DK0977873T3 - Fremgangsmåde til spaltning af fusionsproteiner - Google Patents

Fremgangsmåde til spaltning af fusionsproteiner

Info

Publication number
DK0977873T3
DK0977873T3 DK98916746T DK98916746T DK0977873T3 DK 0977873 T3 DK0977873 T3 DK 0977873T3 DK 98916746 T DK98916746 T DK 98916746T DK 98916746 T DK98916746 T DK 98916746T DK 0977873 T3 DK0977873 T3 DK 0977873T3
Authority
DK
Denmark
Prior art keywords
cleavage
fusion proteins
fusion
proteins
Prior art date
Application number
DK98916746T
Other languages
English (en)
Inventor
Rooijen Gijs Van
Maurice Moloney
Joenel Alcantara
Original Assignee
Sembiosys Genetics Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Sembiosys Genetics Inc filed Critical Sembiosys Genetics Inc
Application granted granted Critical
Publication of DK0977873T3 publication Critical patent/DK0977873T3/da

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23L—FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
    • A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
    • A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
    • A23L33/17—Amino acids, peptides or proteins
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P3/00—Drugs for disorders of the metabolism
    • A61P3/02—Nutrients, e.g. vitamins, minerals
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/61—Growth hormone [GH], i.e. somatotropin
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/81—Protease inhibitors
    • C07K14/815—Protease inhibitors from leeches, e.g. hirudin, eglin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/20—Fusion polypeptide containing a tag with affinity for a non-protein ligand
    • C07K2319/21—Fusion polypeptide containing a tag with affinity for a non-protein ligand containing a His-tag
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/50—Fusion polypeptide containing protease site
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/70—Fusion polypeptide containing domain for protein-protein interaction
    • C07K2319/74—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor
    • C07K2319/75—Fusion polypeptide containing domain for protein-protein interaction containing a fusion for binding to a cell surface receptor containing a fusion for activation of a cell surface receptor, e.g. thrombopoeitin, NPY and other peptide hormones

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Wood Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Endocrinology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Plant Pathology (AREA)
  • Nutrition Science (AREA)
  • Animal Behavior & Ethology (AREA)
  • Toxicology (AREA)
  • Public Health (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Mycology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Food Science & Technology (AREA)
  • Polymers & Plastics (AREA)
  • General Chemical & Material Sciences (AREA)
  • Veterinary Medicine (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Hematology (AREA)
  • Obesity (AREA)
  • Diabetes (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
DK98916746T 1997-04-25 1998-04-23 Fremgangsmåde til spaltning af fusionsproteiner DK0977873T3 (da)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US4425497P 1997-04-25 1997-04-25
PCT/CA1998/000398 WO1998049326A1 (en) 1997-04-25 1998-04-23 Method for cleavage of fusion proteins

Publications (1)

Publication Number Publication Date
DK0977873T3 true DK0977873T3 (da) 2007-02-05

Family

ID=21931348

Family Applications (1)

Application Number Title Priority Date Filing Date
DK98916746T DK0977873T3 (da) 1997-04-25 1998-04-23 Fremgangsmåde til spaltning af fusionsproteiner

Country Status (18)

Country Link
US (1) US7501265B1 (da)
EP (1) EP0977873B1 (da)
JP (1) JP4120972B2 (da)
KR (2) KR100635623B1 (da)
AR (1) AR012499A1 (da)
AT (1) ATE341633T1 (da)
AU (1) AU742794B2 (da)
BR (1) BR9809416A (da)
CA (1) CA2286861A1 (da)
DE (1) DE69836075T2 (da)
DK (1) DK0977873T3 (da)
ES (1) ES2273414T3 (da)
IL (2) IL132259A0 (da)
MX (1) MX219629B (da)
NZ (1) NZ500736A (da)
TW (1) TW505697B (da)
WO (1) WO1998049326A1 (da)
ZA (1) ZA983471B (da)

Families Citing this family (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2372907A1 (en) * 1999-04-30 2000-11-09 Andrew Darrow Zymogen activation system
US6420157B1 (en) * 1999-04-30 2002-07-16 Ortho-Mcneil Pharmaceutical, Inc. Zymogen activation system
CZ302366B6 (cs) * 1999-08-09 2011-04-13 Sandoz Ag Zpusob rekombinantní produkce heterologního polypeptidu pomocí peptidu s autoproteolytickou aktivitou
HK1047127B (en) * 1999-08-09 2015-08-21 Sandoz Ag Production of proteins by autoproteolytic cleavage
CA2381438C (en) * 1999-08-23 2010-10-05 Sembiosys Genetics Inc. Commercial production of chymosin in plants
US7390936B1 (en) 1999-08-23 2008-06-24 Sembiosys Genetics Inc. Commercial production of chymosin in plants
US6750034B1 (en) 2000-06-30 2004-06-15 Ortho-Mcneil Pharmaceutical, Inc. DNA encoding human serine protease D-G
US6737262B1 (en) 2000-07-11 2004-05-18 Robert I. Bolla Animal feed containing polypeptides
ATE405660T1 (de) 2003-06-17 2008-09-15 Sembiosys Genetics Inc Verfahren zur insulinproduktion in pflanzen
EP1651751B1 (en) 2003-08-06 2015-11-18 University Of Maryland Biotechnology Institute Engineered proteases for affinity purification and processing of fusion proteins
CN102389575B (zh) * 2011-11-18 2013-01-23 武汉凯肽来生物科技有限公司 一种基因工程口服dna疫苗及制备方法和应用
WO2013142859A2 (en) * 2012-03-23 2013-09-26 Wuhan Peptech Pharmaceutical Co., Ltd. Fusion proteins of superfolder green fluorescent protein and use thereof
US10947552B1 (en) 2020-09-30 2021-03-16 Alpine Roads, Inc. Recombinant fusion proteins for producing milk proteins in plants
IL301396A (en) 2020-09-30 2023-05-01 Nobell Foods Inc Recombinant milk proteins and food compositions comprising the same
US10894812B1 (en) 2020-09-30 2021-01-19 Alpine Roads, Inc. Recombinant milk proteins

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4180559A (en) * 1978-01-05 1979-12-25 Richardson-Merrell Inc. Coated 1-(2-chlorodibenzo[b,f]oxepin-10-yl)-4-methylpiperazine compositions
US4774183A (en) * 1983-06-29 1988-09-27 Kansas State University Research Foundation Process for producing fructose
AU3036684A (en) 1983-07-07 1985-01-10 Genex Corp. Production of bovine calf chymosin
US4743679A (en) 1986-02-24 1988-05-10 Creative Biomolecules, Inc. Process for producing human epidermal growth factor and analogs thereof
WO1991011454A1 (en) 1990-01-24 1991-08-08 The Upjohn Company Method of purifying recombinant polypeptides
US5650554A (en) * 1991-02-22 1997-07-22 Sembiosys Genetics Inc. Oil-body proteins as carriers of high-value peptides in plants
EP0679189B1 (en) * 1993-01-15 2003-06-04 Genetics Institute, LLC Cloning of enterokinase and method of use
US6265204B1 (en) * 1997-01-17 2001-07-24 Genencor International, Inc. DNA sequences, vectors, and fusion polypeptides for secretion of polypeptides in filamentous fungi

Also Published As

Publication number Publication date
KR20010006506A (ko) 2001-01-26
EP0977873B1 (en) 2006-10-04
ES2273414T3 (es) 2007-05-01
ZA983471B (en) 1998-10-27
DE69836075D1 (de) 2006-11-16
KR100635623B1 (ko) 2006-10-18
IL132259A (en) 2007-05-15
WO1998049326A1 (en) 1998-11-05
KR20050100712A (ko) 2005-10-19
BR9809416A (pt) 2000-06-13
AU742794B2 (en) 2002-01-10
CA2286861A1 (en) 1998-11-05
NZ500736A (en) 2005-01-28
KR100609886B1 (ko) 2006-08-18
DE69836075T2 (de) 2007-06-06
AU7024098A (en) 1998-11-24
IL132259A0 (en) 2001-03-19
US7501265B1 (en) 2009-03-10
ATE341633T1 (de) 2006-10-15
MX219629B (es) 2004-03-30
TW505697B (en) 2002-10-11
EP0977873A1 (en) 2000-02-09
AR012499A1 (es) 2000-10-18
JP2001527400A (ja) 2001-12-25
MX9909801A (es) 2000-03-31
JP4120972B2 (ja) 2008-07-16

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