DK0410228T3 - Termoinducerbar udtrykkelse af heterologe gener i Bacillus subtilis og midler og fremgangsmåder til opnåelse heraf - Google Patents

Termoinducerbar udtrykkelse af heterologe gener i Bacillus subtilis og midler og fremgangsmåder til opnåelse heraf

Info

Publication number
DK0410228T3
DK0410228T3 DK90113416.3T DK90113416T DK0410228T3 DK 0410228 T3 DK0410228 T3 DK 0410228T3 DK 90113416 T DK90113416 T DK 90113416T DK 0410228 T3 DK0410228 T3 DK 0410228T3
Authority
DK
Denmark
Prior art keywords
thermo
obtaining
methods
bacillus subtilis
inducible expression
Prior art date
Application number
DK90113416.3T
Other languages
English (en)
Inventor
Bellini Ada Velati
Paola Cosmina
Ferra Francesca De
Guido Grandi
Original Assignee
Eniricerche Spa
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Eniricerche Spa filed Critical Eniricerche Spa
Application granted granted Critical
Publication of DK0410228T3 publication Critical patent/DK0410228T3/da

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/75—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Bacillus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C12N15/73—Expression systems using phage (lambda) regulatory sequences

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Virology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Indole Compounds (AREA)
DK90113416.3T 1989-07-27 1990-07-13 Termoinducerbar udtrykkelse af heterologe gener i Bacillus subtilis og midler og fremgangsmåder til opnåelse heraf DK0410228T3 (da)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
IT8921343A IT1231016B (it) 1989-07-27 1989-07-27 Espressione termoinducibile di geni eterologhi in bacillus subtilis e mezzi e metodi per il suo ottenimento

Publications (1)

Publication Number Publication Date
DK0410228T3 true DK0410228T3 (da) 1994-10-24

Family

ID=11180394

Family Applications (1)

Application Number Title Priority Date Filing Date
DK90113416.3T DK0410228T3 (da) 1989-07-27 1990-07-13 Termoinducerbar udtrykkelse af heterologe gener i Bacillus subtilis og midler og fremgangsmåder til opnåelse heraf

Country Status (8)

Country Link
EP (1) EP0410228B1 (da)
JP (1) JPH03151882A (da)
AT (1) ATE107359T1 (da)
CA (1) CA2022135A1 (da)
DE (1) DE69009883T2 (da)
DK (1) DK0410228T3 (da)
ES (1) ES2055236T3 (da)
IT (1) IT1231016B (da)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6617148B2 (en) * 2000-06-29 2003-09-09 E. I. Du Pont De Nemours And Company Natural promoters for gene expression and metabolic monitoring in bacillus species
WO2006002574A1 (fr) * 2004-07-05 2006-01-12 Nanjing Normal University Vecteur de plasmide regule par le facteur sigma 32 de escherichia coli et son utilisation dans l'expression d'une proteine heterologue
CN114672502B (zh) * 2022-01-06 2024-07-30 清华大学 温控无细胞反应体系的构建方法和该方法使用的质粒以及用途
CN116064522A (zh) * 2022-08-08 2023-05-05 南京工业大学 解淀粉芽孢杆菌来源的温度诱导型启动子及其应用

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB2166743B (en) * 1984-11-13 1989-01-05 Solvay Process for a thermo-inducible gene expression in gram positive bacteria and bacillus subtilis strains containing plasmids which induce such an expression
DE3619902A1 (de) * 1986-06-13 1988-03-03 Biotechnolog Forschung Gmbh Cmv-dna-strukturen, expressionsvektor dafuer, cmv-proteinstrukturen und deren verwendung

Also Published As

Publication number Publication date
IT8921343A0 (it) 1989-07-27
ATE107359T1 (de) 1994-07-15
DE69009883T2 (de) 1994-09-22
IT1231016B (it) 1991-11-08
JPH03151882A (ja) 1991-06-28
EP0410228B1 (en) 1994-06-15
EP0410228A1 (en) 1991-01-30
CA2022135A1 (en) 1991-01-28
ES2055236T3 (es) 1994-08-16
DE69009883D1 (de) 1994-07-21

Similar Documents

Publication Publication Date Title
ES2127458T3 (es) Produccion de proteinas utilizando recombinacion homologa.
DE3854249D1 (de) Rekombinante Humicola-Lipase und Verfahren zur Herstellung von rekombinanten Humicola-Lipasen.
FI871144A0 (fi) Foerfarande foer framstaellning av proteinprodukter i aspegillus oryzae och en promotor foer anvaendning i aspergillus.
AU3679389A (en) Regulatory region of gene which is turned on at late stage in microbial growth cycle
GB8718779D0 (en) Dna sequence & expression vector
FI841976A7 (fi) Plasmider med villkorligt okontrollerat replikeringsuppfoerande.
DK0410228T3 (da) Termoinducerbar udtrykkelse af heterologe gener i Bacillus subtilis og midler og fremgangsmåder til opnåelse heraf
ATE47882T1 (de) Plasmidvektoren zur expression in escherichia coli und/oder bacillus subtilis.
JO1694B1 (en) New chemical products
ATE391182T1 (de) Methode zur kontrolle genetischer expressionen
GR3017101T3 (en) Improvements in or relating to eukaryotic expression.
FI824238L (fi) Foerfarande och vektororganism foer ackumulering av klonade heterologa genprodukter reglerat i bacillus subtilis ii
DK0975775T3 (da) Regulatorisk system til inducerbar ekspression af gener med lambdapromotorer
ES2025383A6 (es) Procemiento para la produccion de hormona de crecimiento porcina
DE3889562D1 (de) Rekombinante Plasmide, die zur Expression von heterologen Proteinen in Bacillus verwendet werden können.
DE58909205D1 (de) Expressionsvektor und Verfahren zur regulierbaren Herstellung von Proteinen in Eukaryonten.
DK476088A (da) Rekombinant humicola lipase og fremgangsmaade til fremstilling deraf