DK0973874T3 - Enzymassay for mutant ildflue-luciferase - Google Patents

Enzymassay for mutant ildflue-luciferase

Info

Publication number
DK0973874T3
DK0973874T3 DK98915002T DK98915002T DK0973874T3 DK 0973874 T3 DK0973874 T3 DK 0973874T3 DK 98915002 T DK98915002 T DK 98915002T DK 98915002 T DK98915002 T DK 98915002T DK 0973874 T3 DK0973874 T3 DK 0973874T3
Authority
DK
Denmark
Prior art keywords
atp
disclosed
assaying
luciferase
firefly luciferase
Prior art date
Application number
DK98915002T
Other languages
English (en)
Inventor
Peter John White
Christopher Robin Lowe
James Augustus Henry Murray
David James Squirrell
Original Assignee
Secr Defence
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Secr Defence filed Critical Secr Defence
Application granted granted Critical
Publication of DK0973874T3 publication Critical patent/DK0973874T3/da

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0069—Oxidoreductases (1.) acting on single donors with incorporation of molecular oxygen, i.e. oxygenases (1.13)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y113/00—Oxidoreductases acting on single donors with incorporation of molecular oxygen (oxygenases) (1.13)
    • C12Y113/12—Oxidoreductases acting on single donors with incorporation of molecular oxygen (oxygenases) (1.13) with incorporation of one atom of oxygen (internal monooxygenases or internal mixed function oxidases)(1.13.12)
    • C12Y113/12007—Photinus-luciferin 4-monooxygenase (ATP-hydrolysing) (1.13.12.7), i.e. firefly-luciferase
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/81—Packaged device or kit

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Acyclic And Carbocyclic Compounds In Medicinal Compositions (AREA)
DK98915002T 1997-04-11 1998-04-07 Enzymassay for mutant ildflue-luciferase DK0973874T3 (da)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GBGB9707486.8A GB9707486D0 (en) 1997-04-11 1997-04-11 Enzyme assays
PCT/GB1998/001026 WO1998046729A2 (en) 1997-04-11 1998-04-07 Enzyme assay for mutant firefly luciferase

Publications (1)

Publication Number Publication Date
DK0973874T3 true DK0973874T3 (da) 2003-05-19

Family

ID=10810734

Family Applications (1)

Application Number Title Priority Date Filing Date
DK98915002T DK0973874T3 (da) 1997-04-11 1998-04-07 Enzymassay for mutant ildflue-luciferase

Country Status (13)

Country Link
US (1) US6265177B1 (da)
EP (1) EP0973874B1 (da)
JP (1) JP4302777B2 (da)
AT (1) ATE233810T1 (da)
AU (1) AU731408B2 (da)
CA (1) CA2283287C (da)
DE (1) DE69811876T2 (da)
DK (1) DK0973874T3 (da)
ES (1) ES2194309T3 (da)
GB (1) GB9707486D0 (da)
NZ (1) NZ337373A (da)
PT (1) PT973874E (da)
WO (1) WO1998046729A2 (da)

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US6602677B1 (en) * 1997-09-19 2003-08-05 Promega Corporation Thermostable luciferases and methods of production
WO1999014336A2 (en) * 1997-09-19 1999-03-25 Promega Corporation Thermostable luciferases and methods of production
GB9823468D0 (en) 1998-10-28 1998-12-23 Secr Defence Novel enzyme
GB9925161D0 (en) 1999-10-26 1999-12-22 Secr Defence Novel enzyme
ATE374820T1 (de) * 1999-10-26 2007-10-15 Secr Defence Mutantenluciferase
US7879540B1 (en) 2000-08-24 2011-02-01 Promega Corporation Synthetic nucleic acid molecule compositions and methods of preparation
GB0030727D0 (en) 2000-12-15 2001-01-31 Lumitech Uk Ltd Methods and kits for detecting kinase activity
US6902921B2 (en) * 2001-10-30 2005-06-07 454 Corporation Sulfurylase-luciferase fusion proteins and thermostable sulfurylase
US20050124022A1 (en) * 2001-10-30 2005-06-09 Maithreyan Srinivasan Novel sulfurylase-luciferase fusion proteins and thermostable sulfurylase
JP2005509426A (ja) * 2001-11-14 2005-04-14 イギリス国 細胞内ａｔｐおよび／または遺伝子発現の測定方法
KR101076102B1 (ko) 2002-02-21 2011-10-21 비로노바티브 비.브이. 메타뉴모바이러스에서 유래한 이종성 항원을 포함하는재조합 파라인플루엔자 바이러스 발현 시스템 및 백신
JP4634371B2 (ja) 2003-03-27 2011-02-16 ピーティーシー セラピューティクス，インコーポレーテッド ｔＲＮＡスプライシングエンドヌクレアーゼに標的化する化合物の同定方法、および該化合物の抗増殖剤としての使用
WO2004087884A2 (en) 2003-03-27 2004-10-14 Ptc Therapeutics, Inc TARGETING ENZYMES OF THE tRNA SPLICING PATHWAY FOR IDENTIFICATION OF ANTI-FUNGAL AND/OR ANTI-PROLIFERATIVE MOLECULES
GB2400659B8 (en) 2003-04-17 2007-07-09 Cambrex Bio Science Nottingham Assay methods and materials
US7494781B1 (en) 2003-05-12 2009-02-24 Charm Sciences, Inc. Sensitive method for detecting low levels of ATP
EP1687399A4 (en) * 2003-10-03 2007-09-05 Univ Michigan Tech MODIFIED LUCIFERASE
US7728118B2 (en) 2004-09-17 2010-06-01 Promega Corporation Synthetic nucleic acid molecule compositions and methods of preparation
US20060234323A1 (en) * 2005-04-18 2006-10-19 Cali James J Luminescent ATP detection with extended linearity
US8227572B2 (en) * 2005-08-19 2012-07-24 Commonwealth Scientific And Industrial Research Organisation Arachnocampa luciferases
WO2008079228A1 (en) * 2006-12-20 2008-07-03 Danisco Us, Inc., Genencor Division Assays for improved fungal strains
JP5188771B2 (ja) 2007-09-26 2013-04-24 株式会社日立製作所 変異型ホタルルシフェラーゼ
WO2009075306A1 (ja) 2007-12-10 2009-06-18 Olympus Corporation 発光測定方法および発光測定システム
JP5753083B2 (ja) 2008-07-22 2015-07-22 プロメガ コーポレイションＰｒｏｍｅｇａ Ｃｏｒｐｏｒａｔｉｏｎ Ａｄｐ検出に基づく発光ホスホトランスフェラーゼまたはａｔｐヒドロラーゼのアッセイ法
JP2010081908A (ja) * 2008-10-02 2010-04-15 Hitachi Ltd 変異型のホタルルシフェラーゼ
JP2011120559A (ja) 2008-12-26 2011-06-23 Kikkoman Corp ホタルルシフェラーゼ、その遺伝子、およびホタルルシフェラーゼの製造法
PT2635583E (pt) 2010-11-02 2015-09-11 Promega Corp Derivados da coelenterazina e métodos de utilização da mesma
US8669103B2 (en) 2010-11-02 2014-03-11 Promega Corporation Oplophorus-derived luciferases, novel coelenterazine substrates, and methods of use
JP5875762B2 (ja) * 2010-12-17 2016-03-02 オリンパス株式会社 甲虫類ルシフェラーゼ
CN111004835B (zh) 2013-02-22 2023-12-29 普洛麦格公司 用于荧光素酶和核苷磷酸的发光检测的稳定制剂
JP6071634B2 (ja) * 2013-02-25 2017-02-01 オリンパス株式会社 Ａｔｐの定量方法及びそれに用いるキット
US9927430B2 (en) 2014-01-29 2018-03-27 Promega Corporation Pro-substrates for live cell applications
WO2015116867A1 (en) 2014-01-29 2015-08-06 Promega Corporation Quinone-masked probes as labeling reagents for cell uptake measurements
JP6412718B2 (ja) * 2014-05-27 2018-10-24 オリンパス株式会社 プロモーターアッセイ
WO2016040788A1 (en) 2014-09-11 2016-03-17 Promega Corporation Luciferase sequences utilizing infrared-emitting substrates to produce enhanced luminescence
US9677117B2 (en) 2014-10-08 2017-06-13 Promega Corporation Bioluminescent succinate detection assay
JP6493316B2 (ja) 2016-06-21 2019-04-03 東亜ディーケーケー株式会社 変異型甲虫ルシフェラーゼ、遺伝子、組換えベクター、形質転換体、及び変異型甲虫ルシフェラーゼの製造方法
US11261478B2 (en) * 2016-10-13 2022-03-01 Promega Corporation Thermal stable luciferase with improved resistance to inhibition by luciferin break-down products
WO2021162123A1 (ja) 2020-02-14 2021-08-19 キッコーマン株式会社 試料中のａｔｐ、並びにａｍｐ及び／又はａｄｐを測定するための液体組成物
EP4560025A1 (en) 2022-07-22 2025-05-28 Kikkoman Corporation Method and kit for detecting microorganism or cell, or detecting microorganism related substance or cell related substance
CN120457210A (zh) 2023-01-27 2025-08-08 龟甲万株式会社 萤光素酶突变体

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US5196524A (en) * 1989-01-06 1993-03-23 Eli Lilly And Company Fusion reporter gene for bacterial luciferase
US5219737A (en) 1990-03-27 1993-06-15 Kikkoman Corporation Mutant luciferase of a firefly, mutant luciferase genes, recombinant dnas containing the genes and a method of producing mutant luciferase
ES2301160T3 (es) * 1994-01-03 2008-06-16 Promega Corporation Luciferasas mutantes.
GB9501170D0 (en) 1994-03-23 1995-03-08 Secr Defence Luciferases
AU707243B2 (en) * 1995-01-20 1999-07-08 Promega Corporation Mutant luciferases

Also Published As

Publication number Publication date
EP0973874A2 (en) 2000-01-26
DE69811876D1 (de) 2003-04-10
AU731408B2 (en) 2001-03-29
JP4302777B2 (ja) 2009-07-29
US6265177B1 (en) 2001-07-24
ES2194309T3 (es) 2003-11-16
WO1998046729A2 (en) 1998-10-22
PT973874E (pt) 2003-07-31
DE69811876T2 (de) 2003-12-04
CA2283287C (en) 2009-12-08
ATE233810T1 (de) 2003-03-15
GB9707486D0 (en) 1997-05-28
NZ337373A (en) 2001-04-27
AU6929398A (en) 1998-11-11
CA2283287A1 (en) 1998-10-22
EP0973874B1 (en) 2003-03-05
WO1998046729A3 (en) 1999-01-28
JP2001518799A (ja) 2001-10-16

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