DK0456782T3 - TMB formulering til opløselig og præcipiterbar HRP-ELISA - Google Patents
TMB formulering til opløselig og præcipiterbar HRP-ELISAInfo
- Publication number
- DK0456782T3 DK0456782T3 DK90916953.4T DK90916953T DK0456782T3 DK 0456782 T3 DK0456782 T3 DK 0456782T3 DK 90916953 T DK90916953 T DK 90916953T DK 0456782 T3 DK0456782 T3 DK 0456782T3
- Authority
- DK
- Denmark
- Prior art keywords
- tmb
- hrp
- solvent
- precipitable
- elisa
- Prior art date
Links
- 238000002965 ELISA Methods 0.000 title abstract 2
- 238000009472 formulation Methods 0.000 title 1
- 239000000203 mixture Substances 0.000 title 1
- 239000002904 solvent Substances 0.000 abstract 4
- WHNWPMSKXPGLAX-UHFFFAOYSA-N N-Vinyl-2-pyrrolidone Chemical compound C=CN1CCCC1=O WHNWPMSKXPGLAX-UHFFFAOYSA-N 0.000 abstract 3
- 235000010443 alginic acid Nutrition 0.000 abstract 2
- 239000000783 alginic acid Substances 0.000 abstract 2
- 229920000615 alginic acid Polymers 0.000 abstract 2
- 229960001126 alginic acid Drugs 0.000 abstract 2
- 150000004781 alginic acids Chemical class 0.000 abstract 2
- 239000012062 aqueous buffer Substances 0.000 abstract 2
- 229960000633 dextran sulfate Drugs 0.000 abstract 2
- 235000013855 polyvinylpyrrolidone Nutrition 0.000 abstract 2
- 229920000036 polyvinylpyrrolidone Polymers 0.000 abstract 2
- 229940069328 povidone Drugs 0.000 abstract 2
- UCDLBNQQNRQANR-UHFFFAOYSA-N 4-(4-amino-3-methylphenyl)-2,6,6-trimethylcyclohexa-2,4-dien-1-amine Chemical compound CC1(C)C(N)C(C)=CC(C=2C=C(C)C(N)=CC=2)=C1 UCDLBNQQNRQANR-UHFFFAOYSA-N 0.000 abstract 1
- 235000010418 carrageenan Nutrition 0.000 abstract 1
- 239000000679 carrageenan Substances 0.000 abstract 1
- 229920001525 carrageenan Polymers 0.000 abstract 1
- 229940113118 carrageenan Drugs 0.000 abstract 1
- 239000007795 chemical reaction product Substances 0.000 abstract 1
- 229920001577 copolymer Polymers 0.000 abstract 1
- XJRBAMWJDBPFIM-UHFFFAOYSA-N methyl vinyl ether Chemical compound COC=C XJRBAMWJDBPFIM-UHFFFAOYSA-N 0.000 abstract 1
- 229920000642 polymer Polymers 0.000 abstract 1
- 239000000243 solution Substances 0.000 abstract 1
- UHVMMEOXYDMDKI-JKYCWFKZSA-L zinc;1-(5-cyanopyridin-2-yl)-3-[(1s,2s)-2-(6-fluoro-2-hydroxy-3-propanoylphenyl)cyclopropyl]urea;diacetate Chemical compound [Zn+2].CC([O-])=O.CC([O-])=O.CCC(=O)C1=CC=C(F)C([C@H]2[C@H](C2)NC(=O)NC=2N=CC(=CC=2)C#N)=C1O UHVMMEOXYDMDKI-JKYCWFKZSA-L 0.000 abstract 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54393—Improving reaction conditions or stability, e.g. by coating or irradiation of surface, by reduction of non-specific binding, by promotion of specific binding
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/26—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase
- C12Q1/28—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase involving peroxidase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2326/00—Chromogens for determinations of oxidoreductase enzymes
- C12Q2326/10—Benzidines
- C12Q2326/12—3,3',5,5'-Tetramethylbenzidine, i.e. TMB
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S435/00—Chemistry: molecular biology and microbiology
- Y10S435/962—Prevention or removal of interfering materials or reactants or other treatment to enhance results, e.g. determining or preventing nonspecific binding
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S435/00—Chemistry: molecular biology and microbiology
- Y10S435/967—Standards, controls, materials, e.g. validation studies, buffer systems
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Immunology (AREA)
- Organic Chemistry (AREA)
- Molecular Biology (AREA)
- Urology & Nephrology (AREA)
- Biotechnology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Microbiology (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Biochemistry (AREA)
- Hematology (AREA)
- Biomedical Technology (AREA)
- General Health & Medical Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Medicinal Chemistry (AREA)
- General Engineering & Computer Science (AREA)
- Cell Biology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biophysics (AREA)
- Food Science & Technology (AREA)
- Genetics & Genomics (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Acyclic And Carbocyclic Compounds In Medicinal Compositions (AREA)
- Addition Polymer Or Copolymer, Post-Treatments, Or Chemical Modifications (AREA)
- Medicinal Preparation (AREA)
- Pyrrole Compounds (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US07/431,485 US5006461A (en) | 1989-11-03 | 1989-11-03 | TMB formulation for soluble and precipitable HRP-ELISA |
| US07/470,678 US5013646A (en) | 1989-11-03 | 1990-01-26 | TMB Formulation for soluble and precipitable HRP-ELISA |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| DK0456782T3 true DK0456782T3 (da) | 1996-02-12 |
Family
ID=27029059
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DK90916953.4T DK0456782T3 (da) | 1989-11-03 | 1990-11-02 | TMB formulering til opløselig og præcipiterbar HRP-ELISA |
Country Status (9)
| Country | Link |
|---|---|
| US (1) | US5013646A (de) |
| EP (2) | EP0618300A1 (de) |
| JP (1) | JPH0743378B2 (de) |
| AT (1) | ATE133207T1 (de) |
| CA (1) | CA2045522A1 (de) |
| DE (1) | DE69024948T2 (de) |
| DK (1) | DK0456782T3 (de) |
| ES (1) | ES2085918T3 (de) |
| WO (1) | WO1991006672A1 (de) |
Families Citing this family (16)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5238817A (en) * | 1991-07-12 | 1993-08-24 | E. I. Du Pont De Nemours And Company | Chromogenic substrates for improving detection in a peroxidase-based assay |
| US5910423A (en) * | 1995-04-28 | 1999-06-08 | Ricoh Kyosan, Inc. | Water soluble powered formulation of reagent mixture containing water-insoluble reagents, and process for their production |
| WO1997005487A1 (en) | 1995-07-26 | 1997-02-13 | Universite De Montreal | ELISA SERODIAGNOSIS OF PIG PLEUROPNEUMONIA SEROTYPES 5a AND 5b |
| US5804404A (en) * | 1996-01-22 | 1998-09-08 | Dako Corporation | Stable substrate-chromogen solutions for enenzyme activity detection |
| US5792618A (en) * | 1996-09-27 | 1998-08-11 | Moss, Inc. | Liquid single-component substrates for detection or assay of horseradish peroxidase |
| ES2277376T3 (es) * | 1997-04-08 | 2007-07-01 | Universite De Montreal | Serodiagnostico mediante elisa de la pleuroneumonia porcina de serotipo 2. |
| EP1000358B1 (de) | 1997-07-15 | 2003-04-02 | Kem-En-Tec A/S | Vorgefärbte 3,3',5,5'-tetramethylbenzidin-substrate zum nachweis von enzymaktivität |
| DE10315074A1 (de) | 2003-04-02 | 2004-10-14 | Clondiag Chip Technologies Gmbh | Vorrichtung zur Vervielfältigung und zum Nachweis von Nukleinsäuren |
| WO2005022155A1 (ja) | 2003-08-29 | 2005-03-10 | Kabushiki Kaisha Toshiba | 発色試薬、濃度測定用キット、濃度測定方法及びそれに用いるセンサチップ |
| DE102005052713A1 (de) | 2005-11-04 | 2007-05-16 | Clondiag Chip Tech Gmbh | Vorrichtung und Verfahren zum Nachweis von molekularen Wechselwirkungen |
| DE102004022263A1 (de) | 2004-05-06 | 2005-12-15 | Clondiag Chip Technologies Gmbh | Vorrichtung und Verfahren zum Nachweis von molekularen Wechselwirkungen |
| US20100233697A1 (en) * | 2008-11-14 | 2010-09-16 | Helicos Biosciences Corporation | Method for standarizing surface binding of a nucleic acid sample for sequencing analysis |
| US9618429B2 (en) | 2012-01-23 | 2017-04-11 | Ventana Medical Systems, Inc. | Polymer stabilization of chromogen solutions |
| CN105116141B (zh) * | 2015-07-02 | 2016-09-14 | 深圳市卫光生物制品股份有限公司 | 一种单组份tmb显色液及其制备方法 |
| EP3368904A1 (de) * | 2015-10-30 | 2018-09-05 | Oxford University Innovation Ltd. | Peptid und biomarker im zusammenhang mit entzündungserkrankungen und verwendungen davon |
| CN117233387A (zh) * | 2023-11-15 | 2023-12-15 | 简达生物医药(南京)有限公司 | 一种杆状病毒滴度测定方法、试剂盒 |
Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4615972A (en) * | 1983-11-04 | 1986-10-07 | Immuno Concepts, Inc. | Stabilization of indicators for detecting enzyme activity |
| GB8505899D0 (en) * | 1985-03-07 | 1985-04-11 | Boots Celltech Diagnostics | Assay reagents |
| US4931385A (en) * | 1985-06-24 | 1990-06-05 | Hygeia Sciences, Incorporated | Enzyme immunoassays and immunologic reagents |
| US4673654A (en) * | 1985-10-31 | 1987-06-16 | Warner-Lambert Company | Composition for determining peroxidase-like activity of hemoglobin |
| DE3541186A1 (de) * | 1985-11-21 | 1987-05-27 | Boehringer Mannheim Gmbh | Wasserloesliche, stabilisierte peroxidasederivate, verfahren zu ihrer herstellung und verwendung zur bestimmung von wasserstoffperoxid |
-
1990
- 1990-01-26 US US07/470,678 patent/US5013646A/en not_active Expired - Lifetime
- 1990-11-02 CA CA002045522A patent/CA2045522A1/en not_active Abandoned
- 1990-11-02 EP EP94201640A patent/EP0618300A1/de not_active Ceased
- 1990-11-02 WO PCT/US1990/006493 patent/WO1991006672A1/en not_active Ceased
- 1990-11-02 EP EP90916953A patent/EP0456782B1/de not_active Expired - Lifetime
- 1990-11-02 JP JP2515721A patent/JPH0743378B2/ja not_active Expired - Fee Related
- 1990-11-02 DK DK90916953.4T patent/DK0456782T3/da active
- 1990-11-02 DE DE69024948T patent/DE69024948T2/de not_active Expired - Fee Related
- 1990-11-02 AT AT90916953T patent/ATE133207T1/de not_active IP Right Cessation
- 1990-11-02 ES ES90916953T patent/ES2085918T3/es not_active Expired - Lifetime
Also Published As
| Publication number | Publication date |
|---|---|
| JPH0743378B2 (ja) | 1995-05-15 |
| CA2045522A1 (en) | 1991-05-04 |
| EP0456782A1 (de) | 1991-11-21 |
| US5013646A (en) | 1991-05-07 |
| ES2085918T3 (es) | 1996-06-16 |
| ATE133207T1 (de) | 1996-02-15 |
| EP0456782B1 (de) | 1996-01-17 |
| JPH04503116A (ja) | 1992-06-04 |
| WO1991006672A1 (en) | 1991-05-16 |
| DE69024948D1 (de) | 1996-02-29 |
| DE69024948T2 (de) | 1996-06-20 |
| EP0618300A1 (de) | 1994-10-05 |
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