ATE500333T1 - Verbessertes verfahren zur homologen rekombination in eukaryontischen zellen - Google Patents

Verbessertes verfahren zur homologen rekombination in eukaryontischen zellen

Info

Publication number
ATE500333T1
ATE500333T1 AT07726885T AT07726885T ATE500333T1 AT E500333 T1 ATE500333 T1 AT E500333T1 AT 07726885 T AT07726885 T AT 07726885T AT 07726885 T AT07726885 T AT 07726885T AT E500333 T1 ATE500333 T1 AT E500333T1
Authority
AT
Austria
Prior art keywords
improved method
cells
eukaryontic
homologue recombination
homologue
Prior art date
Application number
AT07726885T
Other languages
English (en)
Inventor
Van Den Berg
Richard Kerkman
Hesselien Touw-Riel
Original Assignee
Dsm Ip Assets Bv
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Dsm Ip Assets Bv filed Critical Dsm Ip Assets Bv
Application granted granted Critical
Publication of ATE500333T1 publication Critical patent/ATE500333T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C12N15/815—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts for yeasts other than Saccharomyces
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
    • C12N15/905—Stable introduction of foreign DNA into chromosome using homologous recombination in yeast
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2800/00—Nucleic acids vectors
    • C12N2800/30—Vector systems comprising sequences for excision in presence of a recombinase, e.g. loxP or FRT
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2800/00—Nucleic acids vectors
    • C12N2800/70—Vectors containing special elements for cloning, e.g. topoisomerase, adaptor sites

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Mycology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Bipolar Transistors (AREA)
AT07726885T 2006-04-08 2007-03-14 Verbessertes verfahren zur homologen rekombination in eukaryontischen zellen ATE500333T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP06075904 2006-04-08
PCT/EP2007/052392 WO2007115886A1 (en) 2006-04-08 2007-03-14 Improved method for homologous recombination in eukaryotic cells

Publications (1)

Publication Number Publication Date
ATE500333T1 true ATE500333T1 (de) 2011-03-15

Family

ID=38267581

Family Applications (2)

Application Number Title Priority Date Filing Date
AT07726886T ATE460489T1 (de) 2006-04-08 2007-03-14 Verbessertes verfahren zur homologen rekombination in pilzzellen
AT07726885T ATE500333T1 (de) 2006-04-08 2007-03-14 Verbessertes verfahren zur homologen rekombination in eukaryontischen zellen

Family Applications Before (1)

Application Number Title Priority Date Filing Date
AT07726886T ATE460489T1 (de) 2006-04-08 2007-03-14 Verbessertes verfahren zur homologen rekombination in pilzzellen

Country Status (7)

Country Link
US (3) US20090124014A1 (de)
EP (2) EP2004826B1 (de)
CN (1) CN101421401A (de)
AT (2) ATE460489T1 (de)
DE (2) DE602007005237D1 (de)
DK (2) DK2004827T3 (de)
WO (2) WO2007115886A1 (de)

Families Citing this family (27)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US11311574B2 (en) 2003-08-08 2022-04-26 Sangamo Therapeutics, Inc. Methods and compositions for targeted cleavage and recombination
US7888121B2 (en) 2003-08-08 2011-02-15 Sangamo Biosciences, Inc. Methods and compositions for targeted cleavage and recombination
WO2005095624A2 (en) 2004-04-02 2005-10-13 Dsm Ip Assets B.V. Filamentous fungal mutants with improved homologous recombination efficiency
ATE460489T1 (de) * 2006-04-08 2010-03-15 Dsm Ip Assets Bv Verbessertes verfahren zur homologen rekombination in pilzzellen
DK2126100T3 (da) * 2007-03-21 2013-01-14 Dsm Ip Assets Bv Forbedret fremgangsmåde til homolog rekombination
EP2123772A1 (de) 2008-04-29 2009-11-25 DSM IP Assets B.V. Beta-Lactamantibiotikum produzierende Stämme
CN102131921A (zh) 2008-08-05 2011-07-20 帝斯曼知识产权资产管理有限公司 生产己二酰基-7-adca的菌株
BRPI1015536A2 (pt) 2009-04-24 2019-07-02 Dsm Ip Assets Bv polipeptídeo de degradação de carboidratos e usos do mesmo
EP3293264A1 (de) 2009-07-22 2018-03-14 DSM IP Assets B.V. Verbesserte wirtszelle für die herstellung von relevanten produkten
CA2779796A1 (en) 2009-11-04 2011-05-12 Dsm Ip Assets B.V. Talaromyces transformants
US9133448B2 (en) 2010-02-11 2015-09-15 Dsm Ip Assets B.V. Polypeptide having cellobiohydrolase activity and uses thereof
CA2801799C (en) * 2010-06-03 2018-11-20 Danisco Us Inc. Filamentous fungal host strains and dna constructs, and methods of use thereof
DK2588494T3 (en) 2010-06-29 2018-06-14 Dsm Ip Assets Bv POLYPEPTIDE WITH BETA-GLUCOSIDASE ACTIVITY AND APPLICATIONS THEREOF
BR112012033404B1 (pt) 2010-06-29 2021-05-18 Dsm Ip Assets B.V. célula recombinante, método para a preparação de um polipeptídeo, composição, método para o tratamento de um substrato que compreende material de carboidrato e uso de um polipeptídeo
CA2803513A1 (en) 2010-06-29 2012-01-05 Margot Elisabeth Francoise Schooneveld-Bergmans Polypeptide having acetyl xylan esterase activity and uses thereof
US9175050B2 (en) 2010-06-29 2015-11-03 Dsm Ip Assets B.V. Polypeptide having swollenin activity and uses thereof
CA2802128A1 (en) 2010-06-29 2012-01-05 Margot Elisabeth Francoise Schooneveld-Bergmans Polypeptide having beta-glucosidase activity and uses thereof
WO2012000891A1 (en) 2010-06-29 2012-01-05 Dsm Ip Assets B.V. Polypeptide having carbohydrate degrading activity and uses thereof
US9909152B2 (en) * 2011-11-30 2018-03-06 Battelle Memorial Institute Enhanced itaconic acid production in Aspergillus with increased LaeA expression
EP2900824A1 (de) * 2012-09-28 2015-08-05 UCB Biopharma SPRL Verfahren zur herstellung rekombinanter proteine
CN105073986A (zh) 2013-02-04 2015-11-18 帝斯曼知识产权资产管理有限公司 碳水化合物降解多肽及其用途
WO2014202620A2 (en) 2013-06-19 2014-12-24 Dsm Ip Assets B.V. Rasamsonia gene and use thereof
WO2014202622A2 (en) 2013-06-19 2014-12-24 Dsm Ip Assets B.V. Rasamsonia gene and use thereof
WO2014202624A2 (en) 2013-06-19 2014-12-24 Dsm Ip Assets B.V. Rasamsonia gene and use thereof
WO2014202621A1 (en) 2013-06-20 2014-12-24 Dsm Ip Assets B.V. Carbohydrate degrading polypeptide and uses thereof
CN104894115B (zh) * 2015-05-26 2018-11-13 中国科学院青岛生物能源与过程研究所 一种具有高效同源重组能力的土曲霉及其构建方法与应用
CN104894165B (zh) * 2015-05-26 2019-01-25 中国科学院青岛生物能源与过程研究所 一种提高基因打靶技术在土曲霉中应用效率的方法与应用

Family Cites Families (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5464764A (en) * 1989-08-22 1995-11-07 University Of Utah Research Foundation Positive-negative selection methods and vectors
ATE418614T1 (de) * 1995-08-03 2009-01-15 Dsm Ip Assets Bv Amds-gen aus aspergillus niger für eine acetamidase kodierend
DE69940196D1 (de) * 1998-04-15 2009-02-12 Hutchinson Fred Cancer Res Methoden und vektorenkonstrukte zur erzeugung von transgene nagetieren, welche ein heterologes gen ubiquitär exprimieren
AU1647501A (en) * 1999-12-10 2001-06-18 Cytos Biotechnology Ag Replicon based activation of endogenous genes
EP1217074A1 (de) * 2000-12-22 2002-06-26 Universiteit Leiden Integration von Nukleinsäure in Eukaryoten
DE10131786A1 (de) * 2001-07-04 2003-01-16 Sungene Gmbh & Co Kgaa Rekombinationssysteme und Verfahren zum Entfernen von Nukleinsäuresequenzen aus dem Genom eukaryotischer Organismen
US20030092183A1 (en) * 2001-09-21 2003-05-15 Fisher Katherine E. Rapid creation of gene targeting vectors using homologous recombination in yeast
US8034790B2 (en) * 2003-12-01 2011-10-11 Introgen Therapeutics Use of MDA-7 to inhibit pathogenic infectious organisms
US20050181509A1 (en) * 2004-02-12 2005-08-18 The Penn State Research Foundation Dual selection based, targeted gene disruption method for fungi and fungus-like organisms
JP4050712B2 (ja) * 2004-02-27 2008-02-20 独立行政法人科学技術振興機構 相同組換えを行わせる方法
US20080134351A1 (en) * 2004-09-23 2008-06-05 Basf Plant Science Gmbh Recombination Cassettes and Methods For Sequence Excision in Plants
US20070155014A1 (en) * 2005-07-20 2007-07-05 Invitrogen Corporation Methods for increasing efficiency of homologous recombination
ATE460489T1 (de) * 2006-04-08 2010-03-15 Dsm Ip Assets Bv Verbessertes verfahren zur homologen rekombination in pilzzellen
US8597923B2 (en) * 2009-05-06 2013-12-03 SyntheZyme, LLC Oxidation of compounds using genetically modified Candida

Also Published As

Publication number Publication date
CN101421401A (zh) 2009-04-29
WO2007115887A1 (en) 2007-10-18
DK2004826T3 (da) 2011-05-16
DK2004827T3 (da) 2010-06-14
EP2004826A1 (de) 2008-12-24
DE602007012846D1 (de) 2011-04-14
US20090170206A1 (en) 2009-07-02
WO2007115886A1 (en) 2007-10-18
EP2004827A1 (de) 2008-12-24
ATE460489T1 (de) 2010-03-15
US20090124014A1 (en) 2009-05-14
DE602007005237D1 (de) 2010-04-22
EP2004827B1 (de) 2010-03-10
EP2004826B1 (de) 2011-03-02
US20120282698A1 (en) 2012-11-08

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