DK0981609T3 - Fremgangsmåde til kloning af mRNA'er og display af differentielt udtrykte transkripter (DODET) - Google Patents

Fremgangsmåde til kloning af mRNA'er og display af differentielt udtrykte transkripter (DODET)

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Publication number
DK0981609T3
DK0981609T3 DK98919092T DK98919092T DK0981609T3 DK 0981609 T3 DK0981609 T3 DK 0981609T3 DK 98919092 T DK98919092 T DK 98919092T DK 98919092 T DK98919092 T DK 98919092T DK 0981609 T3 DK0981609 T3 DK 0981609T3
Authority
DK
Denmark
Prior art keywords
dodet
mrnas
cloning
differentially expressed
expressed transcripts
Prior art date
Application number
DK98919092T
Other languages
English (en)
Inventor
Peter Rolf Warthoe
Original Assignee
Azign Bioscience As
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Azign Bioscience As filed Critical Azign Bioscience As
Application granted granted Critical
Publication of DK0981609T3 publication Critical patent/DK0981609T3/da

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1096Processes for the isolation, preparation or purification of DNA or RNA cDNA Synthesis; Subtracted cDNA library construction, e.g. RT, RT-PCR
    • GPHYSICS
    • G16INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16BBIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
    • G16B35/00ICT specially adapted for in silico combinatorial libraries of nucleic acids, proteins or peptides
    • G16B35/20Screening of libraries
    • GPHYSICS
    • G16INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16BBIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
    • G16B25/00ICT specially adapted for hybridisation; ICT specially adapted for gene or protein expression
    • G16B25/10Gene or protein expression profiling; Expression-ratio estimation or normalisation
    • GPHYSICS
    • G16INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16BBIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
    • G16B35/00ICT specially adapted for in silico combinatorial libraries of nucleic acids, proteins or peptides
    • GPHYSICS
    • G16INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16CCOMPUTATIONAL CHEMISTRY; CHEMOINFORMATICS; COMPUTATIONAL MATERIALS SCIENCE
    • G16C20/00Chemoinformatics, i.e. ICT specially adapted for the handling of physicochemical or structural data of chemical particles, elements, compounds or mixtures
    • G16C20/60In silico combinatorial chemistry
    • GPHYSICS
    • G16INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16BBIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
    • G16B25/00ICT specially adapted for hybridisation; ICT specially adapted for gene or protein expression

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Physics & Mathematics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Biotechnology (AREA)
  • Theoretical Computer Science (AREA)
  • Biophysics (AREA)
  • Molecular Biology (AREA)
  • Medical Informatics (AREA)
  • Evolutionary Biology (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Library & Information Science (AREA)
  • Biochemistry (AREA)
  • Zoology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Computing Systems (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
DK98919092T 1997-05-13 1998-05-13 Fremgangsmåde til kloning af mRNA'er og display af differentielt udtrykte transkripter (DODET) DK0981609T3 (da)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
DK54797 1997-05-13
US87103097A 1997-05-19 1997-05-19
DK43298 1998-03-27
PCT/DK1998/000186 WO1998051789A2 (en) 1997-05-13 1998-05-13 A METHOD TO CLONE mRNAs AND DISPLAY OF DIFFERENTIALLY EXPRESSED TRANSCRIPTS (DODET)

Publications (1)

Publication Number Publication Date
DK0981609T3 true DK0981609T3 (da) 2001-11-12

Family

ID=27220672

Family Applications (1)

Application Number Title Priority Date Filing Date
DK98919092T DK0981609T3 (da) 1997-05-13 1998-05-13 Fremgangsmåde til kloning af mRNA'er og display af differentielt udtrykte transkripter (DODET)

Country Status (9)

Country Link
EP (2) EP1138764A3 (da)
JP (1) JP2001515362A (da)
AT (1) ATE205879T1 (da)
AU (1) AU7206498A (da)
DE (1) DE69801749T2 (da)
DK (1) DK0981609T3 (da)
ES (1) ES2163271T3 (da)
PT (1) PT981609E (da)
WO (1) WO1998051789A2 (da)

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US6989259B2 (en) 1997-04-22 2006-01-24 Invitrogen Corporation Recombinant methods for making reverse transcriptases and mutants thereof
US20020090612A1 (en) * 1999-01-08 2002-07-11 Jonathan M. Rothberg Method of identifying nucleic acids
SG89289A1 (en) * 1999-08-14 2002-06-18 Kent Ridge Digital Labs Classification by aggregating emerging patterns
US6468749B1 (en) 2000-03-30 2002-10-22 Quark Biotech, Inc. Sequence-dependent gene sorting techniques
US6387624B1 (en) 2000-04-14 2002-05-14 Incyte Pharmaceuticals, Inc. Construction of uni-directionally cloned cDNA libraries from messenger RNA for improved 3′ end DNA sequencing
US7300751B2 (en) 2000-06-30 2007-11-27 Syngenta Participations Ag Method for identification of genetic markers
WO2002002805A2 (en) 2000-06-30 2002-01-10 Syngenta Participations Ag Method for identification, separation and quantitative measurement of nucleic acid fragments
US6706476B1 (en) * 2000-08-22 2004-03-16 Azign Bioscience A/S Process for amplifying and labeling single stranded cDNA by 5′ ligated adaptor mediated amplification
JP4340779B2 (ja) 2000-10-05 2009-10-07 独立行政法人理化学研究所 可変突出部位を含むオリゴヌクレオチドリンカー及び前記リンカーを用いたポリヌクレオチドライブラリーの調製方法
AU2261802A (en) * 2000-12-12 2002-06-24 Masumi Abe Method of analyzing gene expression
US6727068B2 (en) 2001-01-24 2004-04-27 Syngenta Participations Ag Method for non-redundant library construction
EP1434883A2 (en) * 2001-10-02 2004-07-07 Azign Bioscience A/S Specific differential display arrays
CA2496517A1 (en) * 2002-09-05 2004-03-18 Plant Bioscience Limited Genome partitioning
CA2513899C (en) * 2003-01-29 2013-03-26 454 Corporation Methods of amplifying and sequencing nucleic acids
US7498135B2 (en) 2003-06-19 2009-03-03 National Institute Of Radiological Sciences Method for preparing gene expression profile
JP4217780B2 (ja) * 2003-06-19 2009-02-04 独立行政法人放射線医学総合研究所 遺伝子発現プロファイルの作製方法
US7575863B2 (en) 2004-05-28 2009-08-18 Applied Biosystems, Llc Methods, compositions, and kits comprising linker probes for quantifying polynucleotides
JP4879975B2 (ja) * 2005-05-31 2012-02-22 アプライド バイオシステムズ リミテッド ライアビリティー カンパニー 短い核酸のマルチプレックス増幅
DE602006013831D1 (de) * 2005-06-23 2010-06-02 Keygene Nv Verbesserte strategien zur sequenzierung komplexer genome unter verwendung von sequenziertechniken mit hohem durchsatz
DK2302070T3 (da) 2005-06-23 2012-11-26 Keygene Nv Strategier til identifikation og detektion af polymorfismer med højt gennemløb
EP1924713B1 (en) 2005-08-24 2011-11-09 Life Technologies Corporation A method to quantify sirnas, mirnas and polymorphic mirnas
US10316364B2 (en) 2005-09-29 2019-06-11 Keygene N.V. Method for identifying the source of an amplicon
WO2007037678A2 (en) 2005-09-29 2007-04-05 Keygene N.V. High throughput screening of mutagenized populations
CN103937899B (zh) 2005-12-22 2017-09-08 凯津公司 用于基于aflp的高通量多态性检测的方法
EP3239304B1 (en) 2006-04-04 2020-08-19 Keygene N.V. High throughput detection of molecular markers based on aflp and high troughput sequencing
EP2248914A1 (en) * 2009-05-05 2010-11-10 Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V. The use of class IIB restriction endonucleases in 2nd generation sequencing applications
US10385392B2 (en) 2011-12-30 2019-08-20 Abbott Molecular Inc. Nucleic acid hybridization probes

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1991009944A2 (en) * 1989-12-22 1991-07-11 F.Hoffmann-La Roche Ag High temperature reverse transcriptases
JP2843675B2 (ja) * 1992-03-11 1999-01-06 ダナ−ファーバー・キャンサー・インスチチュート・インコーポレーテッド メッセンジャーrnaの同定、単離およびクローニング
US5482845A (en) * 1993-09-24 1996-01-09 The Trustees Of Columbia University In The City Of New York Method for construction of normalized cDNA libraries
US5459037A (en) * 1993-11-12 1995-10-17 The Scripps Research Institute Method for simultaneous identification of differentially expressed mRNAs and measurement of relative concentrations
DE19518505A1 (de) * 1995-05-19 1996-11-21 Max Planck Gesellschaft Verfahren zur Genexpressionsanalyse
US5712126A (en) * 1995-08-01 1998-01-27 Yale University Analysis of gene expression by display of 3-end restriction fragments of CDNA
AU2264197A (en) * 1996-02-09 1997-08-28 Government Of The United States Of America, As Represented By The Secretary Of The Department Of Health And Human Services, The Restriction display (rd-pcr) of differentially expressed mrnas
US5830714A (en) * 1996-04-17 1998-11-03 Molecular Biology Resources, Inc. Biologically active fragment of bacillus stearothermophilus DNA polymerase

Also Published As

Publication number Publication date
ES2163271T3 (es) 2002-01-16
WO1998051789A3 (en) 1999-03-18
EP0981609A2 (en) 2000-03-01
AU7206498A (en) 1998-12-08
DE69801749D1 (de) 2001-10-25
EP1138764A2 (en) 2001-10-04
WO1998051789A2 (en) 1998-11-19
WO1998051789A9 (en) 1999-12-09
PT981609E (pt) 2002-03-28
JP2001515362A (ja) 2001-09-18
ATE205879T1 (de) 2001-10-15
DE69801749T2 (de) 2002-07-04
EP1138764A3 (en) 2001-10-24
EP0981609B1 (en) 2001-09-19

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