DK0779931T3 - Fremgangsmåder til udførelse af calciumphosphattransfektion - Google Patents
Fremgangsmåder til udførelse af calciumphosphattransfektionInfo
- Publication number
- DK0779931T3 DK0779931T3 DK95932364T DK95932364T DK0779931T3 DK 0779931 T3 DK0779931 T3 DK 0779931T3 DK 95932364 T DK95932364 T DK 95932364T DK 95932364 T DK95932364 T DK 95932364T DK 0779931 T3 DK0779931 T3 DK 0779931T3
- Authority
- DK
- Denmark
- Prior art keywords
- calcium phosphate
- phosphate transfection
- methods
- host cell
- particles
- Prior art date
Links
- 229910000389 calcium phosphate Inorganic materials 0.000 title abstract 3
- 239000001506 calcium phosphate Substances 0.000 title abstract 3
- 235000011010 calcium phosphates Nutrition 0.000 title abstract 3
- QORWJWZARLRLPR-UHFFFAOYSA-H tricalcium bis(phosphate) Chemical compound [Ca+2].[Ca+2].[Ca+2].[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O QORWJWZARLRLPR-UHFFFAOYSA-H 0.000 title abstract 3
- 238000000034 method Methods 0.000 title abstract 2
- 238000001890 transfection Methods 0.000 title abstract 2
- 239000002245 particle Substances 0.000 abstract 3
- 150000007523 nucleic acids Chemical class 0.000 abstract 1
- 102000039446 nucleic acids Human genes 0.000 abstract 1
- 108020004707 nucleic acids Proteins 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
Landscapes
- Genetics & Genomics (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Organic Chemistry (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biophysics (AREA)
- Microbiology (AREA)
- Plant Pathology (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Physics & Mathematics (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
- Coloring Foods And Improving Nutritive Qualities (AREA)
- Materials For Medical Uses (AREA)
- Catalysts (AREA)
- Fuel Cell (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US08/303,245 US5484720A (en) | 1994-09-08 | 1994-09-08 | Methods for calcium phosphate transfection |
| US08/430,533 US5593875A (en) | 1994-09-08 | 1995-04-26 | Methods for calcium phosphate transfection |
| PCT/US1995/011088 WO1996007750A1 (en) | 1994-09-08 | 1995-08-30 | Methods for calcium phosphate transfection |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| DK0779931T3 true DK0779931T3 (da) | 2003-03-03 |
Family
ID=26973345
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DK95932364T DK0779931T3 (da) | 1994-09-08 | 1995-08-30 | Fremgangsmåder til udførelse af calciumphosphattransfektion |
Country Status (12)
| Country | Link |
|---|---|
| EP (1) | EP0779931B1 (da) |
| JP (1) | JP3815794B2 (da) |
| AT (1) | ATE228170T1 (da) |
| AU (1) | AU694800B2 (da) |
| CA (1) | CA2198589C (da) |
| DE (1) | DE69528903T2 (da) |
| DK (1) | DK0779931T3 (da) |
| ES (1) | ES2187574T3 (da) |
| IL (1) | IL115178A0 (da) |
| MX (1) | MX9701708A (da) |
| PT (1) | PT779931E (da) |
| WO (1) | WO1996007750A1 (da) |
Families Citing this family (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB9515356D0 (en) * | 1995-07-26 | 1995-09-20 | Medical Res Council | Improvements in or relating to delivery of nucleic acid |
| ATE347587T1 (de) | 1996-12-24 | 2006-12-15 | Nestle Sa | Immortalisierte menschliche epithelzell-linie aus kornea |
| US6635457B1 (en) | 1999-07-30 | 2003-10-21 | Stratagene | Selection for more efficient transformation host cells |
| DE10024334B4 (de) * | 2000-05-17 | 2006-06-01 | Medigene Ag | Verfahren zum Einbringen von Nukleinsäure in eine Zelle (Transfektion) mittels Calciumphosphat |
| EP1270733A1 (en) * | 2001-06-21 | 2003-01-02 | Ecole Polytechnique Federale De Lausanne | Method for introducing a nucleic acid into a eukaryotic cell |
| WO2003000908A1 (en) * | 2001-06-21 | 2003-01-03 | Ecole Polytechnique Federale De Lausanne | A method for introducing a nucleic acid into a eukaryotic cell |
| IL161541A0 (en) | 2001-10-22 | 2004-09-27 | Applied Research Systems | Mutant glycoproteins |
| US9493744B2 (en) * | 2012-06-20 | 2016-11-15 | Genentech, Inc. | Methods for viral inactivation and other adventitious agents |
| JP2021533831A (ja) * | 2018-08-16 | 2021-12-09 | スペースクラフト セブン リミテッド ライアビリティ カンパニー | ウイルスベクターのための生成方法 |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0195592B1 (en) * | 1985-03-15 | 1992-04-22 | Btg International Limited | Factor ix protein |
-
1995
- 1995-08-30 ES ES95932364T patent/ES2187574T3/es not_active Expired - Lifetime
- 1995-08-30 EP EP95932364A patent/EP0779931B1/en not_active Expired - Lifetime
- 1995-08-30 AU AU35429/95A patent/AU694800B2/en not_active Expired
- 1995-08-30 WO PCT/US1995/011088 patent/WO1996007750A1/en not_active Ceased
- 1995-08-30 DK DK95932364T patent/DK0779931T3/da active
- 1995-08-30 DE DE69528903T patent/DE69528903T2/de not_active Expired - Lifetime
- 1995-08-30 JP JP50958496A patent/JP3815794B2/ja not_active Expired - Lifetime
- 1995-08-30 AT AT95932364T patent/ATE228170T1/de active
- 1995-08-30 MX MX9701708A patent/MX9701708A/es unknown
- 1995-08-30 CA CA2198589A patent/CA2198589C/en not_active Expired - Lifetime
- 1995-08-31 PT PT95932364T patent/PT779931E/pt unknown
- 1995-09-05 IL IL11517895A patent/IL115178A0/xx not_active IP Right Cessation
Also Published As
| Publication number | Publication date |
|---|---|
| PT779931E (pt) | 2003-04-30 |
| AU3542995A (en) | 1996-03-27 |
| ES2187574T3 (es) | 2003-06-16 |
| MX9701708A (es) | 1997-05-31 |
| JP3815794B2 (ja) | 2006-08-30 |
| DE69528903T2 (de) | 2003-07-24 |
| CA2198589C (en) | 2010-06-15 |
| WO1996007750A1 (en) | 1996-03-14 |
| EP0779931B1 (en) | 2002-11-20 |
| CA2198589A1 (en) | 1996-03-14 |
| DE69528903D1 (de) | 2003-01-02 |
| ATE228170T1 (de) | 2002-12-15 |
| EP0779931A1 (en) | 1997-06-25 |
| AU694800B2 (en) | 1998-07-30 |
| JPH10508462A (ja) | 1998-08-25 |
| IL115178A0 (en) | 1995-12-31 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| EP1249497A3 (en) | Methods for calcium phosphate transfection | |
| FR2543158B1 (fr) | Milieu de culture de cellules animales sans serum, sans hormones et sans facteurs de croissance et procedes de culture primaire et d'obtention de lignees cellulaires utilisant ce milieu | |
| ATE153214T1 (de) | Medien zur zellzucht und verfahren dazu | |
| DE69325389D1 (de) | Verfahren zur stabile transformation von weizen | |
| ATE27463T1 (de) | Verfahren zur in vitro transformation von pflanzenprotoplasten mit plasmid-dns. | |
| PL328922A1 (en) | Culture medium for growing mammal tissue cells | |
| MX9700852A (es) | Metodo para seleccionar celulas huesped de alta expresion. | |
| AU7213294A (en) | Methods of culturing and modulating the growth of hair follicular stem cells | |
| ATE228170T1 (de) | Verfahren zur kalziumphosphat-transfektion | |
| AUPP421298A0 (en) | Method and medium for in vitro culture of human embryos | |
| WO2003089581A3 (en) | A method to increase the rate of cell growth | |
| DK1141366T3 (da) | Transfektaconer omfattende calciumphosphat og en nukleinsyre | |
| AU570954B2 (en) | Propagation of proliferating cell aggregates | |
| DK0755438T3 (da) | Fremgangsmåde til bekæmpelse af metallophosphatpræcipitation i fermenteringer med høj celledensitet | |
| AU574971B2 (en) | Cell separation | |
| GEP19991853B (en) | Stable Arylpyrrole Particles, Process for Their Preparation and Suspension Concentrate Compositions Comprising Them | |
| AU7677187A (en) | Process for the immortalization of human and animal cells | |
| PL211045A1 (pl) | Srodek do regulowania wzrostu roslin oraz sposob wytwarzania soli kwasow alfa-izocyjanokarboksylowych | |
| AU6262786A (en) | Growing aids virus in cell lines lacking human class ii histocompatability antigens | |
| AU2182801A (en) | Method for producing adherent animal cells | |
| WO1994013800A3 (en) | Mk protein preparation and use in cell culture | |
| IL75118A0 (en) | Additives for use in cell culture,culture medium for non-serum cultivation and method of cell culture using them | |
| ES8708219A1 (es) | Un procedimiento para la preparacion de oxetanonas | |
| EP0263659A3 (en) | Tissue culture media containing l-carnitine | |
| AU6112386A (en) | Method for enhancement of growth of human cells in culture |