DK200400936A - Fremgangsmåde til fremstilling af L-tert.-leucin - Google Patents
Fremgangsmåde til fremstilling af L-tert.-leucin Download PDFInfo
- Publication number
- DK200400936A DK200400936A DK200400936A DKPA200400936A DK200400936A DK 200400936 A DK200400936 A DK 200400936A DK 200400936 A DK200400936 A DK 200400936A DK PA200400936 A DKPA200400936 A DK PA200400936A DK 200400936 A DK200400936 A DK 200400936A
- Authority
- DK
- Denmark
- Prior art keywords
- dsm
- transaminated
- leucine
- tert
- preparation
- Prior art date
Links
- 238000000034 method Methods 0.000 title description 12
- ROHFNLRQFUQHCH-YFKPBYRVSA-N L-leucine Chemical compound CC(C)C[C@H](N)C(O)=O ROHFNLRQFUQHCH-YFKPBYRVSA-N 0.000 title 1
- 125000003277 amino group Chemical group 0.000 description 3
- 244000005700 microbiome Species 0.000 description 3
- 241000588724 Escherichia coli Species 0.000 description 2
- 241000589516 Pseudomonas Species 0.000 description 2
- 150000004716 alpha keto acids Chemical class 0.000 description 2
- 238000005891 transamination reaction Methods 0.000 description 2
- IAWVHZJZHDSEOC-UHFFFAOYSA-N 3,3-dimethyl-2-oxobutanoic acid Chemical compound CC(C)(C)C(=O)C(O)=O IAWVHZJZHDSEOC-UHFFFAOYSA-N 0.000 description 1
- 241001453369 Achromobacter denitrificans Species 0.000 description 1
- 241000589158 Agrobacterium Species 0.000 description 1
- 241000589155 Agrobacterium tumefaciens Species 0.000 description 1
- 241000588813 Alcaligenes faecalis Species 0.000 description 1
- 241000588914 Enterobacter Species 0.000 description 1
- 241000305071 Enterobacterales Species 0.000 description 1
- 241001452028 Escherichia coli DH1 Species 0.000 description 1
- 241000588912 Pantoea agglomerans Species 0.000 description 1
- 241001057811 Paracoccus <mealybug> Species 0.000 description 1
- 241000589597 Paracoccus denitrificans Species 0.000 description 1
- 241000607720 Serratia Species 0.000 description 1
- 241001622809 Serratia plymuthica Species 0.000 description 1
- 241000736110 Sphingomonas paucimobilis Species 0.000 description 1
- 241000187747 Streptomyces Species 0.000 description 1
- 241000187391 Streptomyces hygroscopicus Species 0.000 description 1
- 241000187191 Streptomyces viridochromogenes Species 0.000 description 1
- 102000003929 Transaminases Human genes 0.000 description 1
- 108090000340 Transaminases Proteins 0.000 description 1
- 229940005347 alcaligenes faecalis Drugs 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 239000000284 extract Substances 0.000 description 1
- 101150099953 ilvE gene Proteins 0.000 description 1
- 239000013612 plasmid Substances 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 239000002689 soil Substances 0.000 description 1
- 101150028338 tyrB gene Proteins 0.000 description 1
Landscapes
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Enzymes And Modification Thereof (AREA)
Description
PATENTKRAV 1. Fremgangsmåde til fremstilling af L-tert.-leucin kendetegnet ved, at 3,3-dimethyl-2-oxo-butansyre eller et salt af denne forbindelse, hver gang i nærværelse af aminosyrer som aminogruppedonatorer, transamineres. 2. Fremgangsmåde ifølge krav 1, kendetegnet ved, at der transamineres ved hjælp af mikroorganismer . 3. Fremgangsmåde ifølge krav 2, kendetegnet ved, at der transamineres ved hjælp af mikroorganismer af slægterne Paracoccus, Alkaligenes, Pseudomonas, Serratia, Agrobacterium og Streptomyces samt enterobakterier. 4. Fremgangsmåde ifølge krav 1 og 2, kendetegnet ved, at der transamineres ved hjælp af Alcaligenes faecalis DSM 4115, Alcaligenes denitrificans DSM 4114, Pseudomonas paucimobilis DSM 4120, Pseudomonas spec. 4119, Serratia plymuthica DSM 4116, Agrobacterium tumefaciens, Escherichia coli DHl, Escherichia coli ATCC 11303, Entero-bacter agglomerans DSM 4112, Enterobacter spec. DSM 4121, Paracoccus denitrificans DSM 65, Streptomyces hygroscopicus og Streptomyces viridochromogenes samt 3 jordisolater DSM 4113, DSM 4117 og DSM 4118. 5. Fremgangsmåde ifølge krav 2, kendetegne t ved, at der transamineres ved hjælp af genteknisk manipulerede mikroorganismer. 6. Fremgangsmåde ifølge krav 5, kendetegnet ved, at der transamineres ved hjælp af E. coli ATCC 11303, som er transformeret med et plasmid indeholdende et tyrB-gen eller et ilvE-gen. 7. Fremgangsmåde ifølge et eller flere af kravene 1-6, kendetegnet ved, at der transamineres med celleekstrakter, isolerede hele proteiner eller med rensede transaminaser. 8. Fremgangsmåde ifølge et eller flere af kravene 1-7, kendetegnet ved, at aminogruppedonator og α-ketosyre anvendes i et forhold fra 1:1 til 5:1. 9. Fremgangsmåde ifølge krav 8, kendetegnet ved, at aminogruppedonator og α-ketosyre anvendes i et forhold fra 1:1 til 2:1. 10. Fremgangsmåde ifølge et eller flere af kravene 1-9, kendetegnet ved, at transamineringen gennemføres i et pH-værdiområde fra 5 til 9. 11. Fremgangsmåde ifølge krav 10, kendetegnet ved, at transmineringen gennemføres i et pH-værdiom-råde fra 7 til 8,5.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DK200400936A DK175472B1 (da) | 1986-06-04 | 2004-06-16 | Fremgangsmåde til fremstilling af L-tert.-leucin |
Applications Claiming Priority (6)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DE3618812 | 1986-06-04 | ||
| DE3618812 | 1986-06-04 | ||
| DK198702848A DK175474B1 (da) | 1986-06-04 | 1987-06-03 | Fremgangsmåde til fremstilling af L-phosphinothricin samt ved fremgangsmåden anvendelige mikroorganismer |
| DK284887 | 1987-06-03 | ||
| DK200400936 | 2004-06-16 | ||
| DK200400936A DK175472B1 (da) | 1986-06-04 | 2004-06-16 | Fremgangsmåde til fremstilling af L-tert.-leucin |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| DK200400936A true DK200400936A (da) | 2004-06-16 |
| DK175472B1 DK175472B1 (da) | 2004-11-08 |
Family
ID=32683313
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DK200400936A DK175472B1 (da) | 1986-06-04 | 2004-06-16 | Fremgangsmåde til fremstilling af L-tert.-leucin |
Country Status (1)
| Country | Link |
|---|---|
| DK (1) | DK175472B1 (da) |
-
2004
- 2004-06-16 DK DK200400936A patent/DK175472B1/da not_active IP Right Cessation
Also Published As
| Publication number | Publication date |
|---|---|
| DK175472B1 (da) | 2004-11-08 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PUP | Patent expired |