DK0378382T3 - Rekombinant DNA-fremgangsmåde og værtsceller - Google Patents

Rekombinant DNA-fremgangsmåde og værtsceller

Info

Publication number
DK0378382T3
DK0378382T3 DK90300270.7T DK90300270T DK0378382T3 DK 0378382 T3 DK0378382 T3 DK 0378382T3 DK 90300270 T DK90300270 T DK 90300270T DK 0378382 T3 DK0378382 T3 DK 0378382T3
Authority
DK
Denmark
Prior art keywords
host cells
recombinant dna
dna method
amount
transcription unit
Prior art date
Application number
DK90300270.7T
Other languages
English (en)
Inventor
Mark Ian Cockett
Christopher Robert Bebbington
Geoffrey Thomas Yarranton
Original Assignee
Celltech Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Celltech Ltd filed Critical Celltech Ltd
Application granted granted Critical
Publication of DK0378382T3 publication Critical patent/DK0378382T3/da

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2800/00—Nucleic acids vectors
    • C12N2800/10—Plasmid DNA
    • C12N2800/108—Plasmid DNA episomal vectors

Landscapes

  • Genetics & Genomics (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Multi Processors (AREA)
  • Computer And Data Communications (AREA)
  • Communication Control (AREA)
  • Saccharide Compounds (AREA)
DK90300270.7T 1989-01-10 1990-01-10 Rekombinant DNA-fremgangsmåde og værtsceller DK0378382T3 (da)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
GB898900483A GB8900483D0 (en) 1989-01-10 1989-01-10 Recombinant dna method

Publications (1)

Publication Number Publication Date
DK0378382T3 true DK0378382T3 (da) 1997-06-16

Family

ID=10649831

Family Applications (1)

Application Number Title Priority Date Filing Date
DK90300270.7T DK0378382T3 (da) 1989-01-10 1990-01-10 Rekombinant DNA-fremgangsmåde og værtsceller

Country Status (12)

Country Link
US (2) US5866359A (da)
EP (1) EP0378382B1 (da)
JP (1) JP3070950B2 (da)
AT (1) ATE153382T1 (da)
AU (2) AU4825390A (da)
CA (1) CA2024607C (da)
DE (1) DE69030740T2 (da)
DK (1) DK0378382T3 (da)
ES (1) ES2103266T3 (da)
GB (1) GB8900483D0 (da)
GR (1) GR3024002T3 (da)
WO (1) WO1990008191A1 (da)

Families Citing this family (25)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB8900483D0 (en) 1989-01-10 1989-03-08 Celltech Ltd Recombinant dna method
EP0455424A3 (en) * 1990-05-02 1992-04-29 Merck & Co. Inc. Mammalian inducible promoter cascade system
CA2047633A1 (en) * 1990-09-12 1992-03-13 Ben Bowen Inactivation of gene transcription in plants using altered transcriptional activators
JP2844993B2 (ja) 1991-11-13 1999-01-13 矢崎総業株式会社 ジョイント端子の防水接続方法
DE69328514T2 (de) 1992-03-13 2001-01-11 Monsanto Co., St. Louis Herstellung rekombinanter Proteine unter Verwendung von Herpes-Virus-Promotoren und VP16-Transaktivatoren
US5658763A (en) * 1993-10-25 1997-08-19 Creative Biomolecules, Inc. Methods and compositions for high protein production from non-native DNA
US5585237A (en) * 1993-10-25 1996-12-17 Creative Biomolecules, Inc. Methods and compositions for high protein production from recombinant DNA
KR100470180B1 (ko) 1995-06-15 2005-02-04 크루셀 홀란드 비.브이. 유전자 요법에 사용할 수 있는 인간의 재조합아데노바이러스 패키징 시스템
US20050164386A1 (en) * 1999-04-15 2005-07-28 Uytdehaag Alphonsus G. Overexpression of enzymes involved in post-translational protein modifications in human cells
US7297680B2 (en) * 1999-04-15 2007-11-20 Crucell Holland B.V. Compositions of erythropoietin isoforms comprising Lewis-X structures and high sialic acid content
US8236561B2 (en) * 1999-04-15 2012-08-07 Crucell Holland B.V. Efficient production of IgA in recombinant mammalian cells
US7604960B2 (en) * 1999-04-15 2009-10-20 Crucell Holland B.V. Transient protein expression methods
US20050170463A1 (en) * 1999-04-15 2005-08-04 Abraham Bout Recombinant protein production in permanent amniocytic cells that comprise nucleic acid encoding adenovirus E1A and E1B proteins
CN100457914C (zh) * 1999-04-15 2009-02-04 荷兰克鲁塞尔公司 用编码腺病毒e1蛋白的序列在人体细胞中生产重组蛋白
US6855544B1 (en) 1999-04-15 2005-02-15 Crucell Holland B.V. Recombinant protein production in a human cell
US7192759B1 (en) * 1999-11-26 2007-03-20 Crucell Holland B.V. Production of vaccines
US7527961B2 (en) * 1999-11-26 2009-05-05 Crucell Holland B.V. Production of vaccines
US7521220B2 (en) * 1999-11-26 2009-04-21 Crucell Holland B.V. Production of vaccines
CA2441937A1 (en) * 2001-05-30 2002-12-05 Chromos Molecular Systems, Inc. Chromosome-based platforms
JP4480398B2 (ja) * 2001-12-07 2010-06-16 クルセル ホランド ベー ヴェー ウイルス、ウイルス分離株及びワクチンの製造方法
KR101183770B1 (ko) 2003-05-09 2012-09-17 크루셀 홀란드 비.브이. Ｅ1-불멸화된 세포의 배양물 및 이로부터 생성 수율을 증가시키기 위한 동일물의 배양 방법
DE602004027483D1 (de) * 2003-05-23 2010-07-15 Crucell Holland Bv Herstellung von rekombinanten igm in den per.c6 zellen
EP1702071B1 (en) 2003-12-31 2012-02-22 Kalobios Inc. Transactivation system for mammalian cells
EP2711426B1 (en) 2012-09-24 2015-04-08 Lonza Biologics plc Expression vectors comprising chimeric cytomegalovirus promoter and enhancer sequences
EP2829608A1 (en) 2013-07-23 2015-01-28 Universität Bielefeld Method for recombinant protein production in mammalian cells

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4740461A (en) * 1983-12-27 1988-04-26 Genetics Institute, Inc. Vectors and methods for transformation of eucaryotic cells
EP0245949B1 (en) * 1986-04-09 1997-10-29 Eli Lilly And Company A method of using eukaryotic expression vectors comprising the bk virus enhancer
US4775630A (en) * 1986-08-15 1988-10-04 Vanderbilt University Transcriptional control element adapted for regulation of gene expression in animal cells
EP0349598A1 (en) * 1987-03-13 1990-01-10 Schering Corporation Method for stimulating gene expression
US5024939A (en) * 1987-07-09 1991-06-18 Genentech, Inc. Transient expression system for producing recombinant protein
GB8900483D0 (en) 1989-01-10 1989-03-08 Celltech Ltd Recombinant dna method

Also Published As

Publication number Publication date
GR3024002T3 (en) 1997-10-31
JP3070950B2 (ja) 2000-07-31
US5866359A (en) 1999-02-02
ES2103266T3 (es) 1997-09-16
CA2024607A1 (en) 1990-07-11
US6653101B1 (en) 2003-11-25
AU3994193A (en) 1993-08-12
ATE153382T1 (de) 1997-06-15
JPH03503959A (ja) 1991-09-05
DE69030740T2 (de) 1997-11-27
CA2024607C (en) 1999-11-30
EP0378382A1 (en) 1990-07-18
DE69030740D1 (de) 1997-06-26
GB8900483D0 (en) 1989-03-08
WO1990008191A1 (en) 1990-07-26
AU662416B2 (en) 1995-08-31
AU4825390A (en) 1990-08-13
EP0378382B1 (en) 1997-05-21

Similar Documents

Publication Publication Date Title
DE69030740D1 (de) Rekombinantes DNS-Verfahren und Wirtszellen
DK97784A (da) Dna-segment
AU7945682A (en) Rennin from recombinant dna
AU2872089A (en) Receptor protein for human b cell stimulatory factor-2
DK163187C (da) Fremgangsmaader til fremstilling af humant immuninterferon (gamma-interferon) samt dna-fragmenter, rekombinante klonings- og udtrykkelsesvektorer og rekombinante mikroorganismer og cellekulturer til anvendelse herved
DK506488D0 (da) Fremgangsmaade til dyrkning af rekombinante celler, vaertscelle til anvendelse ved fremgangsmaaden og kultur omfattende vaertscellen
AU8110487A (en) DNA molecules coding for proteins targeted to plant cell walls
DK0547064T3 (da) Proteaseresistent PDGF og anvendelsesfremgangsmåde
PT83968A (de) Verfahren zur verbesserung des expression von eukaryontenprotein
ES2078243T3 (es) Mejoras en o relativas a la expresion eucariotica.
GB2171703B (en) Expression-secretion vector for gene expression and protein secretion, recombinant dna including the vector, and method of producing proteins by use of the re
IL87394A0 (en) Expression vectors for the synthesis of proteins and plasmid replicons and sequence cassettes for use in constructing said vectors
SE8605459L (sv) Ny celltillvextreglerande faktor
GB2183656B (en) A method for the expression of genes in yeast, and dna fragments as well as plasmids comprising said dna fragments to be used when carrying out the method
DE58909205D1 (de) Expressionsvektor und Verfahren zur regulierbaren Herstellung von Proteinen in Eukaryonten.