DE60217393D1 - Verfahren zur kombinierten parallelen zuführung von agentien und elektroporation für zellstrukturen und verwendung davon - Google Patents

Verfahren zur kombinierten parallelen zuführung von agentien und elektroporation für zellstrukturen und verwendung davon

Info

Publication number
DE60217393D1
DE60217393D1 DE60217393T DE60217393T DE60217393D1 DE 60217393 D1 DE60217393 D1 DE 60217393D1 DE 60217393 T DE60217393 T DE 60217393T DE 60217393 T DE60217393 T DE 60217393T DE 60217393 D1 DE60217393 D1 DE 60217393D1
Authority
DE
Germany
Prior art keywords
cell structure
agents
tubes
electroporation
cell structures
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE60217393T
Other languages
English (en)
Other versions
DE60217393T2 (de
Inventor
Owe Orwar
Mattias Karlsson
Kerstin Nolkrantz
Cecilia Farre
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Cellectricon AB
Original Assignee
Cellectricon AB
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Cellectricon AB filed Critical Cellectricon AB
Publication of DE60217393D1 publication Critical patent/DE60217393D1/de
Application granted granted Critical
Publication of DE60217393T2 publication Critical patent/DE60217393T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/87Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61NELECTROTHERAPY; MAGNETOTHERAPY; RADIATION THERAPY; ULTRASOUND THERAPY
    • A61N1/00Electrotherapy; Circuits therefor
    • A61N1/18Applying electric currents by contact electrodes
    • A61N1/20Applying electric currents by contact electrodes continuous direct currents
    • A61N1/30Apparatus for iontophoresis, i.e. transfer of media in ionic state by an electromotoric force into the body, or cataphoresis
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61PSPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P43/00Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/10Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
    • G01N2035/1027General features of the devices
    • G01N2035/1034Transferring microquantities of liquid
    • G01N2035/1039Micropipettes, e.g. microcapillary tubes
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/10Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
    • G01N35/1065Multiple transfer devices
    • G01N35/1074Multiple transfer devices arranged in a two-dimensional array

Landscapes

  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Veterinary Medicine (AREA)
  • Public Health (AREA)
  • Animal Behavior & Ethology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Molecular Biology (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Radiology & Medical Imaging (AREA)
  • Biochemistry (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Medicinal Chemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Crystals, And After-Treatments Of Crystals (AREA)
DE60217393T 2001-11-27 2002-11-27 Verfahren zur kombinierten parallelen zuführung von agentien und elektroporation für zellstrukturen und verwendung davon Expired - Lifetime DE60217393T2 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
SE0103957 2001-11-27
SE0103957 2001-11-27
PCT/SE2002/002192 WO2003046170A1 (en) 2001-11-27 2002-11-27 A method for combined parallel agent delivery and electroporation for cell structures and use thereof

Publications (2)

Publication Number Publication Date
DE60217393D1 true DE60217393D1 (de) 2007-02-15
DE60217393T2 DE60217393T2 (de) 2007-10-18

Family

ID=20286108

Family Applications (1)

Application Number Title Priority Date Filing Date
DE60217393T Expired - Lifetime DE60217393T2 (de) 2001-11-27 2002-11-27 Verfahren zur kombinierten parallelen zuführung von agentien und elektroporation für zellstrukturen und verwendung davon

Country Status (8)

Country Link
US (4) US20050026283A1 (de)
EP (2) EP1448770A1 (de)
JP (2) JP4842512B2 (de)
AT (1) ATE350471T1 (de)
AU (2) AU2002353741A1 (de)
CA (2) CA2468424A1 (de)
DE (1) DE60217393T2 (de)
WO (2) WO2003046170A1 (de)

Families Citing this family (43)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7456012B2 (en) 1997-11-06 2008-11-25 Cellectricon Ab Method and apparatus for spatially confined electroporation
WO2003046170A1 (en) 2001-11-27 2003-06-05 Cellectricon Ab A method for combined parallel agent delivery and electroporation for cell structures and use thereof
CA2475348A1 (en) * 2002-02-12 2003-08-21 Cellectricon Ab Systems and methods for rapidly changing the solution environment around sensors
WO2004036202A1 (en) 2002-10-16 2004-04-29 Cellectricon Ab Nanoelectrodes and nanotips for recording transmembrane currents in a plurality of cells
US8058056B2 (en) 2004-03-12 2011-11-15 The Regents Of The University Of California Method and apparatus for integrated cell handling and measurements
US8293524B2 (en) * 2006-03-31 2012-10-23 Fluxion Biosciences Inc. Methods and apparatus for the manipulation of particle suspensions and testing thereof
WO2009029519A2 (en) * 2007-08-24 2009-03-05 David Mark Evans Multiplexed electroporation apparatus
WO2009067142A1 (en) * 2007-11-21 2009-05-28 The Regents Of The University Of California Controlled multi-holes electroporation
JP5659024B2 (ja) 2008-03-12 2015-01-28 セレクトリコン アーベー マルチウェルプレートにおいてチップを整列させる装置及び方法
ITCO20080016A1 (it) * 2008-04-22 2009-10-23 Giuseppe Caccia Manipolo per elettroporazione di prodotti farmacologici e/o naturali e/o estetici su strati cutanei a spessore contenuto
DE102008045830A1 (de) 2008-09-05 2010-03-11 Cinogy Gmbh Verfahren zur Behandlung eines lebende Zellen enthaltenden biologischen Materials
WO2010064991A1 (en) * 2008-12-01 2010-06-10 Nanyang Polytechnic Electroporation apparatus and method
KR101924394B1 (ko) 2009-11-13 2018-12-03 인튜어티브 서지컬 오퍼레이션즈 인코포레이티드 독립적으로 회전하는 부재 내의 병렬 구동 샤프트들을 위한 모터 연접부
EP2651505A4 (de) 2010-12-15 2014-06-11 Old Dominion Univ Res Found Elektroporationsinduzierte elektrosensibilisierung
USD675171S1 (en) 2010-12-16 2013-01-29 Tecella Llc Multi-electrode holder
CN105132284B (zh) 2011-05-13 2018-04-03 加利福尼亚大学董事会 用于选择性转染细胞的光热衬底
WO2013006343A1 (en) * 2011-07-01 2013-01-10 Beckman Coulter, Inc. Low carryover liquid handling probe for an automated analyzer
JP6035948B2 (ja) * 2011-08-04 2016-11-30 日本精工株式会社 圧電アクチュエータ及びマニピュレータ
CN102335479B (zh) * 2011-09-28 2013-03-13 上海交通大学 植入式微型电渗流可控输药芯片及其制备方法
CN102872531B (zh) * 2011-09-28 2014-11-19 上海交通大学 一种电渗流可控输药芯片电渗驱动单元的制备方法
RU2656156C2 (ru) 2011-10-17 2018-05-31 Массачусетс Инститьют Оф Текнолоджи Внутриклеточная доставка
CN105452471A (zh) 2013-03-15 2016-03-30 加利福尼亚大学董事会 使用光热平台向活细胞中的高通量运输物递送
EP2997126A4 (de) * 2013-05-16 2017-01-18 Advanced Liquid Logic, Inc. Tropfenaktuator zur elektroporation und transformation von zellen
KR102304167B1 (ko) 2013-08-16 2021-09-24 메사추세츠 인스티튜트 오브 테크놀로지 세포로의 선택적 물질 전달
US10472651B2 (en) 2014-03-28 2019-11-12 The Regents Of The University Of California Efficient delivery of large cargos into cells on a porous substrate
WO2016011059A1 (en) * 2014-07-14 2016-01-21 President And Fellows Of Harvard College Drug cocktail analyses using microscale vortex-assisted electroporation
US20170183644A1 (en) * 2014-07-14 2017-06-29 President And Fellows Of Harvard College Drug cocktail analyses using microscale vortex assisted electroporation
PL226157B1 (pl) * 2014-10-21 2017-06-30 Inst Tech Materiałów Elektronicznych Mikrosonda do elektroporacji selektywnej i sposob wytwarzania takiej mikrosondy
RU2739794C2 (ru) 2014-10-31 2020-12-28 Массачусетс Инститьют Оф Текнолоджи Доставка биомолекул в клетки иммунной системы
US10526573B2 (en) 2014-11-14 2020-01-07 Massachusetts Institute Of Technology Disruption and field enabled delivery of compounds and compositions into cells
EP3037517A1 (de) * 2014-12-28 2016-06-29 Femtofab Co., Ltd. Verfahren zur modifizierung einer zelle durch einführung eines materials in die zelle
EP3037518B1 (de) * 2014-12-28 2020-05-06 Femtobiomed Inc. Vorrichtung zum einbringen von material in eine zelle
KR102428464B1 (ko) * 2014-12-28 2022-08-03 주식회사 펨토바이오메드 세포에 물질을 주입하여 세포를 변환시키는 방법
US11125739B2 (en) 2015-01-12 2021-09-21 Massachusetts Institute Of Technology Gene editing through microfluidic delivery
CN104536186B (zh) * 2015-01-13 2017-12-08 合肥鑫晟光电科技有限公司 一种识别装置和对位系统
WO2016161081A1 (en) 2015-04-03 2016-10-06 Fluxion Biosciences, Inc. Molecular characterization of single cells and cell populations for non-invasive diagnostics
WO2016168492A1 (en) 2015-04-14 2016-10-20 Hur Soojung Claire Electrode array for vortex-assisted electroporation
JP6925984B2 (ja) 2015-07-09 2021-08-25 マサチューセッツ インスティテュート オブ テクノロジー 無核細胞への物質の送達
US11884960B2 (en) * 2019-08-16 2024-01-30 Terragene Llc Biological indicator for determining the efficacy of a steam or heat sterilization process and its method of use
EP4025144B1 (de) * 2019-09-02 2023-09-06 Mirai Medical Limited Elektroporationsgerät
KR102247855B1 (ko) 2020-08-06 2021-05-03 김명연 고전압 펄스 전기장을 이용하여 과일 또는 야채 내부에 스테비오사이드 또는 향기성분을 침투시키는 단맛 강화 방법 및 장치
WO2024206004A1 (en) * 2023-03-26 2024-10-03 The Trustees Of Indiana University Paper-based microfluidic platform and method for particle spectrometry analysis
WO2025117977A1 (en) * 2023-11-30 2025-06-05 Met Biotechnology, Inc. Electroporation systems and methods for cargo loading into extracellular vesicles

Family Cites Families (67)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US52A (en) * 1836-10-11 Drawing
US584268A (en) * 1897-06-08 John edward mellor
US3867201A (en) 1971-08-11 1975-02-18 Mallory & Co Inc P R Hermetically sealed electrolyte battery and method of filling without breaking hermetic seal
DE3737607A1 (de) * 1987-11-05 1989-05-24 Hoefelmayr Bio Melktech Verfahren und vorrichtung zur durchfuehrung von messungen an einer schaeumenden fluessigkeit
WO1989006555A1 (en) * 1988-01-21 1989-07-27 Massachusetts Institute Of Technology Transport of molecules across tissue using electroporation
US5389069A (en) * 1988-01-21 1995-02-14 Massachusetts Institute Of Technology Method and apparatus for in vivo electroporation of remote cells and tissue
WO1990004645A1 (en) 1988-10-21 1990-05-03 Molecular Devices Corporation Methods and apparatus for detecting the effect of cell affecting agents on living cells
US5135478A (en) * 1989-05-10 1992-08-04 Drug Delivery Systems Inc. Multi-signal electrical transdermal drug applicator
US5137817A (en) 1990-10-05 1992-08-11 Amoco Corporation Apparatus and method for electroporation
US5208145A (en) * 1991-05-06 1993-05-04 The Salk Institute For Biological Studies Assay for the presence, in cultured cells, of functional ligand-gated channels, and substances which enhance intracellular lithium accumulation by cultured cells via such channels
US5449492A (en) * 1992-01-15 1995-09-12 List Electronics Device for determining concentration-dependent electrophysiological parameters in a series of measurements
US5501662A (en) * 1992-05-22 1996-03-26 Genetronics, Inc. Implantable electroporation method and apparatus for drug and gene delivery
US5597699A (en) * 1992-09-30 1997-01-28 Lanzara; Richard G. Method for determining drug compositions to prevent desensitization of cellular receptors
AU6254894A (en) 1993-03-05 1994-09-26 University Of Wollongong, The Pulsed electrochemical detection using polymer electroactive electrodes
US5702359A (en) * 1995-06-06 1997-12-30 Genetronics, Inc. Needle electrodes for mediated delivery of drugs and genes
US5993434A (en) * 1993-04-01 1999-11-30 Genetronics, Inc. Method of treatment using electroporation mediated delivery of drugs and genes
EP0665430B1 (de) * 1994-01-28 1998-04-15 Hewlett-Packard GmbH Kapillar aus Kunststoff für Kapillarelektroforese und Verfahren zu dessen Herstellung
IL108775A (en) 1994-02-25 2003-09-17 Univ Ramot Method for efficient incorporation of molecules into cells
JPH10511008A (ja) * 1994-06-24 1998-10-27 シグナス, インコーポレイテッド 膜透過性制御のための電子電圧パルスを用いた生物学的活性因子のパルス使用送達システム
WO1996010170A1 (en) 1994-09-29 1996-04-04 The Board Of Trustees Of The Leland Stanford Junior University Capillary-based separation methods for identifying bioactive analytes in a mixture
DE19529371C3 (de) 1995-08-10 2003-05-28 Nmi Univ Tuebingen Mikroelektroden-Anordnung
US6283951B1 (en) * 1996-10-11 2001-09-04 Transvascular, Inc. Systems and methods for delivering drugs to selected locations within the body
US6762036B2 (en) 1995-11-08 2004-07-13 Trustees Of Boston University Cellular physiology workstations for automated data acquisition and perfusion control
US5874268A (en) * 1996-09-23 1999-02-23 Duke University Method of introducing exogenous compounds into cells by electroporation and apparatus for same
US6352535B1 (en) * 1997-09-25 2002-03-05 Nanoptics, Inc. Method and a device for electro microsurgery in a physiological liquid environment
US5885430A (en) * 1996-10-04 1999-03-23 Spectrumedix Corporation Capillary tube holder for an electrophoretic apparatus
WO1998022819A1 (de) * 1996-11-16 1998-05-28 Nmi Naturwissenschaftliches Und Medizinisches Institut An Der Universität Tübingen In Reutlingen Stiftung Bürgerlichen Rechts Mikroelementenanordnung, verfahren zum kontaktieren von in einer flüssigen umgebung befindlichen zellen und verfahren zum herstellen einer mikroelementenanordnung
US5873849A (en) * 1997-04-24 1999-02-23 Ichor Medical Systems, Inc. Electrodes and electrode arrays for generating electroporation inducing electrical fields
EP0980523B1 (de) * 1997-05-01 2006-12-27 Sophion Bioscience A/S Vorrichtung zur automatischen elektrodenpositionierung
SE9702112D0 (sv) 1997-06-04 1997-06-04 Holdingbolaget Vid Goeteborgs Method and apparatus for detection of a receptor antagonist
US6241666B1 (en) 1997-07-03 2001-06-05 Cardiac Pathways Corp. Ablation catheter tip with a buffer layer covering the electrode
US6055453A (en) * 1997-08-01 2000-04-25 Genetronics, Inc. Apparatus for addressing needle array electrodes for electroporation therapy
US6241701B1 (en) * 1997-08-01 2001-06-05 Genetronics, Inc. Apparatus for electroporation mediated delivery of drugs and genes
WO2003046170A1 (en) 2001-11-27 2003-06-05 Cellectricon Ab A method for combined parallel agent delivery and electroporation for cell structures and use thereof
US7456012B2 (en) * 1997-11-06 2008-11-25 Cellectricon Ab Method and apparatus for spatially confined electroporation
SE9704076D0 (sv) * 1997-11-06 1997-11-06 Holdingbolaget Vid Goeteborgs Method for permeabilisation of cell structures and use thereof
US6208893B1 (en) 1998-01-27 2001-03-27 Genetronics, Inc. Electroporation apparatus with connective electrode template
AU2150300A (en) * 1998-12-07 2000-06-26 Acacia Biosciences Inc. Multi-channel electrode arrays
US6713291B2 (en) * 1999-01-28 2004-03-30 Alan D. King Electrodes coated with treating agent and uses thereof
US6193647B1 (en) * 1999-04-08 2001-02-27 The Board Of Trustees Of The University Of Illinois Microfluidic embryo and/or oocyte handling device and method
US6300108B1 (en) 1999-07-21 2001-10-09 The Regents Of The University Of California Controlled electroporation and mass transfer across cell membranes
SE9902817D0 (sv) 1999-07-30 1999-07-30 A & Science Invest Ab A method for selective electrofusion of at least two fusion partners having cell-like membranes
US6326177B1 (en) 1999-08-04 2001-12-04 Eastern Virginia Medical School Of The Medical College Of Hampton Roads Method and apparatus for intracellular electro-manipulation
ATE283092T1 (de) 1999-12-15 2004-12-15 Univ South Florida Vorrichtung zur elektroporation
CA2395492A1 (en) 1999-12-22 2001-07-05 Diana Wang Method and apparatus for targeting localised electroporation
DE10010081A1 (de) * 2000-03-02 2001-09-13 Nmi Univ Tuebingen Vorrichtung und Elektrodenanordnung für elektrophysiologische Untersuchungen
US7011939B2 (en) * 2000-03-22 2006-03-14 Abbott Laboratories Apparatus and method for electrophysiological testing
GB0013584D0 (en) 2000-06-06 2000-07-26 Cenes Ltd Automated flow patch-clamp system
DE10032568A1 (de) * 2000-07-05 2002-01-24 Nmi Univ Tuebingen Vorrichtung und Verfahren zum elektrischen Kontaktieren von in einer Flüssigkeit in Suspension befindlichen biologischen Zellen
EP1178315A1 (de) * 2000-07-31 2002-02-06 Albrecht Dr.med. Priv.Doz. Lepple-Wienhues Verfahren und Vorrichtung zur Untersuchung von Zellen mit Hilfe der Patch Clamp-Methode
WO2002022787A2 (en) * 2000-09-18 2002-03-21 President And Fellows Of Harvard College Differential treatment of selected parts of a single cell with different fluid components
US6932893B2 (en) * 2000-10-02 2005-08-23 Sophion Bioscience A/S System for electrophysiological measurements
US6846306B1 (en) 2000-10-10 2005-01-25 Cold Spring Harbor Laboratory Single cell electroporation
SE0003841D0 (sv) 2000-10-20 2000-10-20 Daniel Chiu A method and apparatus for penetration of lipid bilayer membranes
EP1225216A1 (de) * 2001-01-08 2002-07-24 Niels Fertig Vorrichtung zur Untersuchung von Ionenkanälen in Membranen
US7101703B2 (en) * 2001-03-09 2006-09-05 Cornell Research Foundation, Inc. Electrofusion microelectrode
CA2441366A1 (en) * 2001-03-24 2002-10-03 Aviva Biosciences Corporation Biochips including ion transport detecting structures and methods of use
US6748266B2 (en) 2001-04-06 2004-06-08 Mattioli Engineering Ltd. Method and apparatus for skin absorption enhancement and transdermal drug delivery
US7127284B2 (en) * 2001-06-11 2006-10-24 Mercator Medsystems, Inc. Electroporation microneedle and methods for its use
US6749792B2 (en) * 2001-07-09 2004-06-15 Lifescan, Inc. Micro-needles and methods of manufacture and use thereof
US7018819B2 (en) 2001-11-30 2006-03-28 Cellectricon Ab Method and apparatus for manipulation of cells and cell-like structures focused electric fields in microfludic systems and use thereof
US7470518B2 (en) * 2002-02-12 2008-12-30 Cellectricon Ab Systems and method for rapidly changing the solution environment around sensors
CA2475348A1 (en) 2002-02-12 2003-08-21 Cellectricon Ab Systems and methods for rapidly changing the solution environment around sensors
WO2004018690A1 (en) 2002-08-21 2004-03-04 Cellectricon Ab System and method for obtaining and maintaining high resistance seals in patch clamp recordings
AU2003279926A1 (en) 2002-10-09 2004-05-04 Cellectricon Ab Method for interfacing macroscale components to microscale devices
WO2004036202A1 (en) 2002-10-16 2004-04-29 Cellectricon Ab Nanoelectrodes and nanotips for recording transmembrane currents in a plurality of cells
AU2003283663A1 (en) 2002-11-01 2004-05-25 Cellectricon Ab Computer programs,workstations, systems and methods for microfluidic substrates in cell

Also Published As

Publication number Publication date
US8268555B2 (en) 2012-09-18
WO2003046170A1 (en) 2003-06-05
ATE350471T1 (de) 2007-01-15
AU2002353741A1 (en) 2003-06-10
AU2002365542A1 (en) 2003-06-10
US20050026283A1 (en) 2005-02-03
CA2468424A1 (en) 2003-06-05
EP1448770A1 (de) 2004-08-25
JP2005510237A (ja) 2005-04-21
EP1448771B1 (de) 2007-01-03
JP2005510236A (ja) 2005-04-21
EP1448771A1 (de) 2004-08-25
JP4842512B2 (ja) 2011-12-21
DE60217393T2 (de) 2007-10-18
CA2468511A1 (en) 2003-06-05
US20050019921A1 (en) 2005-01-27
US20100159439A1 (en) 2010-06-24
US20090092963A1 (en) 2009-04-09
US8338150B2 (en) 2012-12-25
WO2003046171A1 (en) 2003-06-05

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