DK1490480T3 - Primitive og proksimale leverstamceller - Google Patents

Primitive og proksimale leverstamceller Download PDF

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DK1490480T3
DK1490480T3 DK03728245.6T DK03728245T DK1490480T3 DK 1490480 T3 DK1490480 T3 DK 1490480T3 DK 03728245 T DK03728245 T DK 03728245T DK 1490480 T3 DK1490480 T3 DK 1490480T3
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cells
liver
cell
stem cell
liver stem
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DK03728245.6T
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Lola Reid
Nicholas G Moss
Mark E Furth
John W Ludlow
Andrew T Bruce
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Univ North Carolina
Incara Cell Technologies Inc
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Claims (16)

1. Sammensætning, som omfatter en isoleret human primitiv leverstamcelle, hvor den isolerede primitive leverstamcelle ikke udtrykker alfa-føtoprotein, men udtrykker Ep-CAM, og hvor den isolerede primitive leverstamcelle er en precursor for en proksimal leverstamcelle, der er i stand til at være ophav til en hepatocytær progenitorcelle eller en biliær progenitorcelle, og idet den isolerede primitive leverstamcelle er en voksen enkeltcelle.
2. Sammensætning ifølge krav 1, hvor den isolerede humane primitive leverstamcelle udtrykker AC133 og albumin.
3. Sammensætning ifølge krav 2, hvor den isolerede humane primitive leverstamcelle udtrykker cytokeratin 8/18, cytokeratin 19, eller kombinationer deraf.
4. Sammensætning ifølge krav 3, hvor den isolerede humane primitive leverstamcelle udtrykker N-CAM, CAM5.2, c-kit, CD 146, eller kombinationer deraf.
5. Fremgangsmåde til isolering afen human primitiv leverprogenitorcelle, som kan være ophav til såvel hepatocytære progenitorceller som biliære progenitorceller, hvilken fremgangsmåde omfatter: (a) at tilvejebringe en suspension af celler, som stammer fra levervæv; (b) at overføre cellesuspensionen til en plastoverflade; (c) at dyrke cellerne under betingelser, der indbefatter anvendelse af et serumfrit medium, der omfatter en regulator af carbohydrat-metabolisme, en jernkilde og en membranproducerende faktor; og (d) at selektere de celler, der udtrykker Ep-CAM, og ikke udtrykker alfa-føtoprotein.
6. Fremgangsmåde ifølge krav 5, som yderligere omfatter selektion fra suspensionen af de celler, der udtrykker albumin, cytokeratin 19, AC133, cytokeratin 8/18, eller kombinationer deraf.
7. Fremgangsmåde ifølge krav 5, hvor levervævet er opnået fra et foster eller et nyfødt individ.
8. Fremgangsmåde ifølge krav 5, hvor levervævet er opnået fra et barn eller en voksen.
9. Fremgangsmåde ifølge krav 5 eller 6, som yderligere omfatter selektion fra suspensionen af de celler, der udtrykker N-CAM, CAM5.2, c-kit, CD146, eller kombinationer deraf.
10. Sammensætning ifølge et hvilket som helst af kravene 1 til 4, hvor den humane primitive leverstamcelle omfatter en eksogen nukleinsyre.
11. Isoleret human primitiv leverstamcelle, som ikke udtrykker alfa-føtoprotein, som udtrykker Ep-CAM, og som er i stand til at være ophav til såvel hepatocytære progenitorceller som biliære progenitorceller, idet den isolerede primitive leverstamcelle er en voksen enkeltcelle.
12. Isoleret human primitiv leverstamcelle ifølge krav 11, som yderligere udtrykker AC133 og albumin.
13. Isoleret human primitiv leverstamcelle ifølge krav 12, som yderligere udtrykker cytokeratin 8/18, cytokeratin 19, eller kombinationer deraf.
14. Isoleret human primitiv leverstamcelle ifølge krav 12, som yderligere udtrykker N-CAM, CAM5.2, c-kit, CD146, eller kombinationer deraf.
15. Isoleret human primitiv leverstamcelle ifølge et hvilket som helst af kravene 12 til 14, som yderligere omfatter en eksogen nukleinsyre.
16. Fremgangsmåde til fremstilling af et lægemiddel til behandling af leverdysfunktion eller -sygdom, hvilken fremgangsmåde omfatter blanding af en effektiv mængde af den humane primitive leverstamcelle ifølge et hvilket som helst af kravene 11 til 15 med en farmaceutisk acceptabel bærer, en farmaceutisk acceptabel excipiens, eller et farmaceutisk acceptabelt fortyndingsmiddel.
DK03728245.6T 2002-03-15 2003-03-14 Primitive og proksimale leverstamceller DK1490480T3 (da)

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US36536102P 2002-03-15 2002-03-15
PCT/US2003/007852 WO2003078588A2 (en) 2002-03-15 2003-03-14 Primitive and proximal hepatic stem cells

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EP (2) EP3023491A1 (da)
JP (2) JP4723811B2 (da)
KR (1) KR101119878B1 (da)
CN (3) CN1742082B (da)
AU (2) AU2003233399B2 (da)
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EP1490480A4 (en) 2006-06-14
JP2011087591A (ja) 2011-05-06
EP1490480A2 (en) 2004-12-29
JP2005520513A (ja) 2005-07-14
CA2479510A1 (en) 2003-09-25
US20140302601A1 (en) 2014-10-09
JP4723811B2 (ja) 2011-07-13
IL164048A0 (en) 2005-12-18
PL372619A1 (en) 2005-07-25
AU2008202656B8 (en) 2011-12-15
CN1742082A (zh) 2006-03-01
CA2479510C (en) 2019-10-29
WO2003078588A3 (en) 2004-04-01
HK1209783A1 (en) 2016-04-08
AU2008202656A8 (en) 2011-12-15
MXPA04009031A (es) 2005-12-12
CN1742082B (zh) 2015-01-21
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