DK1654360T3 - Amplificering fremgangsmåde - Google Patents

Amplificering fremgangsmåde

Info

Publication number
DK1654360T3
DK1654360T3 DK04743697T DK04743697T DK1654360T3 DK 1654360 T3 DK1654360 T3 DK 1654360T3 DK 04743697 T DK04743697 T DK 04743697T DK 04743697 T DK04743697 T DK 04743697T DK 1654360 T3 DK1654360 T3 DK 1654360T3
Authority
DK
Denmark
Prior art keywords
amplification procedure
amplification
procedure
Prior art date
Application number
DK04743697T
Other languages
English (en)
Inventor
Alexander Graham
Andrew Cassidy
Original Assignee
Astrazeneca Ab
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Astrazeneca Ab filed Critical Astrazeneca Ab
Application granted granted Critical
Publication of DK1654360T3 publication Critical patent/DK1654360T3/da

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1096—Processes for the isolation, preparation or purification of DNA or RNA cDNA Synthesis; Subtracted cDNA library construction, e.g. RT, RT-PCR
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
DK04743697T 2003-08-16 2004-08-12 Amplificering fremgangsmåde DK1654360T3 (da)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GBGB0319332.3A GB0319332D0 (en) 2003-08-16 2003-08-16 Amplification
PCT/GB2004/003486 WO2005019452A1 (en) 2003-08-16 2004-08-12 Amplification method

Publications (1)

Publication Number Publication Date
DK1654360T3 true DK1654360T3 (da) 2008-02-25

Family

ID=28052667

Family Applications (1)

Application Number Title Priority Date Filing Date
DK04743697T DK1654360T3 (da) 2003-08-16 2004-08-12 Amplificering fremgangsmåde

Country Status (8)

Country Link
US (1) US20070105090A1 (da)
EP (1) EP1654360B1 (da)
JP (1) JP2007502610A (da)
DE (1) DE602004010273T2 (da)
DK (1) DK1654360T3 (da)
ES (1) ES2295886T3 (da)
GB (1) GB0319332D0 (da)
WO (1) WO2005019452A1 (da)

Families Citing this family (50)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR101606929B1 (ko) 2008-04-30 2016-03-29 포항공과대학교 산학협력단 모세관 전기영동에 의한 다중 ｍＲＮＡ 또는 16Ｓ ｒＲＮＡ분석을 위한 주형 변형방법 및 이를 이용한 미생물검출방법
US20120010091A1 (en) * 2009-03-30 2012-01-12 Illumina, Inc. Gene expression analysis in single cells
US8835358B2 (en) 2009-12-15 2014-09-16 Cellular Research, Inc. Digital counting of individual molecules by stochastic attachment of diverse labels
EP3321378B1 (en) 2012-02-27 2021-11-10 Becton, Dickinson and Company Compositions for molecular counting
EP2912197B1 (en) * 2012-10-24 2019-08-07 Takara Bio USA, Inc. Template switch-based methods for producing a product nucleic acid
EP2970959B1 (en) * 2013-03-15 2018-05-30 Lineage Biosciences, Inc. Methods and compositions for tagging and analyzing samples
CN105705659B (zh) 2013-08-28 2019-11-29 贝克顿迪金森公司 大规模平行单细胞分析
JP6602294B2 (ja) 2013-10-17 2019-11-06 タカラ バイオ ユーエスエー， インコーポレイテッド アダプタを核酸に追加する方法、及びこの方法を実行するための組成物
US9719136B2 (en) 2013-12-17 2017-08-01 Takara Bio Usa, Inc. Methods for adding adapters to nucleic acids and compositions for practicing the same
EP3259371B1 (en) 2015-02-19 2020-09-02 Becton, Dickinson and Company High-throughput single-cell analysis combining proteomic and genomic information
WO2016138496A1 (en) 2015-02-27 2016-09-01 Cellular Research, Inc. Spatially addressable molecular barcoding
CN107406888A (zh) 2015-03-30 2017-11-28 赛卢拉研究公司 用于组合条形编码的方法和组合物
EP3286326B1 (en) 2015-04-23 2025-01-22 Becton, Dickinson and Company Method for whole transcriptome amplification
US11124823B2 (en) 2015-06-01 2021-09-21 Becton, Dickinson And Company Methods for RNA quantification
WO2017044574A1 (en) 2015-09-11 2017-03-16 Cellular Research, Inc. Methods and compositions for nucleic acid library normalization
EP3452614B1 (en) 2016-05-02 2023-06-28 Becton, Dickinson and Company Accurate molecular barcoding
US10301677B2 (en) 2016-05-25 2019-05-28 Cellular Research, Inc. Normalization of nucleic acid libraries
WO2017205691A1 (en) 2016-05-26 2017-11-30 Cellular Research, Inc. Molecular label counting adjustment methods
US10640763B2 (en) 2016-05-31 2020-05-05 Cellular Research, Inc. Molecular indexing of internal sequences
US10202641B2 (en) 2016-05-31 2019-02-12 Cellular Research, Inc. Error correction in amplification of samples
IL265478B2 (en) 2016-09-26 2024-10-01 Becton Dickinson Co Measurement of protein expression using reagents with barcoded oligonucleotide sequences
WO2018132610A1 (en) 2017-01-13 2018-07-19 Cellular Research, Inc. Hydrophilic coating of fluidic channels
US11319583B2 (en) 2017-02-01 2022-05-03 Becton, Dickinson And Company Selective amplification using blocking oligonucleotides
JP7536450B2 (ja) 2017-06-05 2024-08-20 ベクトン・ディキンソン・アンド・カンパニー 単一細胞用のサンプルインデックス付加
CN111492068B (zh) 2017-12-19 2025-03-21 贝克顿迪金森公司 与寡核苷酸相关联的颗粒
CA3097976A1 (en) 2018-05-03 2019-11-07 Becton, Dickinson And Company High throughput multiomics sample analysis
EP3788170B1 (en) 2018-05-03 2025-01-01 Becton, Dickinson and Company Molecular barcoding on opposite transcript ends
EP3861134B1 (en) 2018-10-01 2024-09-04 Becton, Dickinson and Company Determining 5' transcript sequences
EP3877520B1 (en) 2018-11-08 2025-03-19 Becton, Dickson And Company Whole transcriptome analysis of single cells using random priming
EP3894552B1 (en) 2018-12-13 2026-01-28 Becton, Dickinson and Company Selective extension in single cell whole transcriptome analysis
WO2020150356A1 (en) 2019-01-16 2020-07-23 Becton, Dickinson And Company Polymerase chain reaction normalization through primer titration
EP3914728B1 (en) 2019-01-23 2023-04-05 Becton, Dickinson and Company Oligonucleotides associated with antibodies
CN113454234B (zh) 2019-02-14 2025-03-18 贝克顿迪金森公司 杂合体靶向和全转录物组扩增
US11965208B2 (en) 2019-04-19 2024-04-23 Becton, Dickinson And Company Methods of associating phenotypical data and single cell sequencing data
US20220228139A1 (en) * 2019-05-21 2022-07-21 Academia Sinica Method for amplifying and detecting ribonucleic acid (rna) fragments
CN110241459A (zh) * 2019-05-31 2019-09-17 南方医科大学南方医院 一种甄别样品间与独立样品自身交叉反应的免疫组库方法
WO2021016239A1 (en) 2019-07-22 2021-01-28 Becton, Dickinson And Company Single cell chromatin immunoprecipitation sequencing assay
CN112501249B (zh) * 2019-09-16 2024-01-26 深圳市真迈生物科技有限公司 Rna文库的制备方法、测序方法和试剂盒
EP4055160B1 (en) 2019-11-08 2024-04-10 Becton Dickinson and Company Using random priming to obtain full-length v(d)j information for immune repertoire sequencing
CN115244184A (zh) 2020-01-13 2022-10-25 贝克顿迪金森公司 用于定量蛋白和rna的方法和组合物
CN115279919A (zh) 2020-01-13 2022-11-01 贝克顿迪金森公司 使用含有du的寡核苷酸的细胞捕获
WO2021155057A1 (en) 2020-01-29 2021-08-05 Becton, Dickinson And Company Barcoded wells for spatial mapping of single cells through sequencing
US12153043B2 (en) 2020-02-25 2024-11-26 Becton, Dickinson And Company Bi-specific probes to enable the use of single-cell samples as single color compensation control
WO2021231779A1 (en) 2020-05-14 2021-11-18 Becton, Dickinson And Company Primers for immune repertoire profiling
CN115803445A (zh) 2020-06-02 2023-03-14 贝克顿迪金森公司 用于5撇基因表达测定的寡核苷酸和珠
US11932901B2 (en) 2020-07-13 2024-03-19 Becton, Dickinson And Company Target enrichment using nucleic acid probes for scRNAseq
WO2022026909A1 (en) 2020-07-31 2022-02-03 Becton, Dickinson And Company Single cell assay for transposase-accessible chromatin
WO2022109343A1 (en) 2020-11-20 2022-05-27 Becton, Dickinson And Company Profiling of highly expressed and lowly expressed proteins
CN116685850A (zh) 2020-12-15 2023-09-01 贝克顿迪金森公司 单细胞分泌组分析
WO2024190788A1 (ja) * 2023-03-13 2024-09-19 国立研究開発法人理化学研究所 Ｒｎａ鋳型ｒｎａ増幅を利用したｄｎａライブラリの作成方法

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5545522A (en) * 1989-09-22 1996-08-13 Van Gelder; Russell N. Process for amplifying a target polynucleotide sequence using a single primer-promoter complex
US5962271A (en) * 1996-01-03 1999-10-05 Cloutech Laboratories, Inc. Methods and compositions for generating full-length cDNA having arbitrary nucleotide sequence at the 3'-end
US5932451A (en) * 1997-11-19 1999-08-03 Incyte Pharmaceuticals, Inc. Method for unbiased mRNA amplification
WO2001073134A2 (en) * 2000-03-28 2001-10-04 The Government Of The United States Of America, As Represented By The Secretary, Department Of Health & Human Services, The National Institutes Of Health Gene profiling arrays
US6465219B1 (en) * 2000-08-07 2002-10-15 Genemed Biotechnologies, Inc. Polynucleotide pools enriched in either high-abundance or low-abundance sequences
EP1490514A4 (en) * 2002-03-29 2008-12-17 Nugen Technologies Inc THROUGH ISOTHERME SINGLE PRIMER NUCLEIC ACID AMPLIFICATION IMPROVED DETECTION AND QUANTIFICATION OF ANALYTES

Also Published As

Publication number Publication date
WO2005019452A1 (en) 2005-03-03
GB0319332D0 (en) 2003-09-17
US20070105090A1 (en) 2007-05-10
DE602004010273T2 (de) 2008-09-25
EP1654360A1 (en) 2006-05-10
EP1654360B1 (en) 2007-11-21
JP2007502610A (ja) 2007-02-15
ES2295886T3 (es) 2008-04-16
DE602004010273D1 (de) 2008-01-03

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