulfite treatment |
| WO2004067545A1 (en) | 2003-01-29 | 2004-08-12 | Roche Diagnostics Gmbh | Improved method for bisulfite treatment |
| US7371526B2 (en) | 2003-08-29 | 2008-05-13 | Applera Corporation | Method and materials for bisulfite conversion of cytosine to uracil |
-
2004
- 2004-08-26 US US10/926,534 patent/US7262013B2/en not_active Expired - Fee Related
- 2004-08-27 WO PCT/US2004/028089 patent/WO2005021803A1/en not_active Ceased
- 2004-08-27 EP EP04782547A patent/EP1658383B1/de not_active Revoked
- 2004-08-27 DE DE602004018155T patent/DE602004018155D1/de not_active Expired - Lifetime
- 2004-08-27 AT AT04782547T patent/ATE416262T1/de not_active IP Right Cessation
- 2004-08-27 AU AU2004269393A patent/AU2004269393B2/en not_active Expired - Fee Related
- 2004-08-27 CA CA002537183A patent/CA2537183A1/en not_active Abandoned
- 2004-08-27 JP JP2006524923A patent/JP2007503813A/ja active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| US7262013B2 (en) | 2007-08-28 |
| AU2004269393A1 (en) | 2005-03-10 |
| JP2007503813A (ja) | 2007-03-01 |
| EP1658383B1 (de) | 2008-12-03 |
| AU2004269393B2 (en) | 2010-08-19 |
| WO2005021803A1 (en) | 2005-03-10 |
| ATE416262T1 (de) | 2008-12-15 |
| CA2537183A1 (en) | 2005-03-10 |
| US20050079527A1 (en) | 2005-04-14 |
| EP1658383A1 (de) | 2006-05-24 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| 8327 | Change in the person/name/address of the patent owner |
Owner name: APPLIED BIOSYSTEMS,LLC (N. D. GES. D. STAATES , US |
|
| 8363 | Opposition against the patent | ||
| 8380 | Miscellaneous part iii |
Free format text: PFANDRECHT |
|
| 8380 | Miscellaneous part iii |
Free format text: PFANDRECHT AUFGEHOBEN |