DK176238B1 - In vitro isolering og opretholdelse af embryone stængelceller, (ES)-celler, samt fremgangsmåde til fremstilling af et non-humant kimært dyr - Google Patents

In vitro isolering og opretholdelse af embryone stængelceller, (ES)-celler, samt fremgangsmåde til fremstilling af et non-humant kimært dyr Download PDF

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Publication number
DK176238B1
DK176238B1 DK199100170A DK17091A DK176238B1 DK 176238 B1 DK176238 B1 DK 176238B1 DK 199100170 A DK199100170 A DK 199100170A DK 17091 A DK17091 A DK 17091A DK 176238 B1 DK176238 B1 DK 176238B1
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Prior art keywords
cells
lif
culture medium
animal
embryos
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DK199100170A
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Danish (da)
English (en)
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DK17091D0 (da
DK17091A (da
Inventor
Robert Lindsay Williams
Nicholas Martin Gough
Douglas James Hilton
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Amrad Corp Ltd A Corp Of The S
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Publication of DK17091A publication Critical patent/DK17091A/da
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01KANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
    • A01K67/027New or modified breeds of vertebrates
    • A01K67/0275Genetically modified vertebrates, e.g. transgenic
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06Animal cells or tissues; Human cells or tissues
    • C12N5/0602Vertebrate cells
    • C12N5/0603Embryonic cells ; Embryoid bodies
    • C12N5/0606Pluripotent embryonic cells, e.g. embryonic stem cells [ES]
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01KANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00Genetically modified animals
    • A01K2217/05Animals comprising random inserted nucleic acids (transgenic)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00Active agents used in cell culture processes, e.g. differentation
    • C12N2501/20Cytokines; Chemokines
    • C12N2501/23Interleukins [IL]
    • C12N2501/235Leukemia inhibitory factor [LIF]
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2510/00Genetically modified cells

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Environmental Sciences (AREA)
  • Developmental Biology & Embryology (AREA)
  • Gynecology & Obstetrics (AREA)
  • Reproductive Health (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Animal Behavior & Ethology (AREA)
  • Cell Biology (AREA)
  • Microbiology (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Animal Husbandry (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Veterinary Medicine (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
DK199100170A 1988-08-04 1991-01-31 In vitro isolering og opretholdelse af embryone stængelceller, (ES)-celler, samt fremgangsmåde til fremstilling af et non-humant kimært dyr DK176238B1 (da)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
AUPI964488 1988-08-04
AUPI964488 1988-08-04
AU8900330 1989-08-03
PCT/AU1989/000330 WO1990001541A1 (en) 1988-08-04 1989-08-03 In vitro propagation of embryonic stem cells

Publications (3)

Publication Number Publication Date
DK17091D0 DK17091D0 (da) 1991-01-31
DK17091A DK17091A (da) 1991-04-04
DK176238B1 true DK176238B1 (da) 2007-04-10

Family

ID=3773256

Family Applications (1)

Application Number Title Priority Date Filing Date
DK199100170A DK176238B1 (da) 1988-08-04 1991-01-31 In vitro isolering og opretholdelse af embryone stængelceller, (ES)-celler, samt fremgangsmåde til fremstilling af et non-humant kimært dyr

Country Status (10)

Country Link
US (1) US5166065A (de)
EP (1) EP0380646B1 (de)
JP (1) JP2740320B2 (de)
AT (1) ATE175994T1 (de)
AU (1) AU623922B2 (de)
CA (1) CA1341469C (de)
DE (1) DE68928914T2 (de)
DK (1) DK176238B1 (de)
NO (1) NO322243B1 (de)
WO (1) WO1990001541A1 (de)

Families Citing this family (99)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20030032178A1 (en) * 1988-08-04 2003-02-13 Williams Robert Lindsay In vitro propagation of embryonic stem cells
AU622515B2 (en) * 1989-01-10 1992-04-09 Amrad Operations Pty. Limited Leukaemia inhibitory factor from livestock species and use thereof to enhance implantation and development of embryonic cells
DE69028514T2 (de) * 1989-01-10 1997-02-20 Amrad Corp Ltd Leukämiehemmender faktor aus vieharten und seine verwendung zur verbesserung der implantation und der entwicklung von embryonalen zellen
US5705732A (en) * 1989-06-12 1998-01-06 Oklahoma Medical Research Foundation Universal donor cells
JP3040815B2 (ja) * 1989-11-24 2000-05-15 モナッシュ・ユニバーシティ 随伴細胞に及ぼす白血病抑制因子の増殖作用
JP3049091B2 (ja) * 1990-03-20 2000-06-05 アムラド・コーポレイション・リミテッド ニューロン増殖及び維持を調節する方法
WO1992001040A1 (en) * 1990-07-09 1992-01-23 Amrad Corporation Limited Enhanced implantation, development and maintenance of embryos using leukaemia inhibitory factor
ES2237747T3 (es) * 1991-11-14 2005-08-01 Prionics Ag Animales transgenicos no humanos que carecen de proteinas prionicas.
US5453357A (en) * 1992-10-08 1995-09-26 Vanderbilt University Pluripotential embryonic stem cells and methods of making same
US7153684B1 (en) 1992-10-08 2006-12-26 Vanderbilt University Pluripotential embryonic stem cells and methods of making same
US5690926A (en) * 1992-10-08 1997-11-25 Vanderbilt University Pluripotential embryonic cells and methods of making same
GB9308271D0 (en) * 1993-04-21 1993-06-02 Univ Edinburgh Method of isolating and/or enriching and/or selectively propagating pluripotential animal cells and animals for use in said method
US5523226A (en) * 1993-05-14 1996-06-04 Biotechnology Research And Development Corp. Transgenic swine compositions and methods
WO1995006716A1 (en) * 1993-08-30 1995-03-09 Northwestern University Rat pluripotent embryonic stem cells and method of obtaining and using same
US6686198B1 (en) * 1993-10-14 2004-02-03 President And Fellows Of Harvard College Method of inducing and maintaining neuronal cells
DE69434934D1 (de) * 1993-10-14 2007-04-12 Harvard College Verfahren zur induzierung und zur erhaltung neuronalen zellen
US6432711B1 (en) * 1993-11-03 2002-08-13 Diacrin, Inc. Embryonic stem cells capable of differentiating into desired cell lines
GB9401380D0 (en) * 1994-01-25 1994-03-23 Pharmaceutical Proteins Ltd Embryonic cell isolation
FR2726003B1 (fr) * 1994-10-21 2002-10-18 Agronomique Inst Nat Rech Milieu de culture de cellules embryonnaires totipotentes aviaires, procede de culture de ces cellules, et cellules embryonnaires totipotentes aviaires
US5874301A (en) * 1994-11-21 1999-02-23 National Jewish Center For Immunology And Respiratory Medicine Embryonic cell populations and methods to isolate such populations
US5843780A (en) 1995-01-20 1998-12-01 Wisconsin Alumni Research Foundation Primate embryonic stem cells
US5728581A (en) * 1995-06-07 1998-03-17 Systemix, Inc. Method of expanding hematopoietic stem cells, reagents and bioreactors for use therein
CA2242666A1 (en) 1996-01-09 1997-07-17 The Regents Of The University Of California Ungulate embryonic stem-like cells, methods of making and using the cells to produce transgenic ungulates
AU4353696A (en) 1996-01-09 1997-08-01 Regents Of The University Of California, The Ungulate embryonic stem-like cells, making and using the cells to produce transgenic ungulate
US6247995B1 (en) 1996-02-06 2001-06-19 Bruce Bryan Bioluminescent novelty items
US5876995A (en) 1996-02-06 1999-03-02 Bryan; Bruce Bioluminescent novelty items
AU2192997A (en) * 1996-02-28 1997-09-16 Vanderbilt University Compositions and methods of making embryonic stem cells
CN1217746A (zh) * 1996-04-29 1999-05-26 勒芬研究与发展公司 多能性兔胚胎干(es)细胞系及其在嵌合兔的产生中的应用
US7544511B2 (en) * 1996-09-25 2009-06-09 Neuralstem Biopharmaceuticals Ltd. Stable neural stem cell line methods
AU3392697A (en) * 1996-06-14 1998-01-07 Regents Of The University Of California, The (in vitro) derivation and culture of primate pluripotent stem cells and therapeutic uses thereof
US6416960B1 (en) 1996-08-08 2002-07-09 Prolume, Ltd. Detection and visualization of neoplastic tissues and other tissues
EP1015872B1 (de) 1996-12-12 2005-03-02 Prolume, Ltd. Vorrichtung und verfahren zum nachweis und identifizieren von infektiösen wirkstoffen
US6011197A (en) 1997-03-06 2000-01-04 Infigen, Inc. Method of cloning bovines using reprogrammed non-embryonic bovine cells
NZ337495A (en) * 1997-03-06 2001-06-29 Infigen Inc Bovine primordial germ cells and their use in cloning
US6090622A (en) * 1997-03-31 2000-07-18 The Johns Hopkins School Of Medicine Human embryonic pluripotent germ cells
US6331406B1 (en) 1997-03-31 2001-12-18 The John Hopkins University School Of Medicine Human enbryonic germ cell and methods of use
AU729377B2 (en) 1997-10-23 2001-02-01 Asterias Biotherapeutics, Inc. Methods and materials for the growth of primate-derived primordial stem cells in feeder-free culture
US6027937A (en) * 1998-08-18 2000-02-22 Harper; William Method for propagation of insecta germ cells
US6767737B1 (en) * 1998-08-31 2004-07-27 New York University Stem cells bearing an FGF receptor on the cell surface
US6667176B1 (en) * 2000-01-11 2003-12-23 Geron Corporation cDNA libraries reflecting gene expression during growth and differentiation of human pluripotent stem cells
US7410798B2 (en) * 2001-01-10 2008-08-12 Geron Corporation Culture system for rapid expansion of human embryonic stem cells
US20020168766A1 (en) * 2000-01-11 2002-11-14 Gold Joseph D. Genetically altered human pluripotent stem cells
ATE408667T1 (de) * 1999-07-20 2008-10-15 Univ Southern California Identifizierung pluripotenter prämesenchymaler, prähämotopoietischer vorläuferzellen
US6333192B1 (en) * 1999-08-09 2001-12-25 North Carolina State University Method of producing an undifferentiated avian cell culture using avian primordial germ cells
US7455983B2 (en) * 2000-01-11 2008-11-25 Geron Corporation Medium for growing human embryonic stem cells
US6828145B2 (en) 2000-05-10 2004-12-07 Cedars-Sinai Medical Center Method for the isolation of stem cells by immuno-labeling with HLA/MHC gene product marker
WO2001091548A2 (en) * 2000-06-01 2001-12-06 Pharmacia & Upjohn Company Mice heterozygous for wfs1 gene as mouse models for depression
KR100865664B1 (ko) * 2000-06-14 2008-10-29 비스타겐 인코포레이티드 간 줄기 세포를 사용하는 독성 타이핑
EA013564B1 (ru) * 2000-08-03 2010-06-30 Терапеутик Хьюман Поликлоналз Инк. Гуманизированный иммуноглобулин и содержащая его фармацевтическая композиция
ATE466089T1 (de) 2000-12-22 2010-05-15 Agronomique Inst Nat Rech Positionsunabhängige und gewebespezifische expression eines transgens in der milch eines transgen-tieres
CA2435826A1 (en) * 2001-01-24 2002-08-01 The Government Of The United States Of America Differentiation of stem cells to pancreatic endocrine cells
US20030211605A1 (en) * 2001-05-01 2003-11-13 Lee Sang-Hun Derivation of midbrain dopaminergic neurons from embryonic stem cells
US7129034B2 (en) * 2001-10-25 2006-10-31 Cedars-Sinai Medical Center Differentiation of whole bone marrow
US6759244B2 (en) 2001-11-08 2004-07-06 Art Institute Of New York And New Jersey, Inc. Composite blastocysts (CBs) from aggregates of dissociated cells of non-viable pre-embryos
GB0220145D0 (en) * 2002-08-30 2002-10-09 Thromb X Nv Novel compositions for the in vitro derivation and culture of embryonic stem (ES) cell lines with germline transmission capability
WO2003054169A1 (en) * 2001-12-21 2003-07-03 Thromb-X Nv Compositions for the in vitro derivation and culture of embryonic stem (es) cell lines with germline transmission capability
JP4408371B2 (ja) * 2001-12-21 2010-02-03 トロンボジェニクス・ナムローゼ・フエンノートシャップ 生殖細胞系伝達能力を有する胚性幹(es)細胞のインビトロの誘導および培養のための組成物
US7145057B2 (en) 2002-02-01 2006-12-05 Origen Therapeutics, Inc. Chimeric bird from embryonic stem cells
FR2836924B1 (fr) 2002-03-08 2005-01-14 Vivalis Lignees de cellules aviaires utiles pour la production de substances d'interet
US20030207448A1 (en) * 2002-05-06 2003-11-06 Revera Gregory Henry Methodologies for the creation of pluripotent or multipotent human stem cells without creating or destroying a human embryo
CA2504179A1 (en) * 2002-10-31 2004-05-13 Riken Composition for culturing multipotent stem cells and utilization of the same
US8293488B2 (en) 2002-12-09 2012-10-23 Neuralstem, Inc. Method for screening neurogenic agents
AU2003293409A1 (en) * 2002-12-09 2004-06-30 Karl K. Johe Method for discovering neurogenic agents
ES2705683T3 (es) * 2002-12-16 2019-03-26 Technion Res & Dev Foundation Medio de cultivo de células madre pluripotentes
US20040191839A1 (en) * 2003-03-28 2004-09-30 National Institute Of Agrobiological Sciences Japan Methods for sorting undifferentiated cells and uses thereof
IL155783A (en) 2003-05-05 2010-11-30 Technion Res & Dev Foundation Multicellular systems of pluripotent human embryonic stem cells and cancer cells and uses thereof
WO2006040615A2 (en) * 2003-06-11 2006-04-20 Jan Remmereit Differenciation of stem cells for therapeutic use
CA2569978C (en) * 2004-06-09 2017-06-06 The University Court Of The University Of Edinburgh Neural stem cells
US20050277124A1 (en) * 2004-06-10 2005-12-15 White Steven M Cardiac conduction system cells and uses thereof
WO2005123900A1 (en) * 2004-06-10 2005-12-29 Merial Limited Medium and methods for culturing of avian primordial germ cells
WO2006023422A2 (en) * 2004-08-16 2006-03-02 Kouns George S Stem-like cells
CN101084303A (zh) * 2004-11-17 2007-12-05 神经干公司 用于治疗神经变性症状的人类神经细胞的移植
CN1298843C (zh) * 2005-02-07 2007-02-07 十堰市太和医院 不依赖饲养细胞的人胚干细胞培养基
KR20160116024A (ko) * 2005-06-22 2016-10-06 아스테리아스 바이오세라퓨틱스, 인크. 인간 배아 줄기 세포의 현탁 배양
EP2422802A3 (de) 2006-03-07 2013-03-06 Geeta Shroff Zusammentsetzungen enhaltend menschliche embryonale Stammzellen und ihre Derivate, Verwendung davon und Verfahren zur ihrer Vorbereitung
AU2007285483B2 (en) 2006-05-11 2011-12-22 Regenics As Administration of cells and cellular extracts for rejuvenation
US20080003676A1 (en) * 2006-06-26 2008-01-03 Millipore Corporation Growth of embryonic stem cells
EP2500417A1 (de) 2006-08-09 2012-09-19 Vivalis Verfahren zur Herstellung von transgenen Vögeln unter Verwendung von embryonalen Stammzellen
ATE536374T1 (de) 2006-09-01 2011-12-15 Therapeutic Human Polyclonals Inc Erhöhte expression von humanem oder humanisiertem immunglobulin bei nicht-humanen transgenen tieren
US8685720B2 (en) 2006-11-03 2014-04-01 The Trustees Of Princeton University Engineered cellular pathways for programmed autoregulation of differentiation
US20080206864A1 (en) * 2006-11-22 2008-08-28 Derrick Rancourt Methods and Materials for Culturing Murine Embryonic Stem Cells
US20080267874A1 (en) * 2007-03-28 2008-10-30 The Buck Institute For Age Research Targeted Neuronal And Glial Human Embryonic Stem Cell Line
EP1985305A1 (de) 2007-04-24 2008-10-29 Vivalis Aus Entenembryonen gewonnene Stammzellkulturen zur Herstellung von Impfstoffen gegen Viren
CA2723848C (en) 2008-05-09 2013-01-08 Regenics As Method of making salmon egg cytoplasmic extracts and use for increasing collagen production in skin
US20100158878A1 (en) 2008-12-23 2010-06-24 Alexandra Capela Target populations of oligodendrocyte precursor cells and methods of making and using same
US8642334B2 (en) 2009-02-17 2014-02-04 Memorial Sloan Kettering Cancer Center Methods of neural conversion of human embryonic stem cells
CN101962630B (zh) * 2009-07-23 2013-04-10 北京大学 诱导人胚胎干细胞或人诱导形成的多潜能干细胞向肝细胞分化的方法
AU2011249478B2 (en) 2010-05-06 2014-12-04 Regenics As Use of cellular extracts for skin rejuvenation
CN103237553A (zh) 2010-07-28 2013-08-07 神经干细胞公司 治疗和/或逆转神经退行性疾病和/或病症的方法
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EP3498824A1 (de) 2013-04-26 2019-06-19 Memorial Sloan-Kettering Cancer Center Durch gezielte differenzierung pluripotenter und multipotenter zellen hergestellte kortikale interneuronen und andere neuronale zellen
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CN109689089A (zh) 2016-05-25 2019-04-26 国家医疗保健研究所 治疗癌症的方法和组合物
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WO2020030634A1 (en) 2018-08-06 2020-02-13 INSERM (Institut National de la Santé et de la Recherche Médicale) Methods and compositions for treating cancers
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Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
PT87133B (pt) * 1987-04-02 1992-07-31 Amrad Corp Ltd Metodo de purificacao do factor inibidor da leucemia (lif) e de composicoes farmaceuticas contendo polipeptidos com actividade do lif

Also Published As

Publication number Publication date
DK17091D0 (da) 1991-01-31
JPH03503241A (ja) 1991-07-25
DE68928914D1 (de) 1999-03-04
JP2740320B2 (ja) 1998-04-15
HK1003208A1 (en) 1998-10-16
NO322243B1 (no) 2006-09-04
AU623922B2 (en) 1992-05-28
CA1341469C (en) 2004-12-28
NO910385L (no) 1991-04-04
EP0380646A4 (en) 1991-01-23
DK17091A (da) 1991-04-04
US5166065A (en) 1992-11-24
AU4059089A (en) 1990-03-05
ATE175994T1 (de) 1999-02-15
DE68928914T2 (de) 1999-09-09
WO1990001541A1 (en) 1990-02-22
NO910385D0 (no) 1991-02-01
EP0380646B1 (de) 1999-01-20
EP0380646A1 (de) 1990-08-08

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