DK1032680T3 - En hidtil ukendt metode til integration af fremmed DNA ind i eukaryote genomer - Google Patents

En hidtil ukendt metode til integration af fremmed DNA ind i eukaryote genomer

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Publication number
DK1032680T3
DK1032680T3 DK98960261T DK98960261T DK1032680T3 DK 1032680 T3 DK1032680 T3 DK 1032680T3 DK 98960261 T DK98960261 T DK 98960261T DK 98960261 T DK98960261 T DK 98960261T DK 1032680 T3 DK1032680 T3 DK 1032680T3
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DK
Denmark
Prior art keywords
methods
site
dna
target sites
compositions
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DK98960261T
Other languages
Danish (da)
English (en)
Inventor
Christopher L Baszczynski
William J Gordon-Kamm
Leszek Alexander Lyznik
Laura A Tagliani
Xueni Guan
Aragula Gururaj Rao
Original Assignee
Pioneer Hi Bred Int
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Publication of DK1032680T3 publication Critical patent/DK1032680T3/da

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8202—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by biological means, e.g. cell mediated or natural vector
    • C12N15/8203—Virus mediated transformation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8202—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation by biological means, e.g. cell mediated or natural vector
    • C12N15/8205—Agrobacterium mediated transformation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8213—Targeted insertion of genes into the plant genome by homologous recombination
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Cell Biology (AREA)
  • Virology (AREA)
  • Mycology (AREA)
  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Saccharide Compounds (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
DK98960261T 1997-11-18 1998-11-17 En hidtil ukendt metode til integration af fremmed DNA ind i eukaryote genomer DK1032680T3 (da)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US6561397P 1997-11-18 1997-11-18
US6562797P 1997-11-18 1997-11-18
US9943598P 1998-09-08 1998-09-08

Publications (1)

Publication Number Publication Date
DK1032680T3 true DK1032680T3 (da) 2006-05-22

Family

ID=27370819

Family Applications (1)

Application Number Title Priority Date Filing Date
DK98960261T DK1032680T3 (da) 1997-11-18 1998-11-17 En hidtil ukendt metode til integration af fremmed DNA ind i eukaryote genomer

Country Status (12)

Country Link
US (3) US6262341B1 (fr)
EP (1) EP1032680B1 (fr)
AT (1) ATE317440T1 (fr)
AU (1) AU745960C (fr)
BR (1) BR9814669A (fr)
CA (1) CA2306188C (fr)
DE (1) DE69833457T2 (fr)
DK (1) DK1032680T3 (fr)
ES (1) ES2256972T3 (fr)
NZ (1) NZ504511A (fr)
PT (1) PT1032680E (fr)
WO (1) WO1999025840A1 (fr)

Families Citing this family (242)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6964861B1 (en) 1998-11-13 2005-11-15 Invitrogen Corporation Enhanced in vitro recombinational cloning of using ribosomal proteins
US6720140B1 (en) * 1995-06-07 2004-04-13 Invitrogen Corporation Recombinational cloning using engineered recombination sites
US6143557A (en) * 1995-06-07 2000-11-07 Life Technologies, Inc. Recombination cloning using engineered recombination sites
US5851808A (en) 1997-02-28 1998-12-22 Baylor College Of Medicine Rapid subcloning using site-specific recombination
US7119250B2 (en) * 1997-06-03 2006-10-10 The University Of Chicago Plant centromere compositions
EP1025217B1 (fr) * 1997-10-24 2006-10-04 Invitrogen Corporation Clonage recombinatoire au moyen d'acides nucleiques possedant des sites de recombinaison
US6632980B1 (en) 1997-10-24 2003-10-14 E. I. Du Pont De Nemours And Company Binary viral expression system in plants
US7351578B2 (en) * 1999-12-10 2008-04-01 Invitrogen Corp. Use of multiple recombination sites with unique specificity in recombinational cloning
US20030124555A1 (en) * 2001-05-21 2003-07-03 Invitrogen Corporation Compositions and methods for use in isolation of nucleic acid molecules
CN101125873A (zh) * 1997-10-24 2008-02-20 茵维特罗根公司 利用具重组位点的核酸进行重组克隆
WO1999025821A1 (fr) 1997-11-18 1999-05-27 Pioneer Hi-Bred International, Inc. Compositions et procedes de modification genetique de plantes
US7102055B1 (en) * 1997-11-18 2006-09-05 Pioneer Hi-Bred International, Inc. Compositions and methods for the targeted insertion of a nucleotide sequence of interest into the genome of a plant
AU745238C (en) * 1997-11-18 2003-02-27 Pioneer Hi-Bred International, Inc. Mobilization of viral genomes from T-DNA using site-specific recombination systems
AU3928899A (en) * 1998-04-28 1999-11-16 Novartis Ag Site-directed transformation of plants
EP1173460B1 (fr) * 1999-03-02 2009-09-16 Life Technologies Corporation Compositions et methodes de clonage recombinatoire d'acides nucleiques
US6746870B1 (en) 1999-07-23 2004-06-08 The Regents Of The University Of California DNA recombination in eukaryotic cells by the bacteriophage PHIC31 recombination system
NZ539430A (en) * 1999-12-10 2006-09-29 Invitrogen Corp Use of multiple recombination sites with unique specificity in recombinational cloning
ATE447036T1 (de) 1999-12-16 2009-11-15 Cropdesign Nv Optimisierter t-dna transfer und entsprechende vektoren
US20020023278A1 (en) * 2000-05-08 2002-02-21 Lyznik Leszek Alexander Genetic transformation in plants using site-specific recombination and wide hybridization
US7244560B2 (en) 2000-05-21 2007-07-17 Invitrogen Corporation Methods and compositions for synthesis of nucleic acid molecules using multiple recognition sites
BR0112633A (pt) * 2000-07-21 2003-09-16 Us Agriculture Método para substituir, translocar e empilhar dna em genomas eucarióticos
WO2002010415A2 (fr) * 2000-07-28 2002-02-07 University Of Connecticut Methodes d'excision controlees et automatiques d'adn heterologue issu de plantes transgeniques et cassettes de genes d'excision d'adn
US7198924B2 (en) 2000-12-11 2007-04-03 Invitrogen Corporation Methods and compositions for synthesis of nucleic acid molecules using multiple recognition sites
US7560622B2 (en) * 2000-10-06 2009-07-14 Pioneer Hi-Bred International, Inc. Methods and compositions relating to the generation of partially transgenic organisms
WO2005012515A2 (fr) 2003-04-29 2005-02-10 Pioneer Hi-Bred International, Inc. Genes de la glyphosate-n-acetyltransferase (gat)
DE10114209A1 (de) 2001-03-23 2002-12-05 Icon Genetics Ag Ortsgerichtete Transformation durch Verwendung von Amplifikationsvektoren
JP2004531259A (ja) * 2001-04-19 2004-10-14 インヴィトロジェン コーポレーション 核酸分子の組換えクローニングのための組成物および方法
HUP0402659A3 (en) * 2001-05-30 2008-07-28 Glaxo Group Ltd Chromosome-based platforms
WO2002096923A1 (fr) * 2001-05-30 2002-12-05 Chromos Molecular Systems, Inc. Chromosomes artificiels de plantes, utilisation de ces derniers et procedes de preparation de chromosomes artificiels de plantes
WO2003010333A2 (fr) * 2001-07-24 2003-02-06 Affinium Pharmaceuticals Inc. Methodes de disruption genique et leurs utilisations
JP2005521400A (ja) 2002-03-29 2005-07-21 シンジェンタ パーティシペーションズ アクチェンゲゼルシャフト 植物におけるラムダインテグラーゼ媒介型組換え
US7164056B2 (en) * 2002-05-03 2007-01-16 Pioneer Hi-Bred International, Inc. Gene targeting using replicating DNA molecules
US8304233B2 (en) 2002-06-04 2012-11-06 Poetic Genetics, Llc Methods of unidirectional, site-specific integration into a genome, compositions and kits for practicing the same
US7476777B2 (en) * 2002-09-17 2009-01-13 Ceres, Inc. Biological containment system
EP1405908A1 (fr) * 2002-10-04 2004-04-07 ProBioGen AG Création de lignes cellulaires d'expression hétérologue
US20040137624A1 (en) * 2002-12-27 2004-07-15 Lowe Brenda A. Methods of site-directed transformation
WO2005028615A2 (fr) * 2003-06-26 2005-03-31 Invitrogen Corporation Procedes et compositions permettant de detecter une activite de promoteur et d'exprimer des proteines de fusion
WO2005054438A2 (fr) 2003-12-01 2005-06-16 Invitrogen Corporation Molecule d'acide nucleique contenant des sites de recombinaison et leurs procedes d'utilisation
US7935862B2 (en) * 2003-12-02 2011-05-03 Syngenta Participations Ag Targeted integration and stacking of DNA through homologous recombination
WO2005059148A1 (fr) * 2003-12-17 2005-06-30 Pioneer Hi-Bred International, Inc. Systemes de piegeage de genes a mediation par recombinase
CN101124323A (zh) 2004-06-30 2008-02-13 先锋高级育种国际公司 保护植物免受致病真菌侵害的方法
BR122015026849C8 (pt) 2004-07-02 2017-06-20 Du Pont cassete de expressão, microorganismo transformado, método para indução de resistência a patógeno de planta em uma planta, composição anti-patogênica e método para proteção de uma planta contra um patógeno de planta
AU2005282746A1 (en) * 2004-09-02 2006-03-16 Wyeth Systems and methods for protein production
ES2390132T3 (es) * 2005-07-18 2012-11-06 Pioneer Hi-Bred International Inc. Sitios de recombinación FRT modificados y métodos de uso
CN101356279A (zh) 2005-11-10 2009-01-28 先锋高级育种国际公司 Dof(具有一指的dna结合)序列和使用方法
US20070143881A1 (en) * 2005-12-16 2007-06-21 Pioneer Hi-Bred International, Inc. Methods and Compositions for Improving the Efficiency of Site-Specific Polynucleotide Exchange
US8058509B2 (en) * 2005-12-21 2011-11-15 Pioneer Hi-Bred International, Inc. Methods and compositions for in planta production of inverted repeats
MX2008010989A (es) 2006-03-01 2009-01-26 Pionner Hi Bred International Composiciones relacionadas con el locus 6 de rasgo cuantitativo (qtl6) en maiz y metodos de uso.
AR060523A1 (es) 2006-04-19 2008-06-25 Pioneer Hi Bred Int Moleculas de polinucleotidos aislados que corresponden a alelos mutantes y tipo salvaje del gen de maiz d9 y metodos para usarlas
WO2007134122A2 (fr) 2006-05-09 2007-11-22 The Curators Of The University Of Missouri Plateformes végétales de chromosomes artificiels au moyen d'un troncage du télomère
EP2333088B1 (fr) 2006-05-16 2013-08-28 Pioneer Hi-Bred International, Inc. Polypeptides antifongiques
US20070271629A1 (en) 2006-05-17 2007-11-22 Pioneer Hi-Bred International, Inc. Artificial plant minichromosomes
US7951995B2 (en) 2006-06-28 2011-05-31 Pioneer Hi-Bred International, Inc. Soybean event 3560.4.3.5 and compositions and methods for the identification and detection thereof
WO2008008160A2 (fr) * 2006-07-07 2008-01-17 Fred Hutchinson Cancer Research Center recombinaison spécifique de site flp à haute efficacité dans les cellules mammaliennes par gène flp optimisé
EP1942192A1 (fr) * 2007-01-08 2008-07-09 Heinrich-Pette-Institut für experimentelle Virologie und Immunologie Utilisation de la récombinase sur mesure dans le traitement des infection retrovirales
BRPI0807350A2 (pt) 2007-02-05 2014-05-06 Univ Singapore Receptor de quitocina putativa e métodos para uso do mesmo
US20100154076A1 (en) 2007-05-25 2010-06-17 Cropdesign N.V. Yield Enhancement In Plants By Modulation of Maize Alfins
EP2167666A2 (fr) 2007-06-29 2010-03-31 Pioneer Hi-Bred International Inc. Procédés de modification du génome d'une cellule de plante monocotylédone
EP2031067A1 (fr) * 2007-08-30 2009-03-04 Albert-Ludwigs-Universität Freiburg Fabrication d'actinomycètes génétiquement modifiés par recombinaison
US8367895B2 (en) 2008-01-17 2013-02-05 Pioneer Hi-Bred International, Inc. Compositions and methods for the suppression of target polynucleotides from the family aphididae
US8847013B2 (en) 2008-01-17 2014-09-30 Pioneer Hi Bred International Inc Compositions and methods for the suppression of target polynucleotides from lepidoptera
MX341747B (es) 2008-02-29 2016-08-31 Monsanto Technology Llc Evento de maiz mon87460 y composiciones y metodos para detectarlo.
EP2271207A2 (fr) * 2008-03-26 2011-01-12 ITI Scotland Limited Insertion efficace d adn dans des cellules souches embryonnaires
CN102216453B (zh) 2008-09-26 2014-02-05 巴斯夫农化产品有限公司 除草剂-抗性的ahas-突变体及使用方法
CA2743707A1 (fr) 2008-12-04 2010-06-10 Pioneer Hi-Bred International, Inc. Procedes et compositions pour un rendement ameliore par une expression ciblee de knotted1
US8293533B2 (en) 2008-12-19 2012-10-23 E.I. Du Pont De Nemours And Company Site-specific integration and stacking of transgenes in soybean via DNA recombinase mediated cassette exchange
AU2010206619A1 (en) 2009-01-22 2011-07-28 Syngenta Participations Ag Mutant hydroxyphenylpyruvate dioxygenase polypeptides and methods of use
GB2467167B (en) 2009-01-26 2013-09-11 Algentech Sas Gene targeting in plants
CN102317461A (zh) 2009-02-19 2012-01-11 先锋国际良种公司 通过杂交种子生产期间的操纵进行的混合庇护区部署
MX2011011678A (es) 2009-05-04 2011-12-06 Pioneer Hi Bred Int Mejora de la produccion en plantas mediante la modulacion del factor de transcripcion ap2.
MX2011013224A (es) 2009-06-09 2012-06-01 Pioneer Hi Bred Int Promotor de endospermo temprano y metodos de uso.
AU2010326672A1 (en) 2009-07-10 2011-08-04 Syngenta Participations Ag Novel hydroxyphenylpyruvate dioxygenase polypeptides and methods of use
MX2012002189A (es) 2009-08-21 2012-03-16 Beeologics Inc Prevencion y cura de las enfermedades de los insectos beneficiosos a traves de moleculas.
EP3401404A1 (fr) 2009-08-28 2018-11-14 E. I. du Pont de Nemours and Company Compositions et méthodes pour le contrôle des insectes nuisibles
BR112012009044A2 (pt) 2009-10-26 2015-09-01 Pioneer Hi Bred Int Molécula de ácido nucléico isolada, cassete de expressão, vetor, célula vegetal, planta, semente transgênica, método para expressão de um polinucleotídeo em uma planta ou célula vegetal e método para expressão de um polinucleotídeo, preferencialmente em tecidos somáticos de óvulo de uma planta
WO2011082310A2 (fr) 2009-12-30 2011-07-07 Pioneer Hi-Bred International, Inc. Procédés et compositions pour la modification ciblée de polynucléotides
AU2010339404B2 (en) 2009-12-30 2016-01-28 Pioneer Hi-Bred International, Inc. Methods and compositions for the introduction and regulated expression of genes in plants
CA2788198C (fr) 2010-01-26 2021-01-19 Pioneer Hi-Bred International, Inc. Tolerance aux herbicides inhibiteurs du hppd
MX2012012211A (es) 2010-04-23 2012-12-17 Du Pont Composiciones de interruptores genicos y metodos de uso de las mismas.
MX2012012672A (es) 2010-05-06 2012-12-17 Du Pont Gen y proteina acc sintasa 3 de maiz y sus usos.
EP2576798B1 (fr) 2010-05-27 2016-04-27 Heinrich-Pette-Institut Leibniz-Institut für experimentelle Virologie-Stiftung bürgerlichen Rechts - Recombinase mise au point sur mesure pour recombiner des sites asymétriques voulus dans une pluralité de souches de rétrovirus
BR112012032907A2 (pt) 2010-06-25 2017-06-13 Du Pont métodos para selecionar e identificar uma planta de mais e planta de mais
BR112013003135A2 (pt) 2010-08-13 2017-11-07 Pioneer Hi Bred Int polinucletídeo e polipeptídeo isolado ou recombinante, construto de ácido nucleico, célula, planta, explante vegetal, semente transgênica, método para produção de célula vegetal para controle de plantas daninhas e para detecção de um polipeptídeo hppd e um polinucleotídeo.
BR112013003223A2 (pt) 2010-08-23 2016-06-07 Pioneer Hi Bred Int "polinucleotídeo isolado, cassete de expressão, célula hospedeira, micro-organismo, planta ou parte de planta, método de obtenção de uma planta transformada, composição antipatogênica, método para proteger uma planta contra um patógeno ou uso de um polinucleotídeo isolado"
EP2643464B1 (fr) 2010-11-24 2018-12-26 Pioneer Hi-Bred International, Inc. Événement dp-073496-4 de brassica gat et compositions et procédés pour l'identifier et/ou le détecter
CA2818918A1 (fr) 2010-11-24 2012-05-31 Pioneer Hi-Bred International, Inc. Evenement dp-061061-7 de brassica gat et compositions et procedes pour l'identifier et/ou le detecter
AR083029A1 (es) 2010-12-09 2013-01-23 Syngenta Participations Ag Metodos y composiciones que utilizan arn interferente pequeño (arnip) para el control de nematodos en plantas
TWI667347B (zh) 2010-12-15 2019-08-01 瑞士商先正達合夥公司 大豆品種ｓｙｈｔ０ｈ２及偵測其之組合物及方法
MX2013007532A (es) 2010-12-28 2013-09-16 Pioneer Hi Bred Int Nuevo gen de bacillus thuringiensis con actividad contra el orden lepidoptera.
CN103459601A (zh) 2011-02-11 2013-12-18 先锋国际良种公司 具有对抗玉米根虫的活性的合成杀昆虫蛋白
EP2675900B1 (fr) 2011-02-15 2017-09-06 Pioneer Hi-Bred International, Inc. Promoteur destiné de préférence aux racines et méthodes d'utilisation
US8878007B2 (en) 2011-03-10 2014-11-04 Pioneer Hi Bred International Inc Bacillus thuringiensis gene with lepidopteran activity
CA2831144A1 (fr) 2011-03-23 2012-09-27 Pioneer Hi-Bred International, Inc. Procedes de production d'un locus complexe de caracteristique transgenique
EP2794887A2 (fr) 2011-03-30 2014-10-29 Universidad Nacional Autonoma De Mexico GÈNES CRY MUTANTS DE BACILLUS THURINGIENSIS ET LEUR PROCÉDÉS D'UTILISATION& xA;
WO2012142311A1 (fr) 2011-04-15 2012-10-18 Pioneer Hi-Bred International, Inc. Plantes hybrides autoreproductrices
AU2012249390B2 (en) 2011-04-27 2017-03-30 Amyris, Inc. Methods for genomic modification
US9062317B2 (en) 2011-05-09 2015-06-23 E I Du Pont De Nemours And Company Methods and compositions for silencing gene families using artificial microRNAs
US9150625B2 (en) 2011-05-23 2015-10-06 E I Du Pont De Nemours And Company Chloroplast transit peptides and methods of their use
CA2844470A1 (fr) 2011-06-21 2013-05-10 Pioneer Hi-Bred International, Inc. Procedes et compositions pour obtenir des plantes steriles males
EP2739739A1 (fr) 2011-08-03 2014-06-11 E. I. Du Pont de Nemours and Company Procédés et compositions permettant une intégration ciblée dans une plante
BR112014004812A2 (pt) 2011-08-31 2018-10-23 Du Pont métodos para regenerar uma planta e para produzir uma planta transformada
CA2850390A1 (fr) 2011-10-28 2013-05-02 E. I. Du Pont De Nemours And Company Procedes et compositions pour le silencage de genes utilisant des microarn artificiels
WO2013063344A1 (fr) 2011-10-28 2013-05-02 Pioneer Hi-Bred International, Inc. Variants génétiquement modifiés de pep carboxylase pour une productivité végétale améliorée
WO2013103365A1 (fr) 2012-01-06 2013-07-11 Pioneer Hi-Bred International, Inc. Promoteurs de pollen préférés et procédés d'utilisation
WO2013103366A1 (fr) 2012-01-06 2013-07-11 Pioneer Hi-Bred International, Inc. Procédé pour cribler des plantes pour des éléments génétiques induisant la parthénogenèse dans des plantes
EP2800816A1 (fr) 2012-01-06 2014-11-12 Pioneer Hi-Bred International Inc. Promoteur spécifique d'ovule et méthodes d'utilisation
BR112014017196A2 (pt) 2012-01-11 2017-06-13 The Australian National University métodos para a modulação ou inibição da modulação da arquitetura da raiz de uma planta, planta, planta transgênica, peptídeo regulador de arquitetura de raiz isolado (rar), polinucleotídeo isolado, vetor recombinante, célula hospedeira transgênica e promotor de planta isolado
AR089793A1 (es) 2012-01-27 2014-09-17 Du Pont Metodos y composiciones para generar locus de rasgos transgenicos complejos
CN104145020B (zh) 2012-02-16 2020-06-02 先正达参股股份有限公司 工程化的杀有害生物蛋白质
WO2013134499A1 (fr) 2012-03-07 2013-09-12 The Johns Hopkins University Biocapteur d'anastasis
EP2839013B1 (fr) 2012-04-18 2020-08-26 The Board of Trustees of the Leland Stanford Junior University Ciblage génique non disruptif
US20150184173A1 (en) 2012-05-04 2015-07-02 E I Du Pont De Nemours And Company Compositions and methods comprising sequences having meganuclease activity
BR112014031260A2 (pt) 2012-06-15 2019-08-20 Du Pont métodos e composições que envolvem variantes de als com preferência de substrato nativo
US9347105B2 (en) 2012-06-15 2016-05-24 Pioneer Hi Bred International Inc Genetic loci associated with resistance of soybean to cyst nematode and methods of use
US9657293B2 (en) 2012-06-22 2017-05-23 Sygenta Participations Ag Biological control of coleopteran pests
CN104812901B (zh) 2012-09-13 2018-08-10 美国印第安纳大学研究和技术公司 赋予植物抗病性的组合物和系统及其使用方法
WO2014059155A1 (fr) 2012-10-11 2014-04-17 Pioneer Hi-Bred International, Inc. Promoteurs de cellules de garde et leur utilisation
CN104884625A (zh) 2012-10-15 2015-09-02 先锋国际良种公司 增强cry内毒素的活性的方法和组合物
US20140173775A1 (en) 2012-12-13 2014-06-19 Pioneer Hi-Bred International, Inc. Methods and compositions for producing and selecting transgenic plants
BR112015015055A2 (pt) 2012-12-21 2017-10-03 Pioneer Hi Bred Int Método para desintoxicar um herbicida análogo de auxina, método para controlar pelo menos uma erva em uma área de cultivo, método para testar uma resposta de planta a um ou mais compostos
US20150361447A1 (en) 2013-01-25 2015-12-17 Pioneer Hi-Breed International, Inc. Maize event dp-032218-9 and methods for detection thereof
CA2905399A1 (fr) 2013-03-11 2014-10-09 Pioneer Hi-Bred International, Inc. Procedes et compositions faisant appel a un domaine de stabilisation dependant de la sulfonyluree
WO2014164775A1 (fr) 2013-03-11 2014-10-09 Pioneer Hi-Bred International, Inc. Procédés et compositions permettant d'améliorer la propagation de signaux chimiques dans des plantes
RU2694686C2 (ru) 2013-03-12 2019-07-16 Е.И.Дюпон Де Немур Энд Компани Способы идентификации вариантных сайтов распознавания для редкощепящих сконструированных средств для индукции двунитевого разрыва, и композиции с ними, и их применения
US9243258B2 (en) 2013-03-12 2016-01-26 Pioneer Hi Bred International Inc Root-preferred promoter and methods of use
US9803214B2 (en) 2013-03-12 2017-10-31 Pioneer Hi-Bred International, Inc. Breeding pair of wheat plants comprising an MS45 promoter inverted repeat that confers male sterility and a construct that restores fertility
US9273322B2 (en) 2013-03-12 2016-03-01 Pioneer Hi Bred International Inc Root-preferred promoter and methods of use
BR112015023268A2 (pt) 2013-03-13 2018-05-02 Du Pont método para reduzir a expressão, construto de dna, método de obtenção de célula vegetal, método de obtenção de planta transgênica ou uma parte de planta
EA028528B1 (ru) 2013-03-13 2017-11-30 Пайонир Хай-Бред Интернэшнл, Инк. Внесение глифосата для борьбы с сорняками у brassica
AU2014236162A1 (en) 2013-03-14 2015-09-17 Arzeda Corp. Compositions having dicamba decarboxylase activity and methods of use
WO2014153254A2 (fr) 2013-03-14 2014-09-25 Pioneer Hi-Bred International Inc. Compositions et procédés pour contrôler des insectes ravageurs
BR112015023272A2 (pt) 2013-03-14 2017-07-18 Pioneer Hi Bred Int célula vegetal, planta, explante vegetal, semente transgênica, método para produzir uma célula vegetal tendo um polinucleotídeo heterólogo que codifica um polipeptídeo tendo atividade de dicamba descarboxilase, método para controlar plantas daninhas em um campo contendo uma cultura e método para controlar plantas daninhas em um campo contendo uma cultura
CA2901316A1 (fr) 2013-03-15 2014-09-25 Pioneer Hi-Bred International, Inc. Polypeptides phi-4 et leurs procedes d'utilisation
US11013917B2 (en) 2013-06-21 2021-05-25 Newsouth Innovations Pty Limited Method and apparatus for close-field electroporation
US10570409B2 (en) 2013-07-09 2020-02-25 Board Of Trustees Of Michigan State University Transgenic plants produced with a K-domain, and methods and expression cassettes related thereto
US11459579B2 (en) 2013-07-09 2022-10-04 Board Of Trustees Of Michigan State University Transgenic plants produced with a K-domain, and methods and expression cassettes related thereto
CA2918911A1 (fr) 2013-07-31 2015-02-05 E.I. Du Pont De Nemours And Company Modification de la composition de graine de soja pour ameliorer la nourriture pour animaux, les aliments et d'autres applications industrielles de produits a base de soja
EP3030072B1 (fr) 2013-08-08 2020-03-04 Pioneer Hi-Bred International, Inc. Polypeptides insecticides ayant un spectre d'activité large et leurs utilisations
BR112016003225B1 (pt) 2013-08-16 2022-10-25 Pioneer Hi-Bred International, Inc. Polipeptídeo pip-47, polipeptídeo pip-47 quimérico, composição, proteína de fusão, método para controlar uma população de inseto-praga, método para inibir o crescimento ou matar um inseto-praga, construto de dna, polinucleotídeo isolado, cassete de expressão, método de obtenção de uma planta transgênica e método para controlar infestação de inseto
US20160201073A1 (en) 2013-09-11 2016-07-14 Pioneer Hi-Bred International, Inc. Plant regulatory elements and methods of use thereof
BR122020001770B1 (pt) 2013-09-13 2022-11-29 Pioneer Hi-Bred International, Inc Construto de dna, método de obtenção de planta transgênica, proteína de fusão, método para controlar uma população de praga de inseto, método para inibir o crescimento ou matar uma praga de inseto
EP3057434A4 (fr) 2013-10-16 2017-05-03 The Australian National University Méthode de modulation de la croissance des plantes
US10329578B2 (en) 2013-10-18 2019-06-25 Pioneer Hi-Bred International, Inc. Glyphosate-N-acetyltransferase (GLYAT) sequences and methods of use
US20160249542A1 (en) 2013-10-29 2016-09-01 Pioneer Hi-Bred International Inc. Self-reproducing hybrid plants
EP3083958B1 (fr) 2013-12-19 2019-04-17 Amyris, Inc. Procédés d'intégration génomique
BR112016018103B1 (pt) 2014-02-07 2024-01-16 E.I. Du Pont De Nemours And Company Polipeptídeo e seu uso, polinucleotídeo, composição, proteína de fusão, método para controlar uma população, método para inibir o crescimento, método para controlar a infestação, método para obtenção de uma planta ou célula vegetal, construto
CN106536545B (zh) 2014-02-07 2026-03-03 先锋国际良种公司 杀昆虫蛋白及其使用方法
BR112016019940A2 (pt) 2014-03-21 2017-10-24 Univ Leland Stanford Junior edição de genoma sem nucleases
WO2015164457A1 (fr) 2014-04-22 2015-10-29 E. I. Du Pont De Nemours And Company Gènes d'anhydrase carbonique plastidiale pour l'augmentation d'huile dans des graines présentant une expression augmentée de dgat
WO2016000237A1 (fr) 2014-07-03 2016-01-07 Pioneer Overseas Corporation Plantes ayant une tolérance accrue à des insectes nuisibles et constructions s'y rapportant et procédés mettant en jeu des gènes de tolérance aux insectes
CA2955828A1 (fr) 2014-08-08 2016-02-11 Pioneer Hi-Bred International, Inc. Promoteurs d'ubiquitine et introns, et procedes d'utilisation
EP2993229A1 (fr) 2014-09-02 2016-03-09 Heinrich-Pette-Institut Leibniz-Institut für experimentelle Virologie-Stiftung bürgerlichen Rechts - Recombinase bien toléré et très spécifique mise au point sur mesure pour recombiner des sites asymétriques voulus dans une pluralité de souches de rétrovirus
MX2017002930A (es) 2014-09-12 2017-06-06 Du Pont Generacion de sitios de integracion especifica de sitio para loci de rasgos complejos en maiz y soja, y metodos de uso.
WO2016044092A1 (fr) 2014-09-17 2016-03-24 Pioneer Hi Bred International Inc Compositions et procédés de lutte contre des insectes ravageurs
CN107072165B (zh) 2014-09-26 2021-05-28 先锋国际良种公司 小麦Ms1多核苷酸、多肽以及使用方法
UA124757C2 (uk) 2014-10-16 2021-11-17 Піонір Хай-Бред Інтернешнл, Інк. Інсектицидний поліпептид проти лускокрилого або твердокрилого шкідника та його застосування
CN113372421B (zh) 2014-10-16 2024-08-06 先锋国际良种公司 杀昆虫蛋白及其使用方法
CN114736275A (zh) 2014-10-16 2022-07-12 先锋国际良种公司 具有改进的活性谱的杀昆虫多肽及其用途
MX392360B (es) 2014-12-12 2025-03-24 Syngenta Participations Ag Composiciones y metodos para controlar plagas en plantas.
WO2016100333A1 (fr) 2014-12-15 2016-06-23 Syngenta Participations Ag Supports de micro-arn pesticides et leur utilisation
WO2016100309A1 (fr) 2014-12-16 2016-06-23 Pioneer Hi-Bred International, Inc. Rétablissement de la fertilité mâle dans le blé
US20170359965A1 (en) 2014-12-19 2017-12-21 E I Du Pont De Nemours And Company Polylactic acid compositions with accelerated degradation rate and increased heat stability
PT3237617T (pt) 2014-12-23 2019-05-30 Syngenta Participations Ag Controlo biológico de pragas de coleópteros
CN114075267B (zh) 2015-01-15 2025-03-18 先锋国际良种公司 杀昆虫蛋白及其使用方法
CA2977026A1 (fr) 2015-03-11 2016-09-15 E.I. Du Pont De Nemours And Company Combinaisons de pip-72 insecticides et procedes d'utilisation
EP4118955A1 (fr) 2015-03-19 2023-01-18 Pioneer Hi-Bred International, Inc. Compositions d'introgression de trait accélérée
EP3091076A1 (fr) 2015-05-07 2016-11-09 Limagrain Europe Polynucléotides responsables de l'induction d'haploïdes dans des plants de maïs et procédés associés
CA2985198A1 (fr) 2015-05-19 2016-11-24 Pioneer Hi-Bred International, Inc. Proteines insecticides et leurs procedes d'utilisation
EP3310803A1 (fr) 2015-06-16 2018-04-25 Pioneer Hi-Bred International, Inc. Compositions et procédés de lutte contre des insectes nuisibles
BR112018002535A2 (pt) 2015-08-06 2018-09-25 Du Pont polipeptídeo inseticida recombinante, polinucleotídeo recombinante, construto de dna, planta transgênica ou célula de planta, composição, proteína de fusão, método para controlar uma praga, método para inibir crescimento ou para exterminar uma população de pragas ou uma praga e uso do polipeptídeo
DK3341483T3 (da) 2015-08-28 2020-03-16 Pioneer Hi Bred Int Ochrobactrum-medieret transformation af planter
WO2021257206A1 (fr) 2020-06-17 2021-12-23 Pioneer Hi-Bred International, Inc. Production de plants de maïs présentant une résistance accrue à l'helminthosporiose du nord du maïs
WO2017066597A1 (fr) 2015-10-16 2017-04-20 Pioneer Hi-Bred International, Inc. Génération des plantes de maïs présentant une meilleure résistance à l'helminthosporiose du nord du maïs
WO2017079026A1 (fr) 2015-11-06 2017-05-11 E. I. Du Pont De Nemours And Company Génération de locus à traits complexes dans le soja et procédés d'utilisation
CA3004056C (fr) 2015-12-22 2024-01-23 Pioneer Hi-Bred International, Inc. Promoteurs preferes par un tissu et procedes d'utilisation
AU2017234920B2 (en) 2016-03-18 2023-08-17 E. I. Du Pont De Nemours And Company Methods and compositions for producing clonal, non-reduced, non-recombined gametes
RU2018137045A (ru) 2016-04-14 2020-05-14 Пайонир Хай-Бред Интернэшнл, Инк. Инсектицидные полипептиды, обладающие улучшенным спектром активности, и пути их применения
EP3445861B1 (fr) 2016-04-19 2021-12-08 Pioneer Hi-Bred International, Inc. Combinaisons insecticides de polypeptides ayant un spectre d'activité amélioré et leurs utilisations
CA3018384A1 (fr) 2016-05-04 2017-11-09 Pioneer Hi-Bred International, Inc. Proteines insecticides et procedes pour les utiliser
US11293033B2 (en) 2016-05-18 2022-04-05 Amyris, Inc. Compositions and methods for genomic integration of nucleic acids into exogenous landing pads
CA3022858A1 (fr) 2016-06-16 2017-12-21 Pioneer Hi-Bred International, Inc. Compositions et procedes de lutte contre des insectes nuisibles
UA127388C2 (uk) 2016-06-24 2023-08-09 Піонір Хай-Бред Інтернешнл, Інк. Регуляторний елемент рослини і спосіб його застосування
EP3478052B1 (fr) 2016-07-01 2021-08-25 Pioneer Hi-Bred International, Inc. Protéines insecticides issues de plantes et procédés pour leur utilisation
WO2018013333A1 (fr) 2016-07-12 2018-01-18 Pioneer Hi-Bred International, Inc. Compositions et procédés de lutte contre des insectes nuisibles
AR109205A1 (es) 2016-08-05 2018-11-07 Syngenta Participations Ag Control de plagas de coleópteros utilizando moléculas de arn
AR109206A1 (es) 2016-08-05 2018-11-07 Syngenta Participations Ag Control de plagas de coleópteros utilizando moléculas de arn
WO2018076335A1 (fr) 2016-10-31 2018-05-03 Institute Of Genetics And Developmental Biology, Chinese Academy Of Sciences Compositions et procédés pour améliorer la tolérance au stress abiotique
EP3535285B1 (fr) 2016-11-01 2022-04-06 Pioneer Hi-Bred International, Inc. Protéines insecticides et leurs procédés d'utilisation
US11650201B2 (en) 2016-11-16 2023-05-16 The Johns Hopkins University Anastasis biosensor
BR112019012339A2 (pt) 2016-12-14 2019-11-26 Pioneer Hi Bred Int polipeptídeo inseticida recombinante, composição, construto de dna, célula hospedeira, planta transgênica, método para inibir o crescimento ou extermínio de uma praga de inseto ou população de praga, polipeptídeo ipd093 quimérico e proteína de fusão
US11213028B2 (en) 2016-12-22 2022-01-04 Pioneer Hi-Bred International, Inc. Insecticidal proteins and methods for their use
WO2018148001A1 (fr) 2017-02-08 2018-08-16 Pioneer Hi-Bred International Inc Associations insecticides de protéines insecticides d'origine végétale et leurs procédés d'utilisation
RU2019140646A (ru) 2017-05-11 2021-06-11 Пайонир Хай-Бред Интернэшнл, Инк. Инсектицидные белки и способы их применения
CN110914438A (zh) 2017-05-26 2020-03-24 先锋国际良种公司 具有改善的活性谱的杀昆虫多肽及其用途
FI3638776T3 (fi) 2017-06-14 2025-12-09 Univ Dresden Tech Menetelmiä ja välineitä genomien geneettiseen muokkaamiseen hyödyntäen suunnittelu-DNA:ta rekombinoivia entsyymejä
US11649465B2 (en) 2017-09-11 2023-05-16 R.J. Reynolds Tobacco Company Methods and compositions for increasing expression of genes of interest in a plant by co-expression with p21
CN111373046A (zh) 2017-09-25 2020-07-03 先锋国际良种公司 组织偏好性启动子和使用方法
MX2020003414A (es) 2017-10-02 2020-07-20 Syngenta Participations Ag Proteinas pesticidas modificadas y metodos para controlar las plagas de plantas.
US20200165626A1 (en) 2017-10-13 2020-05-28 Pioneer Hi-Bred International, Inc. Virus-induced gene silencing technology for insect control in maize
AR113761A1 (es) 2017-10-18 2020-06-10 Syngenta Participations Ag Control de plagas de hemípteros utilizando moléculas de arn
US11649509B2 (en) 2017-11-16 2023-05-16 The Johns Hopkins University Anastasis biosensor caspase tracker
EP4122947A1 (fr) 2017-12-19 2023-01-25 Pioneer Hi-Bred International, Inc. Polypeptides insecticides et leurs utilisations
WO2019157522A1 (fr) 2018-02-12 2019-08-15 Curators Of The University Of Missouri Petit gène de l'auxine régulé à la hausse (saur) pour l'amélioration de l'architecture du système racinaire, de la tolérance à la saturation en eau, de la résistance à la sécheresse et du rendement chez les plantes
WO2019162163A1 (fr) 2018-02-26 2019-08-29 Devgen Nv Lutte contre les insectes nuisibles à l'aide de molécules d'arn
EP3759489A1 (fr) 2018-03-02 2021-01-06 Pioneer Hi-Bred International, Inc. Analyse phytosanitaire
US11332752B2 (en) 2018-03-12 2022-05-17 Pioneer Hi-Bred International, Inc. Use of morphogenic factors for the improvement of gene editing
AU2019234562B2 (en) 2018-03-14 2024-08-01 Pioneer Hi-Bred International, Inc. Insecticidal proteins from plants and methods for their use
CA3092078A1 (fr) 2018-03-14 2019-09-19 Pioneer Hi-Bred International, Inc. Proteines insecticides issues de plantes et procedes pour leur utilisation
US20210123069A1 (en) 2018-04-27 2021-04-29 Devgen Nv Control of insect pests using rna molecules
WO2019226508A1 (fr) 2018-05-22 2019-11-28 Pioneer Hi-Bred International, Inc. Éléments régulateurs de plante et leurs procédés d'utilisation
CA3097915A1 (fr) 2018-06-28 2020-01-02 Pioneer Hi-Bred International, Inc. Procedes de selection de plantes transformees
WO2020046701A1 (fr) 2018-08-29 2020-03-05 Pioneer Hi-Bred International, Inc. Protéines insecticides et leurs procédés d'utilisation
US20210395758A1 (en) 2018-10-31 2021-12-23 Pioneer Hi-Bred International, Inc. Compositions and methods for ochrobactrum-mediated plant transformation
PH12021551717A1 (en) 2019-02-20 2022-03-28 Syngenta Crop Protection Ag Engineered pesticidal proteins and methods of controlling plant pests
JP2022524615A (ja) 2019-03-11 2022-05-09 パイオニア ハイ－ブレッド インターナショナル， インコーポレイテッド クローン植物の作製方法
CA3129553A1 (fr) 2019-03-21 2020-09-24 Lien DE SCHRIJVER Lutte contre les insectes nuisibles a l'aide de molecules d'arn
EP3947425A1 (fr) 2019-03-27 2022-02-09 Pioneer Hi-Bred International, Inc. Transformation d'explant de plante
EP3947696A1 (fr) 2019-03-28 2022-02-09 Pioneer Hi-Bred International, Inc. Souches d'agrobacterium modifiées et leur utilisation pour la transformation de plantes
BR112021020770A2 (pt) 2019-04-18 2022-01-04 Pioneer Hi Bred Int Fatores de embriogênese para reprogramação celular de uma célula vegetal
EP3831939A1 (fr) * 2019-12-06 2021-06-09 Technische Universität Dresden Fusion de recombinases spécifiques à un site pour une édition efficace et spécifique du génome
TWI906251B (zh) 2020-02-04 2025-12-01 美商科迪華農業科技有限責任公司 具有殺有害生物效用之組成物及與其相關之方法
CA3171935A1 (fr) 2020-03-15 2021-09-23 Mahmoud ELJENDY Production de proteine recombinante chez des insectes
CA3175936A1 (fr) 2020-06-12 2021-12-16 Pioneer Hi-Bred International, Inc. Alteration de la composition de graines dans des plantes
BR112022027035A2 (pt) 2020-07-14 2023-04-11 Pioneer Hi Bred Int Proteínas inseticidas e métodos para uso das mesmas
CN116096903A (zh) 2020-08-10 2023-05-09 先锋国际良种公司 植物调节元件及其使用方法
CA3189779A1 (fr) 2020-08-10 2022-02-17 E. I. Du Pont De Nemours And Company Compositions et procedes pour ameliorer la resistance a l'helminthosporiose du mais
WO2022072335A2 (fr) 2020-09-30 2022-04-07 Pioneer Hi-Bred International, Inc. Transformation rapide d'explants de feuilles de monocotylédone
CA3197681A1 (fr) 2020-10-21 2022-04-28 Pioneer Hi-Bred International, Inc. Facteurs de parthenogenese et leurs procedes d'utilisation
CN116635529A (zh) 2020-10-21 2023-08-22 先锋国际良种公司 双单倍体诱导物
CA3198940A1 (fr) 2020-11-24 2022-06-02 Rebekah Deter Kelly Genes pesticides et procedes d'utilisation
US20240141311A1 (en) 2021-04-22 2024-05-02 North Carolina State University Compositions and methods for generating male sterile plants
BR112023023044A2 (pt) 2021-05-06 2024-01-23 Agbiome Inc Genes pesticidas e métodos de uso
US20220386574A1 (en) * 2021-06-03 2022-12-08 Mayo Foundation For Medical Education And Research P21-cre animal models
WO2023076898A1 (fr) 2021-10-25 2023-05-04 The Broad Institute, Inc. Procédés et compositions pour l'édition d'un génome à l'aide d'une édition primaire et d'une recombinase
EP4444890A1 (fr) 2021-12-07 2024-10-16 Agbiome, Inc. Gènes pesticides et procédés d'utilisation
TW202345696A (zh) 2022-05-18 2023-12-01 美商科迪華農業科技有限責任公司 具有殺有害生物效用之組成物及與其相關的方法
EP4562168A1 (fr) 2022-07-28 2025-06-04 Institut Pasteur Hsc70-4 utilisé dans le silençage génique induit par l'hôte et induit par pulvérisation
WO2024044596A1 (fr) 2022-08-23 2024-02-29 AgBiome, Inc. Gènes pesticides et procédés d'utilisation
EP4360451A1 (fr) 2022-10-25 2024-05-01 Limagrain Europe Mutants pour induction d'haploïdes
AR131334A1 (es) 2022-12-13 2025-03-12 Ag Biome Inc Genes plaguicidas y métodos de uso
EP4455293A1 (fr) 2023-04-25 2024-10-30 Limagrain Europe Procédés pour fournir une plante présentant une résistance accrue à un virus de la famille des lutéoviridae
WO2024223802A2 (fr) 2023-04-26 2024-10-31 Syngenta Crop Protection Ag Lutte contre des nuisibles des cultures à l'aide de molécules d'arn

Family Cites Families (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1992015694A1 (fr) 1991-03-08 1992-09-17 The Salk Institute For Biological Studies Modification de genes induite par recombinase dans des cellules de mammifere, compositions et cellules utiles a cet effet
WO1993001283A1 (fr) 1991-07-08 1993-01-21 The United States Of America As Represented By The Secretary Of Agriculture Plantes transgeniques sans genes de selection
ES2156149T3 (es) 1992-12-04 2001-06-16 Medical Res Council Proteinas de union multivalente y multiespecificas, su fabricacion y su uso.
US5527695A (en) * 1993-01-29 1996-06-18 Purdue Research Foundation Controlled modification of eukaryotic genomes
EP0632054A1 (fr) 1993-06-28 1995-01-04 European Molecular Biology Laboratory Régulation de la recombinaison à site spécifique par des protéines de fusion de recombinase à site spécifique/récepteur nucléaire
WO1995015388A1 (fr) 1993-12-03 1995-06-08 Medical Research Council Proteines et peptides de liaison recombines
EG23907A (en) 1994-08-01 2007-12-30 Delta & Pine Land Co Control of plant gene expression
EP2322614A1 (fr) * 1995-06-07 2011-05-18 Life Technologies Corporation Clonage de recombinaison au moyen de sites de recombinaison obtenus par génie génétique
US5801030A (en) 1995-09-01 1998-09-01 Genvec, Inc. Methods and vectors for site-specific recombination
AUPN523995A0 (en) 1995-09-05 1995-09-28 Crc For Biopharmaceutical Research Pty Ltd Method for producing phage display vectors
US5677175A (en) 1995-10-13 1997-10-14 Purdue Research Foundation Plant pathogen induced proteins
AUPN903196A0 (en) 1996-03-29 1996-04-26 Australian National University, The Single-step excision means
US5928914A (en) 1996-06-14 1999-07-27 Albert Einstein College Of Medicine Of Yeshiva University, A Division Of Yeshiva University Methods and compositions for transforming cells
US6774279B2 (en) * 1997-05-30 2004-08-10 Carnegie Institution Of Washington Use of FLP recombinase in mice
US6114600A (en) 1997-10-31 2000-09-05 The United States Of America As Represented By The Secretary Of Agriculture Resolution of complex integration patterns to obtain single copy transgenes
WO1999025821A1 (fr) 1997-11-18 1999-05-27 Pioneer Hi-Bred International, Inc. Compositions et procedes de modification genetique de plantes
AU745238C (en) 1997-11-18 2003-02-27 Pioneer Hi-Bred International, Inc. Mobilization of viral genomes from T-DNA using site-specific recombination systems
AU3928899A (en) 1998-04-28 1999-11-16 Novartis Ag Site-directed transformation of plants

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PT1032680E (pt) 2006-05-31
AU745960C (en) 2003-09-18
AU1590499A (en) 1999-06-07
NZ504511A (en) 2002-12-20
AU745960B2 (en) 2002-04-11
US7223601B2 (en) 2007-05-29
WO1999025840A1 (fr) 1999-05-27
DE69833457T2 (de) 2006-09-14
CA2306188C (fr) 2008-08-05
EP1032680B1 (fr) 2006-02-08
US20030119166A1 (en) 2003-06-26
US6262341B1 (en) 2001-07-17
BR9814669A (pt) 2001-11-20
ES2256972T3 (es) 2006-07-16
CA2306188A1 (fr) 1999-05-27
EP1032680A1 (fr) 2000-09-06
DE69833457D1 (de) 2006-04-20
US6541231B1 (en) 2003-04-01
ATE317440T1 (de) 2006-02-15

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