DK0677584T3 - Stabile mutanter af D-N-alpha-carbamylase - Google Patents

Stabile mutanter af D-N-alpha-carbamylase

Info

Publication number
DK0677584T3
DK0677584T3 DK95103572.4T DK95103572T DK0677584T3 DK 0677584 T3 DK0677584 T3 DK 0677584T3 DK 95103572 T DK95103572 T DK 95103572T DK 0677584 T3 DK0677584 T3 DK 0677584T3
Authority
DK
Denmark
Prior art keywords
alpha
carbamylase
mutants
preparation
wild type
Prior art date
Application number
DK95103572.4T
Other languages
English (en)
Inventor
Giuliano Galli
Renata Grifantini
Guido Grandi
Original Assignee
Eniricerche Spa
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Eniricerche Spa filed Critical Eniricerche Spa
Application granted granted Critical
Publication of DK0677584T3 publication Critical patent/DK0677584T3/da

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/78—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5)
    • C12N9/80—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5) acting on amide bonds in linear amides (3.5.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C12P13/04—Alpha- or beta- amino acids

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Peptides Or Proteins (AREA)
DK95103572.4T 1994-04-15 1995-03-13 Stabile mutanter af D-N-alpha-carbamylase DK0677584T3 (da)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
ITMI940725A IT1269321B (it) 1994-04-15 1994-04-15 Mutanti stabili della d-n- -carbamilasi

Publications (1)

Publication Number Publication Date
DK0677584T3 true DK0677584T3 (da) 1997-05-12

Family

ID=11368609

Family Applications (1)

Application Number Title Priority Date Filing Date
DK95103572.4T DK0677584T3 (da) 1994-04-15 1995-03-13 Stabile mutanter af D-N-alpha-carbamylase

Country Status (10)

Country Link
US (2) US5869298A (da)
EP (1) EP0677584B1 (da)
JP (1) JP3701341B2 (da)
AT (1) ATE146223T1 (da)
DE (1) DE69500108T2 (da)
DK (1) DK0677584T3 (da)
ES (1) ES2096494T3 (da)
GR (1) GR3022476T3 (da)
IT (1) IT1269321B (da)
SI (1) SI0677584T1 (da)

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5824522A (en) * 1990-12-07 1998-10-20 Kanegafuchi Kagaku Kogyo Kabushiki Kaisha Recombinant decarbamylases for producing D-α-amino acids
IT1274168B (it) * 1994-04-15 1997-07-15 Eniricerche Spa Vettore plasmidico e suo impiego per la produzione di proteine eterologhe
US5962279A (en) * 1994-06-24 1999-10-05 Kanegafuchi Kagaku Kogyo Kabushiki Kaisha Process for producing D-amino acids with composite immobilized enzyme preparation
IT1277125B1 (it) 1995-12-21 1997-11-04 Eniricerche Spa Mutanti termostabili della d-n-alfa-carbamilasi
US6524837B1 (en) 1999-03-29 2003-02-25 California Institute Of Technology Hydantoinase variants with improved properties and their use for the production of amino acids
EP1199355B1 (en) * 1999-06-29 2007-03-14 National Institute of Advanced Industrial Science and Technology Sulfur atom-free enzyme proteins
JP2001069981A (ja) * 1999-08-31 2001-03-21 Kanegafuchi Chem Ind Co Ltd デカルバミラーゼの立体構造およびその利用法
AU2001248278A1 (en) * 2000-04-13 2001-10-30 Novozymes A/S Extracellular expression of pectate lyase using bacillus or escherichia coli
JP4561009B2 (ja) * 2001-07-10 2010-10-13 味の素株式会社 Ｄ−ヒダントインハイドロラーゼをコードするｄｎａ、ｎ−カルバミル−ｄ−アミノ酸ハイドロラーゼをコードするｄｎａ、該遺伝子を含む組み換えｄｎａ、該組み換えｄｎａにより形質転換された細胞、該形質転換細胞を用いるタンパク質の製造方法、および、ｄ−アミノ酸の製造方法
JP4561010B2 (ja) * 2001-07-10 2010-10-13 味の素株式会社 Ｄ−ヒダントインハイドロラーゼをコードするｄｎａ、ｎ−カルバミル−ｄ−アミノ酸ハイドロラーゼをコードするｄｎａ、該遺伝子を含む組み換えｄｎａ、該組み換えｄｎａにより形質転換された細胞、該形質転換細胞を用いるタンパク質の製造方法、および、ｄ−アミノ酸の製造方法
CN115772513B (zh) * 2020-05-08 2025-05-02 江南大学 D-氨甲酰水解酶突变体、基因、表达载体及其应用

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2526965B2 (ja) * 1987-02-24 1996-08-21 武田薬品工業株式会社 ムテイン，ｄｎａおよびその用途
EP0515698B1 (en) * 1990-12-07 1998-07-29 Kanegafuchi Kagaku Kogyo Kabushiki Kaisha Process for producing D-alpha-amino acids
EP1568778A1 (en) * 1992-06-30 2005-08-31 Smithkline Beecham Plc Hydantoinase from agrobacterium
WO1994003613A1 (fr) * 1992-08-10 1994-02-17 Kanegafuchi Kagaku Kogyo Kabushiki Kaisha Adn codant pour la decarbamylase a thermostabilite accrue et utilisation de cet adn
IT1274167B (it) * 1994-04-15 1997-07-15 Eniricerche Spa Procedimento per la produzione di d- -amminoacidi

Also Published As

Publication number Publication date
EP0677584B1 (en) 1996-12-11
ITMI940725A0 (it) 1994-04-15
ATE146223T1 (de) 1996-12-15
GR3022476T3 (en) 1997-05-31
JP3701341B2 (ja) 2005-09-28
US5869298A (en) 1999-02-09
DE69500108D1 (de) 1997-01-23
DE69500108T2 (de) 1997-06-12
US5807710A (en) 1998-09-15
EP0677584A1 (en) 1995-10-18
ES2096494T3 (es) 1997-03-01
ITMI940725A1 (it) 1995-10-15
JPH0884584A (ja) 1996-04-02
IT1269321B (it) 1997-03-26
SI0677584T1 (en) 1997-10-31

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