DK1749096T3 - Fremgangsmåde til fremstilling af egnede DNA-konstrukter til specifik inhibering af genekspression ved RNA-interferens - Google Patents

Fremgangsmåde til fremstilling af egnede DNA-konstrukter til specifik inhibering af genekspression ved RNA-interferens

Info

Publication number
DK1749096T3
DK1749096T3 DK04816287.9T DK04816287T DK1749096T3 DK 1749096 T3 DK1749096 T3 DK 1749096T3 DK 04816287 T DK04816287 T DK 04816287T DK 1749096 T3 DK1749096 T3 DK 1749096T3
Authority
DK
Denmark
Prior art keywords
gene expression
rna interference
dna constructs
specific inhibition
suitable dna
Prior art date
Application number
DK04816287.9T
Other languages
English (en)
Inventor
Matthias Schroff
Original Assignee
Mologen Ag
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Mologen Ag filed Critical Mologen Ag
Application granted granted Critical
Publication of DK1749096T3 publication Critical patent/DK1749096T3/da

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/111—General methods applicable to biologically active non-coding nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/11—Antisense
    • C12N2310/111—Antisense spanning the whole gene, or a large part of it
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/14—Type of nucleic acid interfering nucleic acids [NA]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2320/00—Applications; Uses
    • C12N2320/10—Applications; Uses in screening processes

Landscapes

  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Molecular Biology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
DK04816287.9T 2004-05-28 2004-12-28 Fremgangsmåde til fremstilling af egnede DNA-konstrukter til specifik inhibering af genekspression ved RNA-interferens DK1749096T3 (da)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE102004026734 2004-05-28
PCT/DE2004/002838 WO2005116223A1 (de) 2004-05-28 2004-12-28 Herstellungsverfahren geeigneter dna-konstrukte zur spezifischen hemmung der genexpression durch rna-interferenz

Publications (1)

Publication Number Publication Date
DK1749096T3 true DK1749096T3 (da) 2013-10-28

Family

ID=34960317

Family Applications (1)

Application Number Title Priority Date Filing Date
DK04816287.9T DK1749096T3 (da) 2004-05-28 2004-12-28 Fremgangsmåde til fremstilling af egnede DNA-konstrukter til specifik inhibering af genekspression ved RNA-interferens

Country Status (4)

Country Link
US (1) US20090004703A1 (da)
EP (1) EP1749096B1 (da)
DK (1) DK1749096T3 (da)
WO (1) WO2005116223A1 (da)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7627817B2 (en) 2003-02-21 2009-12-01 Motionpoint Corporation Analyzing web site for translation
CN1918293A (zh) * 2004-02-20 2007-02-21 莫洛根股份公司 用于对人及高等动物进行治疗性和预防性免疫刺激的取代的非编码核酸分子
WO2007059760A1 (de) * 2005-11-25 2007-05-31 Mologen Ag Dna-konstrukte zur spezifischen hemmung der genexpression durch rna-interferenz
EP2680160B1 (en) 2010-07-13 2019-12-11 Motionpoint Corporation Methods of Uniform Resource Locator (URL) translation
WO2016058171A1 (en) * 2014-10-17 2016-04-21 Shanghai Sidansai Biotechnology Co.,Ltd Method for directional cloning
LU92821B1 (en) 2015-09-09 2017-03-20 Mologen Ag Combination comprising immunostimulatory oligonucleotides
GB2542425A (en) 2015-09-21 2017-03-22 Mologen Ag Means for the treatment of HIV

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2675803B1 (fr) * 1991-04-25 1996-09-06 Genset Sa Oligonucleotides fermes, antisens et sens et leurs applications.
DE19648625A1 (de) * 1996-11-13 1998-05-14 Soft Gene Gmbh Mikroprojektil für das Einbringen von Substanzen in Zellen durch ballistischen Transfer
DE19826758C1 (de) * 1998-06-15 1999-10-21 Soft Gene Gmbh Darstellung von linearen kovalent geschlossenen DNA-Molekülen als Expressionskonstrukte
AU2002343792A1 (en) * 2001-11-28 2003-06-10 Center For Advanced Science And Technology Incubation, Ltd. siRNA EXPRESSION SYSTEM AND PROCESS FOR PRODUCING FUNCTIONAL GENE-KNOCKDOWN CELLS AND THE LIKE USING THE SAME
GB0130955D0 (en) * 2001-12-24 2002-02-13 Cancer Res Ventures Expression system
US20060257380A1 (en) * 2002-09-19 2006-11-16 Inst.Nat. De La Sante Et De La Recherche MED Use of sirnas for gene silencing in antigen presenting cells
CA2523785A1 (en) * 2003-05-09 2004-11-25 University Of Pittsburgh Of The Commonwealth System Of Higher Education Small interfering rna libraries and methods of synthesis and use
US20050064489A1 (en) * 2003-09-24 2005-03-24 Zhang Fang Liang Engineered U6 and H1 promoters
US20050233994A1 (en) * 2004-04-16 2005-10-20 Ajamete Kaykas Methods and vectors for expressing siRNA

Also Published As

Publication number Publication date
EP1749096A1 (de) 2007-02-07
WO2005116223A1 (de) 2005-12-08
EP1749096B1 (de) 2013-07-17
US20090004703A1 (en) 2009-01-01

Similar Documents

Publication Publication Date Title
IL190114A0 (en) Inhibition of viral gene expression using small interfering rna
DK1658365T3 (da) Fremgangsmåde til genkloning af produktionsceller
IL186689A0 (en) Methods of enhancing isolation of rna from biological samples
EP2294225B8 (en) Method for direct amplification from crude nucleic acid samples
EP2606134A4 (en) INTERFERENT RNA-MEDIATED INHIBITION OF GENE EXPRESSION OF HEPATITIS B (HBV) VIRUS USING SMALL INTERFERING NUCLEIC ACIDS (PANI)
GB0507019D0 (en) RNA interference mediated treatment of polyglutamine (polyq) repeat expansion diseases using short interfering nucleic acid (sina)
ZA200706142B (en) Methods for simplifying microbial nucleic acids by chemical modification of cytosines
IL214301A0 (en) Method for quantification of small rna species
AU2003297474A8 (en) Methods of inhibiting gene expression by rna interference
DK1745136T3 (da) Fremgangsmåde til enzymatisk fremstilling af (S)-3-cyano-5-methylhexansyre
ATE439438T1 (de) Durch rna-interferenz vermittelte hemmung der genexpression unter verwendung von chemisch modifizierter sina (short interfering nucleic acid)
EP1781800A4 (en) PROCESS FOR PLASMID DNA FERMENTATION
EP2474619A4 (en) A SPECIFIC METHOD FOR THE PRODUCTION OF LINKED DNA FRAGMENTS WITH TARGETED SEQUENCES
AU2003213054A8 (en) Rna interference mediated inhibition of polycomb group protein ezh2 gene expression using short interfering nucleic acid (sina)
EP1476459A4 (en) INHIBITION OF RNA INTERFERENCE-INDUCED CHROMOSOME TRANSLOCATION GENE EXPRESSION USING SHORT INTERFERING NUCLEIC ACID (SINA)
DK1951870T3 (da) DNA-konstrukter til specifik inhibering af genekspression ved RNA-interferens
IL193411A0 (en) Methods of extracting nucleic acids
EP2167524A4 (en) METHOD FOR DIRECTLY AMPLIFYING NUCLEIC ACIDS FROM NON-CLEANED SAMPLES
DK1749096T3 (da) Fremgangsmåde til fremstilling af egnede DNA-konstrukter til specifik inhibering af genekspression ved RNA-interferens
GB0609420D0 (en) Method of gene transfer
AU2003276765A1 (en) Method for amplifying unknown dna sequence adjacent to known sequence
EP2010670A4 (en) METHOD FOR THE AMPLIFICATION OF AN UNKNOWN DNA SEQUENCE OUTSTANDING A KNOWN SEQUENCE
IL192182A0 (en) RNA INTERFERENCE MEDIATED INHIBITION OF HEPATITIS C VIRUS (HCV) GENE EXPRESSION USING SHORT INTERFERING NUCLEIC ACID (siNA)
AU2003234030A8 (en) Method of selecting targets for gene silencing by rna interference
DK1926821T3 (da) Fremgangsmåde til enantioselektiv enzymatisk reduktion af ketoforbindelser