DK2004827T3 - Forbedret fremgangsmåde til homolog rekombination i svampeceller - Google Patents

Forbedret fremgangsmåde til homolog rekombination i svampeceller

Info

Publication number
DK2004827T3
DK2004827T3 DK07726886.0T DK07726886T DK2004827T3 DK 2004827 T3 DK2004827 T3 DK 2004827T3 DK 07726886 T DK07726886 T DK 07726886T DK 2004827 T3 DK2004827 T3 DK 2004827T3
Authority
DK
Denmark
Prior art keywords
homologous recombination
improved method
fungal cells
sequence
disclosed
Prior art date
Application number
DK07726886.0T
Other languages
English (en)
Inventor
De Paulo Boer
Van Den Marco Alexander Berg
Remko Offringa
Original Assignee
Dsm Ip Assets Bv
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Dsm Ip Assets Bv filed Critical Dsm Ip Assets Bv
Application granted granted Critical
Publication of DK2004827T3 publication Critical patent/DK2004827T3/da

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C12N15/815—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts for yeasts other than Saccharomyces
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
    • C12N15/905—Stable introduction of foreign DNA into chromosome using homologous recombination in yeast
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2800/00—Nucleic acids vectors
    • C12N2800/30—Vector systems comprising sequences for excision in presence of a recombinase, e.g. loxP or FRT
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2800/00—Nucleic acids vectors
    • C12N2800/70—Vectors containing special elements for cloning, e.g. topoisomerase, adaptor sites

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Mycology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Bipolar Transistors (AREA)
DK07726886.0T 2006-04-08 2007-03-14 Forbedret fremgangsmåde til homolog rekombination i svampeceller DK2004827T3 (da)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP06075904 2006-04-08
PCT/EP2007/052393 WO2007115887A1 (en) 2006-04-08 2007-03-14 Improved method for homologous recombination in fungal cells

Publications (1)

Publication Number Publication Date
DK2004827T3 true DK2004827T3 (da) 2010-06-14

Family

ID=38267581

Family Applications (2)

Application Number Title Priority Date Filing Date
DK07726885.2T DK2004826T3 (da) 2006-04-08 2007-03-14 Forbedret fremgangsmåde til homolog rekombination i eukaryotiske celler
DK07726886.0T DK2004827T3 (da) 2006-04-08 2007-03-14 Forbedret fremgangsmåde til homolog rekombination i svampeceller

Family Applications Before (1)

Application Number Title Priority Date Filing Date
DK07726885.2T DK2004826T3 (da) 2006-04-08 2007-03-14 Forbedret fremgangsmåde til homolog rekombination i eukaryotiske celler

Country Status (7)

Country Link
US (3) US20090124014A1 (da)
EP (2) EP2004826B1 (da)
CN (1) CN101421401A (da)
AT (2) ATE460489T1 (da)
DE (2) DE602007012846D1 (da)
DK (2) DK2004826T3 (da)
WO (2) WO2007115886A1 (da)

Families Citing this family (27)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7888121B2 (en) 2003-08-08 2011-02-15 Sangamo Biosciences, Inc. Methods and compositions for targeted cleavage and recombination
US11311574B2 (en) 2003-08-08 2022-04-26 Sangamo Therapeutics, Inc. Methods and compositions for targeted cleavage and recombination
CA2559827A1 (en) 2004-04-02 2005-10-13 Dsm Ip Assets B.V. Filamentous fungal mutants with improved homologous recombination efficiency
EP2004826B1 (en) * 2006-04-08 2011-03-02 DSM IP Assets B.V. Improved method for homologous recombination in eukaryotic cells
EP2592149A1 (en) * 2007-03-21 2013-05-15 DSM IP Assets B.V. Improved method for homologous recombination
EP2123772A1 (en) 2008-04-29 2009-11-25 DSM IP Assets B.V. Beta-lactam antibiotic producing strains
EP2392649A3 (en) 2008-08-05 2012-01-11 DSM IP Assets B.V. Adipoyl-7-ADCA producing strains
WO2010122141A1 (en) 2009-04-24 2010-10-28 Dsm Ip Assets B.V. Carbohydrate degrading polypeptide and uses thereof
WO2011009700A1 (en) 2009-07-22 2011-01-27 Dsm Ip Assets B.V. Improved host cell for the production of a compound of interest
WO2011054899A1 (en) 2009-11-04 2011-05-12 Dsm Ip Assets B.V. Talaromyces transformants
AU2011214327B2 (en) 2010-02-11 2014-09-18 Dsm Ip Assets B.V. Polypeptide having cellobiohydrolase activity and uses thereof
CN102939382B (zh) * 2010-06-03 2017-02-15 丹尼斯科美国公司 丝状真菌宿主菌株和dna构建体以及它们的使用方法
US9163223B2 (en) 2010-06-29 2015-10-20 Dsm Ip Assets B.V. Polypeptide having acetyl xylan esterase activity and uses thereof
PL2588492T3 (pl) 2010-06-29 2016-09-30 Polipeptyd mający aktywność beta-glukozydazy i jego zastosowania
CA2803982A1 (en) 2010-06-29 2012-01-05 Dsm Ip Assets B.V. Polypeptide having beta-glucosidase activity and uses thereof
EP2588603B1 (en) 2010-06-29 2017-03-22 DSM IP Assets B.V. Polypeptide having or assisting in carbohydrate material degrading activity and uses thereof
WO2012000891A1 (en) 2010-06-29 2012-01-05 Dsm Ip Assets B.V. Polypeptide having carbohydrate degrading activity and uses thereof
AU2011273685B2 (en) 2010-06-29 2014-12-11 Dsm Ip Assets B.V. Polypeptide having swollenin activity and uses thereof
US9909152B2 (en) * 2011-11-30 2018-03-06 Battelle Memorial Institute Enhanced itaconic acid production in Aspergillus with increased LaeA expression
WO2014049168A1 (en) * 2012-09-28 2014-04-03 Ucb Pharma S.A. Methods for producing recombinant proteins
EP3686280B1 (en) 2013-02-04 2022-06-22 DSM IP Assets B.V. Carbohydrate degrading polypeptide and uses thereof
WO2014202624A2 (en) 2013-06-19 2014-12-24 Dsm Ip Assets B.V. Rasamsonia gene and use thereof
WO2014202622A2 (en) 2013-06-19 2014-12-24 Dsm Ip Assets B.V. Rasamsonia gene and use thereof
WO2014202620A2 (en) 2013-06-19 2014-12-24 Dsm Ip Assets B.V. Rasamsonia gene and use thereof
WO2014202621A1 (en) 2013-06-20 2014-12-24 Dsm Ip Assets B.V. Carbohydrate degrading polypeptide and uses thereof
CN104894165B (zh) * 2015-05-26 2019-01-25 中国科学院青岛生物能源与过程研究所 一种提高基因打靶技术在土曲霉中应用效率的方法与应用
CN104894115B (zh) * 2015-05-26 2018-11-13 中国科学院青岛生物能源与过程研究所 一种具有高效同源重组能力的土曲霉及其构建方法与应用

Family Cites Families (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5464764A (en) * 1989-08-22 1995-11-07 University Of Utah Research Foundation Positive-negative selection methods and vectors
WO1997006261A2 (en) * 1995-08-03 1997-02-20 Gist-Brocades B.V. THE USE OF HOMOLOGOUS amdS GENES AS SELECTABLE MARKERS
WO1999053017A2 (en) * 1998-04-15 1999-10-21 Fred Hutchinson Cancer Research Center Methods and vector constructs for making transgenic non-human animals which ubiquitously express a heterologous gene
WO2001042442A2 (en) * 1999-12-10 2001-06-14 Cytos Biotechnology Ag Activation of endogenous genes by genomic introduction of a replicon
EP1217074A1 (en) * 2000-12-22 2002-06-26 Universiteit Leiden Nucleic acid integration in eukaryotes
DE10131786A1 (de) * 2001-07-04 2003-01-16 Sungene Gmbh & Co Kgaa Rekombinationssysteme und Verfahren zum Entfernen von Nukleinsäuresequenzen aus dem Genom eukaryotischer Organismen
US20030092183A1 (en) * 2001-09-21 2003-05-15 Fisher Katherine E. Rapid creation of gene targeting vectors using homologous recombination in yeast
US8034790B2 (en) * 2003-12-01 2011-10-11 Introgen Therapeutics Use of MDA-7 to inhibit pathogenic infectious organisms
US20050181509A1 (en) * 2004-02-12 2005-08-18 The Penn State Research Foundation Dual selection based, targeted gene disruption method for fungi and fungus-like organisms
JP4050712B2 (ja) * 2004-02-27 2008-02-20 独立行政法人科学技術振興機構 相同組換えを行わせる方法
US20080134351A1 (en) * 2004-09-23 2008-06-05 Basf Plant Science Gmbh Recombination Cassettes and Methods For Sequence Excision in Plants
US20070155014A1 (en) * 2005-07-20 2007-07-05 Invitrogen Corporation Methods for increasing efficiency of homologous recombination
EP2004826B1 (en) * 2006-04-08 2011-03-02 DSM IP Assets B.V. Improved method for homologous recombination in eukaryotic cells
US8597923B2 (en) * 2009-05-06 2013-12-03 SyntheZyme, LLC Oxidation of compounds using genetically modified Candida

Also Published As

Publication number Publication date
WO2007115886A1 (en) 2007-10-18
DE602007005237D1 (de) 2010-04-22
ATE500333T1 (de) 2011-03-15
US20090170206A1 (en) 2009-07-02
ATE460489T1 (de) 2010-03-15
US20120282698A1 (en) 2012-11-08
EP2004827B1 (en) 2010-03-10
WO2007115887A1 (en) 2007-10-18
DE602007012846D1 (de) 2011-04-14
CN101421401A (zh) 2009-04-29
EP2004827A1 (en) 2008-12-24
EP2004826A1 (en) 2008-12-24
DK2004826T3 (da) 2011-05-16
EP2004826B1 (en) 2011-03-02
US20090124014A1 (en) 2009-05-14

Similar Documents

Publication Publication Date Title
DK2004826T3 (da) Forbedret fremgangsmåde til homolog rekombination i eukaryotiske celler
IN2014DN09261A (da)
ZA201606933B (en) Methods and compositions for increasing efficiency of targeted gene modification using oligonucleotide-mediated gene repair
AR084774A1 (es) Moleculas de acido nucleico que confieren resistencia a plagas coleopteros
MY183741A (en) Methods and compositions for increasing efficiency of targeted gene modification using oligonucleotide-mediated gene repair
WO2012112730A3 (en) Compositions and methods for delivering nucleic acid to a cell
IN2012DN02333A (da)
MX2013012904A (es) Microorganismos geneticamente modificados que metabolizan xilosa.
WO2010128024A3 (en) Reversibly immortalized cells as well as methods relating hereto
MY194500A (en) Methods and Compositions for Producing Fatty Alcohols
WO2011059920A3 (en) Methods of generating neural stem cells
WO2010078952A3 (de) Strukturierte gasdiffusionselektrode für elektrolysezellen
NZ700181A (en) Plant cytochrome p450
IT1398132B1 (it) Base per supportare piastrelle da tagliare, kit e metodo per adattare una macchina taglia piastrelle manuale.
PH12013502183A1 (en) Method for supplying sequential power impulses
DK1851815T3 (da) Fremgangsmåde til fremstilling af en brændselscellekatode
DK2126100T3 (da) Forbedret fremgangsmåde til homolog rekombination
CL2008002552A1 (es) Método para incorporar al menos dos factores genéticos en una planta de cultivo.
MY191390A (en) Methods and compositions for increasing efficiency of increased efficiency of targeted gene modification using oligonucleotide-mediated gene repair
DK2147133T3 (da) Elektrode til membranelektrolyseceller
EA201391684A1 (ru) Электрическое восстановление анода твердого оксидного топливного элемента
WO2011163328A8 (en) Methods for producing tissue scaffold directing differentiation of seeded cells and tissue scaffolds produced thereby
IL207749A0 (en) Methods to inhibit tumor cell growth by using proton pump inhibitors
MX361964B (es) Inhibición del crecimiento microbiano por inhibición de aconitasa.
MX2015006747A (es) Procedimiento para combatir plantas dañinas resistentes.