ATE153382T1 - Rekombinantes dns-verfahren und wirtszellen - Google Patents

Rekombinantes dns-verfahren und wirtszellen

Info

Publication number
ATE153382T1
ATE153382T1 AT90300270T AT90300270T ATE153382T1 AT E153382 T1 ATE153382 T1 AT E153382T1 AT 90300270 T AT90300270 T AT 90300270T AT 90300270 T AT90300270 T AT 90300270T AT E153382 T1 ATE153382 T1 AT E153382T1
Authority
AT
Austria
Prior art keywords
host cells
recombinant dna
dna process
amount
transcription unit
Prior art date
Application number
AT90300270T
Other languages
English (en)
Inventor
Mark Ian Cockett
Christopher Robert Bebbington
Geoffrey Thomas Yarranton
Original Assignee
Celltech Therapeutics Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Celltech Therapeutics Ltd filed Critical Celltech Therapeutics Ltd
Application granted granted Critical
Publication of ATE153382T1 publication Critical patent/ATE153382T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2800/00—Nucleic acids vectors
    • C12N2800/10—Plasmid DNA
    • C12N2800/108—Plasmid DNA episomal vectors

Landscapes

  • Genetics & Genomics (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Communication Control (AREA)
  • Multi Processors (AREA)
  • Computer And Data Communications (AREA)
  • Saccharide Compounds (AREA)
AT90300270T 1989-01-10 1990-01-10 Rekombinantes dns-verfahren und wirtszellen ATE153382T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
GB898900483A GB8900483D0 (en) 1989-01-10 1989-01-10 Recombinant dna method

Publications (1)

Publication Number Publication Date
ATE153382T1 true ATE153382T1 (de) 1997-06-15

Family

ID=10649831

Family Applications (1)

Application Number Title Priority Date Filing Date
AT90300270T ATE153382T1 (de) 1989-01-10 1990-01-10 Rekombinantes dns-verfahren und wirtszellen

Country Status (12)

Country Link
US (2) US5866359A (de)
EP (1) EP0378382B1 (de)
JP (1) JP3070950B2 (de)
AT (1) ATE153382T1 (de)
AU (2) AU4825390A (de)
CA (1) CA2024607C (de)
DE (1) DE69030740T2 (de)
DK (1) DK0378382T3 (de)
ES (1) ES2103266T3 (de)
GB (1) GB8900483D0 (de)
GR (1) GR3024002T3 (de)
WO (1) WO1990008191A1 (de)

Families Citing this family (25)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB8900483D0 (en) 1989-01-10 1989-03-08 Celltech Ltd Recombinant dna method
EP0455424A3 (en) * 1990-05-02 1992-04-29 Merck & Co. Inc. Mammalian inducible promoter cascade system
CA2047633A1 (en) * 1990-09-12 1992-03-13 Ben Bowen Inactivation of gene transcription in plants using altered transcriptional activators
JP2844993B2 (ja) 1991-11-13 1999-01-13 矢崎総業株式会社 ジョイント端子の防水接続方法
CA2092645A1 (en) 1992-03-13 1993-09-14 Maureen K. Highkin Production of recombinant proteins using herpes virus promoters and vp16 transactivators
US5585237A (en) * 1993-10-25 1996-12-17 Creative Biomolecules, Inc. Methods and compositions for high protein production from recombinant DNA
US5658763A (en) * 1993-10-25 1997-08-19 Creative Biomolecules, Inc. Methods and compositions for high protein production from non-native DNA
DE69633565T3 (de) 1995-06-15 2013-01-17 Crucell Holland B.V. Verpackungssysteme für humane, menschliche adenoviren, zur verwendung in die gentherapie
US7604960B2 (en) * 1999-04-15 2009-10-20 Crucell Holland B.V. Transient protein expression methods
US20050170463A1 (en) * 1999-04-15 2005-08-04 Abraham Bout Recombinant protein production in permanent amniocytic cells that comprise nucleic acid encoding adenovirus E1A and E1B proteins
US20050164386A1 (en) * 1999-04-15 2005-07-28 Uytdehaag Alphonsus G. Overexpression of enzymes involved in post-translational protein modifications in human cells
US8236561B2 (en) * 1999-04-15 2012-08-07 Crucell Holland B.V. Efficient production of IgA in recombinant mammalian cells
US6855544B1 (en) 1999-04-15 2005-02-15 Crucell Holland B.V. Recombinant protein production in a human cell
US7297680B2 (en) * 1999-04-15 2007-11-20 Crucell Holland B.V. Compositions of erythropoietin isoforms comprising Lewis-X structures and high sialic acid content
ES2378272T3 (es) * 1999-04-15 2012-04-10 Crucell Holland B.V. Producción de proteína recombinante en una célula humana
US7527961B2 (en) * 1999-11-26 2009-05-05 Crucell Holland B.V. Production of vaccines
US7192759B1 (en) * 1999-11-26 2007-03-20 Crucell Holland B.V. Production of vaccines
US7521220B2 (en) * 1999-11-26 2009-04-21 Crucell Holland B.V. Production of vaccines
JP4489424B2 (ja) * 2001-05-30 2010-06-23 グラクソ グループ リミテッド 染色体に基くプラットホーム
ATE384785T1 (de) * 2001-12-07 2008-02-15 Crucell Holland Bv Herstellung von viren, virusisolaten, und impfstoffen
NZ542585A (en) * 2003-05-09 2007-11-30 Crucell Holland Bv Cultures of PER.C6-immortalized cells and processes for culturing the same to increase product yields therefrom
DK1626992T3 (da) * 2003-05-23 2010-09-20 Crucell Holland Bv Produktion af rekombinant IgM i PER.C6-celler
EP1702071B1 (de) 2003-12-31 2012-02-22 Kalobios Inc. Transaktivierungssystem für säugerzellen
HUE026516T2 (en) 2012-09-24 2016-06-28 Lonza Biologics Plc Expression vectors containing chimeric cytomegalovirus promoter and enhancer sequences
EP2829608A1 (de) 2013-07-23 2015-01-28 Universität Bielefeld Verfahren zur Herstellung von rekombinanten Proteinen in Säugerzellen

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4740461A (en) * 1983-12-27 1988-04-26 Genetics Institute, Inc. Vectors and methods for transformation of eucaryotic cells
EP0245949B1 (de) * 1986-04-09 1997-10-29 Eli Lilly And Company Verfahren zur Anwendung von den BK-Virus-Verstärker enthaltenden eukaryotischen Expressionsvektoren
US4775630A (en) * 1986-08-15 1988-10-04 Vanderbilt University Transcriptional control element adapted for regulation of gene expression in animal cells
EP0282330A3 (de) * 1987-03-13 1988-11-09 Schering Corporation Verfahren zur Stimulierung der Genexpression
US5024939A (en) * 1987-07-09 1991-06-18 Genentech, Inc. Transient expression system for producing recombinant protein
GB8900483D0 (en) 1989-01-10 1989-03-08 Celltech Ltd Recombinant dna method

Also Published As

Publication number Publication date
GB8900483D0 (en) 1989-03-08
CA2024607A1 (en) 1990-07-11
WO1990008191A1 (en) 1990-07-26
US5866359A (en) 1999-02-02
JP3070950B2 (ja) 2000-07-31
DE69030740T2 (de) 1997-11-27
GR3024002T3 (en) 1997-10-31
JPH03503959A (ja) 1991-09-05
AU3994193A (en) 1993-08-12
AU662416B2 (en) 1995-08-31
AU4825390A (en) 1990-08-13
EP0378382A1 (de) 1990-07-18
EP0378382B1 (de) 1997-05-21
US6653101B1 (en) 2003-11-25
DK0378382T3 (da) 1997-06-16
ES2103266T3 (es) 1997-09-16
CA2024607C (en) 1999-11-30
DE69030740D1 (de) 1997-06-26

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Legal Events

Date Code Title Description
EEIH Change in the person of patent owner
EELA Cancelled due to lapse of time