DK1891205T3 - Suspensionskulturbeholdere - Google Patents
Suspensionskulturbeholdere Download PDFInfo
- Publication number
- DK1891205T3 DK1891205T3 DK06772571.3T DK06772571T DK1891205T3 DK 1891205 T3 DK1891205 T3 DK 1891205T3 DK 06772571 T DK06772571 T DK 06772571T DK 1891205 T3 DK1891205 T3 DK 1891205T3
- Authority
- DK
- Denmark
- Prior art keywords
- culture
- volume
- vessel
- conical bottom
- wide
- Prior art date
Links
- 238000004114 suspension culture Methods 0.000 title description 11
- 238000004113 cell culture Methods 0.000 claims abstract description 15
- 239000001963 growth medium Substances 0.000 claims description 22
- 238000000034 method Methods 0.000 claims description 6
- 238000012258 culturing Methods 0.000 claims description 3
- 239000012530 fluid Substances 0.000 claims 3
- 238000011218 seed culture Methods 0.000 claims 2
- 239000012679 serum free medium Substances 0.000 claims 1
- 210000004027 cell Anatomy 0.000 description 18
- 239000002609 medium Substances 0.000 description 17
- 238000005273 aeration Methods 0.000 description 13
- 239000000523 sample Substances 0.000 description 9
- 238000004519 manufacturing process Methods 0.000 description 8
- 238000013341 scale-up Methods 0.000 description 8
- 241001465754 Metazoa Species 0.000 description 7
- 210000004962 mammalian cell Anatomy 0.000 description 7
- 230000005587 bubbling Effects 0.000 description 6
- 239000000463 material Substances 0.000 description 6
- 239000000725 suspension Substances 0.000 description 6
- 238000012360 testing method Methods 0.000 description 6
- 230000009194 climbing Effects 0.000 description 5
- 238000005457 optimization Methods 0.000 description 5
- 241000699802 Cricetulus griseus Species 0.000 description 4
- 238000013461 design Methods 0.000 description 4
- 230000000977 initiatory effect Effects 0.000 description 4
- 102000007056 Recombinant Fusion Proteins Human genes 0.000 description 3
- 108010008281 Recombinant Fusion Proteins Proteins 0.000 description 3
- 238000004458 analytical method Methods 0.000 description 3
- 238000010923 batch production Methods 0.000 description 3
- 210000004978 chinese hamster ovary cell Anatomy 0.000 description 3
- JKMHFZQWWAIEOD-UHFFFAOYSA-N 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid Chemical compound OCC[NH+]1CCN(CCS([O-])(=O)=O)CC1 JKMHFZQWWAIEOD-UHFFFAOYSA-N 0.000 description 2
- 239000007995 HEPES buffer Substances 0.000 description 2
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 2
- 230000005779 cell damage Effects 0.000 description 2
- 208000037887 cell injury Diseases 0.000 description 2
- 238000005119 centrifugation Methods 0.000 description 2
- 238000011161 development Methods 0.000 description 2
- 230000018109 developmental process Effects 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 230000012010 growth Effects 0.000 description 2
- 238000010899 nucleation Methods 0.000 description 2
- 229910052760 oxygen Inorganic materials 0.000 description 2
- 239000001301 oxygen Substances 0.000 description 2
- 238000001556 precipitation Methods 0.000 description 2
- 108090000623 proteins and genes Proteins 0.000 description 2
- 238000012216 screening Methods 0.000 description 2
- 108010089254 Cholesterol oxidase Proteins 0.000 description 1
- 241000223782 Ciliophora Species 0.000 description 1
- 238000010564 aerobic fermentation Methods 0.000 description 1
- 238000013019 agitation Methods 0.000 description 1
- 230000000259 anti-tumor effect Effects 0.000 description 1
- 238000013459 approach Methods 0.000 description 1
- 239000000872 buffer Substances 0.000 description 1
- 238000004364 calculation method Methods 0.000 description 1
- 238000002659 cell therapy Methods 0.000 description 1
- 230000000052 comparative effect Effects 0.000 description 1
- 230000001419 dependent effect Effects 0.000 description 1
- 238000011038 discontinuous diafiltration by volume reduction Methods 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 238000005187 foaming Methods 0.000 description 1
- 238000001415 gene therapy Methods 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 231100000331 toxic Toxicity 0.000 description 1
- 230000002588 toxic effect Effects 0.000 description 1
- 230000014616 translation Effects 0.000 description 1
- 229960005486 vaccine Drugs 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
- C12M27/16—Vibrating; Shaking; Tilting
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/08—Flask, bottle or test tube
Landscapes
- Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Organic Chemistry (AREA)
- Chemical & Material Sciences (AREA)
- Zoology (AREA)
- Biomedical Technology (AREA)
- Sustainable Development (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Biotechnology (AREA)
- Clinical Laboratory Science (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Claims (5)
1. Cellekultur-beholder omfattende et hus, der definerer et kammer, der inkluderer en inverteret keglestubs-bund og et cylindrisk legeme, der strækker sig opad fra den inverterede keglestubs-bund, hvor kammeret har en vertikal akse, hvor den inverterede keglestubs-bund har en hældning, en øverste del og en modstående nederste del, hvilken nederste del har en større indre diameter end den øverste del, og hvor dimensionerne af den indre diameter af den øverste del, af den indre diameter af den nederste del, af den indre diameter af det cylindriske legeme, af højden af den inverterede keglestubs-bund og af højden af det cylindriske legeme er henholdsvis 40 mm, 250 mm, 250 mm, 90 mm og 110 mm.
2. System til dyrkning af celler, omfattende en beholder ifølge krav 1 og et orbital rystebord eller impellere.
3. Fremgangsmåde til at dyrke levende celler, omfattende trinnene: (a) at tilvejebringe en beholder ifølge krav 1; (b) at indføre et arbejdsvæske-dyrkningsmedium til beholderen; (c) at indføre til beholderen en podekultur indeholdende celler af interesse; og (d) at dyrke cellerne under en egnet betingelse, hvor forholdet mellem det 02-åndbare areal/kulturmediumvolumenet ikke er mindre end 0,14 cm2/cm3.
4. Fremgangsmåden ifølge krav 3, hvor forholdet mellem arbejdsvæske-dyrkningsmedium-volumen/podekultur-volumen ikke er større end 1/10.
5. Fremgangsmåden ifølge krav 3, hvor arbejdsvæske-dyrkningsmediet indeholder et serum-frit medium med C02-ikke-afhængigt 20 mM FIEPES eller ækvivalenter for at fastholde pH af kulturen stabil.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US69058705P | 2005-06-15 | 2005-06-15 | |
| PCT/US2006/022312 WO2006138143A1 (en) | 2005-06-15 | 2006-06-08 | Suspension culture vessels |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| DK1891205T3 true DK1891205T3 (da) | 2018-03-19 |
Family
ID=56290828
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DK06772571.3T DK1891205T3 (da) | 2005-06-15 | 2006-06-08 | Suspensionskulturbeholdere |
Country Status (5)
| Country | Link |
|---|---|
| US (1) | US20080199958A1 (da) |
| EP (1) | EP1891205B1 (da) |
| JP (1) | JP2008543307A (da) |
| DK (1) | DK1891205T3 (da) |
| ES (1) | ES2662589T3 (da) |
Families Citing this family (18)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| SI2251407T1 (sl) * | 2009-05-12 | 2016-09-30 | Eppendorf Ag | Bioreaktor za enkratno uporabo in postopek za njegovo proizvodnjo |
| JP2013543372A (ja) * | 2010-07-29 | 2013-12-05 | ビオメリュー・インコーポレイテッド | 内部センサを備えた培養ボトル |
| USD684275S1 (en) | 2012-01-25 | 2013-06-11 | Biomerieux, Inc. | Specimen container |
| SG10201901708SA (en) * | 2012-10-23 | 2019-03-28 | Genzyme Corp | Perfusion culturing methods and uses thereof |
| CN105143443A (zh) | 2013-02-22 | 2015-12-09 | 建新公司 | 微载体灌注培养方法及其用途 |
| CN105209603A (zh) | 2013-02-22 | 2015-12-30 | 建新公司 | 微载体灌注培养方法及其用途 |
| JP6859102B2 (ja) * | 2014-04-10 | 2021-04-14 | 株式会社フォーシスアンドカンパニー | ８角柱の細胞培養用容器 |
| TWI743024B (zh) | 2014-06-06 | 2021-10-21 | 美商健臻公司 | 灌注培養方法及其用途 |
| TWI776235B (zh) | 2014-06-09 | 2022-09-01 | 美商健臻公司 | 種子罐培養法(seed train processes)及其用途 |
| KR102769649B1 (ko) | 2014-12-22 | 2025-02-19 | 젠자임 코포레이션 | 포유류 세포의 배양 방법 |
| JP6566549B2 (ja) * | 2015-04-23 | 2019-08-28 | 藤森工業株式会社 | 培養装置を用いた培養方法 |
| KR102681292B1 (ko) * | 2016-06-24 | 2024-07-03 | 론자 리미티드 | 가변 직경 생물 반응기 |
| CN107760604A (zh) * | 2017-10-24 | 2018-03-06 | 江门市茵华美生物科技有限公司 | 微量悬浮细胞培养反应器 |
| EP3784771A1 (en) * | 2018-04-25 | 2021-03-03 | Global Life Sciences Solutions USA LLC | Bioreactors |
| US20220002653A1 (en) * | 2018-11-07 | 2022-01-06 | Bluebird Bio, Inc. | Cell therapy vessels |
| CN115369091B (zh) * | 2022-09-29 | 2023-07-28 | 成都赛诺联创生物科技有限公司 | 一种Caco-2细胞倒置模型及其制备方法 |
| EP4642889A2 (en) * | 2022-12-30 | 2025-11-05 | Life Technologies Corporation | Cell culture flask |
| CN116286354A (zh) * | 2023-03-24 | 2023-06-23 | 浙江工业大学 | 一种椭圆形振荡式生物反应器 |
Family Cites Families (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE1773331A1 (de) * | 1968-05-02 | 1972-01-27 | Eppendorf Geraetebau Netheler | Reaktionsgefaess fuer kleine Fluessigkeitsmengen |
| JPS5924657B2 (ja) * | 1977-08-02 | 1984-06-11 | 東京理化器械株式会社 | 気液接触式反応装置 |
| JPS60177800U (ja) * | 1984-05-08 | 1985-11-26 | 日東電工株式会社 | 無菌接種装置 |
| US5372945A (en) * | 1985-06-06 | 1994-12-13 | Alchas; Paul G. | Device and method for collecting and processing fat tissue and procuring microvessel endothelial cells to produce endothelial cell product |
| US4665035A (en) * | 1986-05-27 | 1987-05-12 | Josephino Tunac | Fermentation apparatus and systems for the cultivation of microorganisms and other biological entities |
| JPH0281200U (da) * | 1988-07-13 | 1990-06-22 | ||
| US5320963A (en) * | 1992-11-25 | 1994-06-14 | National Research Council Of Canada | Bioreactor for the perfusion culture of cells |
| US5447866A (en) * | 1994-02-02 | 1995-09-05 | Eco Soil Systems | Reactor for microorganisms and feed device therefor |
| DE19524307A1 (de) * | 1995-07-07 | 1997-01-09 | Hoechst Ag | Verfahren zur Massenkultivierung von Ciliaten |
| IL119310A (en) * | 1996-09-26 | 1999-07-14 | Metabogal Ltd | Cell/tissue culturing device and method |
| WO1998027195A1 (de) * | 1996-12-18 | 1998-06-25 | Roche Diagnostics Gmbh | Zellkulturgefäss und verwendung von multischalen zur züchtung von antitumorzellen |
| AU6021699A (en) * | 1998-09-01 | 2000-03-21 | Penn State Research Foundation | Method and apparatus for aseptic growth or processing of biomass |
| US6991933B1 (en) * | 1999-09-24 | 2006-01-31 | Cytomatrix, Llc | Cell culture spinner flasks |
-
2006
- 2006-06-08 DK DK06772571.3T patent/DK1891205T3/da active
- 2006-06-08 ES ES06772571.3T patent/ES2662589T3/es not_active Expired - Lifetime
- 2006-06-08 JP JP2008516944A patent/JP2008543307A/ja active Pending
- 2006-06-08 EP EP06772571.3A patent/EP1891205B1/en not_active Expired - Lifetime
- 2006-06-08 US US11/916,707 patent/US20080199958A1/en not_active Abandoned
Also Published As
| Publication number | Publication date |
|---|---|
| EP1891205A4 (en) | 2012-06-06 |
| ES2662589T3 (es) | 2018-04-09 |
| EP1891205B1 (en) | 2017-12-27 |
| EP1891205A1 (en) | 2008-02-27 |
| US20080199958A1 (en) | 2008-08-21 |
| JP2008543307A (ja) | 2008-12-04 |
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