DK1994141T3 - Sammensætninger og fremgangsmåder anvendelige til dyrkning af differentierbare celler - Google Patents

Sammensætninger og fremgangsmåder anvendelige til dyrkning af differentierbare celler Download PDF

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DK1994141T3
DK1994141T3 DK07757440.8T DK07757440T DK1994141T3 DK 1994141 T3 DK1994141 T3 DK 1994141T3 DK 07757440 T DK07757440 T DK 07757440T DK 1994141 T3 DK1994141 T3 DK 1994141T3
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cells
cell
insulin
methods
composition
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Allan Robins
Thomas Schulz
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Viacyte Inc
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Claims (11)

  1. SAMMENSÆTNINGER OG FREMGANGSMÅDER ANVENDELIGE TIL DYRKNING AF DIEFERENTIERBARE CELLER
    1. Anvendelse af en sammensætning til dyrkning og opretholdelse af pluripotente differeniierbare celler i en stabiliseret pluripotent tilstand, hvilken sammensætning omfatter en basal saitnæringsopløsning, en ErbB3-ligand, en aktivator af insulin-lignende vækstfaktor 1 receptor (IGF1R) og indeholder mindre end 3% serum, hvor anvendelsen sker i fravær af et næringslag, hvor (a) nævnte ErbS3-!igand er udvalgt fra gruppen bestående af Neuregulin-1, Heregulin-β (HRG-β), Heregulin-a (HRG-a), Neu-differentieringsfaktor (NDF), acetylcholinreeeptor-inducerende aktivitet (ARIA), glialvækstfaktor 2 (GGF2), motor-neuron-afiedt faktor (SMDF), Neuregulin-2, Neuregu!in-2(i (NRG2-p), Kpuegnlin, Biregulin og funktionelle fragmenter deraf, især foretrukket HRG-β eller et funktionelt fragment deraf; og (b) hvor aktivatoren af IGF1R omfatter en insulin-lignende vækstfaktor eller et funktionelt fragment deraf.
  2. 2. Anvendelse af en sammensætning ifølge krav 1, hvor sammensætningen er fri for eksogent insulin og insulin-substitutter.
  3. 3. Anvendelse af en sammensætning ifølge krav 1 eller 2, hvor sammensætningen endvidere omfatter transformeringsvækstfaktor beta (TGF-β), et TGFP-familiemedlem eller et funktionelt fragment deraf, hvor TGF-P-familiemedlemmet fortrinsvis er udvalgt fra gruppen bestående af Nodal, Activin A, Activin B, knoglemorfogent protein-2 (BMP2), knoglemorfogent protein-4 (BMP4) og funktionelle fragmenter deraf, især Activin A.
  4. 4. Anvendelse af en sammensætning ifølge et hvilket som helst af de foregående krav, hvor sammensætningen er fri for eksogen fibroblastvækstfaktor eller endvidere omfatter mindst en fibroblasivækstfalctor (FGF) udvalgt fra gruppen bestående af FGF-2, FGF-7, FGF-10, FGF-22 og funktionelle fragmenter deraf.
  5. 5. Anvendelse af en sammensætning ifølge et hvilket som helst af de foregående krav, hvor sammensætningen endvidere omfatter et serumalbumin (SA), fortrinsvis bovint SA (BSA) eller human SA (I-ISA), og hvor koncentrationen af SA fortrinsvis er mere end ca. 0,2%, volumen til volumen (vol/vol) og mere fortrinsvis mindre end ca. .5 volumenprocent.
  6. 6. Anvendelse, af en sammensætning ifølge et hvilket som helst af de foregående krav, hvor sammensætningen endvidere omfatter mindst ét uopløseligt substrat, fortrinsvis udvalgt fra gruppen bestående af et collagen, et fibroneetin og fragmenter deraf.
  7. 7. Anvendelse af en sammensætning ifølge et hvilket som helst af de foregående, krav, hvor den insulin-lignende vækstfaktor er insulin-lignende vækstfaktor 1 (IGF!) eller lougR3-IGFL
  8. 8. Anvendelse af en sammensætning ifølge et hvilket som helst af de foregående krav, hvor sammensætningen indeholder mindre end 2% serum.
  9. 9. Anvendelse af en sammensætning ifølge ei hvilket som helst af de foregående krav, hvor sammensætningen indeholder mindre end 1% serum.
  10. 10. Anvendelse af en sammensætning ifølge et hvilket som helst af de foregående krav, hvor sammensætningen ikke indeholder serum.
  11. 11. Anvendelse af en sammensætning ifølge et hvilket som helst af de foregående krav, hvor sammensætningen ikke indeholder serumerstatning.
DK07757440.8T 2006-02-23 2007-02-23 Sammensætninger og fremgangsmåder anvendelige til dyrkning af differentierbare celler DK1994141T3 (da)

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US77611306P 2006-02-23 2006-02-23
PCT/US2007/062755 WO2007101130A2 (en) 2006-02-23 2007-02-23 Compositions and methods useful for culturing differentiable cells

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US (6) US8153429B2 (da)
EP (2) EP1994141B1 (da)
JP (3) JP5830217B2 (da)
KR (1) KR20080109775A (da)
CN (3) CN101410509B (da)
AU (1) AU2007220759A1 (da)
CA (1) CA2643478C (da)
DK (2) DK1994141T3 (da)
SG (3) SG170021A1 (da)
WO (1) WO2007101130A2 (da)

Families Citing this family (110)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7097840B2 (en) 2000-03-16 2006-08-29 Genentech, Inc. Methods of treatment using anti-ErbB antibody-maytansinoid conjugates
WO2003014313A2 (en) * 2001-08-06 2003-02-20 Bresagen, Ltd. Alternative compositions and methods for the culture of stem cells
US8187878B2 (en) 2004-08-13 2012-05-29 University Of Georgia Research Foundation, Inc. Methods for increasing definitive endoderm differentiation of pluripotent human embryonic stem cells with PI-3 kinase inhibitors
US8017395B2 (en) 2004-12-17 2011-09-13 Lifescan, Inc. Seeding cells on porous supports
JP2009500297A (ja) 2005-06-08 2009-01-08 セントカー・インコーポレーテツド 眼の変性についての細胞療法
SG170021A1 (en) 2006-02-23 2011-04-29 Novocell Inc Compositions and methods useful for culturing differentiable cells
US8741643B2 (en) 2006-04-28 2014-06-03 Lifescan, Inc. Differentiation of pluripotent stem cells to definitive endoderm lineage
US7964402B2 (en) 2006-05-25 2011-06-21 Sanford-Burnham Medical Research Institute Methods for culture and production of single cell populations of human embryonic stem cells
JP2008099662A (ja) 2006-09-22 2008-05-01 Institute Of Physical & Chemical Research 幹細胞の培養方法
EP2559756B1 (en) * 2007-07-01 2019-12-18 Janssen Biotech, Inc. Single pluripotent stem cell culture
US9080145B2 (en) 2007-07-01 2015-07-14 Lifescan Corporation Single pluripotent stem cell culture
DK2185693T3 (da) 2007-07-31 2019-09-23 Lifescan Inc Differentiering af humane embryoniske stamceller
US8623650B2 (en) * 2007-10-19 2014-01-07 Viacyte, Inc. Methods and compositions for feeder-free pluripotent stem cell media containing human serum
CA2954431C (en) 2007-11-27 2021-08-24 Lifescan, Inc. Differentiation of human embryonic stem cells to pancreatic cells
JP5733986B2 (ja) 2008-02-21 2015-06-10 ヤンセン バイオテツク，インコーポレーテツド 細胞の付着、培養、及び剥離のための方法、表面改質されたプレート、並びに組成物
US8338170B2 (en) 2008-04-21 2012-12-25 Viacyte, Inc. Methods for purifying endoderm and pancreatic endoderm cells derived from human embryonic stem cells
US8623648B2 (en) 2008-04-24 2014-01-07 Janssen Biotech, Inc. Treatment of pluripotent cells
EP2128245A1 (en) * 2008-05-27 2009-12-02 Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V. Generation of induced pluripotent stem (iPS) cells
AU2009267167A1 (en) * 2008-06-30 2010-01-07 Centocor Ortho Biotech Inc. Differentiation of pluripotent stem cells
EP2942392B1 (en) 2008-06-30 2018-10-03 Janssen Biotech, Inc. Differentiation of pluripotent stem cells
EP3777878A1 (en) * 2008-08-15 2021-02-17 Acorda Therapeutics, Inc. Compositions and methods for treatment during non-acute periods following cns neurological injury
EP2350265B1 (en) 2008-10-31 2019-04-17 Janssen Biotech, Inc. Differentiation of human embryonic stem cells to the pancreatic endocrine lineage
US9234178B2 (en) 2008-10-31 2016-01-12 Janssen Biotech, Inc. Differentiation of human pluripotent stem cells
US8895300B2 (en) 2008-11-04 2014-11-25 Viacyte, Inc. Scalable primate pluripotent stem cell aggregate suspension culture and differentiation thereof
JP5390624B2 (ja) * 2008-11-04 2014-01-15 バイアサイト インク 幹細胞集合体懸濁液組成物、その分化方法
US8008075B2 (en) * 2008-11-04 2011-08-30 Viacyte, Inc. Stem cell aggregate suspension compositions and methods of differentiation thereof
JP2012508584A (ja) 2008-11-14 2012-04-12 ヴィアサイト，インコーポレイテッド ヒト多能性幹細胞由来膵臓細胞のカプセル化
KR101687344B1 (ko) 2008-11-20 2016-12-16 얀센 바이오테크 인코포레이티드 평면 기재상의 세포 부착 및 배양을 위한 방법 및 조성물
KR101774546B1 (ko) 2008-11-20 2017-09-04 얀센 바이오테크 인코포레이티드 마이크로-캐리어 상의 만능 줄기 세포 배양
EP2393917B1 (en) * 2009-02-03 2016-04-06 Koninklijke Nederlandse Akademie van Wetenschappen Culture medium for epithelial stem cells and organoids comprising said stem cells.
EP2412800A1 (en) 2010-07-29 2012-02-01 Koninklijke Nederlandse Akademie van Wetenschappen Liver organoid, uses thereof and culture method for obtaining them
US9752124B2 (en) 2009-02-03 2017-09-05 Koninklijke Nederlandse Akademie Van Wetenschappen Culture medium for epithelial stem cells and organoids comprising the stem cells
US9109245B2 (en) 2009-04-22 2015-08-18 Viacyte, Inc. Cell compositions derived from dedifferentiated reprogrammed cells
CN107603943B (zh) * 2009-04-27 2019-10-11 威盛细胞有限公司 支持多能细胞生长的小分子和其方法
CN102482643B (zh) 2009-07-20 2016-06-29 詹森生物科技公司 人胚胎干细胞的分化
CN103952372B (zh) 2009-07-20 2016-10-05 詹森生物科技公司 人胚胎干细胞的分化
US8785184B2 (en) 2009-07-20 2014-07-22 Janssen Biotech, Inc. Differentiation of human embryonic stem cells
JP5902092B2 (ja) * 2009-10-19 2016-04-13 セルラー ダイナミクス インターナショナル， インコーポレイテッド 心筋細胞の生成
KR101135636B1 (ko) * 2009-10-27 2012-04-17 서울대학교산학협력단 인간 만능줄기세포로부터 중배엽 줄기세포를 생산하는 방법 및 이에 의해 생성된 중배엽 줄기세포
US9018010B2 (en) * 2009-11-12 2015-04-28 Technion Research & Development Foundation Limited Culture media, cell cultures and methods of culturing pluripotent stem cells in an undifferentiated state
CN102712902B (zh) 2009-12-23 2019-01-08 詹森生物科技公司 人胚胎干细胞的分化
SG10201408552YA (en) * 2009-12-23 2015-02-27 Janssen Biotech Inc Differentiation of human embryonic stem cells
PH12012501686A1 (en) 2010-03-01 2020-10-19 Janssen Biotech Inc Methods for purifying cells derived from pluripotent stem cells
RU2587634C2 (ru) 2010-05-12 2016-06-20 Янссен Байотек, Инк. Дифференцирование эмбриональных стволовых клеток человека
PL2853589T3 (pl) 2010-08-31 2018-05-30 Janssen Biotech, Inc Różnicowanie ludzkich zarodkowych komórek macierzystych
EP3372672A1 (en) 2010-08-31 2018-09-12 Janssen Biotech, Inc. Differentiation of human embryonic stem cells
CN103154237B (zh) 2010-08-31 2016-03-16 詹森生物科技公司 多能干细胞的分化
US9181529B2 (en) 2010-10-19 2015-11-10 Cellular Dynamics International, Inc. Titration of differentiation medium components
BR112013017629A2 (pt) * 2010-12-17 2017-04-18 Biolamina Ab laminina-521 recombinante
JP5890100B2 (ja) * 2011-02-09 2016-03-22 雪印メグミルク株式会社 皮膚コラーゲン産生促進剤
US9433557B2 (en) 2011-02-21 2016-09-06 Viacyte, Inc. Loading system for an encapsulation device
DK2694644T3 (da) 2011-03-30 2018-04-16 Cellular Dynamics Int Inc Priming af pluripotente stamceller til neural differentiering
CN102294053B (zh) * 2011-07-24 2014-10-29 陕西省眼科研究所 去细胞异种角膜基质载体及其制备方法和应用
JP5859543B2 (ja) * 2011-08-03 2016-02-10 扶桑薬品工業株式会社 胚培養用組成物
AU2012335070A1 (en) 2011-11-11 2014-05-29 Bio-Ess Laboratories, Llc Kit comprising serum replacement and labile factors
FI2794857T3 (fi) 2011-12-22 2026-03-25 Janssen Biotech Inc Ihmisen alkion kantasolujen erilaistaminen yksittäisiksi hormonaalisiksi insuliinipositiivisiksi soluiksi
CA2866590A1 (en) 2012-03-07 2013-09-12 Janssen Biotech, Inc. Defined media for expansion and maintenance of pluripotent stem cells
EP3450544A1 (en) 2012-06-08 2019-03-06 Janssen Biotech, Inc. Differentiation of human embryonic stem cells into pancreatic endodrine cells
AU2013248265B2 (en) 2012-11-08 2018-11-01 Viacyte, Inc. Scalable primate pluripotent stem cell aggregate suspension culture and differentiation thereof
DK2938723T3 (da) 2012-12-31 2023-02-20 Janssen Biotech Inc Differentiering af humane embryonale stamceller til pancreatiske endokrine celler under anvendelse af hb9-regulatorer
US10370644B2 (en) 2012-12-31 2019-08-06 Janssen Biotech, Inc. Method for making human pluripotent suspension cultures and cells derived therefrom
ES2837763T3 (es) 2012-12-31 2021-07-01 Janssen Biotech Inc Cultivo de células madre embrionarias humanas en la interconexión aire-líquido para la diferenciación en células endocrinas pancreáticas
BR112015015714A2 (pt) 2012-12-31 2017-07-11 Janssen Biotech Inc suspensão e aglomeração de células pluripotentes humanas para diferenciação em célu-las endócrinas pancreáticas
AU2014225352B2 (en) 2013-03-07 2017-02-09 Viacyte, Inc. 3-dimensional large capacity cell encapsulation device assembly
USD720469S1 (en) 2013-03-07 2014-12-30 Viacyte, Inc. Cell encapsulation device
US8859286B2 (en) 2013-03-14 2014-10-14 Viacyte, Inc. In vitro differentiation of pluripotent stem cells to pancreatic endoderm cells (PEC) and endocrine cells
SG11201509767UA (en) 2013-05-30 2015-12-30 Ajinomoto Kk Medium for culturing stem cells
CN120796168A (zh) 2013-06-11 2025-10-17 哈佛学院校长同事会 SC-β细胞以及用于产生其的组合物和方法
US9527401B2 (en) * 2014-01-23 2016-12-27 Johnson Controls Technology Company Semi-active architectures for batteries having two different chemistries
JP2014036660A (ja) * 2013-10-09 2014-02-27 Viacyte Inc 幹細胞集合体懸濁液組成物、その分化方法
KR20160125352A (ko) 2013-12-20 2016-10-31 이센셜 파마수티컬스 엘엘씨 세포 배양용 배지
USD734483S1 (en) * 2013-12-31 2015-07-14 Alamak Biosciences Incorporation Company Limited Tissue cell block for cancer detection
KR102288576B1 (ko) * 2014-03-31 2021-08-12 아지노모토 가부시키가이샤 줄기세포용 배지
US11051900B2 (en) 2014-04-16 2021-07-06 Viacyte, Inc. Tools and instruments for use with implantable encapsulation devices
CA2949171A1 (en) 2014-05-16 2015-11-19 Koninklijke Nederlandse Akademie Van Wetenschappen Improved culture method for organoids
WO2015175307A1 (en) 2014-05-16 2015-11-19 Janssen Biotech, Inc. Use of small molecules to enhance mafa expression in pancreatic endocrine cells
GB201421092D0 (en) 2014-11-27 2015-01-14 Koninklijke Nederlandse Akademie Van Wetenschappen Culture medium
GB201421094D0 (en) 2014-11-27 2015-01-14 Koninklijke Nederlandse Akademie Van Wetenschappen Culture medium
EP3234110B1 (en) 2014-12-18 2024-02-28 President and Fellows of Harvard College METHODS FOR GENERATING STEM CELL-DERIVED ß CELLS AND USES THEREOF
WO2016100898A1 (en) 2014-12-18 2016-06-23 President And Fellows Of Harvard College Serum-free in vitro directed differentiation protocol for generating stem cell-derived b cells and uses thereof
CN107614678B (zh) 2014-12-18 2021-04-30 哈佛学院校长同事会 干细胞来源的β细胞的产生方法及其使用方法
JP6692834B2 (ja) 2015-02-09 2020-05-13 スリングショット バイオサイエンシーズ， インコーポレイテッド 調整可能な光学特性を有するヒドロゲル粒子およびその使用の方法
GB201603569D0 (en) 2016-03-01 2016-04-13 Koninklijke Nederlandse Akademie Van Wetenschappen Improved differentiation method
US10420803B2 (en) 2016-04-14 2019-09-24 Janssen Biotech, Inc. Differentiation of pluripotent stem cells to intestinal midgut endoderm cells
CA3042495A1 (en) 2016-11-10 2018-05-17 Viacyte, Inc. Pdx1 pancreatic endoderm cells in cell delivery devices and methods thereof
GB201700504D0 (en) * 2017-01-11 2017-02-22 Francis Crick Inst Ltd Composition
JP6913694B2 (ja) 2017-05-31 2021-08-04 プロモセル・ゲー・エム・ベー・ハー 多能性幹細胞のための培養培地
CN111565707A (zh) 2017-06-14 2020-08-21 森玛治疗公司 用于递送治疗物的装置和方法
US10391156B2 (en) 2017-07-12 2019-08-27 Viacyte, Inc. University donor cells and related methods
EP3710021A4 (en) 2017-11-15 2021-08-11 Semma Therapeutics, Inc. ISLAND CELL MANUFACTURING COMPOSITIONS AND METHOD OF USE
GB201721615D0 (en) 2017-12-21 2018-02-07 Koninklijke Nederlandse Akademie Van Wetenschappen Immune cell organoid co-cultures
WO2020033879A1 (en) 2018-08-10 2020-02-13 Semma Therapeutics, Inc. Stem cell derived islet differentiation
US12378572B2 (en) 2018-09-07 2025-08-05 Crispr Therapeutics Ag Universal donor cells
GB201819224D0 (en) 2018-11-26 2019-01-09 Koninklijke Nederlandse Akademie Van Wetenschappen Hepatocyte expansion methods
EP3975926A1 (en) 2019-05-31 2022-04-06 W.L. Gore & Associates, Inc. A biocompatible membrane composite
EP3976236A1 (en) 2019-05-31 2022-04-06 W.L. Gore & Associates Inc. A biocompatible membrane composite
CN114173837A (zh) 2019-05-31 2022-03-11 W.L.戈尔及同仁股份有限公司 生物相容性膜复合材料
CA3139292C (en) 2019-05-31 2025-06-10 Viacyte, Inc. CELL ENCAPSULATION DEVICES WITH REGULATED OXYGEN DIFFUSION DISTANCES
PH12022550542A1 (en) 2019-09-05 2023-03-20 Crispr Therapeutics Ag Universal donor cells
CA3150235A1 (en) 2019-09-05 2021-03-11 Alireza Rezania Universal donor cells
CN112779209B (zh) * 2019-11-08 2023-01-24 合肥中科普瑞昇生物医药科技有限公司 原代乳腺上皮细胞培养基、培养方法及其应用
KR102268242B1 (ko) * 2020-01-06 2021-06-23 에스씨엠생명과학 주식회사 줄기세포의 기능강화용 조성물
CN111492979B (zh) * 2020-05-25 2022-03-22 四川天艺生态园林集团股份有限公司 一种小报春体细胞胚诱导方法
US12577540B2 (en) 2020-12-10 2026-03-17 Sarepta Therapeutics, Inc. Suspension mode seed train development for adherent cells
WO2022144855A1 (en) 2020-12-31 2022-07-07 Crispr Therapeutics Ag Universal donor cells
US11735303B2 (en) 2021-06-22 2023-08-22 David Haase Apparatus and method for determining a composition of a replacement therapy treatment
JP7829851B2 (ja) * 2021-10-14 2026-03-16 大日本印刷株式会社 組織の製造方法
AU2022376541A1 (en) 2021-10-29 2024-04-18 Slingshot Biosciences, Inc. Hydrogel particles as feeder cells and as synthetic antigen presenting cells
CN120712108A (zh) 2022-10-26 2025-09-26 弹弓生物科学公司 具有可调整光学性质的大小可调整合成颗粒和用于使用其进行免疫细胞激活的方法
CN119192326B (zh) * 2024-10-23 2025-06-17 四川大学 重组cFGF2蛋白和鸟类原始生殖细胞培养基及其应用

Family Cites Families (62)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0505148A1 (en) 1991-03-17 1992-09-23 Yeda Research And Development Company Limited Stimulating factor of the neu receptor
WO1993022424A1 (en) 1992-04-29 1993-11-11 Amgen Inc. RECOMBINANT STIMULATING FACTOR OF THE neu RECEPTOR
US5453357A (en) 1992-10-08 1995-09-26 Vanderbilt University Pluripotential embryonic stem cells and methods of making same
WO1994028133A1 (en) 1993-05-21 1994-12-08 Amgen Inc. Recombinant neu differentiation factors
US5843780A (en) 1995-01-20 1998-12-01 Wisconsin Alumni Research Foundation Primate embryonic stem cells
US6225282B1 (en) 1996-01-05 2001-05-01 Genentech, Inc. Treatment of hearing impairments
JP2001517927A (ja) 1996-02-28 2001-10-09 バンダービルト ユニバーシティ 胚幹細胞を作製する組成物および方法
CA2258721C (en) * 1996-07-12 2014-09-09 Genentech, Inc. Chimeric heteromultimer adhesins
CA2277278A1 (en) * 1997-01-10 1998-07-16 Life Technologies, Inc. Embryonic stem cell serum replacement
US6387638B1 (en) 1997-02-10 2002-05-14 Genentech, Inc. Heregulin variants
US6136558A (en) 1997-02-10 2000-10-24 Genentech, Inc. Heregulin variants
US6331406B1 (en) 1997-03-31 2001-12-18 The John Hopkins University School Of Medicine Human enbryonic germ cell and methods of use
WO1998059035A2 (en) * 1997-06-25 1998-12-30 The Goverment Of The United States Of America, Represented By The Secretary, Dept. Of Health And Human Services, National Institutes Of Health Serum-free cell growth medium
DK1510579T3 (da) * 1998-02-04 2009-11-30 Genentech Inc Anvendelse af heregulin som en epitelcellevækstfaktor
CA2324591A1 (en) 1998-04-09 1999-10-21 Bresagen Limited Cell differentiation/proliferation and maintenance factor and uses thereof
US6667176B1 (en) 2000-01-11 2003-12-23 Geron Corporation cDNA libraries reflecting gene expression during growth and differentiation of human pluripotent stem cells
US6875607B1 (en) 1998-11-09 2005-04-05 Es Cell International Pte Ltd Embryonic stem cells
AU4209300A (en) * 1999-04-06 2000-10-23 Genentech Inc. Use of erbb receptor ligands in treating diabetes
CA2377906A1 (en) * 1999-07-16 2001-01-25 Prescient Neuropharma Inc. Methods for producing and preparing cells for cell therapy
US7439064B2 (en) * 2000-03-09 2008-10-21 Wicell Research Institute, Inc. Cultivation of human embryonic stem cells in the absence of feeder cells or without conditioned medium
JP2003531588A (ja) * 2000-04-11 2003-10-28 ジェネンテック・インコーポレーテッド 多価抗体とその用途
US20030096414A1 (en) * 2001-03-27 2003-05-22 Invitrogen Corporation Culture medium for cell growth and transfection
WO2003014313A2 (en) 2001-08-06 2003-02-20 Bresagen, Ltd. Alternative compositions and methods for the culture of stem cells
MXPA04005010A (es) * 2001-11-26 2005-04-08 Advanced Cell Tech Inc METODO PARA HACER Y USAR NUCLEOS CELULARES SOMáTICOS METODOS PARA HACER Y USAR NUCLEOS CELULARES SOMáTICOS HUMANOS REPROGRAMADOS Y CELULAS DEL TALLO HUMANAS AUTOLOGAS E ISOGENICAS.
EP1480520A4 (en) * 2002-02-12 2006-02-15 Raven Biotechnologies Inc EPITHELIAL CELLS FROM THE HUMAN FOLDS BUBBLE
EP1654377A4 (en) * 2002-03-01 2010-03-24 Roger Williams Hospital PROCESSES AND COMPOSITIONS RELATED TO THE SHC PROTEIN FOR THE PROGNOSIS OF BREAST, PROSTATE AND EGG CANCER
WO2004015077A2 (en) 2002-08-08 2004-02-19 University Of Georgia Research Foundation, Inc. Compositions and methods for neural differentiation of embryonic stem cells
JP2005537803A (ja) * 2002-09-06 2005-12-15 アムサイト インコーポレーティッド Ｃｄ５６陽性ヒト成体膵臓内分泌前駆細胞
WO2004029203A2 (en) 2002-09-25 2004-04-08 Bresagen, Inc. Compositions and methods for enrichment of neural stem cells using ceramide analogs
US20040121464A1 (en) * 2002-09-30 2004-06-24 Rathjen Peter David Method for the preparation of cells of mesodermal lineage
US20050031598A1 (en) * 2002-12-10 2005-02-10 Shulamit Levenberg Engineering three-dimensional tissue structures using differentiating embryonic stem cells
US20060211111A1 (en) 2002-12-18 2006-09-21 Maisam Mitalipova Compositions and methods for neural cell production and stabilization
US20060194315A1 (en) 2003-03-31 2006-08-31 Condie Brian G Compositions and methods for the control, differentiaton and/or manipulation of pluripotent cells through a gamma-secretase signaling pathway
KR20060017585A (ko) * 2003-04-04 2006-02-24 유니베르시떼 드 로잔느 암 치료용 펩타바디
US7820439B2 (en) * 2003-09-03 2010-10-26 Reliance Life Sciences Pvt Ltd. In vitro generation of GABAergic neurons from pluripotent stem cells
US20060030042A1 (en) 2003-12-19 2006-02-09 Ali Brivanlou Maintenance of embryonic stem cells by the GSK-3 inhibitor 6-bromoindirubin-3'-oxime
US7541185B2 (en) 2003-12-23 2009-06-02 Cythera, Inc. Methods for identifying factors for differentiating definitive endoderm
CN103898047B (zh) 2003-12-23 2020-03-03 维亚希特公司 定形内胚层
US8647873B2 (en) 2004-04-27 2014-02-11 Viacyte, Inc. PDX1 expressing endoderm
US8586357B2 (en) 2003-12-23 2013-11-19 Viacyte, Inc. Markers of definitive endoderm
US7985585B2 (en) 2004-07-09 2011-07-26 Viacyte, Inc. Preprimitive streak and mesendoderm cells
US20050266554A1 (en) 2004-04-27 2005-12-01 D Amour Kevin A PDX1 expressing endoderm
JP4657108B2 (ja) * 2003-12-26 2011-03-23 美保 古江 Ｅｓ細胞培養用基礎培地
WO2005065354A2 (en) 2003-12-31 2005-07-21 The Burnham Institute Defined media for pluripotent stem cell culture
EP2329833A1 (en) * 2004-01-27 2011-06-08 The Hospital for Sick Children Research Institute Methods of making and using skin-derived stem cells
JP4901471B2 (ja) * 2004-02-19 2012-03-21 国立大学法人京都大学 体細胞核初期化物質のスクリーニング方法
CN103103158B (zh) 2004-04-27 2016-08-03 韦尔赛特公司 细胞培养基
WO2006004149A1 (ja) * 2004-07-06 2006-01-12 Kyowa Hakko Kogyo Co., Ltd. 神経細胞の製造方法
CN102586170B (zh) 2004-07-09 2019-11-29 维亚希特公司 前原条和中内胚层细胞
AU2005272078B2 (en) 2004-07-09 2011-04-14 Viacyte, Inc. Methods for identifying factors for differentiating definitive endoderm
JP2006042663A (ja) * 2004-08-03 2006-02-16 Reprocell Inc Ｅｓ細胞の識別マーカー
US8187878B2 (en) 2004-08-13 2012-05-29 University Of Georgia Research Foundation, Inc. Methods for increasing definitive endoderm differentiation of pluripotent human embryonic stem cells with PI-3 kinase inhibitors
WO2007002210A2 (en) 2005-06-20 2007-01-04 Bresagen, Inc. Embryonic stem cell culture compositions and methods of use thereof
WO2007051038A2 (en) 2005-10-27 2007-05-03 Cythera, Inc. Pdx1-expressing dorsal and ventral foregut endoderm
US8278104B2 (en) 2005-12-13 2012-10-02 Kyoto University Induced pluripotent stem cells produced with Oct3/4, Klf4 and Sox2
SG170021A1 (en) 2006-02-23 2011-04-29 Novocell Inc Compositions and methods useful for culturing differentiable cells
JP6110048B2 (ja) 2006-03-02 2017-04-05 ヴィアサイト，インコーポレイテッド 内分泌前駆細胞、膵臓ホルモン発現細胞及びそれらの製造方法
US7737253B2 (en) 2006-06-16 2010-06-15 Bresagen, Inc. Human cancer stem cell culture compositions comprising Erbb2 variants and methods of use thereof
JP2010518459A (ja) 2007-02-14 2010-05-27 ミューズアミ， インコーポレイテッド 配布オーディオファイル編集用ウェブポータル
US7695963B2 (en) 2007-09-24 2010-04-13 Cythera, Inc. Methods for increasing definitive endoderm production
US8623650B2 (en) 2007-10-19 2014-01-07 Viacyte, Inc. Methods and compositions for feeder-free pluripotent stem cell media containing human serum
US20090298178A1 (en) 2008-06-03 2009-12-03 D Amour Kevin Allen Growth factors for production of definitive endoderm

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