ES2055236T3 - La expresion termo-inducible de genes heterologos en bacillus subtilis y medios y metodos para su consecucion. - Google Patents

La expresion termo-inducible de genes heterologos en bacillus subtilis y medios y metodos para su consecucion.

Info

Publication number
ES2055236T3
ES2055236T3 ES90113416T ES90113416T ES2055236T3 ES 2055236 T3 ES2055236 T3 ES 2055236T3 ES 90113416 T ES90113416 T ES 90113416T ES 90113416 T ES90113416 T ES 90113416T ES 2055236 T3 ES2055236 T3 ES 2055236T3
Authority
ES
Spain
Prior art keywords
expression
heterological
inductible
achievement
thermo
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES90113416T
Other languages
English (en)
Inventor
Paola Cosmina
Bellini Ada Velati
Ferra Francesca De
Guido Grandi
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Eni Tecnologie SpA
Original Assignee
Eniricerche SpA
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Eniricerche SpA filed Critical Eniricerche SpA
Application granted granted Critical
Publication of ES2055236T3 publication Critical patent/ES2055236T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/75—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Bacillus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C12N15/73—Expression systems using phage (lambda) regulatory sequences

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Virology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Indole Compounds (AREA)

Abstract

LA REGULACION DE LA EXPRESION DEL GENE HETEROLOGO QUE CODIFICA UNA PROTEINA DE INTERES EN EL BACILO "SUBTILIS" SE LLEVA A CABO MEDIANTE LA INTRODUCCION EN EL INTERIOR DEL MICROORGANISMO DE UN VECTOR PLASMIDO QUE CONTENGA EL GENE SITUADO BAJO EL CONTROL DE UN FUERTE PROMOTOR CUYA ACTIVIDAD SE MODULA POR MEDIO DE UNA VARIACION EN LA TEMPERATURA MEDIANTE EL REPRESOR TERMOSENSIBLE DE LA FASE LAMBDA DEL "E.COLI". EL METODO POSIBILITA QUE VARIAS PROTEINAS HETEROLOGAS SEAN SINTETIZADAS MEDIANTE EL CULTIVO DE RAMAS DEL BACILO "SUBTILIS" CON TRANSFORMACIONES INDUCIDAS MEDIANTE VARIACIONES EN LA TEMPERATURA.
ES90113416T 1989-07-27 1990-07-13 La expresion termo-inducible de genes heterologos en bacillus subtilis y medios y metodos para su consecucion. Expired - Lifetime ES2055236T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
IT8921343A IT1231016B (it) 1989-07-27 1989-07-27 Espressione termoinducibile di geni eterologhi in bacillus subtilis e mezzi e metodi per il suo ottenimento

Publications (1)

Publication Number Publication Date
ES2055236T3 true ES2055236T3 (es) 1994-08-16

Family

ID=11180394

Family Applications (1)

Application Number Title Priority Date Filing Date
ES90113416T Expired - Lifetime ES2055236T3 (es) 1989-07-27 1990-07-13 La expresion termo-inducible de genes heterologos en bacillus subtilis y medios y metodos para su consecucion.

Country Status (8)

Country Link
EP (1) EP0410228B1 (es)
JP (1) JPH03151882A (es)
AT (1) ATE107359T1 (es)
CA (1) CA2022135A1 (es)
DE (1) DE69009883T2 (es)
DK (1) DK0410228T3 (es)
ES (1) ES2055236T3 (es)
IT (1) IT1231016B (es)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6617148B2 (en) * 2000-06-29 2003-09-09 E. I. Du Pont De Nemours And Company Natural promoters for gene expression and metabolic monitoring in bacillus species
WO2006002574A1 (fr) * 2004-07-05 2006-01-12 Nanjing Normal University Vecteur de plasmide regule par le facteur sigma 32 de escherichia coli et son utilisation dans l'expression d'une proteine heterologue
CN114672502B (zh) * 2022-01-06 2024-07-30 清华大学 温控无细胞反应体系的构建方法和该方法使用的质粒以及用途
CN116064522A (zh) * 2022-08-08 2023-05-05 南京工业大学 解淀粉芽孢杆菌来源的温度诱导型启动子及其应用

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB2166743B (en) * 1984-11-13 1989-01-05 Solvay Process for a thermo-inducible gene expression in gram positive bacteria and bacillus subtilis strains containing plasmids which induce such an expression
DE3619902A1 (de) * 1986-06-13 1988-03-03 Biotechnolog Forschung Gmbh Cmv-dna-strukturen, expressionsvektor dafuer, cmv-proteinstrukturen und deren verwendung

Also Published As

Publication number Publication date
DK0410228T3 (da) 1994-10-24
IT8921343A0 (it) 1989-07-27
ATE107359T1 (de) 1994-07-15
DE69009883T2 (de) 1994-09-22
IT1231016B (it) 1991-11-08
JPH03151882A (ja) 1991-06-28
EP0410228B1 (en) 1994-06-15
EP0410228A1 (en) 1991-01-30
CA2022135A1 (en) 1991-01-28
DE69009883D1 (de) 1994-07-21

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