DK1495128T3 - Forstærket proteinekspression i Bacillus - Google Patents

Forstærket proteinekspression i Bacillus

Info

Publication number
DK1495128T3
DK1495128T3 DK03726142.7T DK03726142T DK1495128T3 DK 1495128 T3 DK1495128 T3 DK 1495128T3 DK 03726142 T DK03726142 T DK 03726142T DK 1495128 T3 DK1495128 T3 DK 1495128T3
Authority
DK
Denmark
Prior art keywords
bacillus
protein expression
enhanced protein
enhanced
expression
Prior art date
Application number
DK03726142.7T
Other languages
English (en)
Inventor
Carole Harbison
M Harunur Rashid
Eugenio Ferrari
Walter Weyler
Original Assignee
Genencor Int
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Genencor Int filed Critical Genencor Int
Application granted granted Critical
Publication of DK1495128T3 publication Critical patent/DK1495128T3/da

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/75—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Bacillus
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/32—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Bacillus (G)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C12N1/205—Bacterial isolates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • C12N9/54—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea bacteria being Bacillus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/07—Bacillus
    • C12R2001/125—Bacillus subtilis ; Hay bacillus; Grass bacillus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Gastroenterology & Hepatology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
DK03726142.7T 2002-03-29 2003-03-28 Forstærket proteinekspression i Bacillus DK1495128T3 (da)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US36894902P 2002-03-29 2002-03-29
US36885802P 2002-03-29 2002-03-29
US37634302P 2002-04-29 2002-04-29
PCT/US2003/009585 WO2003083125A1 (en) 2002-03-29 2003-03-28 Ehanced protein expression in bacillus

Publications (1)

Publication Number Publication Date
DK1495128T3 true DK1495128T3 (da) 2014-08-11

Family

ID=28678930

Family Applications (2)

Application Number Title Priority Date Filing Date
DK03726142.7T DK1495128T3 (da) 2002-03-29 2003-03-28 Forstærket proteinekspression i Bacillus
DK11150877.6T DK2339016T3 (da) 2002-03-29 2003-03-28 Øget produktion af subtilisiner

Family Applications After (1)

Application Number Title Priority Date Filing Date
DK11150877.6T DK2339016T3 (da) 2002-03-29 2003-03-28 Øget produktion af subtilisiner

Country Status (7)

Country Link
US (4) US8124399B2 (da)
EP (5) EP1495128B1 (da)
JP (3) JP4680512B2 (da)
AU (1) AU2003228393A1 (da)
CA (2) CA2915148C (da)
DK (2) DK1495128T3 (da)
WO (1) WO2003083125A1 (da)

Families Citing this family (58)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1495128B1 (en) * 2002-03-29 2014-05-07 Genencor International, Inc. Ehanced protein expression in bacillus
JP4336184B2 (ja) * 2003-11-07 2009-09-30 花王株式会社 組換え微生物
DK2664670T3 (da) 2003-12-03 2015-07-27 Danisco Us Inc Perhydrolase
WO2006033668A2 (en) * 2004-04-09 2006-03-30 Genencor International, Inc. Pcka modifications and enhanced protein expression in bacillus
JP2005348641A (ja) * 2004-06-09 2005-12-22 Kao Corp 宿主微生物
WO2007043327A1 (ja) * 2005-10-13 2007-04-19 Kao Corporation 新規枯草菌変異株
DE102006004871A1 (de) * 2006-02-02 2007-08-09 Wacker Chemie Ag Mikroorganismenstamm zur Produktion von rekombinanten Proteinen
ES2401607T3 (es) * 2006-04-24 2013-04-23 Ajinomoto Co., Inc. Bacteria que puede producir una sustancia de purina y procedimiento para producir una sustancia de purina
ATE443760T1 (de) * 2006-05-31 2009-10-15 Novozymes As Chloramphenicolresistenz-selektion bei bacillus licheniformis
WO2008112258A2 (en) * 2007-03-12 2008-09-18 Danisco Us Inc. Modified proteases
RU2010121967A (ru) * 2007-10-31 2011-12-10 Медиммун, Ллк (Us) Белковые каркасные структуры
MX2010010310A (es) * 2008-03-28 2010-10-08 Danisco Us Inc Metodo para amplificar un locus en una celula bacteriana.
CA2796010C (en) 2010-04-13 2020-05-12 Medimmune, Llc Trail r2-specific multimeric scaffolds
KR20220116585A (ko) 2011-10-11 2022-08-23 비엘라 바이오, 인크. Cd40l 특이적 tn3 유래 스카폴드 및 이의 사용 방법
WO2013138351A1 (en) 2012-03-13 2013-09-19 Board Of Trustees Of The University Of Arkansas Separatome-based protein expression and purification platform
US8945837B2 (en) 2013-03-15 2015-02-03 American Sterilizer Company Sterilization indicator including a simplified genetically engineered biological indicator
EP2796556A1 (en) 2013-04-25 2014-10-29 Rijksuniversiteit Groningen Improved means and methods for expressing recombinant proteins
US11130969B2 (en) 2013-07-12 2021-09-28 Novozymes, A/S Direct transfer of polynucleotides between genomes
WO2015042105A1 (en) 2013-09-17 2015-03-26 Board Of Trustees Of The University Of Arkansas E. coli separatome-based protein expression and purification platform
JP2015156844A (ja) * 2014-02-25 2015-09-03 花王株式会社 枯草菌変異株及びそれを用いたジピコリン酸の製造方法
JP6437737B2 (ja) * 2014-05-09 2018-12-12 花王株式会社 枯草菌変異株及びそれを用いたポリ−γ−グルタミン酸の製造方法
JP6527669B2 (ja) * 2014-05-12 2019-06-05 花王株式会社 枯草菌変異株及びそれを用いたジピコリン酸の製造方法
IL315468A (en) 2014-09-17 2024-11-01 Spogen Biotech Inc Fusion proteins, recombinant bacteria, and methods for using recombinant bacteria
US10683528B2 (en) 2014-12-16 2020-06-16 Danisco Us Inc Enhanced protein expression
WO2018053058A1 (en) 2016-09-14 2018-03-22 Danisco Us Inc. Lignocellulosic biomass fermentation-based processes
EP3585910B1 (en) 2017-02-24 2024-03-20 Danisco US Inc. Compositions and methods for increased protein production in bacillus licheniformis
FI3607097T3 (fi) * 2017-04-07 2023-09-11 Dupont Nutrition Biosci Aps BEETA-GALAKTOSIDAASEJA JA LAKTAASEJA ILMAN p-NITROBENTSYYLIESTERASISIVUAKTIIVISUUTTA TUOTTAVIA BACILLUS-ISÄNTÄSOLUJA
US11879127B2 (en) 2017-08-23 2024-01-23 Danisco Us Inc. Methods and compositions for efficient genetic modifications of Bacillus licheniformis strains
US20210032639A1 (en) 2017-09-13 2021-02-04 Danisco Us Inc. Modified 5'-untranslated region (utr) sequences for increased protein production in bacillus
CA3076491A1 (en) 2017-09-20 2019-03-28 Spogen Biotech Inc. Fusion proteins, recombinant bacteria, and exosporium fragments for plant health
CN111670244B (zh) 2018-01-03 2023-08-01 丹尼斯科美国公司 突变的和遗传修饰的芽孢杆菌属细胞及其用于增加蛋白质产生的方法
CN109355303B (zh) * 2018-11-14 2022-05-06 天津大学 抑制和/或敲除基因在提高单克隆抗体的表达量中的应用
US11781147B2 (en) 2018-11-28 2023-10-10 Danisco Us Inc. Promoter sequences and methods thereof for enhanced protein production in Bacillus cells
CN109868253B (zh) * 2019-01-11 2020-03-13 齐鲁工业大学 抑制菌体自溶的地衣芽孢杆菌工程菌及其构建方法和应用
KR20220041928A (ko) 2019-08-14 2022-04-01 다니스코 유에스 인크. 바실러스 리체니포르미스에서 단백질 생산을 증가시키기 위한 조성물 및 방법
WO2021086606A1 (en) 2019-10-28 2021-05-06 Danisco Us Inc Microbial host cells for the production of heterologous cyanuric acid hydrolases and biuret hydrolases
JP7656603B2 (ja) 2019-11-11 2025-04-03 ダニスコ・ユーエス・インク バチルス属（ｂａｃｉｌｌｕｓ）細胞における強化されたタンパク質産生のための組成物及び方法
JP7787077B2 (ja) 2020-01-15 2025-12-16 ダニスコ・ユーエス・インク バチルス・リケニフォルミス（ｂａｃｉｌｌｕｓ ｌｉｃｈｅｎｉｆｏｒｍｉｓ）における強化したタンパク質産生のための組成物及び方法
CN116897160A (zh) 2021-02-22 2023-10-17 丹尼斯科美国公司 在色素缺陷型芽孢杆菌属细胞中产生目的蛋白的方法和组合物
CN117769597A (zh) 2021-05-24 2024-03-26 丹尼斯科美国公司 用于增强芽孢杆菌属细胞中蛋白质产生的组合物和方法
US20240294894A1 (en) 2021-06-24 2024-09-05 Basf Se Improved bacillus host cell with altered rema/remb protein
CN117813317A (zh) 2021-06-24 2024-04-02 巴斯夫欧洲公司 改良的芽孢杆菌生产宿主
EP4387982A2 (en) 2021-08-20 2024-06-26 Danisco US Inc. Methods and compositions for enhanced protein production in bacillus cells
KR20240099301A (ko) 2021-11-16 2024-06-28 다니스코 유에스 인크. 바실러스 세포에서 단백질 생산을 향상시키기 위한 조성물 및 방법
US20250197910A1 (en) 2022-03-24 2025-06-19 Danisco Us Inc. Compositions and methods for industrial scale production and recovery of lectins
KR20240167690A (ko) 2022-04-01 2024-11-27 다니스코 유에스 인크. 그람 양성 박테리아 세포에서 단백질 생산을 강화하는 프로-영역 돌연변이
WO2023245191A2 (en) * 2022-06-17 2023-12-21 Motif Foodworks, Inc. Inducible cell lysis system
WO2024040043A1 (en) 2022-08-16 2024-02-22 International N&H Denmark Aps Expression systems for phosphatases
JP2024028032A (ja) * 2022-08-19 2024-03-01 富士電機株式会社 炭化珪素半導体装置および炭化珪素半導体装置の製造方法
US20260092297A1 (en) 2022-09-02 2026-04-02 Danisco Us Inc. Novel promoter and 5'-untranslated region mutations enhancing protein production in gram-positive cells
CN119997966A (zh) 2022-09-20 2025-05-13 国际N&H丹麦有限公司 变体抗病毒多肽
EP4608972A1 (en) 2022-10-24 2025-09-03 Danisco US Inc. Compositions and methods for enhanced protein production in bacillus cells
WO2025007098A1 (en) 2023-06-30 2025-01-02 Danisco Us Inc. Recombinant fungal cells and methods thereof for industrial scale production of lectins
CN121889413A (zh) 2023-08-09 2026-04-17 丹尼斯科美国公司 用于增强革兰氏阳性细菌细胞中的蛋白质产生的组合物和方法
WO2025101486A1 (en) 2023-11-09 2025-05-15 Danisco Us Inc. Methods and compositions for enhanced protein production in bacillus cells
WO2025262181A1 (en) 2024-06-19 2025-12-26 Fresenius Kabi Ipsum S.R.L. Method to improve the producibility of a peptiligase enzyme
WO2026030345A1 (en) 2024-07-29 2026-02-05 Danisco Us Inc. Signal and pro-region sequence variants for enhanced protease production in bacillus cells
WO2026090396A1 (en) 2024-10-24 2026-04-30 Danisco Us Inc. Compositions and methods for enhanced protein production in gram‑positive bacterial cells

Family Cites Families (41)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4302544A (en) * 1979-10-15 1981-11-24 University Of Rochester Asporogenous mutant of B. subtilis for use as host component of HV1 system
US4450235A (en) 1982-04-21 1984-05-22 Cpc International Inc. Asporogenic mutant of bacillus subtilis useful as a host in a host-vector system
US5310675A (en) * 1983-06-24 1994-05-10 Genencor, Inc. Procaryotic carbonyl hydrolases
US4760025A (en) 1984-05-29 1988-07-26 Genencor, Inc. Modified enzymes and methods for making same
NZ208612A (en) * 1983-06-24 1991-09-25 Genentech Inc Method of producing "procaryotic carbonyl hydrolases" containing predetermined, site specific mutations
DE3480411D1 (en) 1983-07-06 1989-12-14 Gist Brocades Nv Molecular cloning and expression in industrial microorganism species
US5264366A (en) 1984-05-29 1993-11-23 Genencor, Inc. Protease deficient bacillus
US5763257A (en) 1984-05-29 1998-06-09 Genencor International, Inc. Modified subtilisins having amino acid alterations
US4965188A (en) 1986-08-22 1990-10-23 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences using a thermostable enzyme
US4683195A (en) 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US4683202A (en) * 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
WO1987004461A1 (en) 1986-01-15 1987-07-30 Amgen THERMALLY STABLE AND pH STABLE SUBTILISIN ANALOGS AND METHOD FOR PRODUCTION THEREOF
US4980288A (en) 1986-02-12 1990-12-25 Genex Corporation Subtilisin with increased thermal stability
GB8608194D0 (en) 1986-04-03 1986-05-08 Massey Ferguson Services Nv Valve control system
JPH044149B2 (da) * 1986-06-19 1992-01-27
US5322770A (en) 1989-12-22 1994-06-21 Hoffman-Laroche Inc. Reverse transcription with thermostable DNA polymerases - high temperature reverse transcription
KR0127560B1 (ko) 1987-02-27 1997-12-29 마가렛트 에이.호른 단백질 분해 효소 코드화 유전자의 분자클로닝 및 발현
US4914031A (en) 1987-04-10 1990-04-03 Amgen, Inc. Subtilisin analogs
DK6488D0 (da) * 1988-01-07 1988-01-07 Novo Industri As Enzymer
CN1056187C (zh) 1988-02-11 2000-09-06 金克克国际有限公司 新的蛋白水解酶及其在洗涤剂中的应用
CA2003078A1 (en) * 1988-11-18 1990-05-18 Biotechnica International, Inc. Protease deletion
US5665587A (en) * 1989-06-26 1997-09-09 Novo Nordisk A/S Modified subtilisins and detergent compositions containing same
US5023171A (en) * 1989-08-10 1991-06-11 Mayo Foundation For Medical Education And Research Method for gene splicing by overlap extension using the polymerase chain reaction
DK0414297T3 (da) 1989-08-11 1997-07-07 Genencor Int Effektiv fremstilling af mutantproteaser
US5585253A (en) * 1989-11-27 1996-12-17 The Regents Of The University Of California Extracellular serine protease and a Bacillus subtilis alkaline neutral an serine protease mutant strain
IE892893A1 (en) * 1990-03-11 1991-09-25 Charles O Kane Phage-like bacteriocins
DK97190D0 (da) * 1990-04-19 1990-04-19 Novo Nordisk As Oxidationsstabile detergentenzymer
US5482849A (en) * 1990-12-21 1996-01-09 Novo Nordisk A/S Subtilisin mutants
KR100230613B1 (ko) * 1991-04-26 1999-12-01 마가렛 에이.혼 간균내 유전자 발현
ES2121014T3 (es) 1991-05-01 1998-11-16 Novo Nordisk As Enzimas estabilizadas y composiciones detergentes.
DE4411223A1 (de) 1994-03-31 1995-10-05 Solvay Enzymes Gmbh & Co Kg Verwendung alkalischer Proteasen in gewerblichen Textilwaschverfahren
WO1999003984A2 (en) * 1997-07-15 1999-01-28 Genencor International, Inc. Proteases from gram-positive organisms
AR016969A1 (es) 1997-10-23 2001-08-01 Procter & Gamble VARIANTE DE PROTEASA, ADN, VECTOR DE EXPRESIoN, MICROORGANISMO HUESPED, COMPOSICIoN DE LIMPIEZA, ALIMENTO PARA ANIMALES Y COMPOSICIoN PARA TRATAR UN TEXTIL
US6528255B1 (en) * 1997-12-30 2003-03-04 Genencor International, Inc. Proteases from gram positive organisms
US6835550B1 (en) 1998-04-15 2004-12-28 Genencor International, Inc. Mutant proteins having lower allergenic response in humans and methods for constructing, identifying and producing such proteins
US6509185B1 (en) * 2000-01-07 2003-01-21 Genencor International, Inc. Mutant aprE promotor
EP1290194B1 (en) * 2000-06-14 2010-05-26 Genencor International, Inc. Production of secreted polypeptides
US20020182734A1 (en) * 2000-08-11 2002-12-05 Diaz-Torres Maria R. Bacillus transformation, transformants and mutant libraries
DE10043331A1 (de) 2000-09-02 2002-03-14 Degussa Neue für das sigD-Gen kodierende Nukleotidsequenzen
DK1354056T3 (da) * 2000-10-10 2015-02-23 Danisco Us Inc Forbedret sekretion af et polypeptid ved hjælp af en mikroorganisme
EP1495128B1 (en) * 2002-03-29 2014-05-07 Genencor International, Inc. Ehanced protein expression in bacillus

Also Published As

Publication number Publication date
EP3023501A1 (en) 2016-05-25
EP2339017A3 (en) 2011-10-12
EP1495128A1 (en) 2005-01-12
US20120183995A1 (en) 2012-07-19
US20060073559A1 (en) 2006-04-06
EP2339017A2 (en) 2011-06-29
EP2339016A2 (en) 2011-06-29
US9175294B2 (en) 2015-11-03
US8124399B2 (en) 2012-02-28
WO2003083125A1 (en) 2003-10-09
CA2915148A1 (en) 2003-10-09
AU2003228393A1 (en) 2003-10-13
EP2339016A3 (en) 2011-11-16
JP2011004759A (ja) 2011-01-13
US20160168582A1 (en) 2016-06-16
JP2009225804A (ja) 2009-10-08
EP1495128B1 (en) 2014-05-07
EP1495128A4 (en) 2006-11-02
US8383366B2 (en) 2013-02-26
DK2339016T3 (da) 2017-01-23
US9617549B2 (en) 2017-04-11
EP2339016B8 (en) 2016-01-27
CA2915148C (en) 2018-03-13
EP2341070A2 (en) 2011-07-06
CA2480277A1 (en) 2003-10-09
JP5170793B2 (ja) 2013-03-27
EP2339016B1 (en) 2015-11-25
JP5007319B2 (ja) 2012-08-22
EP2341070A3 (en) 2011-10-19
JP2005521407A (ja) 2005-07-21
JP4680512B2 (ja) 2011-05-11
CA2480277C (en) 2016-02-09
US20140011234A1 (en) 2014-01-09

Similar Documents

Publication Publication Date Title
EP1495128A4 (en) IMPROVED PROTEIN EXPRESSION IN BACILLUS
DK1490404T3 (da) Laktoferrin
EP1478761A4 (en) DEGRADATION OF PESTICIDES AND HYDROPHOBIC ESTERIC TOXINS
NO20053512D0 (no) IL-8-liknende proteiner
NO20052608D0 (no) Midkinlignende protein
ATA522002A (de) Vmp-bindungsprotein
DE10255292B4 (de) Armlehne
UA6902S (uk) Стілець
UA7060S (uk) Стілець
GB0207141D0 (en) Modified protein
GB0216601D0 (en) Modified protein
GB0321300D0 (en) Protein structure and uses thereof
GB0228538D0 (en) Protein structure and uses thereof
CA94765S (fr) Bouteille
UA6785S (uk) Пляшка
UA6825S (uk) Пляшка
UA6295S (uk) Пляшка
UA6296S (uk) Пляшка
UA6482S (uk) Пляшка
UA6443S (uk) Пляшка
UA6077S (uk) Пляшка
UA6655S (uk) Пляшка
UA6631S (uk) Пляшка
UA6010S (uk) Пляшка
UA6285S (uk) Пляшка