ATE175994T1 - (in vitro)-vermehrung von embryonalen stammzellen unter verwendung von leukämie-inhibitionsfaktor (lif) - Google Patents

(in vitro)-vermehrung von embryonalen stammzellen unter verwendung von leukämie-inhibitionsfaktor (lif)

Info

Publication number
ATE175994T1
ATE175994T1 AT89909126T AT89909126T ATE175994T1 AT E175994 T1 ATE175994 T1 AT E175994T1 AT 89909126 T AT89909126 T AT 89909126T AT 89909126 T AT89909126 T AT 89909126T AT E175994 T1 ATE175994 T1 AT E175994T1
Authority
AT
Austria
Prior art keywords
lif
cells
pct
vitro
propagation
Prior art date
Application number
AT89909126T
Other languages
English (en)
Inventor
Robert Lindsay Williams
Nicholas Martin Gough
Douglas James Hilton
Original Assignee
Amrad Corp Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Amrad Corp Ltd filed Critical Amrad Corp Ltd
Application granted granted Critical
Publication of ATE175994T1 publication Critical patent/ATE175994T1/de

Links

Classifications

    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
    • A01K67/027—New or modified breeds of vertebrates
    • A01K67/0275—Genetically modified vertebrates, e.g. transgenic
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0603—Embryonic cells ; Embryoid bodies
    • C12N5/0606—Pluripotent embryonic cells, e.g. embryonic stem cells [ES]
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00—Genetically modified animals
    • A01K2217/05—Animals comprising random inserted nucleic acids (transgenic)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/20—Cytokines; Chemokines
    • C12N2501/23—Interleukins [IL]
    • C12N2501/235—Leukemia inhibitory factor [LIF]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2510/00—Genetically modified cells

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Environmental Sciences (AREA)
  • Developmental Biology & Embryology (AREA)
  • Gynecology & Obstetrics (AREA)
  • Reproductive Health (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Animal Behavior & Ethology (AREA)
  • Cell Biology (AREA)
  • Microbiology (AREA)
  • Biodiversity & Conservation Biology (AREA)
  • Animal Husbandry (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Veterinary Medicine (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
AT89909126T 1988-08-04 1989-08-03 (in vitro)-vermehrung von embryonalen stammzellen unter verwendung von leukämie-inhibitionsfaktor (lif) ATE175994T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
AUPI964488 1988-08-04

Publications (1)

Publication Number Publication Date
ATE175994T1 true ATE175994T1 (de) 1999-02-15

Family

ID=3773256

Family Applications (1)

Application Number Title Priority Date Filing Date
AT89909126T ATE175994T1 (de) 1988-08-04 1989-08-03 (in vitro)-vermehrung von embryonalen stammzellen unter verwendung von leukämie-inhibitionsfaktor (lif)

Country Status (10)

Country Link
US (1) US5166065A (de)
EP (1) EP0380646B1 (de)
JP (1) JP2740320B2 (de)
AT (1) ATE175994T1 (de)
AU (1) AU623922B2 (de)
CA (1) CA1341469C (de)
DE (1) DE68928914T2 (de)
DK (1) DK176238B1 (de)
NO (1) NO322243B1 (de)
WO (1) WO1990001541A1 (de)

Families Citing this family (99)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20030032178A1 (en) * 1988-08-04 2003-02-13 Williams Robert Lindsay In vitro propagation of embryonic stem cells
EP0453453B1 (de) * 1989-01-10 1996-09-11 Amrad Corporation Limited Leukämiehemmender faktor aus vieharten und seine verwendung zur verbesserung der implantation und der entwicklung von embryonalen zellen
AU622515B2 (en) * 1989-01-10 1992-04-09 Amrad Operations Pty. Limited Leukaemia inhibitory factor from livestock species and use thereof to enhance implantation and development of embryonic cells
US5705732A (en) * 1989-06-12 1998-01-06 Oklahoma Medical Research Foundation Universal donor cells
JP3040815B2 (ja) * 1989-11-24 2000-05-15 モナッシュ・ユニバーシティ 随伴細胞に及ぼす白血病抑制因子の増殖作用
FI924134L (fi) * 1990-03-20 1992-09-16 Amrad Corp Ltd Foerfarande foer reglering av utvecklingen och bevarandet av en neuron
JP3375957B2 (ja) * 1990-07-09 2003-02-10 アムラド・コーポレイション・リミテッド 白血病抑制因子を用いる胚の増強移植、増殖及び維持
EP0613495B1 (de) * 1991-11-14 2005-02-02 WEISSMANN, Charles Transgene nicht-menschliche tiere ohne prionprotein
US7153684B1 (en) * 1992-10-08 2006-12-26 Vanderbilt University Pluripotential embryonic stem cells and methods of making same
US5453357A (en) * 1992-10-08 1995-09-26 Vanderbilt University Pluripotential embryonic stem cells and methods of making same
US5690926A (en) * 1992-10-08 1997-11-25 Vanderbilt University Pluripotential embryonic cells and methods of making same
GB9308271D0 (en) 1993-04-21 1993-06-02 Univ Edinburgh Method of isolating and/or enriching and/or selectively propagating pluripotential animal cells and animals for use in said method
US5523226A (en) * 1993-05-14 1996-06-04 Biotechnology Research And Development Corp. Transgenic swine compositions and methods
EP0716690A1 (de) * 1993-08-30 1996-06-19 Northwestern University Ratte pluripotente embryonale stammzellen und verfahren zu ihrer gewinnung und verwendung
JPH09503673A (ja) * 1993-10-14 1997-04-15 プレジデント・アンド・フエローズ・オブ・ハーバード・カレツジ ニューロン細胞の誘導および維持法
US6686198B1 (en) 1993-10-14 2004-02-03 President And Fellows Of Harvard College Method of inducing and maintaining neuronal cells
US6432711B1 (en) * 1993-11-03 2002-08-13 Diacrin, Inc. Embryonic stem cells capable of differentiating into desired cell lines
GB9401380D0 (en) * 1994-01-25 1994-03-23 Pharmaceutical Proteins Ltd Embryonic cell isolation
FR2726003B1 (fr) 1994-10-21 2002-10-18 Agronomique Inst Nat Rech Milieu de culture de cellules embryonnaires totipotentes aviaires, procede de culture de ces cellules, et cellules embryonnaires totipotentes aviaires
US5874301A (en) * 1994-11-21 1999-02-23 National Jewish Center For Immunology And Respiratory Medicine Embryonic cell populations and methods to isolate such populations
US5843780A (en) * 1995-01-20 1998-12-01 Wisconsin Alumni Research Foundation Primate embryonic stem cells
US5728581A (en) * 1995-06-07 1998-03-17 Systemix, Inc. Method of expanding hematopoietic stem cells, reagents and bioreactors for use therein
WO1997025412A1 (en) 1996-01-09 1997-07-17 The Regents Of The University Of California Ungulate embryonic stem-like cells, methods of making and using the cells to produce transgenic ungulates
WO1997025413A1 (en) 1996-01-09 1997-07-17 The Regents Of The University Of California Ungulate embryonic stem-like cells, making and using the cells to produce a transgenic ungulate
US6247995B1 (en) 1996-02-06 2001-06-19 Bruce Bryan Bioluminescent novelty items
US5876995A (en) 1996-02-06 1999-03-02 Bryan; Bruce Bioluminescent novelty items
JP2001517927A (ja) * 1996-02-28 2001-10-09 バンダービルト ユニバーシティ 胚幹細胞を作製する組成物および方法
AU2892997A (en) * 1996-04-29 1997-11-19 Leuven Research & Development Vzw Pluripotent rabbit embryonic stem (es) cell lines and their use in the generation of chimeric rabbits
US7544511B2 (en) * 1996-09-25 2009-06-09 Neuralstem Biopharmaceuticals Ltd. Stable neural stem cell line methods
AU3392697A (en) * 1996-06-14 1998-01-07 Regents Of The University Of California, The (in vitro) derivation and culture of primate pluripotent stem cells and therapeutic uses thereof
US6416960B1 (en) 1996-08-08 2002-07-09 Prolume, Ltd. Detection and visualization of neoplastic tissues and other tissues
IL129767A0 (en) 1996-12-12 2000-02-29 Prolume Ltd Apparatus and method for detecting and identifying infectious agents
AU745334B2 (en) * 1997-03-06 2002-03-21 Advanced Cell Technology, Inc. Method of cloning animals
US6011197A (en) 1997-03-06 2000-01-04 Infigen, Inc. Method of cloning bovines using reprogrammed non-embryonic bovine cells
US6090622A (en) * 1997-03-31 2000-07-18 The Johns Hopkins School Of Medicine Human embryonic pluripotent germ cells
US6331406B1 (en) 1997-03-31 2001-12-18 The John Hopkins University School Of Medicine Human enbryonic germ cell and methods of use
CA2307807C (en) * 1997-10-23 2008-09-02 Andrea G. Bodnar Methods and materials for the growth of primate-derived primordial stem cells in feeder-free culture
US6027937A (en) * 1998-08-18 2000-02-22 Harper; William Method for propagation of insecta germ cells
US6767737B1 (en) * 1998-08-31 2004-07-27 New York University Stem cells bearing an FGF receptor on the cell surface
US7410798B2 (en) * 2001-01-10 2008-08-12 Geron Corporation Culture system for rapid expansion of human embryonic stem cells
US6667176B1 (en) 2000-01-11 2003-12-23 Geron Corporation cDNA libraries reflecting gene expression during growth and differentiation of human pluripotent stem cells
US20020168766A1 (en) * 2000-01-11 2002-11-14 Gold Joseph D. Genetically altered human pluripotent stem cells
ATE408667T1 (de) * 1999-07-20 2008-10-15 Univ Southern California Identifizierung pluripotenter prämesenchymaler, prähämotopoietischer vorläuferzellen
US6333192B1 (en) 1999-08-09 2001-12-25 North Carolina State University Method of producing an undifferentiated avian cell culture using avian primordial germ cells
US7455983B2 (en) * 2000-01-11 2008-11-25 Geron Corporation Medium for growing human embryonic stem cells
US6828145B2 (en) 2000-05-10 2004-12-07 Cedars-Sinai Medical Center Method for the isolation of stem cells by immuno-labeling with HLA/MHC gene product marker
EP1353549A2 (de) * 2000-06-01 2003-10-22 PHARMACIA & UPJOHN COMPANY Wfs1 heterozygote mäuse als depressionsmuster
KR100865664B1 (ko) * 2000-06-14 2008-10-29 비스타겐 인코포레이티드 간 줄기 세포를 사용하는 독성 타이핑
EA013564B1 (ru) * 2000-08-03 2010-06-30 Терапеутик Хьюман Поликлоналз Инк. Гуманизированный иммуноглобулин и содержащая его фармацевтическая композиция
EP1217071B1 (de) 2000-12-22 2010-04-28 Institut National De La Recherche Agronomique Positionsunabhängige und gewebespezifische Expression eines Transgens in der Milch eines Transgen-Tieres
EP1366148A2 (de) * 2001-01-24 2003-12-03 THE GOVERNMENT OF THE UNITED STATES OF AMERICA, represented by THE DEPARTMENT OF HEALTH & HUMAN SERVICES Differenzierung von stammzellen zu endokrinen pankreaszellen
US20030211605A1 (en) * 2001-05-01 2003-11-13 Lee Sang-Hun Derivation of midbrain dopaminergic neurons from embryonic stem cells
US7129034B2 (en) * 2001-10-25 2006-10-31 Cedars-Sinai Medical Center Differentiation of whole bone marrow
US6759244B2 (en) 2001-11-08 2004-07-06 Art Institute Of New York And New Jersey, Inc. Composite blastocysts (CBs) from aggregates of dissociated cells of non-viable pre-embryos
GB0220145D0 (en) * 2002-08-30 2002-10-09 Thromb X Nv Novel compositions for the in vitro derivation and culture of embryonic stem (ES) cell lines with germline transmission capability
AU2002218893A1 (en) * 2001-12-21 2003-07-09 Thromb-X Nv Compositions for the in vitro derivation and culture of embryonic stem (es) cell lines with germline transmission capability
DE60239420D1 (de) 2001-12-21 2011-04-21 Thrombogenics Nv Zusammensetzungen für die in vitro herleitung und kultur embryonaler stammzellinien mit keimbahnübertragungsfähigkeit und für die kultur adulter stammzellen
US7145057B2 (en) 2002-02-01 2006-12-05 Origen Therapeutics, Inc. Chimeric bird from embryonic stem cells
FR2836924B1 (fr) 2002-03-08 2005-01-14 Vivalis Lignees de cellules aviaires utiles pour la production de substances d'interet
US20030207448A1 (en) * 2002-05-06 2003-11-06 Revera Gregory Henry Methodologies for the creation of pluripotent or multipotent human stem cells without creating or destroying a human embryo
JP4374419B2 (ja) 2002-10-31 2009-12-02 独立行政法人理化学研究所 多能性幹細胞培養用の組成物とその使用
US8293488B2 (en) 2002-12-09 2012-10-23 Neuralstem, Inc. Method for screening neurogenic agents
US20040185429A1 (en) 2002-12-09 2004-09-23 Judith Kelleher-Andersson Method for discovering neurogenic agents
AU2003303071B2 (en) * 2002-12-16 2009-04-23 Technion Research & Development Foundation Ltd. Methods of preparing feeder cells-free, xeno-free human embryonic stem cells and stem cell cultures prepared using same
US20040191839A1 (en) * 2003-03-28 2004-09-30 National Institute Of Agrobiological Sciences Japan Methods for sorting undifferentiated cells and uses thereof
IL155783A (en) 2003-05-05 2010-11-30 Technion Res & Dev Foundation Multicellular systems of pluripotent human embryonic stem cells and cancer cells and uses thereof
EP1702062A2 (de) * 2003-06-11 2006-09-20 Jan Remmereit Kern-umprogrammierung von zellen für die therapeutische verwendung
NZ551832A (en) * 2004-06-09 2010-01-29 Univ Edinburgh Neural stem cells
US20050282273A1 (en) * 2004-06-10 2005-12-22 Swiatek Donald W Ii Medium and methods for culturing of avian primordial germ cells
US20050277124A1 (en) * 2004-06-10 2005-12-15 White Steven M Cardiac conduction system cells and uses thereof
US20090124013A1 (en) * 2004-08-16 2009-05-14 Kouns George S Stem-Like Cells
EP1814979B1 (de) 2004-11-17 2015-04-08 Neuralstem, Inc. Transplantation menschlicher nervenzellen zur behandlung neurodegenerativer erkrankungen
CN1298843C (zh) * 2005-02-07 2007-02-07 十堰市太和医院 不依赖饲养细胞的人胚干细胞培养基
JP5560391B2 (ja) 2005-06-22 2014-07-23 アステリアス バイオセラピューティクス インコーポレイテッド ヒト胚性幹細胞の懸濁培養法
JP5649786B2 (ja) 2006-03-07 2015-01-07 ギータ シュロフ ヒト胚性幹細胞およびそれらの誘導体を含む組成物、使用方法、ならびに調製方法
EP2101724B1 (de) 2006-05-11 2020-12-02 Regenics AS Verabreichung von zellextrakten zur verjüngung
US20080003676A1 (en) * 2006-06-26 2008-01-03 Millipore Corporation Growth of embryonic stem cells
MX2009001477A (es) 2006-08-09 2009-02-18 Vivalis Metodo de produccion de un aviar transgenico utilizando celulas progenitoras embrionicas.
PE20081216A1 (es) 2006-09-01 2008-09-04 Therapeutic Human Polyclonals Inc Expresion mejorada de la inmunoglobulina humana o humanizada en animales transgenicos no humanos
WO2008066658A2 (en) * 2006-11-03 2008-06-05 The Trustees Of Princeton University Engineered cellular pathways for programmed autoregulation of differentiation
US20080206864A1 (en) * 2006-11-22 2008-08-28 Derrick Rancourt Methods and Materials for Culturing Murine Embryonic Stem Cells
US20080267874A1 (en) * 2007-03-28 2008-10-30 The Buck Institute For Age Research Targeted Neuronal And Glial Human Embryonic Stem Cell Line
EP1985305A1 (de) 2007-04-24 2008-10-29 Vivalis Aus Entenembryonen gewonnene Stammzellkulturen zur Herstellung von Impfstoffen gegen Viren
CA2723848C (en) 2008-05-09 2013-01-08 Regenics As Method of making salmon egg cytoplasmic extracts and use for increasing collagen production in skin
EP3187581A1 (de) 2008-12-23 2017-07-05 BOCO Silicon Valley, Inc. Zielpopulationen von oligodendrozyten-vorläuferzellen und verfahren zur herstellung und verwendung davon
WO2010096496A2 (en) 2009-02-17 2010-08-26 Memorial Sloan-Kettering Cancer Center Methods of neural conversion of human embryonic stem cells
CN101962630B (zh) * 2009-07-23 2013-04-10 北京大学 诱导人胚胎干细胞或人诱导形成的多潜能干细胞向肝细胞分化的方法
EP2566583B1 (de) 2010-05-06 2018-07-11 Regenics AS Verfahren zur Vorbereitung eines Fischeizellextrakts
NZ623207A (en) 2010-07-28 2014-12-24 Neuralstem Inc Methods for treating and/or reversing neurodegenerative diseases and/or disorders
EP3693455A1 (de) 2011-11-04 2020-08-12 Memorial Sloan-Kettering Cancer Center Mittelhirn-dopamin (da)-neuronen für transplantation
EP3459522B1 (de) 2012-12-10 2021-02-17 Regenics AS Verwendung von zellextrakten zur wundbehandlung
EP3498824A1 (de) 2013-04-26 2019-06-19 Memorial Sloan-Kettering Cancer Center Durch gezielte differenzierung pluripotenter und multipotenter zellen hergestellte kortikale interneuronen und andere neuronale zellen
AU2014352773B2 (en) 2013-11-21 2021-04-29 Memorial Sloan-Kettering Cancer Center Specification of functional cranial placode derivatives from human pluripotent stem cells
EP3194623B1 (de) 2014-08-12 2022-01-05 Wayne State University Systeme und verfahren zur erkennung von stress von stammzellen und verwendungen davon
WO2016064737A1 (en) 2014-10-20 2016-04-28 Neuralstem, Inc. Stable neural stem cells comprising an exogenous polynucleotide coding for a growth factor and methods of use thereof
CN116376812A (zh) 2016-05-25 2023-07-04 国家医疗保健研究所 治疗癌症的方法和组合物
JP7408036B2 (ja) 2017-11-24 2024-01-05 アンスティチュ ナショナル ドゥ ラ サンテ エ ドゥ ラ ルシェルシュ メディカル ガンを処置するための方法及び組成物
CN112703011A (zh) 2018-08-06 2021-04-23 国家医疗保健研究所 用于治疗癌症的方法和组合物
EP4314246A1 (de) 2021-04-01 2024-02-07 INSERM (Institut National de la Santé et de la Recherche Médicale) Verfahren zur herstellung von leberorganoiden, damit erhaltene leberorganoide und verwendungen davon

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
PT87133B (pt) * 1987-04-02 1992-07-31 Amrad Corp Ltd Metodo de purificacao do factor inibidor da leucemia (lif) e de composicoes farmaceuticas contendo polipeptidos com actividade do lif

Also Published As

Publication number Publication date
US5166065A (en) 1992-11-24
NO322243B1 (no) 2006-09-04
AU4059089A (en) 1990-03-05
JP2740320B2 (ja) 1998-04-15
HK1003208A1 (en) 1998-10-16
EP0380646A1 (de) 1990-08-08
CA1341469C (en) 2004-12-28
DE68928914T2 (de) 1999-09-09
DE68928914D1 (de) 1999-03-04
WO1990001541A1 (en) 1990-02-22
JPH03503241A (ja) 1991-07-25
DK176238B1 (da) 2007-04-10
DK17091D0 (da) 1991-01-31
AU623922B2 (en) 1992-05-28
NO910385D0 (no) 1991-02-01
EP0380646B1 (de) 1999-01-20
EP0380646A4 (en) 1991-01-23
NO910385L (no) 1991-04-04
DK17091A (da) 1991-04-04

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