ATE205879T1 - Eine methode zum klonieren von mrna und darstellung von differentiell exprimierten transcripten (dodet) - Google Patents
Eine methode zum klonieren von mrna und darstellung von differentiell exprimierten transcripten (dodet)Info
- Publication number
- ATE205879T1 ATE205879T1 AT98919092T AT98919092T ATE205879T1 AT E205879 T1 ATE205879 T1 AT E205879T1 AT 98919092 T AT98919092 T AT 98919092T AT 98919092 T AT98919092 T AT 98919092T AT E205879 T1 ATE205879 T1 AT E205879T1
- Authority
- AT
- Austria
- Prior art keywords
- dodet
- differentially expressed
- expressed transcripts
- reverse transcriptase
- displaying
- Prior art date
Links
- 238000010367 cloning Methods 0.000 title 1
- 238000000034 method Methods 0.000 title 1
- 102100034343 Integrase Human genes 0.000 abstract 3
- 108010092799 RNA-directed DNA polymerase Proteins 0.000 abstract 3
- 108020004635 Complementary DNA Proteins 0.000 abstract 1
- 108020004414 DNA Proteins 0.000 abstract 1
- 108090000623 proteins and genes Proteins 0.000 abstract 1
- 238000010839 reverse transcription Methods 0.000 abstract 1
Classifications
-
- G—PHYSICS
- G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
- G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
- G16B35/00—ICT specially adapted for in silico combinatorial libraries of nucleic acids, proteins or peptides
- G16B35/20—Screening of libraries
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1096—Processes for the isolation, preparation or purification of DNA or RNA cDNA Synthesis; Subtracted cDNA library construction, e.g. RT, RT-PCR
-
- G—PHYSICS
- G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
- G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
- G16B25/00—ICT specially adapted for hybridisation; ICT specially adapted for gene or protein expression
- G16B25/10—Gene or protein expression profiling; Expression-ratio estimation or normalisation
-
- G—PHYSICS
- G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
- G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
- G16B35/00—ICT specially adapted for in silico combinatorial libraries of nucleic acids, proteins or peptides
-
- G—PHYSICS
- G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
- G16C—COMPUTATIONAL CHEMISTRY; CHEMOINFORMATICS; COMPUTATIONAL MATERIALS SCIENCE
- G16C20/00—Chemoinformatics, i.e. ICT specially adapted for the handling of physicochemical or structural data of chemical particles, elements, compounds or mixtures
- G16C20/60—In silico combinatorial chemistry
-
- G—PHYSICS
- G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
- G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
- G16B25/00—ICT specially adapted for hybridisation; ICT specially adapted for gene or protein expression
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Physics & Mathematics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Chemical & Material Sciences (AREA)
- Genetics & Genomics (AREA)
- General Health & Medical Sciences (AREA)
- Bioinformatics & Computational Biology (AREA)
- Biotechnology (AREA)
- Theoretical Computer Science (AREA)
- Biophysics (AREA)
- Molecular Biology (AREA)
- Medical Informatics (AREA)
- Evolutionary Biology (AREA)
- Spectroscopy & Molecular Physics (AREA)
- Library & Information Science (AREA)
- Biochemistry (AREA)
- Zoology (AREA)
- Crystallography & Structural Chemistry (AREA)
- Biomedical Technology (AREA)
- General Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Organic Chemistry (AREA)
- Computing Systems (AREA)
- Plant Pathology (AREA)
- Microbiology (AREA)
- Medicinal Chemistry (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Enzymes And Modification Thereof (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Applications Claiming Priority (4)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DK54797 | 1997-05-13 | ||
| US87103097A | 1997-05-19 | 1997-05-19 | |
| DK43298 | 1998-03-27 | ||
| PCT/DK1998/000186 WO1998051789A2 (en) | 1997-05-13 | 1998-05-13 | A METHOD TO CLONE mRNAs AND DISPLAY OF DIFFERENTIALLY EXPRESSED TRANSCRIPTS (DODET) |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| ATE205879T1 true ATE205879T1 (de) | 2001-10-15 |
Family
ID=27220672
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| AT98919092T ATE205879T1 (de) | 1997-05-13 | 1998-05-13 | Eine methode zum klonieren von mrna und darstellung von differentiell exprimierten transcripten (dodet) |
Country Status (9)
| Country | Link |
|---|---|
| EP (2) | EP1138764A3 (de) |
| JP (1) | JP2001515362A (de) |
| AT (1) | ATE205879T1 (de) |
| AU (1) | AU7206498A (de) |
| DE (1) | DE69801749T2 (de) |
| DK (1) | DK0981609T3 (de) |
| ES (1) | ES2163271T3 (de) |
| PT (1) | PT981609E (de) |
| WO (1) | WO1998051789A2 (de) |
Families Citing this family (27)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6989259B2 (en) | 1997-04-22 | 2006-01-24 | Invitrogen Corporation | Recombinant methods for making reverse transcriptases and mutants thereof |
| US20020090612A1 (en) * | 1999-01-08 | 2002-07-11 | Jonathan M. Rothberg | Method of identifying nucleic acids |
| SG89289A1 (en) * | 1999-08-14 | 2002-06-18 | Kent Ridge Digital Labs | Classification by aggregating emerging patterns |
| US6468749B1 (en) | 2000-03-30 | 2002-10-22 | Quark Biotech, Inc. | Sequence-dependent gene sorting techniques |
| US6387624B1 (en) | 2000-04-14 | 2002-05-14 | Incyte Pharmaceuticals, Inc. | Construction of uni-directionally cloned cDNA libraries from messenger RNA for improved 3′ end DNA sequencing |
| US7300751B2 (en) | 2000-06-30 | 2007-11-27 | Syngenta Participations Ag | Method for identification of genetic markers |
| WO2002002805A2 (en) | 2000-06-30 | 2002-01-10 | Syngenta Participations Ag | Method for identification, separation and quantitative measurement of nucleic acid fragments |
| US6706476B1 (en) * | 2000-08-22 | 2004-03-16 | Azign Bioscience A/S | Process for amplifying and labeling single stranded cDNA by 5′ ligated adaptor mediated amplification |
| JP4340779B2 (ja) | 2000-10-05 | 2009-10-07 | 独立行政法人理化学研究所 | 可変突出部位を含むオリゴヌクレオチドリンカー及び前記リンカーを用いたポリヌクレオチドライブラリーの調製方法 |
| AU2261802A (en) * | 2000-12-12 | 2002-06-24 | Masumi Abe | Method of analyzing gene expression |
| US6727068B2 (en) | 2001-01-24 | 2004-04-27 | Syngenta Participations Ag | Method for non-redundant library construction |
| EP1434883A2 (de) * | 2001-10-02 | 2004-07-07 | Azign Bioscience A/S | Arrays mit spezifischer differentieller präsentation |
| CA2496517A1 (en) * | 2002-09-05 | 2004-03-18 | Plant Bioscience Limited | Genome partitioning |
| CA2513899C (en) * | 2003-01-29 | 2013-03-26 | 454 Corporation | Methods of amplifying and sequencing nucleic acids |
| US7498135B2 (en) | 2003-06-19 | 2009-03-03 | National Institute Of Radiological Sciences | Method for preparing gene expression profile |
| JP4217780B2 (ja) * | 2003-06-19 | 2009-02-04 | 独立行政法人放射線医学総合研究所 | 遺伝子発現プロファイルの作製方法 |
| US7575863B2 (en) | 2004-05-28 | 2009-08-18 | Applied Biosystems, Llc | Methods, compositions, and kits comprising linker probes for quantifying polynucleotides |
| JP4879975B2 (ja) * | 2005-05-31 | 2012-02-22 | アプライド バイオシステムズ リミテッド ライアビリティー カンパニー | 短い核酸のマルチプレックス増幅 |
| DE602006013831D1 (de) * | 2005-06-23 | 2010-06-02 | Keygene Nv | Verbesserte strategien zur sequenzierung komplexer genome unter verwendung von sequenziertechniken mit hohem durchsatz |
| DK2302070T3 (da) | 2005-06-23 | 2012-11-26 | Keygene Nv | Strategier til identifikation og detektion af polymorfismer med højt gennemløb |
| EP1924713B1 (de) | 2005-08-24 | 2011-11-09 | Life Technologies Corporation | Verfahren zur quantifizierung von sirnas, mirnas und polymorphen mirnas |
| US10316364B2 (en) | 2005-09-29 | 2019-06-11 | Keygene N.V. | Method for identifying the source of an amplicon |
| WO2007037678A2 (en) | 2005-09-29 | 2007-04-05 | Keygene N.V. | High throughput screening of mutagenized populations |
| CN103937899B (zh) | 2005-12-22 | 2017-09-08 | 凯津公司 | 用于基于aflp的高通量多态性检测的方法 |
| EP3239304B1 (de) | 2006-04-04 | 2020-08-19 | Keygene N.V. | Nachweis von molekularmarkern mit hohem durchsatz auf der basis von aflp und mit hoher durchsatzsequenzierung |
| EP2248914A1 (de) * | 2009-05-05 | 2010-11-10 | Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V. | Die Verwendung von IIB-Restriktionsendonukleasen in Sequenzierungsanwendungen der zweiten Generation |
| US10385392B2 (en) | 2011-12-30 | 2019-08-20 | Abbott Molecular Inc. | Nucleic acid hybridization probes |
Family Cites Families (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1991009944A2 (en) * | 1989-12-22 | 1991-07-11 | F.Hoffmann-La Roche Ag | High temperature reverse transcriptases |
| JP2843675B2 (ja) * | 1992-03-11 | 1999-01-06 | ダナ−ファーバー・キャンサー・インスチチュート・インコーポレーテッド | メッセンジャーｒｎａの同定、単離およびクローニング |
| US5482845A (en) * | 1993-09-24 | 1996-01-09 | The Trustees Of Columbia University In The City Of New York | Method for construction of normalized cDNA libraries |
| US5459037A (en) * | 1993-11-12 | 1995-10-17 | The Scripps Research Institute | Method for simultaneous identification of differentially expressed mRNAs and measurement of relative concentrations |
| DE19518505A1 (de) * | 1995-05-19 | 1996-11-21 | Max Planck Gesellschaft | Verfahren zur Genexpressionsanalyse |
| US5712126A (en) * | 1995-08-01 | 1998-01-27 | Yale University | Analysis of gene expression by display of 3-end restriction fragments of CDNA |
| AU2264197A (en) * | 1996-02-09 | 1997-08-28 | Government Of The United States Of America, As Represented By The Secretary Of The Department Of Health And Human Services, The | Restriction display (rd-pcr) of differentially expressed mrnas |
| US5830714A (en) * | 1996-04-17 | 1998-11-03 | Molecular Biology Resources, Inc. | Biologically active fragment of bacillus stearothermophilus DNA polymerase |
-
1998
- 1998-05-13 JP JP54871398A patent/JP2001515362A/ja not_active Ceased
- 1998-05-13 EP EP01202318A patent/EP1138764A3/de not_active Withdrawn
- 1998-05-13 AT AT98919092T patent/ATE205879T1/de not_active IP Right Cessation
- 1998-05-13 ES ES98919092T patent/ES2163271T3/es not_active Expired - Lifetime
- 1998-05-13 WO PCT/DK1998/000186 patent/WO1998051789A2/en not_active Ceased
- 1998-05-13 DK DK98919092T patent/DK0981609T3/da active
- 1998-05-13 AU AU72064/98A patent/AU7206498A/en not_active Abandoned
- 1998-05-13 PT PT98919092T patent/PT981609E/pt unknown
- 1998-05-13 EP EP98919092A patent/EP0981609B1/de not_active Expired - Lifetime
- 1998-05-13 DE DE69801749T patent/DE69801749T2/de not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| ES2163271T3 (es) | 2002-01-16 |
| WO1998051789A3 (en) | 1999-03-18 |
| EP0981609A2 (de) | 2000-03-01 |
| AU7206498A (en) | 1998-12-08 |
| DE69801749D1 (de) | 2001-10-25 |
| DK0981609T3 (da) | 2001-11-12 |
| EP1138764A2 (de) | 2001-10-04 |
| WO1998051789A2 (en) | 1998-11-19 |
| WO1998051789A9 (en) | 1999-12-09 |
| PT981609E (pt) | 2002-03-28 |
| JP2001515362A (ja) | 2001-09-18 |
| DE69801749T2 (de) | 2002-07-04 |
| EP1138764A3 (de) | 2001-10-24 |
| EP0981609B1 (de) | 2001-09-19 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| UEP | Publication of translation of european patent specification | ||
| REN | Ceased due to non-payment of the annual fee |