DK1438437T3 - Fremgangsmåde til påvisning af cytosin-methyleringer i immobiliserede DNA-pröver - Google Patents

Fremgangsmåde til påvisning af cytosin-methyleringer i immobiliserede DNA-pröver

Info

Publication number: DK1438437T3
Authority: DK; Denmark
Prior art keywords: dna; immobilized; dna samples; immobilized dna; analyzing
Prior art date: 2001-10-26

Application number

DK02802274T

Other languages

English (en)

Inventor

Matthias Ballhause

Kurt Berlin

David Guetig

Original Assignee

Epigenomics Ag

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

2001-10-26

Filing date

2002-10-25

Publication date

2009-07-20

2002-10-25 Application filed by Epigenomics Ag filed Critical Epigenomics Ag

2009-07-20 Application granted granted Critical

2009-07-20 Publication of DK1438437T3 publication Critical patent/DK1438437T3/da

Links

230000030933 DNA methylation on cytosine Effects 0.000 title abstract 3
238000000034 method Methods 0.000 title 1
108020004414 DNA Proteins 0.000 abstract 6
239000003153 chemical reaction reagent Substances 0.000 abstract 2
OPTASPLRGRRNAP-UHFFFAOYSA-N cytosine Chemical compound NC=1C=CNC(=O)N=1 OPTASPLRGRRNAP-UHFFFAOYSA-N 0.000 abstract 2
LRSASMSXMSNRBT-UHFFFAOYSA-N 5-methylcytosine Chemical compound CC1=CNC(=O)N=C1N LRSASMSXMSNRBT-UHFFFAOYSA-N 0.000 abstract 1
108091093088 Amplicon Proteins 0.000 abstract 1
LSNNMFCWUKXFEE-UHFFFAOYSA-M Bisulfite Chemical compound OS([O-])=O LSNNMFCWUKXFEE-UHFFFAOYSA-M 0.000 abstract 1
239000003155 DNA primer Substances 0.000 abstract 1
238000006243 chemical reaction Methods 0.000 abstract 1
229940104302 cytosine Drugs 0.000 abstract 1
239000007790 solid phase Substances 0.000 abstract 1
238000005406 washing Methods 0.000 abstract 1

Classifications

- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism

Landscapes

Chemical & Material Sciences (AREA)
Organic Chemistry (AREA)
Life Sciences & Earth Sciences (AREA)
Zoology (AREA)
Wood Science & Technology (AREA)
Proteomics, Peptides & Aminoacids (AREA)
Health & Medical Sciences (AREA)
Engineering & Computer Science (AREA)
Microbiology (AREA)
Immunology (AREA)
Physics & Mathematics (AREA)
Molecular Biology (AREA)
Biotechnology (AREA)
Biophysics (AREA)
Analytical Chemistry (AREA)
Biochemistry (AREA)
Bioinformatics & Cheminformatics (AREA)
General Engineering & Computer Science (AREA)
General Health & Medical Sciences (AREA)
Genetics & Genomics (AREA)
Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

DK02802274T 2001-10-26 2002-10-25 Fremgangsmåde til påvisning af cytosin-methyleringer i immobiliserede DNA-pröver DK1438437T3 (da)

Applications Claiming Priority (2)

Application Number	Priority Date	Filing Date	Title
DE10154317A DE10154317B4 (de)	2001-10-26	2001-10-26	Verfahren zum Nachweis von Cytosin-Methylierungen in immobilisierten DNA Proben
PCT/DE2002/004054 WO2003038121A2 (de)	2001-10-26	2002-10-25	Verfahren zum nachweis von cytosin-methylierungen in immobilisierten dna proben

Publications (1)

Publication Number	Publication Date
DK1438437T3 true DK1438437T3 (da)	2009-07-20

Family

ID=7704694

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
DK02802274T DK1438437T3 (da)	2001-10-26	2002-10-25	Fremgangsmåde til påvisning af cytosin-methyleringer i immobiliserede DNA-pröver

Country Status (10)

Country	Link
US (3)	US7407749B2 (da)
EP (2)	EP2085487A1 (da)
JP (1)	JP2005506850A (da)
AT (1)	ATE429512T1 (da)
AU (1)	AU2002363259B2 (da)
CA (1)	CA2462932C (da)
DE (2)	DE10154317B4 (da)
DK (1)	DK1438437T3 (da)
ES (1)	ES2325121T3 (da)
WO (1)	WO2003038121A2 (da)

Families Citing this family (20)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
US7332275B2 (en)	1999-10-13	2008-02-19	Sequenom, Inc.	Methods for detecting methylated nucleotides
US20060134643A1 (en)	2000-06-19	2006-06-22	Kurt Berlin	Bisulfite conversion of DNA
US20110151438A9 (en)	2001-11-19	2011-06-23	Affymetrix, Inc.	Methods of Analysis of Methylation
US9394332B2 (en)	2002-08-29	2016-07-19	Epigenomics Ag	Method for bisulfite treatment
DE10338308B4 (de)	2003-08-15	2006-10-19	Epigenomics Ag	Verfahren zum Nachweis von Cytosin-Methylierungen in DNA
WO2005024068A2 (en)	2003-09-05	2005-03-17	Sequenom, Inc.	Allele-specific sequence variation analysis
ES2432025T3 (es) *	2003-10-09	2013-11-29	Epigenomics Ag	Conversión mejorada de ADN con bisulfito
US7608394B2 (en)	2004-03-26	2009-10-27	Sequenom, Inc.	Methods and compositions for phenotype identification based on nucleic acid methylation
US9249456B2 (en)	2004-03-26	2016-02-02	Agena Bioscience, Inc.	Base specific cleavage of methylation-specific amplification products in combination with mass analysis
EP2458015B1 (en) *	2004-09-30	2015-12-23	Epigenomics AG	Method for providing DNA fragments derived from an archived sample
WO2006084340A1 (en) *	2005-02-14	2006-08-17	Commonwealth Scientific And Industrial Research Organisation	Solid phase assay for methylation analysis
US20060223071A1 (en) *	2005-04-01	2006-10-05	Wisniewski Michele E	Methods, compositions, and kits for detecting nucleic acids in a single vessel
US20060223070A1 (en) *	2005-04-01	2006-10-05	Wisniewski Michele E	Methods, compositions, and kits for detecting nucleic acids in a single vessel
ES2647483T3 (es)	2005-04-01	2017-12-21	Epigenomics Ag	Conversión por bisulfito mejorada de ADN
US20060292585A1 (en) *	2005-06-24	2006-12-28	Affymetrix, Inc.	Analysis of methylation using nucleic acid arrays
ATE452993T1 (de)	2005-07-21	2010-01-15	Epigenomics Ag	Verfahren zur quantifizierung methylierter dna
US7901882B2 (en)	2006-03-31	2011-03-08	Affymetrix, Inc.	Analysis of methylation using nucleic acid arrays
DE102006046776B4 (de) *	2006-09-29	2019-07-18	Siemens Aktiengesellschaft	Anordnung und Verfahren zum Nachweis kleiner Stoffkonzentrationen
US20080213870A1 (en) *	2007-03-01	2008-09-04	Sean Wuxiong Cao	Methods for obtaining modified DNA from a biological specimen
CN116445593A (zh)	2016-08-10	2023-07-18	格里尔公司	测定一生物样品的一甲基化图谱的方法

Family Cites Families (18)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
SE501439C2 (sv)	1993-06-22	1995-02-13	Pharmacia Lkb Biotech	Sätt och anordning för analys av polynukleotidsekvenser
EP0671958B1 (en)	1993-08-10	2001-07-18	Slobodan Tepic	Apparatus for the treatment of tumors by selective protein depletion
AU1061395A (en)	1993-11-30	1995-06-19	Mcgill University	Inhibition of dna methyltransferase
JPH11514872A (ja)	1995-10-18	1999-12-21	ダーウィンモレキュラーコーポレイション	ハイブリダイゼーション用の固体支持体を調製する方法および非特異的バックグラウンドを低減する方法
US6017704A (en)	1996-06-03	2000-01-25	The Johns Hopkins University School Of Medicine	Method of detection of methylated nucleic acid using agents which modify unmethylated cytosine and distinguishing modified methylated and non-methylated nucleic acids
ATE347615T1 (de) *	1997-04-16	2006-12-15	Applera Corp	Nukleinsäuresammlung
DE19746874A1 (de) *	1997-10-23	1999-04-29	Qiagen Gmbh	Verfahren zur Isolierung und Reinigung von Nukleinsäuren an hydrophoben Oberflächen - insbesondere unter Verwendung hydrophober Membranen
DE19754482A1 (de)	1997-11-27	1999-07-01	Epigenomics Gmbh	Verfahren zur Herstellung komplexer DNA-Methylierungs-Fingerabdrücke
DE19905082C1 (de) *	1999-01-29	2000-05-18	Epigenomics Gmbh	Verfahren zur Identifikation von Cytosin-Methylierungsmustern in genomischen DNA-Proben
US6331393B1 (en)	1999-05-14	2001-12-18	University Of Southern California	Process for high-throughput DNA methylation analysis
US20020055184A1 (en) *	1999-09-08	2002-05-09	Stephen Naylor	Systems for detecting analytes
DE10029915B4 (de) *	2000-06-19	2005-06-16	Epigenomics Ag	Verfahren zum Nachweis von Cytosin-Methylierungen
US20040054162A1 (en) *	2001-10-30	2004-03-18	Hanna Michelle M.	Molecular detection systems utilizing reiterative oligonucleotide synthesis
US9394332B2 (en) *	2002-08-29	2016-07-19	Epigenomics Ag	Method for bisulfite treatment
US7534873B2 (en)	2003-08-29	2009-05-19	Applied Biosystems, Llc	Method and materials for quaternary amine catalyzed bisulfite conversion of cytosine to uracil
US7371526B2 (en)	2003-08-29	2008-05-13	Applera Corporation	Method and materials for bisulfite conversion of cytosine to uracil
US7368239B2 (en)	2003-08-29	2008-05-06	Applera Corporation	Method and materials for polyamine catalyzed bisulfite conversion of cytosine to uracil
ES2432025T3 (es)	2003-10-09	2013-11-29	Epigenomics Ag	Conversión mejorada de ADN con bisulfito

2001
- 2001-10-26 DE DE10154317A patent/DE10154317B4/de not_active Expired - Fee Related
2002
- 2002-10-25 ES ES02802274T patent/ES2325121T3/es not_active Expired - Lifetime
- 2002-10-25 EP EP09157992A patent/EP2085487A1/de not_active Withdrawn
- 2002-10-25 EP EP02802274A patent/EP1438437B1/de not_active Expired - Lifetime
- 2002-10-25 DK DK02802274T patent/DK1438437T3/da active
- 2002-10-25 AU AU2002363259A patent/AU2002363259B2/en not_active Ceased
- 2002-10-25 US US10/416,624 patent/US7407749B2/en not_active Expired - Fee Related
- 2002-10-25 DE DE50213489T patent/DE50213489D1/de not_active Expired - Lifetime
- 2002-10-25 AT AT02802274T patent/ATE429512T1/de active
- 2002-10-25 JP JP2003540386A patent/JP2005506850A/ja active Pending
- 2002-10-25 WO PCT/DE2002/004054 patent/WO2003038121A2/de not_active Ceased
- 2002-10-25 CA CA2462932A patent/CA2462932C/en not_active Expired - Fee Related
2007
- 2007-10-31 US US11/981,357 patent/US7534570B2/en not_active Expired - Fee Related
2009
- 2009-05-18 US US12/454,429 patent/US20090280494A1/en not_active Abandoned

Also Published As

Publication number	Publication date
DE10154317B4 (de)	2005-06-09
JP2005506850A (ja)	2005-03-10
ATE429512T1 (de)	2009-05-15
WO2003038121A3 (de)	2003-12-11
WO2003038121A2 (de)	2003-05-08
CA2462932C (en)	2011-04-26
CA2462932A1 (en)	2003-05-08
US20090280494A1 (en)	2009-11-12
EP1438437B1 (de)	2009-04-22
US20040115663A1 (en)	2004-06-17
US7534570B2 (en)	2009-05-19
EP2085487A1 (de)	2009-08-05
US7407749B2 (en)	2008-08-05
AU2002363259B2 (en)	2007-09-06
US20080113379A1 (en)	2008-05-15
DE50213489D1 (de)	2009-06-04
DE10154317A1 (de)	2003-05-15
ES2325121T3 (es)	2009-08-26
EP1438437A2 (de)	2004-07-21

Publication	Publication Date	Title
DK1438437T3 (da)	2009-07-20	Fremgangsmåde til påvisning af cytosin-methyleringer i immobiliserede DNA-pröver
DK1370691T3 (da)	2007-10-01	Fremgangsmåde til påvisning af cytosinmethyleringsmönstre med höj sensitivitet
WO2003000926A3 (de)	2003-10-09	Verfahren zum nachweis von cytosin-methylierung mit hoher sensitivität
ATE455870T1 (de)	2010-02-15	Oligonukleotide zur schnellen bestimmung von mikrobieller dns/rns
DK1185695T3 (da)	2006-11-06	Fremgangsmåde med höj kapacitet til analyse af methylering af DNA
DE69931646D1 (de)	2006-07-06	Multiplex genotypisierung unter verwendung von fluoreszenzmarkierten hybridisierungssonden
ATE395434T1 (de)	2008-05-15	Verfahren zur gleichzeitigen detektion von cytosin-methylierung und mutationen in dna proben
DK1468114T3 (da)	2008-09-01	LATE-PCR (Linear-After-The Exponential PCR)
WO2002046452A3 (de)	2003-09-18	Verfahren zur quantifizierung von cytosin-methylierungen in komplex amplifizierter genomischer dna
WO2001098528A3 (de)	2002-11-28	Verfahren zum nachweis von cytosin-methylierungen
ATE393239T1 (de)	2008-05-15	Verfahren zum nachweis von cytosin-methylierung in cpg-inseln
IS6206A (is)	2001-12-18	Aðferð til hlutfallslegrar skömmtunar á metýleringu á bösum af sýtósín-gerð í DNA sýnum
DK1654388T3 (da)	2007-10-29	Fremgangsmåde til påvisning af cytosinmethyleringer i DNA
DE50113630D1 (de)	2008-04-03	Verfahren zur detektion von cytosin-methylierung in dna proben
ATE368752T1 (de)	2007-08-15	Verfahren zum nachweis von cytosin-methylierungen
HUP0303377A2 (hu)	2004-01-28	Eljárás nukleinsav-molekulák kimutatására
ATE464397T1 (de)	2010-04-15	Cpg-amplicon und array-protokoll
WO2008068636A3 (en)	2008-10-16	Blocking agents comprising non-natural nucleic acids and detection methods using such blocking agents
DE50010776D1 (de)	2005-08-25	Verfahren zur unterscheidung von 5-position methylierungsänderungen
DE50214183D1 (de)	2010-03-11	Verfahren zum nachweis von in einer polymerase-kettenreaktion amplifizierten nukleinsäuremolekülen
WO2000065092A3 (en)	2001-07-19	Blocking non-specific binding of granulocytes in microorganism detection
EP1174521A3 (en)	2002-04-10	Method for detecting transcribed genomic DNA sequences