DK200401111A - Poly:peptide with granulocyte colony stimulating factor activity - obtd. by recombinant DNA procedures for treating haematopoietic disorders - Google Patents
Poly:peptide with granulocyte colony stimulating factor activity - obtd. by recombinant DNA procedures for treating haematopoietic disorders Download PDFInfo
- Publication number
- DK200401111A DK200401111A DK200401111A DKPA200401111A DK200401111A DK 200401111 A DK200401111 A DK 200401111A DK 200401111 A DK200401111 A DK 200401111A DK PA200401111 A DKPA200401111 A DK PA200401111A DK 200401111 A DK200401111 A DK 200401111A
- Authority
- DK
- Denmark
- Prior art keywords
- stimulating factor
- colony stimulating
- granulocyte colony
- obtd
- peptide
- Prior art date
Links
- 102000004269 Granulocyte Colony-Stimulating Factor Human genes 0.000 title abstract description 3
- 108010017080 Granulocyte Colony-Stimulating Factor Proteins 0.000 title abstract description 3
- 108090000765 processed proteins & peptides Proteins 0.000 title abstract description 3
- 238000000034 method Methods 0.000 title description 6
- 108020004511 Recombinant DNA Proteins 0.000 title 1
- 230000000694 effects Effects 0.000 title 1
- 230000003394 haemopoietic effect Effects 0.000 title 1
- 210000004027 cell Anatomy 0.000 abstract description 6
- 230000004071 biological effect Effects 0.000 abstract description 2
- 229920001184 polypeptide Polymers 0.000 abstract description 2
- 102000004196 processed proteins & peptides Human genes 0.000 abstract description 2
- 108091028043 Nucleic acid sequence Proteins 0.000 abstract 2
- 108091029865 Exogenous DNA Proteins 0.000 abstract 1
- 230000000295 complement effect Effects 0.000 abstract 1
- 239000012634 fragment Substances 0.000 abstract 1
- 230000002068 genetic effect Effects 0.000 abstract 1
- 241000288906 Primates Species 0.000 description 2
- 125000003275 alpha amino acid group Chemical group 0.000 description 1
- 230000001377 granulocytopoietic effect Effects 0.000 description 1
- 238000001727 in vivo Methods 0.000 description 1
- 210000004962 mammalian cell Anatomy 0.000 description 1
- 230000003612 virological effect Effects 0.000 description 1
Landscapes
- Peptides Or Proteins (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Abstract
Purified and isolated polypeptide (I) having at least part of the primary structural conformation and a biological property (ies) of naturally occurring pluripotent granulocyte colony stimulating factor (hpG-CSF) is new when the (I) is the prod. of procaryotic or eucaryotic expression of an exogenous DNA sequence. - DNA sequence for use in securing expression of (I) in a procaryotic or eucaryotic host cell is new. The DNA sequence is either a defined sequence of its complementary strands, or a sequence that hybridises to such sequences or their fragments, or a sequence that, but for the degeneracy of the genetic code, would hydridise to any of these other sequences.
Description
PATENTKRAV 1. Fremgangsmåde til fremstilling af et humant pluripotent granulocyt kolonistimulerende faktor{hpG-CSF)produkt med in vivo granulocytopoietiske, biologiske egenskaber som naturligt forekommende hpG-CSF, kendetegnet ved, at fremgangsmåden omfatter følgende trin: (a) under hensigtsmæssige næringsbetingelser at dyrke pattedyrsceller omfattende andet promotor DNA end hpG-CSF promotor DNA og operativt forbundet med DNA kodende for et hpg-CSF polypeptid med den modne aminosyresekvens ifølge figur Vil; og (b) isolere det af cellerne eksprimerede hpg-CSF produkt . 2. Fremgangsmåde ifølge krav 1, kende tegnet ved, at promotor DNA er viral promotor DNA. 3. Fremgangsmåde ifølge krav 1, kende tegnet ved, at cellerne er primat-celler. 4. Fremgangsmåde ifølge krav 3, kende tegnet ved, at primat-cellerne er COS celler.A process for the preparation of a human pluripotent granulocyte colony-stimulating factor (hpG-CSF) product with in vivo granulocytopoietic biological properties as naturally occurring hpG-CSF, characterized in that the method comprises the following steps: (a) mammalian cells comprising promoter DNA other than hpG-CSF promoter DNA and operably linked to DNA encoding a hpg-CSF polypeptide with the mature amino acid sequence of Figure Vil; and (b) isolating the hpg-CSF product expressed by the cells. The method of claim 1, characterized in that the promoter DNA is viral promoter DNA. The method of claim 1, characterized in that the cells are primate cells. The method of claim 3, characterized in that the primate cells are COS cells.
Applications Claiming Priority (6)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US76895985A | 1985-08-23 | 1985-08-23 | |
| US76895985 | 1985-08-23 | ||
| US83554886 | 1986-03-03 | ||
| US06/835,548 US4810643A (en) | 1985-08-23 | 1986-03-03 | Production of pluripotent granulocyte colony-stimulating factor |
| DK200301685 | 2003-11-12 | ||
| DK200301685A DK175551B1 (en) | 1985-08-23 | 2003-11-12 | Poly:peptide with granulocyte colony stimulating factor activity - obtd. by recombinant DNA procedures for treating haematopoietic disorders |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| DK200401111A true DK200401111A (en) | 2004-07-15 |
| DK176002B1 DK176002B1 (en) | 2005-11-21 |
Family
ID=32872384
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DK200401111A DK176002B1 (en) | 1985-08-23 | 2004-07-15 | Poly:peptide with granulocyte colony stimulating factor activity - obtd. by recombinant DNA procedures for treating haematopoietic disorders |
Country Status (1)
| Country | Link |
|---|---|
| DK (1) | DK176002B1 (en) |
-
2004
- 2004-07-15 DK DK200401111A patent/DK176002B1/en not_active IP Right Cessation
Also Published As
| Publication number | Publication date |
|---|---|
| DK176002B1 (en) | 2005-11-21 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PUP | Patent expired |