DK0874057T3 - Vektor og pattedyrcellelinie med forbedret produktionsevne - Google Patents

Vektor og pattedyrcellelinie med forbedret produktionsevne

Info

Publication number
DK0874057T3
DK0874057T3 DK98108577T DK98108577T DK0874057T3 DK 0874057 T3 DK0874057 T3 DK 0874057T3 DK 98108577 T DK98108577 T DK 98108577T DK 98108577 T DK98108577 T DK 98108577T DK 0874057 T3 DK0874057 T3 DK 0874057T3
Authority
DK
Denmark
Prior art keywords
cell line
vector
promoter
mammalian cell
improved production
Prior art date
Application number
DK98108577T
Other languages
Danish (da)
English (en)
Inventor
Sham-Yuen Chan
Original Assignee
Bayer Ag
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Bayer Ag filed Critical Bayer Ag
Application granted granted Critical
Publication of DK0874057T3 publication Critical patent/DK0874057T3/da

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
    • C12N15/69—Increasing the copy number of the vector
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/745—Blood coagulation or fibrinolysis factors
    • C07K14/755—Factors VIII, e.g. factor VIII C (AHF), factor VIII Ag (VWF)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Hematology (AREA)
  • Toxicology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Ultra Sonic Daignosis Equipment (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
DK98108577T 1993-06-10 1994-05-30 Vektor og pattedyrcellelinie med forbedret produktionsevne DK0874057T3 (da)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US7520993A 1993-06-10 1993-06-10
EP94108283A EP0629700A3 (fr) 1993-06-10 1994-05-30 Vecteur et lignée cellulaire de mammifères ayant une productivité améliorée.

Publications (1)

Publication Number Publication Date
DK0874057T3 true DK0874057T3 (da) 2004-11-22

Family

ID=22124251

Family Applications (1)

Application Number Title Priority Date Filing Date
DK98108577T DK0874057T3 (da) 1993-06-10 1994-05-30 Vektor og pattedyrcellelinie med forbedret produktionsevne

Country Status (8)

Country Link
US (1) US5612213A (fr)
EP (2) EP0874057B1 (fr)
JP (1) JPH06343476A (fr)
AT (1) ATE272122T1 (fr)
CA (1) CA2125449C (fr)
DE (1) DE69433925T2 (fr)
DK (1) DK0874057T3 (fr)
ES (1) ES2226029T3 (fr)

Families Citing this family (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ATE319841T1 (de) * 1996-06-12 2006-03-15 Japan Tobacco Inc Verfahren zur expression von fremdgenen und entsprechende vektoren
US5854021A (en) * 1997-02-28 1998-12-29 Bayer Corporation Enhance protein production method
US6475725B1 (en) 1997-06-20 2002-11-05 Baxter Aktiengesellschaft Recombinant cell clones having increased stability and methods of making and using the same
US6051401A (en) * 1998-07-28 2000-04-18 Bayer Corporation Methods and constructs for protein expression
US6221349B1 (en) 1998-10-20 2001-04-24 Avigen, Inc. Adeno-associated vectors for expression of factor VIII by target cells
US6358703B1 (en) * 1998-12-10 2002-03-19 Bayer Corporation Expression system for factor VIII
US20040235715A1 (en) * 1999-11-12 2004-11-25 Bayer Corporation Method of producing glycosylated bikunin
ES2338213T3 (es) 2000-06-27 2010-05-05 Basf Se Fragmentos de acido nucleico de cianobacterias que codifican proteinas utiles para controlar rasgos de plantas mediante transformacion nuclear o de plastoma.
US7723102B2 (en) 2000-09-28 2010-05-25 Bayer Corporation Enhanced transfection system
HUE054805T2 (hu) 2003-09-30 2021-09-28 Univ Pennsylvania Adeno-asszociált vírus (AAV) kládok, szekvenciák, az azokat tartalmazó vektorok és alkalmazásaik
SI1673391T1 (sl) * 2003-10-16 2009-06-30 Claude Negrier Modificirana cDNA za visoke ekspresijske nivoje faktorja VIII in njegovih derivatov
EP3085389A1 (fr) 2005-04-07 2016-10-26 The Trustees Of The University Of Pennsylvania Procédé d'augmentation de la fonction d'un vecteur aav
US20070214041A1 (en) * 2006-03-10 2007-09-13 Cisco Technologies, Inc. System and method for location-based mapping of soft-keys on a mobile communication device
KR20130040844A (ko) 2010-03-29 2013-04-24 더 트러스티스 오브 더 유니버시티 오브 펜실바니아 약학적으로 유발된 전이유전자 제거 시스템
US9315825B2 (en) 2010-03-29 2016-04-19 The Trustees Of The University Of Pennsylvania Pharmacologically induced transgene ablation system
SG10202007803QA (en) 2011-02-17 2020-09-29 Univ Pennsylvania Compositions and methods for altering tissue specificity and improving aav9-mediated gene transfer
WO2015012924A2 (fr) 2013-04-29 2015-01-29 The Trustees Of The University Of Pennsylvania Cassettes d'expression préférentielle pour tissus modifiées par un codon, vecteurs les contenant, et utilisation
EP3283126B1 (fr) 2015-04-16 2019-11-06 Emory University Promoteurs et vecteurs recombinés pour l'expression de protéines dans le foie et utilisation associée
KR20230042754A (ko) 2020-08-07 2023-03-29 아미쿠스 세라퓨틱스, 인코포레이티드 소포 표적화 단백질 및 이의 용도

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5079159A (en) * 1983-12-27 1992-01-07 Genetics Institute, Inc. Method for making tissue plasminogen activator
US4740461A (en) * 1983-12-27 1988-04-26 Genetics Institute, Inc. Vectors and methods for transformation of eucaryotic cells
US4965199A (en) * 1984-04-20 1990-10-23 Genentech, Inc. Preparation of functional human factor VIII in mammalian cells using methotrexate based selection
GB2197321B (en) * 1986-09-12 1990-10-03 Genentech Inc Method and vectors for obtaining continuous production of heterologous protein in a eukaryotic host cell line

Also Published As

Publication number Publication date
EP0629700A3 (fr) 1995-02-15
DE69433925T2 (de) 2005-07-28
EP0874057B1 (fr) 2004-07-28
EP0874057A2 (fr) 1998-10-28
CA2125449A1 (fr) 1994-12-11
ATE272122T1 (de) 2004-08-15
US5612213A (en) 1997-03-18
CA2125449C (fr) 2010-02-23
ES2226029T3 (es) 2005-03-16
EP0629700A2 (fr) 1994-12-21
DE69433925D1 (de) 2004-09-02
EP0874057A3 (fr) 1999-01-07
JPH06343476A (ja) 1994-12-20

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