DK0840746T3 - Fremgangsmåde til oprensning af proteiner - Google Patents
Fremgangsmåde til oprensning af proteinerInfo
- Publication number
- DK0840746T3 DK0840746T3 DK96927033T DK96927033T DK0840746T3 DK 0840746 T3 DK0840746 T3 DK 0840746T3 DK 96927033 T DK96927033 T DK 96927033T DK 96927033 T DK96927033 T DK 96927033T DK 0840746 T3 DK0840746 T3 DK 0840746T3
- Authority
- DK
- Denmark
- Prior art keywords
- pct
- proteins
- purification
- sec
- deionized water
- Prior art date
Links
- 102000004169 proteins and genes Human genes 0.000 title abstract 3
- 108090000623 proteins and genes Proteins 0.000 title abstract 3
- 238000000034 method Methods 0.000 title abstract 2
- 238000000746 purification Methods 0.000 title abstract 2
- 239000008367 deionised water Substances 0.000 abstract 2
- 229910021641 deionized water Inorganic materials 0.000 abstract 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 abstract 2
- RYGMFSIKBFXOCR-UHFFFAOYSA-N Copper Chemical compound [Cu] RYGMFSIKBFXOCR-UHFFFAOYSA-N 0.000 abstract 1
- JPVYNHNXODAKFH-UHFFFAOYSA-N Cu2+ Chemical compound [Cu+2] JPVYNHNXODAKFH-UHFFFAOYSA-N 0.000 abstract 1
- 239000002879 Lewis base Substances 0.000 abstract 1
- 238000001042 affinity chromatography Methods 0.000 abstract 1
- 229910052802 copper Inorganic materials 0.000 abstract 1
- 239000010949 copper Substances 0.000 abstract 1
- 150000007527 lewis bases Chemical class 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
- C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
- C12N9/2405—Glucanases
- C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
- C12N9/2411—Amylases
- C12N9/2414—Alpha-amylase (3.2.1.1.)
- C12N9/2417—Alpha-amylase (3.2.1.1.) from microbiological source
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/16—Extraction; Separation; Purification by chromatography
- C07K1/22—Affinity chromatography or related techniques based upon selective absorption processes
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/52—Cytokines; Lymphokines; Interferons
- C07K14/555—Interferons [IFN]
- C07K14/56—IFN-alpha
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
- C12N9/6421—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
- C12N9/6424—Serine endopeptidases (3.4.21)
- C12N9/6427—Chymotrypsins (3.4.21.1; 3.4.21.2); Trypsin (3.4.21.4)
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S530/00—Chemistry: natural resins or derivatives; peptides or proteins; lignins or reaction products thereof
- Y10S530/81—Carrier - bound or immobilized peptides or proteins and the preparation thereof, e.g. biological cell or cell fragment as carrier
- Y10S530/811—Peptides or proteins is immobilized on, or in, an inorganic carrier
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S530/00—Chemistry: natural resins or derivatives; peptides or proteins; lignins or reaction products thereof
- Y10S530/81—Carrier - bound or immobilized peptides or proteins and the preparation thereof, e.g. biological cell or cell fragment as carrier
- Y10S530/812—Peptides or proteins is immobilized on, or in, an organic carrier
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Zoology (AREA)
- Molecular Biology (AREA)
- General Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Biochemistry (AREA)
- Medicinal Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Microbiology (AREA)
- General Engineering & Computer Science (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Toxicology (AREA)
- Gastroenterology & Hepatology (AREA)
- Analytical Chemistry (AREA)
- Peptides Or Proteins (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Enzymes And Modification Thereof (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP95810485 | 1995-07-26 | ||
| EP96927033A EP0840746B1 (en) | 1995-07-26 | 1996-07-17 | Method for the purification of proteins |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| DK0840746T3 true DK0840746T3 (da) | 2004-11-29 |
Family
ID=8221776
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DK96927033T DK0840746T3 (da) | 1995-07-26 | 1996-07-17 | Fremgangsmåde til oprensning af proteiner |
Country Status (13)
| Country | Link |
|---|---|
| US (1) | US5990289A (da) |
| EP (1) | EP0840746B1 (da) |
| JP (1) | JP3833255B2 (da) |
| AT (1) | ATE278705T1 (da) |
| AU (1) | AU6700796A (da) |
| CA (1) | CA2226080A1 (da) |
| DE (1) | DE69633567T2 (da) |
| DK (1) | DK0840746T3 (da) |
| ES (1) | ES2227603T3 (da) |
| PT (1) | PT840746E (da) |
| TW (1) | TW442492B (da) |
| WO (1) | WO1997005159A2 (da) |
| ZA (1) | ZA966315B (da) |
Families Citing this family (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6479300B1 (en) * | 1999-03-15 | 2002-11-12 | Millipore Corporation | Metal loaded ligand bound membranes for metal ion affinity chromatography |
| US7128904B2 (en) * | 2001-01-16 | 2006-10-31 | The Regents Of The University Of Michigan | Material containing metal ion ligand complex producing nitric oxide in contact with blood |
| US7335383B2 (en) * | 2001-01-16 | 2008-02-26 | The Regents Of The University Of Michigan | Generation of nitric oxide in vivo from nitrite, nitrate or nitrosothiols endogenous in blood |
| ATE394412T1 (de) * | 2001-06-21 | 2008-05-15 | Clontech Lab Inc | Wasserlösliche polymere zusammensetzungen mit affinität für metallionen und verfahren zu deren anwendung |
| CN1596268A (zh) * | 2001-11-28 | 2005-03-16 | 桑多斯有限公司 | 重组人红细胞生成素的层析纯化 |
| US7294614B2 (en) * | 2004-10-12 | 2007-11-13 | Clontech Laboratories, Inc. | Phosphoprotein affinity resins and methods for making and using the same |
| WO2007005758A2 (en) * | 2005-06-30 | 2007-01-11 | Mc3, Inc. | Methods , compositions and devices for promoting angiogenesis |
| CA2632224A1 (en) * | 2005-12-02 | 2007-06-07 | The Regents Of The University Of Michigan | Polymer compositions, coatings and devices, and methods of making and using the same |
| AU2011343813B2 (en) | 2010-12-15 | 2015-05-21 | Takeda Pharmaceutical Company Limited | Eluate collection using conductivity gradient |
Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| ES506955A0 (es) * | 1980-11-10 | 1983-02-01 | Genentech Inc | Un procedimiento para producir un polipeptido antiviral. |
| DE3685996T2 (de) * | 1985-06-11 | 1993-01-14 | Ciba Geigy Ag | Hybrid-interferone. |
| CA1304886C (en) * | 1986-07-10 | 1992-07-07 | Heinz Dobeli | Metal chelate resins |
| US5169936A (en) * | 1989-04-14 | 1992-12-08 | Biogen, Inc. | Protein purification on immobilized metal affinity resins effected by elution using a weak ligand |
| US5136025A (en) * | 1990-04-04 | 1992-08-04 | California Biotechnology Inc. | Method to purify basic fibroblast growth factor |
-
1996
- 1996-07-12 TW TW085108439A patent/TW442492B/zh not_active IP Right Cessation
- 1996-07-17 JP JP50718397A patent/JP3833255B2/ja not_active Expired - Fee Related
- 1996-07-17 CA CA002226080A patent/CA2226080A1/en not_active Abandoned
- 1996-07-17 DE DE69633567T patent/DE69633567T2/de not_active Expired - Fee Related
- 1996-07-17 PT PT96927033T patent/PT840746E/pt unknown
- 1996-07-17 EP EP96927033A patent/EP0840746B1/en not_active Expired - Lifetime
- 1996-07-17 ES ES96927033T patent/ES2227603T3/es not_active Expired - Lifetime
- 1996-07-17 DK DK96927033T patent/DK0840746T3/da active
- 1996-07-17 AU AU67007/96A patent/AU6700796A/en not_active Abandoned
- 1996-07-17 US US09/000,031 patent/US5990289A/en not_active Expired - Fee Related
- 1996-07-17 WO PCT/EP1996/003144 patent/WO1997005159A2/en not_active Ceased
- 1996-07-17 AT AT96927033T patent/ATE278705T1/de not_active IP Right Cessation
- 1996-07-25 ZA ZA966315A patent/ZA966315B/xx unknown
Also Published As
| Publication number | Publication date |
|---|---|
| EP0840746B1 (en) | 2004-10-06 |
| WO1997005159A3 (en) | 1997-03-06 |
| JP3833255B2 (ja) | 2006-10-11 |
| JPH11509853A (ja) | 1999-08-31 |
| WO1997005159A2 (en) | 1997-02-13 |
| DE69633567T2 (de) | 2006-02-23 |
| PT840746E (pt) | 2004-12-31 |
| CA2226080A1 (en) | 1997-02-13 |
| AU6700796A (en) | 1997-02-26 |
| EP0840746A2 (en) | 1998-05-13 |
| DE69633567D1 (de) | 2004-11-11 |
| ES2227603T3 (es) | 2005-04-01 |
| ZA966315B (en) | 1997-01-27 |
| ATE278705T1 (de) | 2004-10-15 |
| TW442492B (en) | 2001-06-23 |
| US5990289A (en) | 1999-11-23 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| DK1075488T3 (da) | Proteinoprensning ved ionbytningskromatografi | |
| DE69430016D1 (de) | Proteinreinigung | |
| MX9603637A (es) | Purificacion de anticuerpo. | |
| DK0877090T3 (da) | Fremgangsmåde til produktion af L-aminosyrer | |
| DK0620854T3 (da) | Ekspression og oprensning af rekombinant opløselig vævsfaktor | |
| ATE425989T1 (de) | Proteinreinigungsverfahren | |
| DK0565168T3 (da) | Fremgangsmåde til fremstilling af oprenset enzym | |
| EP0131142A3 (en) | Process for recovering a cell wall protein | |
| DK0789767T3 (da) | Fremgangsmåde til fremstilling af oprensede papilomavirusproteiner | |
| ZA966315B (en) | Method for the purification of proteins | |
| DK0701623T3 (da) | Fremgangsmåde til fremstilling af N-acetyl-neuraminsyre | |
| ATE285474T1 (de) | Haemophilus adhäsionsproteinen | |
| IL122606A (en) | (S)-hydroxa-nitrile-lyase | |
| DK0635025T3 (da) | Fremgangsmåde til fremstilling af modificerede proteiner | |
| ATE294189T1 (de) | Kristallisation von proteinen | |
| ATE202798T1 (de) | D-aminosäure-oxidase kodierendes dns-fragment | |
| EP0735140A3 (en) | Process for secretory protein production | |
| DK0401657T3 (da) | Fremgangsmåde til C-terminal modificering af proteiner | |
| NO20005819L (no) | Nye peptidfragmenter for rensing av proteiner | |
| DK162604C (da) | Fremgangsmaade til fremstilling af humant immuninterferon samt farmaceutiske praeparater indeholdende immuninterferon fremstillet ved fremgangsmaaden ifoelge opfindelsen og anvendelse af humant interferon fremstillet ved fremgangsmaaden ifoelge opfindelsen til fremstilling af et terapeutisk middel | |
| KR970009339B1 (en) | Tpa from scolopendra subepinpes multilans and its purification method | |
| KR970006498A (ko) | 활성형 인성장호르몬의 정제 방법 | |
| AR002059A1 (es) | Procedimiento para aislar y purificar una proteina de plasma humano con actividad fibrinolitica |