DK0840746T3 - Fremgangsmåde til oprensning af proteiner - Google Patents

Fremgangsmåde til oprensning af proteiner

Info

Publication number
DK0840746T3
DK0840746T3 DK96927033T DK96927033T DK0840746T3 DK 0840746 T3 DK0840746 T3 DK 0840746T3 DK 96927033 T DK96927033 T DK 96927033T DK 96927033 T DK96927033 T DK 96927033T DK 0840746 T3 DK0840746 T3 DK 0840746T3
Authority
DK
Denmark
Prior art keywords
pct
proteins
purification
sec
deionized water
Prior art date
Application number
DK96927033T
Other languages
English (en)
Inventor
Catherine Georges
Pierre Francois Fauquex
Original Assignee
Novartis Ag
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Novartis Ag filed Critical Novartis Ag
Application granted granted Critical
Publication of DK0840746T3 publication Critical patent/DK0840746T3/da

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/24Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405Glucanases
    • C12N9/2408Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411Amylases
    • C12N9/2414Alpha-amylase (3.2.1.1.)
    • C12N9/2417Alpha-amylase (3.2.1.1.) from microbiological source
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K1/00General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14Extraction; Separation; Purification
    • C07K1/16Extraction; Separation; Purification by chromatography
    • C07K1/22Affinity chromatography or related techniques based upon selective absorption processes
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K14/00Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/52Cytokines; Lymphokines; Interferons
    • C07K14/555Interferons [IFN]
    • C07K14/56IFN-alpha
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/48Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6424Serine endopeptidases (3.4.21)
    • C12N9/6427Chymotrypsins (3.4.21.1; 3.4.21.2); Trypsin (3.4.21.4)
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10STECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S530/00Chemistry: natural resins or derivatives; peptides or proteins; lignins or reaction products thereof
    • Y10S530/81Carrier - bound or immobilized peptides or proteins and the preparation thereof, e.g. biological cell or cell fragment as carrier
    • Y10S530/811Peptides or proteins is immobilized on, or in, an inorganic carrier
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10STECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S530/00Chemistry: natural resins or derivatives; peptides or proteins; lignins or reaction products thereof
    • Y10S530/81Carrier - bound or immobilized peptides or proteins and the preparation thereof, e.g. biological cell or cell fragment as carrier
    • Y10S530/812Peptides or proteins is immobilized on, or in, an organic carrier

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Toxicology (AREA)
  • Analytical Chemistry (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
DK96927033T 1995-07-26 1996-07-17 Fremgangsmåde til oprensning af proteiner DK0840746T3 (da)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP95810485 1995-07-26
EP96927033A EP0840746B1 (en) 1995-07-26 1996-07-17 Method for the purification of proteins

Publications (1)

Publication Number Publication Date
DK0840746T3 true DK0840746T3 (da) 2004-11-29

Family

ID=8221776

Family Applications (1)

Application Number Title Priority Date Filing Date
DK96927033T DK0840746T3 (da) 1995-07-26 1996-07-17 Fremgangsmåde til oprensning af proteiner

Country Status (13)

Country Link
US (1) US5990289A (da)
EP (1) EP0840746B1 (da)
JP (1) JP3833255B2 (da)
AT (1) ATE278705T1 (da)
AU (1) AU6700796A (da)
CA (1) CA2226080A1 (da)
DE (1) DE69633567T2 (da)
DK (1) DK0840746T3 (da)
ES (1) ES2227603T3 (da)
PT (1) PT840746E (da)
TW (1) TW442492B (da)
WO (1) WO1997005159A2 (da)
ZA (1) ZA966315B (da)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6479300B1 (en) * 1999-03-15 2002-11-12 Millipore Corporation Metal loaded ligand bound membranes for metal ion affinity chromatography
US7128904B2 (en) * 2001-01-16 2006-10-31 The Regents Of The University Of Michigan Material containing metal ion ligand complex producing nitric oxide in contact with blood
US7335383B2 (en) * 2001-01-16 2008-02-26 The Regents Of The University Of Michigan Generation of nitric oxide in vivo from nitrite, nitrate or nitrosothiols endogenous in blood
AU2002315414B2 (en) * 2001-06-21 2008-02-07 Clontech Laboratories, Inc. Water-soluble polymeric metal ion affinity compositions and methods for using the same
EP1453857B1 (en) 2001-11-28 2014-08-13 Sandoz AG Chromatographic purification of recombinant human erythropoietin
WO2006042263A2 (en) * 2004-10-12 2006-04-20 Clontech Laboratories, Inc. Phosphoprotein affinity resins and methods for making and using the same
US20080241208A1 (en) * 2005-06-30 2008-10-02 Charles Shanley Methods, Compositions and Devices For Promoting Anglogenesis
JP2009518069A (ja) * 2005-12-02 2009-05-07 ザ・リージェンツ・オブ・ザ・ユニバーシティ・オブ・ミシガン 組成物、コーティング及びデバイス、並びにそれらの製造方法及び使用方法
SG191186A1 (en) 2010-12-15 2013-07-31 Baxter Int Eluate collection using conductivity gradient

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES506955A0 (es) * 1980-11-10 1983-02-01 Genentech Inc Un procedimiento para producir un polipeptido antiviral.
EP0205404B1 (en) * 1985-06-11 1992-07-15 Ciba-Geigy Ag Hybrid interferons
CA1304886C (en) * 1986-07-10 1992-07-07 Heinz Dobeli Metal chelate resins
US5169936A (en) * 1989-04-14 1992-12-08 Biogen, Inc. Protein purification on immobilized metal affinity resins effected by elution using a weak ligand
US5136025A (en) * 1990-04-04 1992-08-04 California Biotechnology Inc. Method to purify basic fibroblast growth factor

Also Published As

Publication number Publication date
AU6700796A (en) 1997-02-26
JP3833255B2 (ja) 2006-10-11
TW442492B (en) 2001-06-23
EP0840746A2 (en) 1998-05-13
DE69633567D1 (de) 2004-11-11
ES2227603T3 (es) 2005-04-01
US5990289A (en) 1999-11-23
ZA966315B (en) 1997-01-27
ATE278705T1 (de) 2004-10-15
JPH11509853A (ja) 1999-08-31
PT840746E (pt) 2004-12-31
CA2226080A1 (en) 1997-02-13
DE69633567T2 (de) 2006-02-23
EP0840746B1 (en) 2004-10-06
WO1997005159A2 (en) 1997-02-13
WO1997005159A3 (en) 1997-03-06

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