DK1654360T3 - Amplificering fremgangsmåde - Google Patents
Amplificering fremgangsmådeInfo
- Publication number
- DK1654360T3 DK1654360T3 DK04743697T DK04743697T DK1654360T3 DK 1654360 T3 DK1654360 T3 DK 1654360T3 DK 04743697 T DK04743697 T DK 04743697T DK 04743697 T DK04743697 T DK 04743697T DK 1654360 T3 DK1654360 T3 DK 1654360T3
- Authority
- DK
- Denmark
- Prior art keywords
- amplification procedure
- amplification
- procedure
- Prior art date
Links
- 230000003321 amplification Effects 0.000 title 1
- 238000000034 method Methods 0.000 title 1
- 238000003199 nucleic acid amplification method Methods 0.000 title 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1096—Processes for the isolation, preparation or purification of DNA or RNA cDNA Synthesis; Subtracted cDNA library construction, e.g. RT, RT-PCR
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/26—Preparation of nitrogen-containing carbohydrates
- C12P19/28—N-glycosides
- C12P19/30—Nucleotides
- C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Molecular Biology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Biomedical Technology (AREA)
- General Health & Medical Sciences (AREA)
- Plant Pathology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Crystallography & Structural Chemistry (AREA)
- Bioinformatics & Computational Biology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GBGB0319332.3A GB0319332D0 (en) | 2003-08-16 | 2003-08-16 | Amplification |
| PCT/GB2004/003486 WO2005019452A1 (en) | 2003-08-16 | 2004-08-12 | Amplification method |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| DK1654360T3 true DK1654360T3 (da) | 2008-02-25 |
Family
ID=28052667
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DK04743697T DK1654360T3 (da) | 2003-08-16 | 2004-08-12 | Amplificering fremgangsmåde |
Country Status (8)
| Country | Link |
|---|---|
| US (1) | US20070105090A1 (da) |
| EP (1) | EP1654360B1 (da) |
| JP (1) | JP2007502610A (da) |
| DE (1) | DE602004010273T2 (da) |
| DK (1) | DK1654360T3 (da) |
| ES (1) | ES2295886T3 (da) |
| GB (1) | GB0319332D0 (da) |
| WO (1) | WO2005019452A1 (da) |
Families Citing this family (50)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR101606929B1 (ko) | 2008-04-30 | 2016-03-29 | 포항공과대학교 산학협력단 | 모세관 전기영동에 의한 다중 mRNA 또는 16S rRNA분석을 위한 주형 변형방법 및 이를 이용한 미생물검출방법 |
| EP3002337B1 (en) | 2009-03-30 | 2018-10-24 | Illumina, Inc. | Gene expression analysis in single cells |
| US8835358B2 (en) | 2009-12-15 | 2014-09-16 | Cellular Research, Inc. | Digital counting of individual molecules by stochastic attachment of diverse labels |
| CA2865575C (en) | 2012-02-27 | 2024-01-16 | Cellular Research, Inc. | Compositions and kits for molecular counting |
| US9410173B2 (en) | 2012-10-24 | 2016-08-09 | Clontech Laboratories, Inc. | Template switch-based methods for producing a product nucleic acid |
| EP3611262B1 (en) * | 2013-03-15 | 2020-11-11 | Lineage Biosciences, Inc. | Methods of sequencing the immune repertoire |
| US9567645B2 (en) | 2013-08-28 | 2017-02-14 | Cellular Research, Inc. | Massively parallel single cell analysis |
| CA2923812C (en) | 2013-10-17 | 2023-10-17 | Clontech Laboratories, Inc. | Methods for adding adapters to nucleic acids and compositions for practicing the same |
| US9719136B2 (en) | 2013-12-17 | 2017-08-01 | Takara Bio Usa, Inc. | Methods for adding adapters to nucleic acids and compositions for practicing the same |
| EP3259371B1 (en) | 2015-02-19 | 2020-09-02 | Becton, Dickinson and Company | High-throughput single-cell analysis combining proteomic and genomic information |
| CN107208158B (zh) | 2015-02-27 | 2022-01-28 | 贝克顿迪金森公司 | 空间上可寻址的分子条形编码 |
| EP3835431B1 (en) | 2015-03-30 | 2022-11-02 | Becton, Dickinson and Company | Methods for combinatorial barcoding |
| US11390914B2 (en) | 2015-04-23 | 2022-07-19 | Becton, Dickinson And Company | Methods and compositions for whole transcriptome amplification |
| WO2016196229A1 (en) | 2015-06-01 | 2016-12-08 | Cellular Research, Inc. | Methods for rna quantification |
| JP6940484B2 (ja) | 2015-09-11 | 2021-09-29 | セルラー リサーチ, インコーポレイテッド | ライブラリー正規化のための方法および組成物 |
| EP3452614B1 (en) | 2016-05-02 | 2023-06-28 | Becton, Dickinson and Company | Accurate molecular barcoding |
| US10301677B2 (en) | 2016-05-25 | 2019-05-28 | Cellular Research, Inc. | Normalization of nucleic acid libraries |
| CN109074430B (zh) | 2016-05-26 | 2022-03-29 | 贝克顿迪金森公司 | 分子标记计数调整方法 |
| US10202641B2 (en) | 2016-05-31 | 2019-02-12 | Cellular Research, Inc. | Error correction in amplification of samples |
| US10640763B2 (en) | 2016-05-31 | 2020-05-05 | Cellular Research, Inc. | Molecular indexing of internal sequences |
| AU2017331459B2 (en) | 2016-09-26 | 2023-04-13 | Becton, Dickinson And Company | Measurement of protein expression using reagents with barcoded oligonucleotide sequences |
| ES2961580T3 (es) | 2017-01-13 | 2024-03-12 | Cellular Res Inc | Revestimiento hidrófilo de canales de fluidos |
| CN110382708A (zh) | 2017-02-01 | 2019-10-25 | 赛卢拉研究公司 | 使用阻断性寡核苷酸进行选择性扩增 |
| WO2018226293A1 (en) | 2017-06-05 | 2018-12-13 | Becton, Dickinson And Company | Sample indexing for single cells |
| WO2019126209A1 (en) | 2017-12-19 | 2019-06-27 | Cellular Research, Inc. | Particles associated with oligonucleotides |
| EP3788171B1 (en) | 2018-05-03 | 2023-04-05 | Becton, Dickinson and Company | High throughput multiomics sample analysis |
| EP3788170B1 (en) | 2018-05-03 | 2025-01-01 | Becton, Dickinson and Company | Molecular barcoding on opposite transcript ends |
| WO2020072380A1 (en) | 2018-10-01 | 2020-04-09 | Cellular Research, Inc. | Determining 5' transcript sequences |
| EP3877520B1 (en) | 2018-11-08 | 2025-03-19 | Becton, Dickson And Company | Whole transcriptome analysis of single cells using random priming |
| EP4745247A2 (en) | 2018-12-13 | 2026-05-20 | Becton Dickinson Co | SELECTIVE EXTENSION IN A SINGLE-CELL WHOLE-TRANSCRIPTOME ANALYSIS |
| WO2020150356A1 (en) | 2019-01-16 | 2020-07-23 | Becton, Dickinson And Company | Polymerase chain reaction normalization through primer titration |
| CN113574178B (zh) | 2019-01-23 | 2024-10-29 | 贝克顿迪金森公司 | 与抗体关联的寡核苷酸 |
| CN120099139A (zh) | 2019-02-14 | 2025-06-06 | 贝克顿迪金森公司 | 杂合体靶向和全转录物组扩增 |
| US11965208B2 (en) | 2019-04-19 | 2024-04-23 | Becton, Dickinson And Company | Methods of associating phenotypical data and single cell sequencing data |
| US20220228139A1 (en) * | 2019-05-21 | 2022-07-21 | Academia Sinica | Method for amplifying and detecting ribonucleic acid (rna) fragments |
| CN110241459A (zh) * | 2019-05-31 | 2019-09-17 | 南方医科大学南方医院 | 一种甄别样品间与独立样品自身交叉反应的免疫组库方法 |
| CN120099137A (zh) | 2019-07-22 | 2025-06-06 | 贝克顿迪金森公司 | 单细胞染色质免疫沉淀测序测定 |
| CN112501249B (zh) * | 2019-09-16 | 2024-01-26 | 深圳市真迈生物科技有限公司 | Rna文库的制备方法、测序方法和试剂盒 |
| EP4055160B1 (en) | 2019-11-08 | 2024-04-10 | Becton Dickinson and Company | Using random priming to obtain full-length v(d)j information for immune repertoire sequencing |
| WO2021146219A1 (en) | 2020-01-13 | 2021-07-22 | Becton, Dickinson And Company | Cell capture using du-containing oligonucleotides |
| EP4090763B1 (en) | 2020-01-13 | 2024-12-04 | Becton Dickinson and Company | Methods and compositions for quantitation of proteins and rna |
| ES2993319T3 (en) | 2020-01-29 | 2024-12-27 | Becton Dickinson Co | Barcoded wells for spatial mapping of single cells through sequencing |
| CN115151810A (zh) | 2020-02-25 | 2022-10-04 | 贝克顿迪金森公司 | 实现使用单细胞样品作为单色补偿对照的双特异性探针 |
| US11661625B2 (en) | 2020-05-14 | 2023-05-30 | Becton, Dickinson And Company | Primers for immune repertoire profiling |
| CN115803445A (zh) | 2020-06-02 | 2023-03-14 | 贝克顿迪金森公司 | 用于5撇基因表达测定的寡核苷酸和珠 |
| US11932901B2 (en) | 2020-07-13 | 2024-03-19 | Becton, Dickinson And Company | Target enrichment using nucleic acid probes for scRNAseq |
| WO2022026909A1 (en) | 2020-07-31 | 2022-02-03 | Becton, Dickinson And Company | Single cell assay for transposase-accessible chromatin |
| US11739443B2 (en) | 2020-11-20 | 2023-08-29 | Becton, Dickinson And Company | Profiling of highly expressed and lowly expressed proteins |
| US12392771B2 (en) | 2020-12-15 | 2025-08-19 | Becton, Dickinson And Company | Single cell secretome analysis |
| WO2024190788A1 (ja) * | 2023-03-13 | 2024-09-19 | 国立研究開発法人理化学研究所 | Rna鋳型rna増幅を利用したdnaライブラリの作成方法 |
Family Cites Families (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5545522A (en) * | 1989-09-22 | 1996-08-13 | Van Gelder; Russell N. | Process for amplifying a target polynucleotide sequence using a single primer-promoter complex |
| US5962271A (en) * | 1996-01-03 | 1999-10-05 | Cloutech Laboratories, Inc. | Methods and compositions for generating full-length cDNA having arbitrary nucleotide sequence at the 3'-end |
| US5932451A (en) * | 1997-11-19 | 1999-08-03 | Incyte Pharmaceuticals, Inc. | Method for unbiased mRNA amplification |
| WO2001073134A2 (en) * | 2000-03-28 | 2001-10-04 | The Government Of The United States Of America, As Represented By The Secretary, Department Of Health & Human Services, The National Institutes Of Health | Gene profiling arrays |
| US6465219B1 (en) * | 2000-08-07 | 2002-10-15 | Genemed Biotechnologies, Inc. | Polynucleotide pools enriched in either high-abundance or low-abundance sequences |
| CA2480525A1 (en) * | 2002-03-29 | 2003-10-09 | Nugen Technologies, Inc. | Single primer isothermal nucleic acid amplification-enhanced analyte detection and quantification |
-
2003
- 2003-08-16 GB GBGB0319332.3A patent/GB0319332D0/en not_active Ceased
-
2004
- 2004-08-12 WO PCT/GB2004/003486 patent/WO2005019452A1/en not_active Ceased
- 2004-08-12 JP JP2006523675A patent/JP2007502610A/ja active Pending
- 2004-08-12 DE DE602004010273T patent/DE602004010273T2/de not_active Expired - Fee Related
- 2004-08-12 ES ES04743697T patent/ES2295886T3/es not_active Expired - Lifetime
- 2004-08-12 DK DK04743697T patent/DK1654360T3/da active
- 2004-08-12 EP EP04743697A patent/EP1654360B1/en not_active Expired - Lifetime
- 2004-11-16 US US10/568,432 patent/US20070105090A1/en not_active Abandoned
Also Published As
| Publication number | Publication date |
|---|---|
| US20070105090A1 (en) | 2007-05-10 |
| DE602004010273D1 (de) | 2008-01-03 |
| DE602004010273T2 (de) | 2008-09-25 |
| EP1654360A1 (en) | 2006-05-10 |
| JP2007502610A (ja) | 2007-02-15 |
| WO2005019452A1 (en) | 2005-03-03 |
| ES2295886T3 (es) | 2008-04-16 |
| GB0319332D0 (en) | 2003-09-17 |
| EP1654360B1 (en) | 2007-11-21 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| DE602004010273D1 (de) | Amplifikationsmethode | |
| ATE363383T1 (de) | Schlingenware | |
| DE502004001683D1 (de) | Kabelführung | |
| DE602004021604D1 (de) | Ammensetzung | |
| AT502792A5 (de) | Düsenanpressvorrichtung | |
| DE602004004230D1 (de) | Klasse-D Verstärker | |
| SE0301107L (sv) | Kraftförstärkare | |
| DE502004000672D1 (de) | Pigmentpreparationen | |
| DE112004000620D2 (de) | Struktoguss | |
| DE10355350A8 (de) | Elektromodul | |
| ATA662003A (de) | Trink-mundstück | |
| DE60326234D1 (de) | Magnetronkathodenanordnung | |
| DE502004002978D1 (de) | N-biarylamide | |
| DE502004001629D1 (de) | Bremsenprüfstand | |
| DE502004003122D1 (de) | Hydrolager | |
| DE502004006563D1 (de) | Brieftaubenkonstatiersystem | |
| DE502004000518D1 (de) | Mitgängergabelhubwagen | |
| DE112004002227D2 (de) | Nietverarbeitungsgerät | |
| DE10394329D2 (de) | Mäanderstent | |
| ATE406350T1 (de) | Prolinylarylacetamide | |
| DE10329218B4 (de) | Hotflue | |
| DE502004002771D1 (de) | Umlenkhebel | |
| DE502004007251D1 (de) | En 2-arylpropenalen | |
| DE112004001561D2 (de) | Getriebene-Antreibseinheit | |
| DE502004004175D1 (de) | Verbrennungskraftmaschime |