DK1681355T3 - Sekvensspecifik DNA-rekombination i eukaryote celler - Google Patents

Sekvensspecifik DNA-rekombination i eukaryote celler

Info

Publication number
DK1681355T3
DK1681355T3 DK06008807T DK06008807T DK1681355T3 DK 1681355 T3 DK1681355 T3 DK 1681355T3 DK 06008807 T DK06008807 T DK 06008807T DK 06008807 T DK06008807 T DK 06008807T DK 1681355 T3 DK1681355 T3 DK 1681355T3
Authority
DK
Denmark
Prior art keywords
sequence
derivatives
eukaryotic cells
specific dna
dna recombination
Prior art date
Application number
DK06008807T
Other languages
English (en)
Inventor
Peter Droege
Nicole Christ
Elke Lorbach
Original Assignee
Peter Droege
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Peter Droege filed Critical Peter Droege
Application granted granted Critical
Publication of DK1681355T3 publication Critical patent/DK1681355T3/da

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P3/00—Drugs for disorders of the metabolism
    • A61P3/06—Antihyperlipidemics
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
    • C12N15/907—Stable introduction of foreign DNA into chromosome using homologous recombination in mammalian cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P17/00—Drugs for dermatological disorders
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P21/00—Drugs for disorders of the muscular or neuromuscular system
    • A61P21/04—Drugs for disorders of the muscular or neuromuscular system for myasthenia gravis
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P37/00—Drugs for immunological or allergic disorders
    • A61P37/02—Immunomodulators
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P7/00—Drugs for disorders of the blood or the extracellular fluid
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K48/00—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2800/00—Nucleic acids vectors
    • C12N2800/30—Vector systems comprising sequences for excision in presence of a recombinase, e.g. loxP or FRT

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Mycology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Medicinal Chemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Hematology (AREA)
  • Immunology (AREA)
  • Diabetes (AREA)
  • Cell Biology (AREA)
  • Physical Education & Sports Medicine (AREA)
  • Neurology (AREA)
  • Orthopedic Medicine & Surgery (AREA)
  • Dermatology (AREA)
  • Obesity (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
DK06008807T 1999-08-30 2000-08-29 Sekvensspecifik DNA-rekombination i eukaryote celler DK1681355T3 (da)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE19941186A DE19941186A1 (de) 1999-08-30 1999-08-30 Sequenz-spezifische DNA-Rekombination in eukaryotischen Zellen
PCT/DE2000/002947 WO2001016345A2 (de) 1999-08-30 2000-08-29 Sequenz-spezifische dna-rekombination in eukaryotischen zellen

Publications (1)

Publication Number Publication Date
DK1681355T3 true DK1681355T3 (da) 2009-12-14

Family

ID=7920135

Family Applications (2)

Application Number Title Priority Date Filing Date
DK00965792T DK1214440T3 (da) 1999-08-30 2000-08-29 Sekvensspecifik DNA-rekombination i eukaryote celler
DK06008807T DK1681355T3 (da) 1999-08-30 2000-08-29 Sekvensspecifik DNA-rekombination i eukaryote celler

Family Applications Before (1)

Application Number Title Priority Date Filing Date
DK00965792T DK1214440T3 (da) 1999-08-30 2000-08-29 Sekvensspecifik DNA-rekombination i eukaryote celler

Country Status (19)

Country Link
US (2) US20030027337A1 (da)
EP (2) EP1681355B1 (da)
JP (1) JP2003520028A (da)
KR (1) KR100490188B1 (da)
CN (1) CN1182247C (da)
AT (2) ATE331802T1 (da)
AU (1) AU776297B2 (da)
CA (1) CA2390526C (da)
CY (2) CY1105288T1 (da)
CZ (1) CZ302620B6 (da)
DE (3) DE19941186A1 (da)
DK (2) DK1214440T3 (da)
ES (2) ES2267567T3 (da)
HK (1) HK1047129B (da)
IL (2) IL148152A0 (da)
MX (1) MX244498B (da)
PT (2) PT1681355E (da)
SK (1) SK286034B6 (da)
WO (1) WO2001016345A2 (da)

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US6964861B1 (en) * 1998-11-13 2005-11-15 Invitrogen Corporation Enhanced in vitro recombinational cloning of using ribosomal proteins
US6143557A (en) 1995-06-07 2000-11-07 Life Technologies, Inc. Recombination cloning using engineered recombination sites
US6720140B1 (en) 1995-06-07 2004-04-13 Invitrogen Corporation Recombinational cloning using engineered recombination sites
US7351578B2 (en) * 1999-12-10 2008-04-01 Invitrogen Corp. Use of multiple recombination sites with unique specificity in recombinational cloning
JP4303418B2 (ja) * 1997-10-24 2009-07-29 ライフ テクノロジーズ コーポレーション 組換え部位を有する核酸を使用する組換えクローニング
EP2119788A1 (en) * 1999-03-02 2009-11-18 Life Technologies Corporation Compositions and methods for use in recombinational cloning of nucleic acids
CN1757724B (zh) 1999-12-10 2014-06-11 茵维特罗根公司 具有独特特异性的多个重组位点在重组克隆中的用途
US7244560B2 (en) 2000-05-21 2007-07-17 Invitrogen Corporation Methods and compositions for synthesis of nucleic acid molecules using multiple recognition sites
US7198924B2 (en) * 2000-12-11 2007-04-03 Invitrogen Corporation Methods and compositions for synthesis of nucleic acid molecules using multiple recognition sites
CA2444195A1 (en) * 2001-04-19 2002-10-31 Invitrogen Corporation Compositions and methods for recombinational cloning of nucleic acid molecules
JP2004532636A (ja) * 2001-05-21 2004-10-28 インヴィトロジェン コーポレーション 核酸分子の単離に用いるための組成物および方法
US20050287647A9 (en) * 2001-05-30 2005-12-29 Carl Perez Plant artificial chromosomes, uses thereof and methods of preparing plant artificial chromosomes
CA2441937A1 (en) * 2001-05-30 2002-12-05 Chromos Molecular Systems, Inc. Chromosome-based platforms
US7351877B2 (en) 2002-03-29 2008-04-01 Syngenta Participations Ag Lambda integrase mediated recombination in plants
US20040219516A1 (en) * 2002-07-18 2004-11-04 Invitrogen Corporation Viral vectors containing recombination sites
TW200502390A (en) 2002-11-28 2005-01-16 Peter Droege Sequence specific DNA recombination in eukaryotic cells
US7491539B2 (en) 2002-12-05 2009-02-17 Boehringer Ingelheim Pharma Kg Sequence specific DNA recombination in eukaryotic cells
US20050095615A1 (en) * 2003-06-26 2005-05-05 Welch Peter J. Methods and compositions for detecting promoter activity and expressing fusion proteins
US20050069929A1 (en) 2003-08-08 2005-03-31 Invitrogen Corporation Methods and compositions for seamless cloning of nucleic acid molecules
US8304189B2 (en) * 2003-12-01 2012-11-06 Life Technologies Corporation Nucleic acid molecules containing recombination sites and methods of using the same
US7935862B2 (en) 2003-12-02 2011-05-03 Syngenta Participations Ag Targeted integration and stacking of DNA through homologous recombination
US8232456B2 (en) 2006-06-03 2012-07-31 Syngenta Participations Ag Corn event MIR162
WO2009068645A1 (en) * 2007-11-30 2009-06-04 Boehringer Ingelheim Pharma Gmbh & Co. Kg Novel recombination sequences
CN102604936B (zh) * 2011-01-21 2013-09-04 上海市儿童医院 羊基因组中被噬菌体*c31整合酶识别的dna及其应用
EP2739738B1 (en) 2011-08-03 2018-06-20 Ramot at Tel Aviv University, Ltd. Use of integrase for targeted gene expression
CN102533741B (zh) * 2011-12-09 2014-09-24 深圳华大基因研究院 猪假attp位点及其用途
US11078493B2 (en) 2016-06-21 2021-08-03 Nanyang Technological University Site-specific DNA recombination
JP7402503B2 (ja) 2017-06-14 2023-12-21 テクニスチェ ユニベルシタト ドレスデン デザイナーｄｎａ組換え酵素を利用したゲノムを遺伝的に改変するための方法及び手段
US20250354175A1 (en) * 2022-06-07 2025-11-20 Institut National De Recherche Pour L'agriculture, L'alimentation Et L'environnement Attp mv4-derived site-specific recombination and its use for integration of sequence of interest

Family Cites Families (14)

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US5464764A (en) * 1989-08-22 1995-11-07 University Of Utah Research Foundation Positive-negative selection methods and vectors
US7118911B1 (en) * 1990-03-05 2006-10-10 Genzyme Corporation DNA molecules stabilized for propagation in bacterial cells that encode cystic fibrosis transmembrane conductance regulator
US5227288A (en) * 1990-10-01 1993-07-13 Blattner Frederick R DNA sequencing vector with reversible insert
US5733743A (en) * 1992-03-24 1998-03-31 Cambridge Antibody Technology Limited Methods for producing members of specific binding pairs
US5861273A (en) * 1993-12-21 1999-01-19 Celtrix Phamraceuticals, Inc. Chromosomal expression of heterologous genes in bacterial cells
FR2731014B1 (fr) * 1995-02-23 1997-03-28 Rhone Poulenc Rorer Sa Molecules d'adn, preparation et utilisation en therapie genique
NZ500843A (en) * 1995-06-07 2002-03-28 Invitrogen Corp A method of DNA recombination which is selectable to select for cells containing a product and against cells only harbouring the insert donor
DE19530412A1 (de) * 1995-08-18 1997-02-20 Harald Von Prof Dr Melchner Selbst deletierende retrovirale Vektoren für die Gentherapie
FR2741892B1 (fr) * 1995-12-04 1998-02-13 Pasteur Merieux Serums Vacc Procede de preparation d'une banque multicombinatoire de vecteurs d'expression de genes d'anticorps, banque et systemes d'expression d'anticorps "coliclonaux" obtenus
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US5851808A (en) * 1997-02-28 1998-12-22 Baylor College Of Medicine Rapid subcloning using site-specific recombination
JP4303418B2 (ja) * 1997-10-24 2009-07-29 ライフ テクノロジーズ コーポレーション 組換え部位を有する核酸を使用する組換えクローニング
WO2000011155A1 (en) * 1998-08-19 2000-03-02 The Board Of Trustees Of The Leland Stanford Junior University Methods and compositions for genomic modification

Also Published As

Publication number Publication date
CY1105288T1 (el) 2010-03-03
DE50015772D1 (de) 2009-12-03
CA2390526A1 (en) 2001-03-08
CY1110590T1 (el) 2015-04-29
EP1681355A1 (de) 2006-07-19
DE50013093D1 (de) 2006-08-10
CN1182247C (zh) 2004-12-29
CZ302620B6 (cs) 2011-08-03
DK1214440T3 (da) 2006-10-30
EP1681355B1 (de) 2009-10-21
KR100490188B1 (ko) 2005-05-17
DE19941186A1 (de) 2001-03-01
WO2001016345A3 (de) 2001-12-06
CZ2002756A3 (cs) 2002-06-12
ATE331802T1 (de) 2006-07-15
KR20020057953A (ko) 2002-07-12
US20130133092A1 (en) 2013-05-23
AU776297B2 (en) 2004-09-02
CN1387576A (zh) 2002-12-25
ATE446373T1 (de) 2009-11-15
US20030027337A1 (en) 2003-02-06
PT1214440E (pt) 2006-11-30
MX244498B (es) 2007-03-28
IL148152A (en) 2007-10-31
JP2003520028A (ja) 2003-07-02
AU7642900A (en) 2001-03-26
ES2334577T3 (es) 2010-03-12
EP1214440B1 (de) 2006-06-28
ES2267567T3 (es) 2007-03-16
MXPA02002036A (es) 2003-08-20
SK3042002A3 (en) 2002-07-02
EP1214440A2 (de) 2002-06-19
CA2390526C (en) 2010-10-19
SK286034B6 (sk) 2008-01-07
IL148152A0 (en) 2002-09-12
WO2001016345A2 (de) 2001-03-08
HK1047129A1 (en) 2003-02-07
HK1047129B (zh) 2006-10-20
PT1681355E (pt) 2010-01-27

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