DK1994171T3 - Multiplexanalyser af testprøver - Google Patents

Multiplexanalyser af testprøver Download PDF

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DK1994171T3
DK1994171T3 DK07718147.7T DK07718147T DK1994171T3 DK 1994171 T3 DK1994171 T3 DK 1994171T3 DK 07718147 T DK07718147 T DK 07718147T DK 1994171 T3 DK1994171 T3 DK 1994171T3
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aptamer
target
test sample
complex
target molecule
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DK07718147.7T
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James R Heil
Daniel J Schneider
Daniel T Nieuwlandt
Sheri K Wilcox
Dominic Zichi
Todd Gander
Bruce Eaton
Larry Gold
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Somalogic Inc
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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/5308—Immunoassay; Biospecific binding assay; Materials therefor for analytes not provided for elsewhere, e.g. nucleic acids, uric acid, worms, mites
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
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    • C12Q1/6832—Enhancement of hybridisation reaction
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
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    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/115—Aptamers, i.e. nucleic acids binding a target molecule specifically and with high affinity without hybridising therewith ; Nucleic acids binding to non-nucleic acids, e.g. aptamers
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    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/16—Aptamers
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    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/30—Chemical structure
    • C12N2310/35—Nature of the modification
    • C12N2310/351—Conjugate
    • C12N2310/3519—Fusion with another nucleic acid
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    • C12Q2523/00—Reactions characterised by treatment of reaction samples
    • C12Q2523/30—Characterised by physical treatment
    • C12Q2523/313—Irradiation, e.g. UV irradiation
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    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/161—Modifications characterised by incorporating target specific and non-target specific sites
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    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/205—Aptamer
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    • C12Q2537/00—Reactions characterised by the reaction format or use of a specific feature
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    • C12Q2537/101—Homogeneous assay format, e.g. one pot reaction
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    • C12Q2565/00—Nucleic acid analysis characterised by mode or means of detection
    • C12Q2565/50—Detection characterised by immobilisation to a surface
    • C12Q2565/514—Detection characterised by immobilisation to a surface characterised by the use of the arrayed oligonucleotides as identifier tags, e.g. universal addressable array, anti-tag or tag complement array

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  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Other Investigation Or Analysis Of Materials By Electrical Means (AREA)

Claims (15)

1
1. Fremgangsmåde til påvisning af et målmolekyle, som eventuelt er til stede i en testprøve, hvilken fremgangsmåde omfatter: 5 (a) at bringe en testprøve i kontakt med en aptamer med en specifik affinitet for målmolekylet, hvor et aptameraffinitetskompleks dannes ved en aptamers interaktion med dens målmolekyle, hvis målmolekylet er til stede i testprøven, hvor et aptameraffinitetskompleks er et ikke-kovalent kompleks dannet ved en aptamers interaktion med dens målmolekyle; 10 (b) efter dannelse af et aptameraffinitetskompleks i trin (a) at indføre et kon kurrentmolekyle i testprøven og/eller at indfange aptameraffinitetskomplekset på en fast bærer og efterfølgende at vaske med en opløsning med et konkurrentmolekyle, der er til stede i opløsningen, hvor konkurrentmolekylet er udvalgt blandt et oligonukleotid, en polyanion, en polydextran eller dNTPs; og 15 (c) at påvise og/eller kvantificere aptameren i aptameraffinitetskomplekset eller aptameraffinitetskomplekset, der er adskilt fra resten af testprøven.
2. Fremgangsmåde ifølge krav 1, hvor konkurrenten er en polyanion.
3. Fremgangsmåde ifølge krav 2, hvor polyanionen er dextransulfat.
4. Fremgangsmåde ifølge krav 2, hvor polyanionen er heparin eller polydextran.
5. Fremgangsmåde ifølge et af kravene 1 til 4, hvor aptameren er en enkelt- strenget nukleinsyre eller en dobbeltstrenget nukleinsyre.
6. Fremgangsmåde ifølge krav 5, hvor aptameren er DNA eller RNA.
7. Fremgangsmåde ifølge et af kravene 1 til 6, hvor målmolekylet er udvalgt fra gruppen bestående af et protein, et kulhydrat, en polysaccharid, et glycoprotein, et hormon, en receptor, et antigen, et antistof, en virus, et substrat, en metabolit, en cofaktor, en inhibitor, et lægemiddel, et farvestof, et næringsmiddel, en vækstfaktor og et væv. 35
8. Fremgangsmåde ifølge krav 7, hvor målmolekylet er et protein. 2
9. Fremgangsmåde ifølge krav 8, hvor proteinet er et glycoprotein.
10. Fremgangsmåde ifølge krav 8, hvor proteinet er en receptor eller et anti- 5 stof.
11. Fremgangsmåde ifølge krav 7, hvor målmolekylet er et kulhydrat.
12. Fremgangsmåde ifølge krav 11, hvor kulhydratet er en polysaccharid. 10
13. Fremgangsmåde ifølge et af kravene 1 til 12, hvor testprøven er en biologisk prøve fra gruppen bestående af helblod, leukocytter, perifere mononukleare blodceller, plasma, serum, spyt, ånde, urin, sæd, savl, hjernehindevæske, amniotisk væske, kirtelvæske, lymfevæske, brystvorteaspirat, bron- 15 kieaspirat, synovialvæske, ledaspirat, celler, et celleekstrakt, fæces, væv, en vævsbiopsi og cerebrospinalvæske.
14. Fremgangsmåde ifølge krav 13, hvor testprøven er en biologisk prøve af celler. 20
15. Fremgangsmåde ifølge krav 14, hvor cellerne er leukocytter eller perifere mononukleare blodceller.
DK07718147.7T 2006-01-17 2007-01-16 Multiplexanalyser af testprøver DK1994171T3 (da)

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US75967506P 2006-01-17 2006-01-17
PCT/US2007/060557 WO2007084886A2 (en) 2006-01-17 2007-01-16 Multiplexed analyses of test samples

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US (1) US20070166740A1 (da)
EP (3) EP2952894B1 (da)
JP (3) JP5680827B2 (da)
KR (1) KR101149688B1 (da)
CN (1) CN101374965B (da)
AU (1) AU2007205974A1 (da)
CA (1) CA2634987C (da)
DK (1) DK1994171T3 (da)
ES (3) ES2538121T3 (da)
HU (1) HUE025489T2 (da)
PL (1) PL1994171T3 (da)
PT (1) PT1994171E (da)
SI (1) SI1994171T1 (da)
TW (1) TWI507528B (da)
WO (1) WO2007084886A2 (da)

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