DK176238B1 - In vitro isolering og opretholdelse af embryone stængelceller, (ES)-celler, samt fremgangsmåde til fremstilling af et non-humant kimært dyr - Google Patents
In vitro isolering og opretholdelse af embryone stængelceller, (ES)-celler, samt fremgangsmåde til fremstilling af et non-humant kimært dyr Download PDFInfo
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- DK176238B1 DK176238B1 DK199100170A DK17091A DK176238B1 DK 176238 B1 DK176238 B1 DK 176238B1 DK 199100170 A DK199100170 A DK 199100170A DK 17091 A DK17091 A DK 17091A DK 176238 B1 DK176238 B1 DK 176238B1
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Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/027—New or modified breeds of vertebrates
- A01K67/0275—Genetically modified vertebrates, e.g. transgenic
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0603—Embryonic cells ; Embryoid bodies
- C12N5/0606—Pluripotent embryonic cells, e.g. embryonic stem cells [ES]
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K2217/00—Genetically modified animals
- A01K2217/05—Animals comprising random inserted nucleic acids (transgenic)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2501/00—Active agents used in cell culture processes, e.g. differentation
- C12N2501/20—Cytokines; Chemokines
- C12N2501/23—Interleukins [IL]
- C12N2501/235—Leukemia inhibitory factor [LIF]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2510/00—Genetically modified cells
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Zoology (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Environmental Sciences (AREA)
- Developmental Biology & Embryology (AREA)
- Gynecology & Obstetrics (AREA)
- Reproductive Health (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Animal Behavior & Ethology (AREA)
- Cell Biology (AREA)
- Microbiology (AREA)
- Biodiversity & Conservation Biology (AREA)
- Animal Husbandry (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- Veterinary Medicine (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Applications Claiming Priority (4)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| AUPI964488 | 1988-08-04 | ||
| AUPI964488 | 1988-08-04 | ||
| AU8900330 | 1989-08-03 | ||
| PCT/AU1989/000330 WO1990001541A1 (en) | 1988-08-04 | 1989-08-03 | In vitro propagation of embryonic stem cells |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| DK17091D0 DK17091D0 (da) | 1991-01-31 |
| DK17091A DK17091A (da) | 1991-04-04 |
| DK176238B1 true DK176238B1 (da) | 2007-04-10 |
Family
ID=3773256
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DK199100170A DK176238B1 (da) | 1988-08-04 | 1991-01-31 | In vitro isolering og opretholdelse af embryone stængelceller, (ES)-celler, samt fremgangsmåde til fremstilling af et non-humant kimært dyr |
Country Status (10)
| Country | Link |
|---|---|
| US (1) | US5166065A (de) |
| EP (1) | EP0380646B1 (de) |
| JP (1) | JP2740320B2 (de) |
| AT (1) | ATE175994T1 (de) |
| AU (1) | AU623922B2 (de) |
| CA (1) | CA1341469C (de) |
| DE (1) | DE68928914T2 (de) |
| DK (1) | DK176238B1 (de) |
| NO (1) | NO322243B1 (de) |
| WO (1) | WO1990001541A1 (de) |
Families Citing this family (99)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20030032178A1 (en) * | 1988-08-04 | 2003-02-13 | Williams Robert Lindsay | In vitro propagation of embryonic stem cells |
| AU622515B2 (en) * | 1989-01-10 | 1992-04-09 | Amrad Operations Pty. Limited | Leukaemia inhibitory factor from livestock species and use thereof to enhance implantation and development of embryonic cells |
| DE69028514T2 (de) * | 1989-01-10 | 1997-02-20 | Amrad Corp Ltd | Leukämiehemmender faktor aus vieharten und seine verwendung zur verbesserung der implantation und der entwicklung von embryonalen zellen |
| US5705732A (en) * | 1989-06-12 | 1998-01-06 | Oklahoma Medical Research Foundation | Universal donor cells |
| JP3040815B2 (ja) * | 1989-11-24 | 2000-05-15 | モナッシュ・ユニバーシティ | 随伴細胞に及ぼす白血病抑制因子の増殖作用 |
| JP3049091B2 (ja) * | 1990-03-20 | 2000-06-05 | アムラド・コーポレイション・リミテッド | ニューロン増殖及び維持を調節する方法 |
| WO1992001040A1 (en) * | 1990-07-09 | 1992-01-23 | Amrad Corporation Limited | Enhanced implantation, development and maintenance of embryos using leukaemia inhibitory factor |
| ES2237747T3 (es) * | 1991-11-14 | 2005-08-01 | Prionics Ag | Animales transgenicos no humanos que carecen de proteinas prionicas. |
| US5453357A (en) * | 1992-10-08 | 1995-09-26 | Vanderbilt University | Pluripotential embryonic stem cells and methods of making same |
| US7153684B1 (en) | 1992-10-08 | 2006-12-26 | Vanderbilt University | Pluripotential embryonic stem cells and methods of making same |
| US5690926A (en) * | 1992-10-08 | 1997-11-25 | Vanderbilt University | Pluripotential embryonic cells and methods of making same |
| GB9308271D0 (en) * | 1993-04-21 | 1993-06-02 | Univ Edinburgh | Method of isolating and/or enriching and/or selectively propagating pluripotential animal cells and animals for use in said method |
| US5523226A (en) * | 1993-05-14 | 1996-06-04 | Biotechnology Research And Development Corp. | Transgenic swine compositions and methods |
| WO1995006716A1 (en) * | 1993-08-30 | 1995-03-09 | Northwestern University | Rat pluripotent embryonic stem cells and method of obtaining and using same |
| US6686198B1 (en) * | 1993-10-14 | 2004-02-03 | President And Fellows Of Harvard College | Method of inducing and maintaining neuronal cells |
| DE69434934D1 (de) * | 1993-10-14 | 2007-04-12 | Harvard College | Verfahren zur induzierung und zur erhaltung neuronalen zellen |
| US6432711B1 (en) * | 1993-11-03 | 2002-08-13 | Diacrin, Inc. | Embryonic stem cells capable of differentiating into desired cell lines |
| GB9401380D0 (en) * | 1994-01-25 | 1994-03-23 | Pharmaceutical Proteins Ltd | Embryonic cell isolation |
| FR2726003B1 (fr) * | 1994-10-21 | 2002-10-18 | Agronomique Inst Nat Rech | Milieu de culture de cellules embryonnaires totipotentes aviaires, procede de culture de ces cellules, et cellules embryonnaires totipotentes aviaires |
| US5874301A (en) * | 1994-11-21 | 1999-02-23 | National Jewish Center For Immunology And Respiratory Medicine | Embryonic cell populations and methods to isolate such populations |
| US5843780A (en) | 1995-01-20 | 1998-12-01 | Wisconsin Alumni Research Foundation | Primate embryonic stem cells |
| US5728581A (en) * | 1995-06-07 | 1998-03-17 | Systemix, Inc. | Method of expanding hematopoietic stem cells, reagents and bioreactors for use therein |
| CA2242666A1 (en) | 1996-01-09 | 1997-07-17 | The Regents Of The University Of California | Ungulate embryonic stem-like cells, methods of making and using the cells to produce transgenic ungulates |
| AU4353696A (en) | 1996-01-09 | 1997-08-01 | Regents Of The University Of California, The | Ungulate embryonic stem-like cells, making and using the cells to produce transgenic ungulate |
| US6247995B1 (en) | 1996-02-06 | 2001-06-19 | Bruce Bryan | Bioluminescent novelty items |
| US5876995A (en) | 1996-02-06 | 1999-03-02 | Bryan; Bruce | Bioluminescent novelty items |
| AU2192997A (en) * | 1996-02-28 | 1997-09-16 | Vanderbilt University | Compositions and methods of making embryonic stem cells |
| CN1217746A (zh) * | 1996-04-29 | 1999-05-26 | 勒芬研究与发展公司 | 多能性兔胚胎干(es)细胞系及其在嵌合兔的产生中的应用 |
| US7544511B2 (en) * | 1996-09-25 | 2009-06-09 | Neuralstem Biopharmaceuticals Ltd. | Stable neural stem cell line methods |
| AU3392697A (en) * | 1996-06-14 | 1998-01-07 | Regents Of The University Of California, The | (in vitro) derivation and culture of primate pluripotent stem cells and therapeutic uses thereof |
| US6416960B1 (en) | 1996-08-08 | 2002-07-09 | Prolume, Ltd. | Detection and visualization of neoplastic tissues and other tissues |
| EP1015872B1 (de) | 1996-12-12 | 2005-03-02 | Prolume, Ltd. | Vorrichtung und verfahren zum nachweis und identifizieren von infektiösen wirkstoffen |
| US6011197A (en) | 1997-03-06 | 2000-01-04 | Infigen, Inc. | Method of cloning bovines using reprogrammed non-embryonic bovine cells |
| NZ337495A (en) * | 1997-03-06 | 2001-06-29 | Infigen Inc | Bovine primordial germ cells and their use in cloning |
| US6090622A (en) * | 1997-03-31 | 2000-07-18 | The Johns Hopkins School Of Medicine | Human embryonic pluripotent germ cells |
| US6331406B1 (en) | 1997-03-31 | 2001-12-18 | The John Hopkins University School Of Medicine | Human enbryonic germ cell and methods of use |
| AU729377B2 (en) | 1997-10-23 | 2001-02-01 | Asterias Biotherapeutics, Inc. | Methods and materials for the growth of primate-derived primordial stem cells in feeder-free culture |
| US6027937A (en) * | 1998-08-18 | 2000-02-22 | Harper; William | Method for propagation of insecta germ cells |
| US6767737B1 (en) * | 1998-08-31 | 2004-07-27 | New York University | Stem cells bearing an FGF receptor on the cell surface |
| US6667176B1 (en) * | 2000-01-11 | 2003-12-23 | Geron Corporation | cDNA libraries reflecting gene expression during growth and differentiation of human pluripotent stem cells |
| US7410798B2 (en) * | 2001-01-10 | 2008-08-12 | Geron Corporation | Culture system for rapid expansion of human embryonic stem cells |
| US20020168766A1 (en) * | 2000-01-11 | 2002-11-14 | Gold Joseph D. | Genetically altered human pluripotent stem cells |
| ATE408667T1 (de) * | 1999-07-20 | 2008-10-15 | Univ Southern California | Identifizierung pluripotenter prämesenchymaler, prähämotopoietischer vorläuferzellen |
| US6333192B1 (en) * | 1999-08-09 | 2001-12-25 | North Carolina State University | Method of producing an undifferentiated avian cell culture using avian primordial germ cells |
| US7455983B2 (en) * | 2000-01-11 | 2008-11-25 | Geron Corporation | Medium for growing human embryonic stem cells |
| US6828145B2 (en) | 2000-05-10 | 2004-12-07 | Cedars-Sinai Medical Center | Method for the isolation of stem cells by immuno-labeling with HLA/MHC gene product marker |
| WO2001091548A2 (en) * | 2000-06-01 | 2001-12-06 | Pharmacia & Upjohn Company | Mice heterozygous for wfs1 gene as mouse models for depression |
| KR100865664B1 (ko) * | 2000-06-14 | 2008-10-29 | 비스타겐 인코포레이티드 | 간 줄기 세포를 사용하는 독성 타이핑 |
| EA013564B1 (ru) * | 2000-08-03 | 2010-06-30 | Терапеутик Хьюман Поликлоналз Инк. | Гуманизированный иммуноглобулин и содержащая его фармацевтическая композиция |
| ATE466089T1 (de) | 2000-12-22 | 2010-05-15 | Agronomique Inst Nat Rech | Positionsunabhängige und gewebespezifische expression eines transgens in der milch eines transgen-tieres |
| CA2435826A1 (en) * | 2001-01-24 | 2002-08-01 | The Government Of The United States Of America | Differentiation of stem cells to pancreatic endocrine cells |
| US20030211605A1 (en) * | 2001-05-01 | 2003-11-13 | Lee Sang-Hun | Derivation of midbrain dopaminergic neurons from embryonic stem cells |
| US7129034B2 (en) * | 2001-10-25 | 2006-10-31 | Cedars-Sinai Medical Center | Differentiation of whole bone marrow |
| US6759244B2 (en) | 2001-11-08 | 2004-07-06 | Art Institute Of New York And New Jersey, Inc. | Composite blastocysts (CBs) from aggregates of dissociated cells of non-viable pre-embryos |
| GB0220145D0 (en) * | 2002-08-30 | 2002-10-09 | Thromb X Nv | Novel compositions for the in vitro derivation and culture of embryonic stem (ES) cell lines with germline transmission capability |
| WO2003054169A1 (en) * | 2001-12-21 | 2003-07-03 | Thromb-X Nv | Compositions for the in vitro derivation and culture of embryonic stem (es) cell lines with germline transmission capability |
| JP4408371B2 (ja) * | 2001-12-21 | 2010-02-03 | トロンボジェニクス・ナムローゼ・フエンノートシャップ | 生殖細胞系伝達能力を有する胚性幹(es)細胞のインビトロの誘導および培養のための組成物 |
| US7145057B2 (en) | 2002-02-01 | 2006-12-05 | Origen Therapeutics, Inc. | Chimeric bird from embryonic stem cells |
| FR2836924B1 (fr) | 2002-03-08 | 2005-01-14 | Vivalis | Lignees de cellules aviaires utiles pour la production de substances d'interet |
| US20030207448A1 (en) * | 2002-05-06 | 2003-11-06 | Revera Gregory Henry | Methodologies for the creation of pluripotent or multipotent human stem cells without creating or destroying a human embryo |
| CA2504179A1 (en) * | 2002-10-31 | 2004-05-13 | Riken | Composition for culturing multipotent stem cells and utilization of the same |
| US8293488B2 (en) | 2002-12-09 | 2012-10-23 | Neuralstem, Inc. | Method for screening neurogenic agents |
| AU2003293409A1 (en) * | 2002-12-09 | 2004-06-30 | Karl K. Johe | Method for discovering neurogenic agents |
| ES2705683T3 (es) * | 2002-12-16 | 2019-03-26 | Technion Res & Dev Foundation | Medio de cultivo de células madre pluripotentes |
| US20040191839A1 (en) * | 2003-03-28 | 2004-09-30 | National Institute Of Agrobiological Sciences Japan | Methods for sorting undifferentiated cells and uses thereof |
| IL155783A (en) | 2003-05-05 | 2010-11-30 | Technion Res & Dev Foundation | Multicellular systems of pluripotent human embryonic stem cells and cancer cells and uses thereof |
| WO2006040615A2 (en) * | 2003-06-11 | 2006-04-20 | Jan Remmereit | Differenciation of stem cells for therapeutic use |
| CA2569978C (en) * | 2004-06-09 | 2017-06-06 | The University Court Of The University Of Edinburgh | Neural stem cells |
| US20050277124A1 (en) * | 2004-06-10 | 2005-12-15 | White Steven M | Cardiac conduction system cells and uses thereof |
| WO2005123900A1 (en) * | 2004-06-10 | 2005-12-29 | Merial Limited | Medium and methods for culturing of avian primordial germ cells |
| WO2006023422A2 (en) * | 2004-08-16 | 2006-03-02 | Kouns George S | Stem-like cells |
| CN101084303A (zh) * | 2004-11-17 | 2007-12-05 | 神经干公司 | 用于治疗神经变性症状的人类神经细胞的移植 |
| CN1298843C (zh) * | 2005-02-07 | 2007-02-07 | 十堰市太和医院 | 不依赖饲养细胞的人胚干细胞培养基 |
| KR20160116024A (ko) * | 2005-06-22 | 2016-10-06 | 아스테리아스 바이오세라퓨틱스, 인크. | 인간 배아 줄기 세포의 현탁 배양 |
| EP2422802A3 (de) | 2006-03-07 | 2013-03-06 | Geeta Shroff | Zusammentsetzungen enhaltend menschliche embryonale Stammzellen und ihre Derivate, Verwendung davon und Verfahren zur ihrer Vorbereitung |
| AU2007285483B2 (en) | 2006-05-11 | 2011-12-22 | Regenics As | Administration of cells and cellular extracts for rejuvenation |
| US20080003676A1 (en) * | 2006-06-26 | 2008-01-03 | Millipore Corporation | Growth of embryonic stem cells |
| EP2500417A1 (de) | 2006-08-09 | 2012-09-19 | Vivalis | Verfahren zur Herstellung von transgenen Vögeln unter Verwendung von embryonalen Stammzellen |
| ATE536374T1 (de) | 2006-09-01 | 2011-12-15 | Therapeutic Human Polyclonals Inc | Erhöhte expression von humanem oder humanisiertem immunglobulin bei nicht-humanen transgenen tieren |
| US8685720B2 (en) | 2006-11-03 | 2014-04-01 | The Trustees Of Princeton University | Engineered cellular pathways for programmed autoregulation of differentiation |
| US20080206864A1 (en) * | 2006-11-22 | 2008-08-28 | Derrick Rancourt | Methods and Materials for Culturing Murine Embryonic Stem Cells |
| US20080267874A1 (en) * | 2007-03-28 | 2008-10-30 | The Buck Institute For Age Research | Targeted Neuronal And Glial Human Embryonic Stem Cell Line |
| EP1985305A1 (de) | 2007-04-24 | 2008-10-29 | Vivalis | Aus Entenembryonen gewonnene Stammzellkulturen zur Herstellung von Impfstoffen gegen Viren |
| CA2723848C (en) | 2008-05-09 | 2013-01-08 | Regenics As | Method of making salmon egg cytoplasmic extracts and use for increasing collagen production in skin |
| US20100158878A1 (en) | 2008-12-23 | 2010-06-24 | Alexandra Capela | Target populations of oligodendrocyte precursor cells and methods of making and using same |
| US8642334B2 (en) | 2009-02-17 | 2014-02-04 | Memorial Sloan Kettering Cancer Center | Methods of neural conversion of human embryonic stem cells |
| CN101962630B (zh) * | 2009-07-23 | 2013-04-10 | 北京大学 | 诱导人胚胎干细胞或人诱导形成的多潜能干细胞向肝细胞分化的方法 |
| AU2011249478B2 (en) | 2010-05-06 | 2014-12-04 | Regenics As | Use of cellular extracts for skin rejuvenation |
| CN103237553A (zh) | 2010-07-28 | 2013-08-07 | 神经干细胞公司 | 治疗和/或逆转神经退行性疾病和/或病症的方法 |
| PT2773748T (pt) | 2011-11-04 | 2020-03-26 | Memorial Sloan Kettering Cancer Center | Neurónios dopaminérgicos (da) do mesencéfalo para enxerto |
| EP3459522B1 (de) | 2012-12-10 | 2021-02-17 | Regenics AS | Verwendung von zellextrakten zur wundbehandlung |
| EP3498824A1 (de) | 2013-04-26 | 2019-06-19 | Memorial Sloan-Kettering Cancer Center | Durch gezielte differenzierung pluripotenter und multipotenter zellen hergestellte kortikale interneuronen und andere neuronale zellen |
| JP6723918B2 (ja) | 2013-11-21 | 2020-07-15 | メモリアル スローン ケタリング キャンサー センター | ヒト多能性幹細胞からの機能的頭蓋プラコード派生体の特定 |
| EP3194623B1 (de) | 2014-08-12 | 2022-01-05 | Wayne State University | Systeme und verfahren zur erkennung von stress von stammzellen und verwendungen davon |
| MX382488B (es) | 2014-10-20 | 2025-03-13 | Neuralstem Inc | Celulas madre neurales estables que comprenden un polinucleótido exógeno que codifica un factor de crecimiento y métodos de uso de las mismas. |
| CN109689089A (zh) | 2016-05-25 | 2019-04-26 | 国家医疗保健研究所 | 治疗癌症的方法和组合物 |
| KR20250008153A (ko) | 2017-11-24 | 2025-01-14 | 인쎄름 (엥스띠뛰 나씨오날 드 라 쌍떼 에 드 라 흐쉐르슈 메디깔) | 암 치료를 위한 방법 및 조성물 |
| WO2020030634A1 (en) | 2018-08-06 | 2020-02-13 | INSERM (Institut National de la Santé et de la Recherche Médicale) | Methods and compositions for treating cancers |
| EP4314246A1 (de) | 2021-04-01 | 2024-02-07 | INSERM (Institut National de la Santé et de la Recherche Médicale) | Verfahren zur herstellung von leberorganoiden, damit erhaltene leberorganoide und verwendungen davon |
Family Cites Families (1)
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| PT87133B (pt) * | 1987-04-02 | 1992-07-31 | Amrad Corp Ltd | Metodo de purificacao do factor inibidor da leucemia (lif) e de composicoes farmaceuticas contendo polipeptidos com actividade do lif |
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1989
- 1989-08-03 JP JP1508674A patent/JP2740320B2/ja not_active Expired - Lifetime
- 1989-08-03 DE DE68928914T patent/DE68928914T2/de not_active Expired - Lifetime
- 1989-08-03 CA CA000607433A patent/CA1341469C/en not_active Expired - Lifetime
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- 1989-08-03 US US07/477,960 patent/US5166065A/en not_active Expired - Lifetime
- 1989-08-03 EP EP89909126A patent/EP0380646B1/de not_active Expired - Lifetime
- 1989-08-03 AU AU40590/89A patent/AU623922B2/en not_active Expired
- 1989-08-03 WO PCT/AU1989/000330 patent/WO1990001541A1/en not_active Ceased
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Also Published As
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|---|---|
| DK17091D0 (da) | 1991-01-31 |
| JPH03503241A (ja) | 1991-07-25 |
| DE68928914D1 (de) | 1999-03-04 |
| JP2740320B2 (ja) | 1998-04-15 |
| HK1003208A1 (en) | 1998-10-16 |
| NO322243B1 (no) | 2006-09-04 |
| AU623922B2 (en) | 1992-05-28 |
| CA1341469C (en) | 2004-12-28 |
| NO910385L (no) | 1991-04-04 |
| EP0380646A4 (en) | 1991-01-23 |
| DK17091A (da) | 1991-04-04 |
| US5166065A (en) | 1992-11-24 |
| AU4059089A (en) | 1990-03-05 |
| ATE175994T1 (de) | 1999-02-15 |
| DE68928914T2 (de) | 1999-09-09 |
| WO1990001541A1 (en) | 1990-02-22 |
| NO910385D0 (no) | 1991-02-01 |
| EP0380646B1 (de) | 1999-01-20 |
| EP0380646A1 (de) | 1990-08-08 |
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