DK2128248T4 - Oligonukleotidsammensætninger med forøget effektivitet - Google Patents

Oligonukleotidsammensætninger med forøget effektivitet Download PDF

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DK2128248T4
DK2128248T4 DK09166406.0T DK09166406T DK2128248T4 DK 2128248 T4 DK2128248 T4 DK 2128248T4 DK 09166406 T DK09166406 T DK 09166406T DK 2128248 T4 DK2128248 T4 DK 2128248T4
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oligonucleotides
oligonucleotide
target
rna
cells
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DK2128248T3 (da
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Margaret F Taylor
Tod M Woolf
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Life Technologies Corp
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Claims (10)

1. Fremgangsmåde til fremstilling af en oligonukleotidsammensætning, der omfatter: kombinering af 3, 4, 5, 6, 7 eller 8 forskellige dobbeltstrengede RNA-oligonukleotider, der er målrettet mod mindst to forskellige nukleotidsekvenser i et målgen, hvor de forskellige dobbeltstrengede RNA-oligonukleotider hver er til stede ved den samme koncentration eller er til stede ved forskellige koncentrationer, og hvor oligonukleotiderne omfatter mellem 15 og 30 nukleomonomerer.
2. Fremgangsmåde ifølge krav 1, hvor mindst ét af oligonukleotiderne omfatter mindst én modificeret internukleosidbinding.
3. Fremgangsmåde ifølge krav 1, hvor mindst ét af oligonukleotiderne omfatter mindst én modificeret sukkerdel.
4. Fremgangsmåde ifølge krav 1, hvor fremgangsmåden omfatter kombinering af 3 forskellige dobbeltstrengede RNA-oligonukleotider, der er målrettet mod tre forskellige nukleotidsekvenser i et målgen, hvor de forskellige dobbeltstrengede RNA-oligonukleotider hver er til stede ved den samme koncentration eller er til stede ved forskellige koncentrationer.
5. Fremgangsmåde ifølge krav 1, hvor fremgangsmåden omfatter kombinering af 4 forskellige dobbeltstrengede RNA-oligonukleotider, der er målrettet mod fire forskellige nukleotidsekvenser i et målgen, hvor de forskellige dobbeltstrengede RNA-oligonukleotider hver er til stede ved den samme koncentration eller er til stede ved forskellige koncentrationer.
6. Fremgangsmåde ifølge krav 1, hvor de forskellige dobbeltstrengede RNA-oligonukleotider hver er til stede ved den samme koncentration.
7. Fremgangsmåde ifølge krav 1, hvor de forskellige dobbeltstrengede RNA-oligonukleotider hver er til stede ved forskellige koncentrationer.
8. Fremgangsmåde ifølge krav 1, hvor de forskellige dobbeltstrengede RNA-oligonukleotider syntetiseres, så de omfatter forskellige kemier.
9. Fremgangsmåde ifølge et hvilket som helst af ovennævnte krav, hvor mindst ét af oligonukleotiderne er kovalent koblet til et steroid.
10. Fremgangsmåde ifølge krav 9, hvor steroidet er cholesterol.
DK09166406.0T 2002-02-01 2003-02-03 Oligonukleotidsammensætninger med forøget effektivitet DK2128248T4 (da)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US35320302P 2002-02-01 2002-02-01
US35338102P 2002-02-01 2002-02-01
US43623802P 2002-12-23 2002-12-23
US43860803P 2003-01-07 2003-01-07
EP20030707687 EP1478656B1 (en) 2002-02-01 2003-02-03 Oligonucleotide compositions with enhanced efficiency

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DK2128248T3 DK2128248T3 (da) 2011-06-14
DK2128248T4 true DK2128248T4 (da) 2017-03-13

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US (9) US20040014956A1 (da)
EP (10) EP2221377B2 (da)
JP (2) JP2005515780A (da)
AT (4) ATE556714T1 (da)
CA (2) CA2474910A1 (da)
DE (2) DE60337040D1 (da)
DK (1) DK2128248T4 (da)
WO (2) WO2003064626A2 (da)

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