JP2952041B2 - 培養ダイズ細胞のａｇｒｏｂａｃｔｅｒｉｕｍ媒介形質転換の改良法 - Google Patents

培養ダイズ細胞のａｇｒｏｂａｃｔｅｒｉｕｍ媒介形質転換の改良法

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JP2952041B2
JP2952041B2 JP6504746A JP50474693A JP2952041B2 JP 2952041 B2 JP2952041 B2 JP 2952041B2 JP 6504746 A JP6504746 A JP 6504746A JP 50474693 A JP50474693 A JP 50474693A JP 2952041 B2 JP2952041 B2 JP 2952041B2
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エイ． タウンセンド，ジェフリー
エイ． トーマス，ローリー
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Description

【発明の詳細な説明】 発明の分野 本発明は、Agrobacterium媒介形質転換によるトラン
スジェニックダイズ植物の生成に関する。
発明の背景 ダイズ（Glycine max）は、世界で最も重要な作物植
物の１つである。油およびタンパク質の生産のために、
5000万ヘクタールを超えて生育されている１年生マメ科
植物である。作物の生産価値は、200億ドルを越えると
評価される。毎年、１億メートルトンを超えるダイズが
生産される。マメ科植物の遺伝子転移技術の開発は、耐
病性、除草剤に対する抵抗性、および栄養価を増大する
ように改良した新規な栽培品種の開発が容易となるた
め、商業的に重要である。しかし、ダイズの改良のため
の分子学的アプローチは、トランスジェニックダイズ植
物を生成するのに利用可能な技術に制限される。
いくつかの重要な作物植物を包含する多くの植物は、
Agrobacterium媒介遺伝子転移を用いて遺伝的に改変さ
れている。McCormickら，（1986）Plant Cell Rep.5,81
−84;Radkeら，（1988）Theor.Appl.Genet.75,685−69
4;Umbeckら，（1987）Bio/Technology 5,263−266;Bott
emanおよびLeemans,（1988）Trend in Genetics 4,219
−222。不運にも、これらの双子葉植物種のいくつかの
遺伝子型はAgrobacterium感染に感受性であっても（Fac
ciottiら，（1985）Biotechnology 3,241−246;Owensお
よびCress（1985）Plant Physiol.77,87−94;Byrneら，
（1987）Plant Cell Tissue and Organ Culture 8,3−1
5）、形質転換にAgrobacteriumを使用することは、利用
可能で有効な形質転換および再生の手順がないことによ
り制限される。
最近まで、ダイズはAgrobacteriumの宿主範囲外にあ
ると考えられていた。DeCleeneおよびDeLey（1976）Bac
terial Rev.42,389−466。最近の報告では、ダイズは、
ダイズ遺伝子型およびAgrobacterium株に依存して、Agr
obacteriumに対して感受性が制限されていることを示唆
した。Byrneら，（1987）Plant Cell Tissue Organ Cul
t.8,3−15。たった１種のダイズのみ（Peking）が形質
転換に成功しているが、この変種は市場価値がない。こ
の形質転換可能な変種のわずかなトランスジェニックダ
イズ植物がAgrobacteriumと再生可能な外植片との共存
培養により生成されている。Hincheeら，（1988）Bio/T
echnology 6,915−922。Agrobacterium媒介法によるダ
イズの形質転換が市販可能になるためには、この方法
は、より抜きの商業的栽培品種の直接的形質転換が可能
になるようにしなければならない。
これらの問題に加えて、ダイズは再生が最も困難なGl
ycine種であることが知られている。外植片は根を容易
に生成せず、さらにトランスジェニック材料の栽培を行
うことはほとんど不可能である。このため、形質転換お
よび再生に用いられる方法は、現在まで信頼性がなく効
果がないままであった。
従って、本発明の目的は、植物細胞に感染し、その植
物細胞にＴ−DNAを転移してそこで発現させる、Agrobac
terium種の改良法を提供することである。
本発明の別の目的は、ダイズ植物細胞に感染し、この
細胞を形質転換するAgrobacterium種の能力を高めるた
めの、この種の新規な誘導培地を提供することである。
本発明のさらに別の目的は、ダイズ外植片から完全植
物への再生のための、新規な成長培地および方法を提供
することである。
本発明のプロセスは、ダイズ組織でのAgrobacterium
媒介形質転換技術およびそのトランスジェニックダイズ
植物への再生において大きな改良を示す。
発明の要旨 本発明に従って、トランスジェニックダイズ植物の生
成のための簡単で迅速な信頼性のあるプロセスが提供さ
れる。方法は、より抜きの商業的栽培品種を包含するす
べてのダイズ変種に有効であり、今までに用いられたシ
ステム以上に相当な改良を示す。これは、この方法が、
ダイズの形質転換および再生に必要でありこれまで欠け
ていた要因を提供し、そして健常で可稔性のトランスジ
ェニック植物生成を成功させるために、これらおよび他
の要因を最適化するからである。
本発明は、以下の工程を包含する、遺伝子型に依存し
ないトランスジェニックダイズ植物の生成方法を包含す
る： （ａ）発芽させたダイズ種子の下胚軸または培養子葉節
由来の外植片を、キメラ遺伝子を含むAgrobacterium種
と共に、アセトシリンゴン、α−ヒドロキシアセトシリ
ンゴン、アセトバニロン、シリンガアルデヒド、シリン
ガ酸、およびシナピン酸、ならびにそれらの混合物から
なる群から選択されるシグナル化合物の存在下で共存培
養する工程； （ｂ）28℃より低い温度での共存培養によりAgrobacter
iumの毒性を誘導する工程；および （ｃ）植物培養培地のpHをpH6.0より低く低下させるこ
とによりAgrobacteriumの毒性を誘導する工程。
このプロセスは、ダイズ下胚軸または子葉節外植片
と、目的の１つまたは複数の遺伝子を挿入されるプラス
ミドを有するAgrobacterium種との共存培養を包含す
る。ダイズ外植片は、供給植物から採取され、培養にお
いてカルスを生成し得る一片のダイズ組織である。ダイ
ズ下胚軸組織は、子葉の下部で根の上部のダイズ植物胚
または実生の軸の部分である。子葉は胚葉であり、そし
て子葉節は胚軸と子葉との間の実生部であり、これは下
胚軸と上胚軸との区別を植物学的に規定する（胚シュー
ト）。
培養ダイズ細胞の形質転換にかなり影響するいくつか
の要因が、本発明の達成において同定された。これらの
うち最も重要なことは、共存培養の間シグナル分子の正
しい使用によるAgrobacteriumの毒性（vir）遺伝子の誘
導であると思われる。培養ダイズ細胞は、形質転換プロ
セスを開始させるのに必要なシグナル分子を有さない
か、または限られた量で有する。これらの結果は、一般
に、ダイズ形質転換のためのvir遺伝子の導入の重要性
を認めているがこの問題を解決していない他の研究と一
致する。Delzerら，（1990）Crop Sci.30,320−322;Owe
nsおよびSmigocki（1988）Plant Physiol.88,570−57
3。本発明は、アセトシリンゴン（傷害を受けた植物細
胞により生成されるフェノール性化合物）を用いて、vi
r遺伝子を誘導する。他のフェノール性化合物、α−ヒ
ドロキシアセトシリンゴン、アセトバニロン、シリンガ
アルデヒド、シリンガ酸、およびシナピン酸もまたvir
遺伝子を誘導し、独立してまたはアセトシリンゴンと組
み合わせたそれらの使用により、ダイズ形質転換の効果
が改良され得る。共存培養プロセスで十分な量のシグナ
ル分子を使用することにより、あらゆる場合において、
形質転換頻度が高められた。
共存培養の温度が別の重要な要因であることが見出さ
れた。ダイズ細胞培養の通常の温度（26〜28℃）は、有
効な形質転換には不適切である。温度をより低くするこ
とにより、より有効な形質転換が行われる。28℃で観察
された阻害は、より高い温度の最近の過増殖およびその
結果生じる植物細胞の生存の低下から生じるようではな
かった。これは、硫酸カナマイシンを培地から除去した
とき、両温度で共存培養した外植片は大きなカルスを生
成したからである。共存培養中の培地pHもまた、形質転
換率に影響する。低pH（pH 5.5）に緩衝化するような処
理により高い効果が確実となる。理論により制限される
ことを意図しないが、これらの改良効果はシグナル分子
反応作用に関連すると思われる。vir遺伝子誘導は温度
およびpHに依存し、最適条件は約20℃およびpH 5.5であ
ることが知られている。Alt−Moerleら，（1988）Mol.G
en.Genet.213,1−８。
ダイズ細胞の形質転換の成功はまた、接種物における
細菌の濃度にも依存する。一般に、細菌数が大きくなる
ことにより、より多くの形質転換が達成される。飽和濃
度の細菌が存在し得るが、ここではダイズ細胞ではさら
なる形質転換は生じず、この数は非常に大きいと思われ
る。本発明者らの実験は、この数が、３×108生存細胞/
mlで、あるいはそれを超える濃度で細菌を１回30分接種
することにより行い得、そして３×107細胞/mlの従来濃
度（Deblaereら，（1987）Meth.Enzymology 153,277−2
93）では、かなり低すぎることを示唆した。
しかし、本発明者らの実験では、真の接種飽和条件は
特定され得なかった。連続接種により形質転換可能なダ
イズ細胞は、接種が非常に高い細菌濃度で行われるとき
であっても標的されることがないことが示された。高濃
度の細菌を接種しようと試みるときに直面する問題の１
つは、細菌細胞の凝集の傾向であった。凝集により、植
物細胞に付着するのに用いられ得る細菌数が減少した。
沈降した細菌を再懸濁するとすぐに凝集が始まり、この
凝集率は濃度依存性であった。これはまた、培地の機械
的混合の程度にも依存した。共存培養培地（細菌が再懸
濁される）の組成もまた凝集に影響し得る。従って、植
物細胞の急速な分裂の誘導およびAgrobacterium vir遺
伝子の高レベル誘導に適切な完全植物細胞培地のみがこ
のプロセスにおいて用いられた。
形質転換頻度の相当な増大は、凝集現象を制限し、そ
の効果による接種条件を用いて達成された。第一に、接
種は、混合を最小限にして、室温で高い開始濃度で行っ
た。第二に、それらは、各外植片に対し新しく再懸濁し
た対数増殖期のAgrobacteriumのペレットを接種するよ
うに、バッチ式で行った。外植片は、可能な限り最も高
い濃度での利用可能な細菌の存在下で（すなわち、個々
の細胞数をかなり減少させる凝集をする前に）、接種物
で傷つけた。
第三に、接種期間は、所定のバッチの最後の外植片を
傷害してから開始して30分ぐらいとした。時間をこれよ
り長くしても形質転換頻度を増大することはなかった。
連続接種により、頻度を適度に増大させた。この増大
は、おそらく植物細胞を形質転換するのに利用可能な細
菌の数の増加によると思われた。これらの実験から、ダ
イズ細胞は、高濃度のAgrobacteriumに長期間さらされ
ることに耐え得ることが明らかである。
vir遺伝子誘導を確実にする条件を用い、必要な高濃
度のAgrobacteriumを提供することにより、本発明者ら
は、初めて高頻度のダイズ形質転換を一貫して達成する
ことができた。本発明の方法により、いくつかの異なる
Agrobacterium株でこれらの頻度を得、そしてこの方法
は、Agrobacterium感染に対するダイズ変種の本来の感
受性により最低限の影響を受けるのみである。
形質転換後、外植片は、液体逆選択培地で培養され、
次いて固形の選択培地に移される。このプロセスは、gu
sのようなトランスジェニックマーカーによる同定に関
して、当該分野で記載されるように繰り返される。McCa
beら、（1988）Bio/Technology 6,922−926。シュート
は、トランスジェニック外植片で公知の方法により誘導
される。Wrightら，（1986）Plant Cell Reports 5,150
−154;Barwaleら，（1986）Planta 167,473−481。シュ
ートを切り取り、そしてピログルタミン酸をホルモン富
化成長培地に加えることにより、根が容易に誘導され
る。これは、根はダイズ組織からまれにしか誘導され
ず、従ってホルモンフリー培地でのみ誘導されるという
報告に反する。完全な成熟再生植物は、土壌での温室栽
培に移された後に生成される。
発明の詳細な説明 本発明者らは、gus遺伝子を有するAgrobacterium tu
mefaciensとの共存培養後、ダイズ下胚軸細胞においてE
scherichia coliからβ−グルクロニダーゼ（gus）遺
伝子を発現させた。キメラのgusおよびネオマイシンホ
スホトランスフェラーゼII（npt II）遺伝子を有するバ
イナリープラスミドを中に含んでいる無害化したAgroba
cterium株が、DNA転移のためのベクターであった。共存
培養後、表１に挙げた市販栽培品種由来のダイズ下胚軸
外植片を、100μm/ml硫酸カナマイシンを含有するカル
ス誘導培地（Hincheeら，（1988）Bio/Technology 6,91
5−922）で培養した。形質転換は、下胚軸外植片がgus
活性に対して組織化学的にアッセイされたときに、共存
培養後３週間に評価づけされた。形質転換は、解剖顕微
鏡（倍率10×）を用いて可視化された。これらの形質転
換は、染色植物細胞のセクター（sector）として現れ
た。gus遺伝子のダイズ染色体への安定した組み込み
は、染色セクター内の子孫の細胞集団における遺伝子活
性の保持により示唆された。複数の別個の事象がいくつ
かの外植片で検出され得た。これらは、gus遺伝子を発
現しない細胞により至る所で飛び出した。移植片の組は
共に接種され、共存培養され、選択され、そして組織化
学的にアッセイされた。各組は独立した実験単位であ
り、遺伝的差異（種子ロット内の）、外植片の配置（幼
植物内の）、および外植片の大きさによる変化を補うの
に十分な外植片数である。処理は、その中の外植片で検
出された事象の総数を記録することにより評価された。
この方法を用いて、持続した分裂が可能な形質転換細胞
のみが評価された。この測定値は、バックグラウンドgu
s活性およびあらゆる残留細菌混入とも明らかに区別さ
れた。
シグナル分子。表１は、８つの異なるダイズ変種の下胚
軸外植片を、アセトシリンゴン（100μＭ）の存在下お
よび不在下で、接種し（30分）そして共存培養した（３
日）実験の結果を示す。接種物は、最終濃度３×108生
存細胞/mlで、アセトシリンゴンを添加してまたは添加
しないで、10mM MESでpH 5.5に緩衝化した液体植物細胞
培地中に、対数期Agrobacteriumを再懸濁することによ
り調製された。外植片は、接種物中で調製され、そこに
30分間保持され、次いで３日間共存培養のために寒天固
形化培地に移された。共存培養は22℃で行った。共存培
養後、外植片は洗浄され、形質転換植物細胞の選択およ
びAgrobacteriaの逆選択のために抗生物質（カナマイシ
ン）を含有する固形培地に移された。継代培養は28℃で
あった。形質転換セクターは、アセトシリンゴンが接種
培地および共存培養培地に添加されたときにのみ生成さ
れた。形質転換セクターは、すべての変種で生成され
た。各変種（48外植片）で生成された形質転換セクター
の平均数は、107であった。形質転換セクター数は、最
低44（Cartter変種）から最高153（9391変種）までの範
囲であった。継代実験では、少数の形質転換セクター
が、アセトシリンゴンを経験しなかった外植片で生成さ
れたが、このような事象の頻度は低すぎて実際に測定す
ることはできなかった。
一晩細菌培養および植物培地を用いる細菌プレ培養期
にアセトシリンゴンを含めても、形質転換頻度は増大し
なかった。単糖類グルコースが接種培地および共存培養
培地に取り入れられたとき、同様の結果が得られた（デ
ータを示さず）。すべての継代実験は、接種培地および
共存培養培地に100μＭでアセトシリンゴンを含んで行
った。
温度。表２は、共通ペトリ皿に接種される下胚軸外植片
を無作為に２組に分けた実験の結果を示す。この組（各
24外植片）を、固形培地上で異なる温度（22℃および28
℃）で、３日間共存培養した。共存培養後、すべての外
植片を28℃で培養した。12回の別個の接種は、9341変種
を用いて、３×108生存細胞/mlのAgrobacteriumで30分
間行われた。22℃で共存培養した24個の外植片で生成さ
れた形質転換セクターの平均数は109であった。28℃形
質転換セクターの平均数は1.4であった。この実験にお
いて、低温での共存培養から生じる形質転換頻度の増大
は、約80倍である。すべての継代共存培養は22℃で行っ
た。
pH。表３は、外植片を異なるpHレベル（5.5、5.75、お
よび6.0）で接種し共存培養した実験の結果を示す。14
回の別個の接種は、各pHで行われた。培地を10μM MES
で緩衝化してpHの安定を確実にした。次いで共存培養
が、同様の緩衝化した固形化培地で３日間行われた。pH
6.0で24外植片で生成された形質転換セクターの平均数
は、pH 5.5（286）またはpH 5.75（288）のいずれかで
生成されたよりも有意に低かった（203）。これは、公
表された結果では、アセトシリンゴンを用いたときに、
Glycine maxにおいてpHの表示効果が全く示されなかっ
たことに反する。Godwinら，（1991）Plant Cell Rep.
9,671−675。すべての継代接種および共存培養は、pH
5.5に調整され、10μM MESで緩衝化された培地で行っ
た。
接種物における生存細菌濃度。表４は、外植片に異なる
濃度の細菌を接種した２つの実験の結果を示す。これら
の実験に関し、対数期の一晩細菌培養物を沈降し、次い
で適切な濃度への順次希釈により再懸濁した。各実験に
おいて、３つの別個の細菌希釈系列を共通一晩培養から
調製した。前述の実験におけるように、48個の外植片を
各接種物において調製し、そこで30分間保持し、固形培
地で３日間共存培養した。48個の外植片の各接種に対し
て生成された形質転換セクターの総数を記録した。最初
の３つの接種濃度:3×107、108、および３×108細胞/ml
について、より多くの形質転換セクターが、より多い細
菌により生成された。これは、両実験およびすべての希
釈系列に関していえる。
より高い接種濃度（109細胞/ml）では、形質転換頻度
の増大は検出されなかった。この濃度で生成されたセク
ターの平均数は、それ以下の濃度の平均数と有意に違わ
なかった。従って、投与量／反応プロフィールは、細菌
数が制限される濃度から細菌が飽和である濃度まで、３
×108細胞/mlで変化する。
所定の濃度で生成される形質転換体の絶対数は、実験
間でかなり変化する。各実験においてプラトーが見かけ
上達成されるので、Agrobacteriaの数よりもむしろその
状態が限定要因となるようである。異なるダイズ外植片
調製物が形質転換の能力により変化したことも考えられ
る。第三に考えられることは、接種の真の長さが実験間
で変化していることである。これはありそうにないこと
であるが、実験は、所定の濃度を飽和させるのに十分な
接種期間を規定して行った。30分の期間は、最大数の事
象を生じるように決定され、さらに時間を加えても何の
効果も生じなかった（データ示さず）。
連続接種。Agrobacteriaをダイズ細胞に感染させる試み
において、48個のダイズ下胚軸外植片（9341変種）の組
を、５×108生存細胞/mlで細菌を30分間、１回または２
回接種した実験を行った。すべての外植片は３日間共存
培養した。結果は表５に示す。形質転換セクターの平均
数は、２回処理（315）の方が１回処理（286）よりも有
意に高かった。連続接種により、さらに形質転換は生じ
得ないようである。形質転換可能なダイズ細胞プールの
真の飽和は１回接種により達成され、そして生存細菌数
は、接種が非常に高濃度でなされたときであっても制限
されたままである。しかし、形質転換に至る連合の大部
分は、最初の30分接種で生じている。
感受性および耐性ダイズ遺伝子型。表１に示した結果
は、試験したすべてのダイズ変種は、本発明の方法を用
いてAgrobacteriumにより形質転換され得ることを示し
た。Agrobacterium媒介形質転換に対するダイズ変種の
相対的感受性を決定するために、推定耐性変種（Corsoy
79およびCentury）の交雑組み合わせ検定を、感受性パ
ートナーとしてPeking変種を用いて行った。接種は、異
なる変種の外植片が共通の接種物において調製され得る
ように、ステンレスメッシュ分離器（0.5mm孔）を有す
るペトリ皿で行った。「耐性」変種の24個の外植片を、
等しい数の「感受性」外植片を含む各12個の別個のペト
リ皿中で調製した。結果は表６に示す。変種Pekingは、
変種Centuryの約２倍の頻度および変種Corsoy 79の約３
倍の頻度で形質転換された。変種に依存する感受性がこ
れらの実験により確認されているが、その程度（２〜３
倍）は小さいので、多様なダイズ生殖質の形質転換にAg
robacteriumを用いることに関して重大な示唆を与える
ほどではない。
Agrobacterium株。２つの互いの無害化されたAgrobacte
rium株と、オクトパイン（octopine）株であるLBA4404
とを、ダイズ下胚軸細胞の形質転換を媒介する能力につ
いて比較した；それらは、L,L−スクシナモパイン（suc
cinamopine）株EHA 101およびノパリン（nopaline）株C
58−pz707であった。表７は、これらの各株の濃度を増
大させて、Williams 82の下胚軸外植片への接種に用い
る実験の結果を示す。48個の外植片を各濃度の各株に接
種した。LBA4404をコントロールとして３×108生存細胞
/mlで含有させた。両方の株で形質転換セクターが生じ
た。両方の場合において、形質転換頻度は、接種物にお
ける細菌濃度に依存し、細菌数が多いほど形質転換は多
く生じた。試験したレベルでは、形質転換において、ど
ちらもLBA4404と比較して相当な変化はみられなかっ
た。
以下の実施例は、本発明に従って種々の適用を説明す
るが、決して本発明の範囲を限定する意図はない。
実施例１ ダイズ（Glycine max）種子（Pioneeer変種9341）
は、鐘状ガラス容器中で放出された塩素ガスに曝すこと
により表面を滅菌した。ガスを、3.5mlの塩酸（34〜37
％w/w）を100mlの次亜塩素酸ナトリウム（5.25％w/w）
に加えることにより作製した。約１立方フィートの容量
のコンテナ中で16〜20時間曝した。表面滅菌した種子
を、室温でペトリ皿中に保存した。種子は、植物成長調
節剤を含まないGamborgに従う1/10強度の寒天固形化培
地［最小有機物を含むB5基本培地、Sigma Chemical C
o.,カタログ番号G5893、0.32gm/L;ショ糖、0.2％w/vお
よび２−［Ｎ−モルホリノ］エタンスルホン酸（ME
S）、3.0mM］のプレートに置き、28℃で昼の長さを16時
間にして、約20μEm2S1の弱い白色蛍光照明をあてて培
養することにより、発芽させた。３、４日後、主を共存
培養用に調製し得た。種子の外皮を取り除き、伸長して
いる幼根を子葉の下部３〜4mmで取り除いた。いくつか
のペトリ皿のそれぞれにつき10個の得られた種子を保持
した。
実施例２ 1.0μg/mlのテトラサイクリンを含む最小Ａ培地で対
数期へと増殖した、バイリープラスミドp12GUSBN17（DP
1816）またはp12−4X（DP1813）を有するAgrobacterium
tumefaciens LBA 4404株の終夜培養物をプールし、55
0nmでの吸光度測定を行った。かなりの容量の培養物
を、沈澱して1.0×1010細胞と2.0×1010細胞との間の細
胞が各管に集められるように、15mlの円錐形遠沈管に入
れた。ここでO.D.550 1.0＝1.4×109細胞/mlである。沈
澱は6000gで10分間の遠心により得た。遠心後、上清を
デカントし、接種物が必要になるまで（しかし１時間は
超えない）管を室温で保持した。
実施例３ 接種を、各プレートの種子を新たに再懸濁したAgroba
cteriumのペレットで処理するようなバッチで行った。
ペレットを20mlの接種培地中に一度に再懸濁した。接種
培地は、B5塩（G5893）、3.2gm/L;ショ糖、2.0％w/v;6
−ベンジルアミノプリン（BAP）、44μM;インドール酪
酸（IBA）、0.5μM;アセトシリンゴン（AS）、100μＭ
からなり、10mMのMESでpH5.5に緩衝化した。再懸濁はボ
ルテックスにより行った。次いで接種物を、調製した種
子を含むペトリ皿に注ぎ、子葉節を外科用メスで浸軟さ
せた。これは、２つの子葉全体を保護する茎頂（shoot
apex）を通り、経線部分で半分に種子を分けることによ
り行った。次いで２つの半分の茎頂は、外科用メスでそ
れらを取り除くことによりそれぞれの子葉を分解した。
次いで子葉節を、外科用メスで対称な尖にそって切れ目
を繰り返し付けることにより浸軟した。外植片から軸外
側をまったく切断しないように気を付ける。20個の外植
片をざっと５分間で調製し、次いで室温で30分間撹拌せ
ずにインキュベートした。追加のプレートをこの間に調
製した。外植片を0.2％w/vのGelrite（Merck ＆ Co.,In
c.）で固形化させた同じ培地のプレートに移して30分後
に、この外植片を軸方向側を上にして培地の表面層に埋
め、22℃で３日間、約20μEm2S1の弱い白色蛍光下で培
養した。
実施例４ ３日後に、外植片を液体逆選択培地に移した。逆選択
培地は、B5塩（G5893）、3.2gm/L;ショ糖、2.0％w/v;BA
P、5.0μM;IBA、0.5μM;バンコマイシン、200μg/ml;セ
フォタキシム、500μg/mlからなり、3mMのMESでpH5.7に
緩衝化した。10個の外植片を各ペトリ皿中で、一定のゆ
っくりした旋回撹拌しながら室温で４日間洗浄した。逆
選択培地は４回交換した。
実施例５ 次いで外植片を寒天固形化選択培地に取り出した。選
択培地は、B5塩（G5893）、3.2gm/L;ショ糖、2.0％w/v;
BAP、5.0μM;IBA、0.5μM;硫酸カナマイシン、50μg/m
l;バンコマイシン、100μg/ml;セフォタキシム、30μg/
ml;チメンチン（timentin）、30μg/mlからなり、3.0mM
のMESでpH5.7に緩衝化した。選択培地を0.3％w/vのSeaK
emアガロースで固形化した。外植片を軸方向を下にして
培地に埋め、28℃で昼の長さを16時間にして60〜80μEm
2S1の弱い白色蛍光照明をあてて培養した。
実施例６ ２週間後、外植片を再び旋回シェーカー上で液体培地
で洗浄した。今回は、洗浄を50μg/mlの硫酸カナマイシ
ンを含む逆選択培地中で終夜で行った。次の日、外植片
を寒天固形化選択培地に取り出した。再びそれらを培地
中に軸方向を下に埋めた。培養をもう２週間、前のよう
に行った。
実施例７ １カ月後選択培地上で、経質転換組織は、バックグラ
ウンドの退色したあまり健常ではない組織に対して緑色
セクターの再生組織として認められるようになった。緑
色セクターのない外植片を放棄し、緑色セクターのある
外植片を伸長培地（elongation medium）に移した。伸
長培地は、B5塩（G5893）、3.2gm/L;ショ糖、2.0％w/v;
IBA、3.3μM;ジベレリン酸、1.7μM;バンコマイシン、1
00μg/ml;セフォタキシン、30μg/ml;およびチメンチ
ン、30μg/mlからなり、3.0mMのMESでpH5.7に緩衝化し
た。伸長培地は、0.2％w/vのgelriteで固形化した。そ
れらは、軸方向を上にして埋め、前のように培養した。
培養を、この培地で２週間おきに新鮮なプレートに移し
て継続した。シュートが0.5cmの長さになった場合、そ
れを根元で切除し、13×100mmの試験管中の発根培地に
置いた。発根培地は、B5塩（G5893）、3.2gm/L;ショ
糖、15gm/L;ニコチン酸、20μM;ピログルタミン酸（PG
A）、900mg/L、およびIBA、10μＭからなっていた。そ
れは、3.0mMのMESでpH5.7に緩衝化し、0.2％w/vのGelri
teで固形化した。10日後、シュートをIBAまたはPGAを含
まない同培地に移した。シュートを発根させ、前と同じ
環境条件下でこれらの管内に保持した。
実施例８ 根系がうまく樹立した場合、幼植物をプラントコン
（plant con）（ICN Biomedicals,Inc.,カタログ番号26
−720＆１−02）の中の滅菌した混合土に移した。温
度、日長、および光強度は前のまま維持した。これらの
条件下で、再生植物は、育ちが良く（小さいが）ほとん
ど正常の植物となった。それらの根系が再びうまく樹立
された場合、プラントコンのコーナーを切り落とし、植
物を環境チャンバーまたは温室で徐々に寒気に慣らし
た。最終的に、それらは混合土に鉢上げし、温室で成熟
して種子を生じるまで生育させた。
───────────────────────────────────────────────────── フロントページの続き (56)参考文献 Ｐｌａｎｔ Ｃｅｌｌ Ｒｅｐｏｒｔ ｓ ９（1991）Ｐ．671−675 (58)調査した分野(Int.Cl.6，ＤＢ名) C12N 15/09 C12N 5/00 C12N 1/21 A01H 1/00 ＢＩＯＳＩＳ（ＤＩＡＬＯＧ) ＷＰＩＬ（ＤＩＡＬＯＧ)

Claims (7)

    (57)【特許請求の範囲】
  1. 【請求項１】以下の工程を包含する、遺伝子型に依存し
    ない、トランスジェニックダイズ植物の生成方法： a. 発芽させたダイズ種子の下胚軸または培養子葉節由
    来の外植片を、108から３×108細胞/mlの濃度のキメラ
    遺伝子を含むAgrobacterium種細胞と共に、アセトシリ
    ンゴン、α−ヒドロキシアセトシリンゴン、アセトバニ
    ロン、シリンガアルデヒド、シリンガ酸、シナピン酸、
    およびそれらの混合物からなる群から選択されるシグナ
    ル化合物の存在下で共存培養する工程； b. 18〜28℃の共存培養温度を維持する工程；および c. 植物培養培地pHをpH6.0未満にまで低下させること
    により該Agrobacteriumの毒性を誘導する工程。
  2. 【請求項２】前記共存培養がAgrobacteriumの順次接種
    により行われる、請求項１に記載の方法。
  3. 【請求項３】前記キメラ遺伝子が種子貯蔵タンパク質遺
    伝子である、請求項１に記載の方法。
  4. 【請求項４】前記キメラ遺伝子がBertholletia excelsa
    由来の2S貯蔵タンパク質である、請求項１に記載の方
    法。
  5. 【請求項５】子葉節からダイズ植物を再生する工程をさ
    らに包含する、請求項１から４に記載の方法であって、
    以下の工程を包含する、方法： a. 該節を分割する工程； b. 該分割した節を、栄養培地上でカルス組織が発達す
    るまで培養する工程であって、該組織がシュートを含
    む、工程；および c. 該カルスから該シュートを切り取り、そしてホルモ
    ンおよびピログルタミン酸を含有する栄養培地上で該シ
    ュートを発根させて幼植物を形成する工程。
  6. 【請求項６】前記発根培地がGamborg培地である、請求
    項５に記載の方法。
  7. 【請求項７】前記幼植物が、土を含む培地に移され、該
    幼植物から完全植物が生じるまで該培地上で生育され
    る、請求項５に記載の方法。
JP6504746A 1992-07-27 1993-07-26 培養ダイズ細胞のａｇｒｏｂａｃｔｅｒｉｕｍ媒介形質転換の改良法 Expired - Lifetime JP2952041B2 (ja)

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Families Citing this family (734)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5416011A (en) * 1988-07-22 1995-05-16 Monsanto Company Method for soybean transformation and regeneration
US6605712B1 (en) * 1990-12-20 2003-08-12 Arch Development Corporation Gene transcription and ionizing radiation: methods and compositions
WO1995034667A2 (en) 1994-06-10 1995-12-21 Danisco A/S Transformation of guar
HUP9900864A3 (en) * 1995-05-31 2001-11-28 Pioneer Hi Bred Int Methods of increasing accumulation of essential amino acids in seeds
AR002176A1 (es) * 1995-06-02 1998-01-07 Pioneer Hi Bred Int Metodos mejorados destinados a la produccion de alimentos para animales, mediante la reduccion de los niveles proteinicos endogenados en el poroto.
GB9600698D0 (en) * 1996-01-13 1996-03-13 British American Tobacco Co Genetic transformation of trees
US5693512A (en) * 1996-03-01 1997-12-02 The Ohio State Research Foundation Method for transforming plant tissue by sonication
US5959179A (en) * 1996-03-13 1999-09-28 Monsanto Company Method for transforming soybeans
US6091002A (en) 1996-03-13 2000-07-18 Monsanto Company Polyhydroxyalkanoates of narrow molecular weight distribution prepared in transgenic plants
US6946588B2 (en) 1996-03-13 2005-09-20 Monsanto Technology Llc Nucleic acid encoding a modified threonine deaminase and methods of use
US5958745A (en) * 1996-03-13 1999-09-28 Monsanto Company Methods of optimizing substrate pools and biosynthesis of poly-β-hydroxybutyrate-co-poly-β-hydroxyvalerate in bacteria and plants
US7897843B2 (en) 1999-03-23 2011-03-01 Mendel Biotechnology, Inc. Transcriptional regulation of plant biomass and abiotic stress tolerance
DK1137790T3 (da) * 1998-12-11 2006-07-31 Monsanto Technology Llc En Agrobacterium-medieret plantetransformationsfremgangsmåde med forbedret effektivitet
EP1141356A2 (en) * 1998-12-23 2001-10-10 The Samuel Roberts Noble Foundation, Inc. Plant transformation process
CA2359868A1 (en) 1999-01-14 2000-07-20 Monsanto Company Soybean transformation method
US6429357B1 (en) 1999-05-14 2002-08-06 Dekalb Genetics Corp. Rice actin 2 promoter and intron and methods for use thereof
GB0002814D0 (en) 2000-02-09 2000-03-29 Univ York Nucleic acids and their uses
US6580019B1 (en) 2000-03-09 2003-06-17 Dekalb Genetics Corporation Non-reciprocal recombination-mediated transgene deletion in transgenic plants
US6750379B2 (en) * 2000-03-09 2004-06-15 Dekalb Genetics Corporation Homologous recombination-mediated transgene alterations in plants
KR100402513B1 (ko) * 2001-02-16 2003-10-22 주식회사 싸이젠하베스트 유전자 조작을 통한 식물체의 효과적인 형질전환 방법
US8034791B2 (en) 2001-04-06 2011-10-11 The University Of Chicago Activation of Egr-1 promoter by DNA damaging chemotherapeutics
ATE541937T1 (de) * 2001-04-06 2012-02-15 Univ Chicago Chemotherapeutische einleitung der egr-1-promoter-aktivität in gentherapie
US20040242523A1 (en) * 2003-03-06 2004-12-02 Ana-Farber Cancer Institue And The Univiersity Of Chicago Chemo-inducible cancer gene therapy
US20020188965A1 (en) 2001-04-20 2002-12-12 Zou-Yu Zhao Methods of transforming plants
WO2002095002A2 (en) 2001-05-22 2002-11-28 University Of Chicago N4 virion single-stranded dna dependent rna polymerase
EP2333070A3 (en) 2001-06-22 2011-09-07 Pioneer Hi-Bred International, Inc. Defensin polynucleotides and methods of use
JP3985474B2 (ja) * 2001-07-24 2007-10-03 日本製紙株式会社 遺伝子導入効率を向上させたユーカリ属の植物への遺伝子導入方法
EP1279737A1 (en) 2001-07-27 2003-01-29 Coöperatieve Verkoop- en Productievereniging, van Aardappelmeel en Derivaten AVEBE B.A. Transformation method for obtaining marker-free plants
US20030046733A1 (en) * 2001-09-06 2003-03-06 Dias Kalyani Mallika Transformation of soybeans
WO2003027243A2 (en) 2001-09-27 2003-04-03 Pioneer Hi-Bred International, Inc. Phytate polynucleotides and methods of use
US7338656B2 (en) 2001-10-26 2008-03-04 Baylor College Of Medicine Composition and method to alter lean body mass and bone properties in a subject
AR037778A1 (es) 2001-12-11 2004-12-01 Advisys Inc Suplementacion mediada por plasmido para el tratamiento de sujetos cronicamente enfermos
US20060009409A1 (en) 2002-02-01 2006-01-12 Woolf Tod M Double-stranded oligonucleotides
EP3415625A1 (en) 2002-02-01 2018-12-19 Life Technologies Corporation Double-stranded oligonucleotides
WO2003064621A2 (en) 2002-02-01 2003-08-07 Ambion, Inc. HIGH POTENCY siRNAS FOR REDUCING THE EXPRESSION OF TARGET GENES
US6948972B2 (en) * 2002-03-27 2005-09-27 Pent Technologies, Inc. Overhead lighting splitter
BRPI0311324B1 (pt) 2002-05-22 2020-01-28 Monsanto Technology Llc polinucleotídeo, vetor, polipeptídeo com atividade dessasturase, métodos para produção de planta e óleo de milho contendo ácidos graxos de ômega-3, para aumentar o valor nutricional de um produto comestível, de fabricação de alimento ou alimentação, bem como composição comestível
CN1668744A (zh) * 2002-06-22 2005-09-14 辛根塔参与股份公司 转化大豆的方法
US6916474B2 (en) 2002-07-15 2005-07-12 Board Of Regents, The University Of Texas System Antibodies with increased affinities for anthrax antigens
EP1551967B1 (en) 2002-07-19 2011-08-31 University Of South Carolina Compositions and methods for the modulation of gene expression in plants
EP2269619A1 (en) 2002-08-12 2011-01-05 Jennerex Biotherapeutics ULC Methods and compositions concerning poxviruses and cancer
WO2004074462A2 (en) 2003-02-20 2004-09-02 Athenix Corporation Delta-endotoxin genes and methods for their use
US7880061B2 (en) 2003-02-25 2011-02-01 The Curators Of The University Of Missouri Soybean variety SN97-6946
TW200424214A (en) * 2003-04-21 2004-11-16 Advisys Inc Plasmid mediated GHRH supplementation for renal failures
CA2521284C (en) 2003-04-29 2014-07-08 Pioneer Hi-Bred International, Inc. Novel glyphosate-n-acetyltransferase (gat) genes
CN103333885A (zh) 2003-06-23 2013-10-02 先锋高级育种国际公司 将单基因控制的保绿潜力工程化进植物中
US7150993B2 (en) 2003-08-05 2006-12-19 Monsanto Technology Llc Method for excision of plant embryos for transformation
US7560611B2 (en) * 2003-08-05 2009-07-14 Monsanto Technology Llc Method and apparatus for substantially isolating plant tissues
AU2004280634A1 (en) 2003-10-09 2005-04-21 E. I. Du Pont De Nemours And Company Gene silencing by using micro-RNA molecules
EP2333075B1 (en) 2003-12-16 2018-07-04 Pioneer Hi-Bred International Inc. Dominant gene suppression transgenes and methods of using same
US20070169227A1 (en) 2003-12-16 2007-07-19 Pioneer Hi-Bred International Inc. Dominant Gene Suppression Transgenes and Methods of Using Same
HUE028143T2 (en) 2004-02-25 2016-12-28 Pioneer Hi Bred Int New crystalline bacillus thuringiensis polypeptides, polynucleotides, and compositions thereof
US7459289B2 (en) 2004-03-08 2008-12-02 North Carolina State University Lactobacillus acidophilus nucleic acid sequences encoding carbohydrate utilization-related proteins and uses therefor
CN1968688A (zh) 2004-04-16 2007-05-23 孟山都技术有限公司 脂肪酸去饱和酶在玉米中的表达
CN101001957B (zh) 2004-06-09 2012-07-04 先锋高级育种国际公司 质体转运肽
AU2005327983B2 (en) 2004-06-30 2008-08-07 Pioneer Hi-Bred International, Inc. Methods of protecting plants from pathogenic fungi
MXPA06015054A (es) 2004-07-02 2007-08-07 Pioneer Hi Bred Int Polipeptidos antifungales.
EP1788861B1 (en) 2004-08-24 2017-04-12 Monsanto Technology, LLC Adenylate translocator protein gene non-coding regulatory elements for use in plants
US7989678B2 (en) 2004-09-02 2011-08-02 Basf Plant Science Gmbh Disarmed agrobacterium strains, Ri-plasmids, and methods of transformation based thereon
US20060064786A1 (en) 2004-09-17 2006-03-23 Pioneer Hi-Bred International, Inc. Isopentenyl transferase sequences and methods of use
EP1807522B1 (en) 2004-10-21 2013-11-20 Venganza Inc. Methods and materials for conferring resistance to pests and pathogens of plants
CA2857878A1 (en) 2004-11-12 2006-12-28 Asuragen, Inc. Methods and compositions involving mirna and mirna inhibitor molecules
CA2592894C (en) 2004-12-28 2013-08-27 Pioneer Hi-Bred International, Inc. Improved grain quality through altered expression of gamma-zein protein
EP1846442B1 (en) 2005-01-13 2010-04-21 Pioneer Hi-Bred International, Inc. Maize cyclo1 gene and promoter
WO2006091194A1 (en) 2005-02-23 2006-08-31 North Carolina State University Alteration of tobacco alkaloid content through modification of specific cytochrome p450 genes
MX2007010552A (es) 2005-03-02 2009-02-19 Inst Nac De Tecnologia Agropec Plantas de arroz resistentes a herbicidas, polinucleotidos que codifican proteinas de subunidad grande de acido acetohidroxi sintasa resistentes a herbicidas, y sus metodos de uso.
CA2605643C (en) 2005-05-02 2012-07-24 Purdue Research Foundation Methods for increasing the yield of fermentable sugars from plant stover
US7611898B2 (en) 2005-05-09 2009-11-03 The Samuel Roberts Noble Foundation Agrobacterium transformation of stolons
AR053269A1 (es) 2005-05-16 2007-04-25 Monsanto Technology Llc Plantas y semillas de maiz con mejoramiento de asparagina y proteina
JP5265356B2 (ja) 2005-07-01 2013-08-14 ビーエーエスエフ ソシエタス・ヨーロピア 除草剤耐性ヒマワリ植物、除草剤耐性のアセトヒドロキシ酸シンターゼ大サブユニットタンパク質をコードするポリヌクレオチド、及び使用方法
EP1907553B1 (en) 2005-07-18 2012-08-22 Pioneer Hi-Bred International Inc. Modified frt recombination sites and methods of use
CN101351213B (zh) * 2005-09-07 2013-10-02 詹纳里克斯公司 使用表达gm-csf的痘病毒系统性治疗转移性癌和/或全身散布性癌
WO2007030674A2 (en) * 2005-09-07 2007-03-15 Maxcyte, Inc. Use of nucleases to improve viability and enhance transgene expression in transfected cells
US8980246B2 (en) 2005-09-07 2015-03-17 Sillajen Biotherapeutics, Inc. Oncolytic vaccinia virus cancer therapy
ES2614943T3 (es) 2005-09-16 2017-06-02 Monsanto Technology Llc Procedimientos para el control genético de infestaciones de insectos en plantas y composiciones de los mismos
US8859846B2 (en) 2005-09-21 2014-10-14 E. I. Du Pont De Nemours And Company Doubling of chromosomes in haploid embryos
WO2007058833A2 (en) 2005-11-10 2007-05-24 Pioneer Hi-Bred International, Inc. Dof (dna binding with one finger) sequences and methods of use
WO2007087153A2 (en) * 2006-01-06 2007-08-02 University Of Georgia Research Foundation Cyst nematode resistant transgenic plants
US7663020B2 (en) * 2006-01-11 2010-02-16 Agrinomics Llc Generation of plants with altered oil content
US7622573B2 (en) 2006-01-17 2009-11-24 Biolex, Inc. Expression control elements from the lemnaceae family
EP1976883B1 (en) 2006-01-17 2012-10-03 Medarex, Inc. Monoclonal antibodies against cd30 lacking in fucosyl and xylosyl residues
EP1998800A2 (en) 2006-01-18 2008-12-10 University Of Chicago Compositions and methods related to staphylococcal bacterium proteins
US7868228B2 (en) 2006-01-31 2011-01-11 Monsanto Technology Llc Phosphopantetheinyl transferases from bacteria
US20070186313A1 (en) * 2006-01-31 2007-08-09 Pioneer Hi-Bred International, Inc. Method for high throughput transgene function analysis for agronomic traits in maize
AR059433A1 (es) 2006-02-10 2008-04-09 Monsanto Technology Llc Identificacion y uso de genes blanco para el control de nematodos parasitos de plantas
CN105385679B (zh) 2006-02-13 2020-05-26 孟山都技术有限公司 选择和稳定dsRNA构建体
US8237014B2 (en) * 2006-02-27 2012-08-07 Edenspace Systems Corporation Energy crops for improved biofuel feedstocks
US7820883B2 (en) * 2006-03-15 2010-10-26 Dow Agrosciences Llc Resistance to auxinic herbicides
BRPI0710455A2 (pt) 2006-04-19 2011-08-16 Pioneer Hi Bred Int polipeptìdeo isolado, polinucleotìdeo isolado e métodos para aumentar o nìvel de um polipeptìdeo em uma planta, para modular o nìvel de um polipeptìdeo em uma planta e para modificar o crescimento de uma planta
AR060563A1 (es) 2006-04-24 2008-06-25 Mendel Biotechnology Inc Promotores inducibles por enfermedad
WO2007134234A2 (en) 2006-05-12 2007-11-22 Monsanto Technology Llc Methods and compositions for obtaining marker-free transgenic plants
EP2803728B1 (en) 2006-05-16 2018-11-21 Monsanto Technology LLC Use of non-agrobacterium bacterial species for plant transformation
DE602007012343D1 (de) 2006-05-16 2011-03-17 Du Pont Antimykotische polypeptide
WO2007137114A2 (en) 2006-05-17 2007-11-29 Pioneer Hi-Bred International, Inc. Artificial plant minichromosomes
EP2500433A3 (en) 2006-06-29 2013-02-20 Mendel Biotechnology, Inc. Improved yield and stress tolerance in transgenic plants
EP2069791B1 (en) 2006-07-27 2012-01-25 University of Maryland, Baltimore Cellular receptor for antiproliferative factor
EP2046111B1 (en) 2006-08-07 2012-06-27 Mendel Biotechnology, Inc. Plants with enhanced size and growth rate
US8395020B2 (en) 2006-08-31 2013-03-12 Monsanto Technology Llc Methods for rapidly transforming monocots
CN102026645B (zh) 2006-09-15 2016-01-27 渥太华医院研究机构 溶瘤弹状病毒
EP2664673A3 (en) 2006-10-05 2014-02-19 E. I. du Pont de Nemours and Company Maize microRNA sequences
AU2007310975B2 (en) 2006-10-18 2012-09-27 Seminis Vegetable Seeds, Inc. Carrots having increased lycopene content
US8753840B2 (en) 2006-10-20 2014-06-17 Arizona Board Of Regents On Behalf Of Arizona State University Modified cyanobacteria
CA2988382A1 (en) * 2006-11-15 2008-05-22 Agrigenetics, Inc. Generation of plants with altered protein, fiber, or oil content
WO2008061156A2 (en) 2006-11-15 2008-05-22 Agrigenetics, Inc. Generation of plants with altered protein, fiber, or oil content
CA2669900C (en) 2006-11-15 2016-03-01 Agrigenetics, Inc. Generation of plants expressing an imq polypeptide and having altered protein, fiber or oil content
WO2008079545A2 (en) 2006-11-15 2008-07-03 Agrigenetics, Inc. Generation of plants with altered protein, fiber, or oil content
CA2669989A1 (en) 2006-11-15 2008-05-22 Agrigenetics, Inc. Generation of plants with altered protein, fiber, or oil content
ATE555128T1 (de) 2006-11-30 2012-05-15 Res Dev Foundation Verbesserte immunglobulin-bibliotheken
EP2114123A4 (en) * 2006-12-15 2010-08-11 Agrinomics Llc GENERATION OF PLANTS WITH CHANGED OIL, PROTEIN OR FIBER CONTENT
BRPI0721171A2 (pt) * 2006-12-15 2014-03-18 Agrinomics Llc Produção de plantas com conteúdo alterado de óleos, proteínas ou fibras
US7851673B2 (en) * 2006-12-15 2010-12-14 Agrinomics Llc Generation of plants with altered oil, protein, or fiber content
CN101600340A (zh) 2006-12-15 2009-12-09 农业经济有限责任公司 具有改变的油、蛋白或纤维含量的植物的生产
CA2672488C (en) * 2006-12-15 2017-06-13 Agrigenetics, Inc. Generation of plants with altered oil, protein, or fiber content
EP2132218B1 (en) * 2007-03-08 2015-04-22 The New Zealand Institute for Plant and Food Research Limited Transferases, epimerases, polynucleotides encoding these and uses thereof
AU2008226443B2 (en) 2007-03-09 2013-07-11 Monsanto Technology Llc Preparation and use of plant embryo explants for transformation
KR20080084528A (ko) 2007-03-15 2008-09-19 제네렉스 바이오테라퓨틱스 인크. 종양살상형 백시니아 바이러스 암 치료
US20100293664A1 (en) * 2007-04-03 2010-11-18 Sathish Puthigae Control of plant gene expression
UA126227C2 (uk) 2007-04-04 2022-09-07 Басф Плант Саєнс Гмбх Злакова рослина, толерантна до імідозолінону або сульфонілсечовини
NZ597328A (en) 2007-04-04 2014-03-28 Basf Se Herbicide-resistant brassica plants and methods of use
US7723582B2 (en) * 2007-04-12 2010-05-25 Dow Agrosciences Llc Canola cultivar DN041100
US7723579B2 (en) * 2007-04-12 2010-05-25 Dow Agrosciences Llc Canola cultivar DN040244
US7723580B2 (en) * 2007-04-12 2010-05-25 Dow Agrosciences Llc Canola cultivar DN040844
US7728195B2 (en) * 2007-04-12 2010-06-01 Dow Agrosciences Llc Canola cultivar DN040856
US7718852B2 (en) * 2007-04-12 2010-05-18 Dow Agrosciences Llc Canola cultivar DN040241
US7723577B2 (en) * 2007-04-12 2010-05-25 Dow Agrosciences Llc Canola cultivar DN040847
US7723581B2 (en) * 2007-04-12 2010-05-25 Dow Agrosciences Llc Canola cultivar DN040845
US7723578B2 (en) * 2007-04-12 2010-05-25 Dow Agrosciences Llc Canola cultivar DN040839
AU2008240142B2 (en) * 2007-04-12 2014-11-06 Corteva Agriscience Llc Novel canola cultivars having high yield and stabilized fatty acid profiles
DK2155789T3 (da) 2007-05-01 2013-10-21 Res Dev Foundation Immunoglobulin-Fc-biblioteker
CN101679493A (zh) 2007-05-25 2010-03-24 克罗普迪塞恩股份有限公司 在植物中通过调节玉米alfin的产量增强
EP2164863A4 (en) 2007-06-15 2010-07-28 Univ Arkansas METHOD FOR THE ADMINISTRATION OF MOLECULES IN CELLS USING A RICIN SUB-UNIT AND RELATED COMPOSITIONS THEREOF
CN101715291A (zh) * 2007-06-18 2010-05-26 农业经济有限责任公司 具有改变的油、蛋白或纤维含量的植物的生产
BRPI0816750A2 (pt) 2007-06-29 2015-09-29 Pioneer Hi Bred Int métodos para alterar o genoma de uma célula de planta monocotiledônea e para modificar uma sequência alvo genômica endógena específica e planta de milho
WO2009014462A1 (en) * 2007-07-26 2009-01-29 Sathish Puthigae Methods and polynucleotides for improving plants
JP5658564B2 (ja) 2007-08-31 2015-01-28 ザ・ユニバーシティ・オブ・シカゴＴｈｅ Ｕｎｉｖｅｒｓｉｔｙ Ｏｆ Ｃｈｉｃａｇｏ ブドウ球菌肺疾患および状態に対する免疫に関連した方法および組成物
CA2703701C (en) * 2007-10-31 2017-06-13 Hendrikus Antonius Rikkerink Resistance gene and uses thereof
US8124851B2 (en) 2007-11-12 2012-02-28 North Carolina State University Alteration of tobacco alkaloid content through modification of specific cytochrome P450 genes
US8115055B2 (en) 2007-12-18 2012-02-14 E.I. Du Pont De Nemours And Company Down-regulation of gene expression using artificial microRNAs
US8937217B2 (en) 2007-12-18 2015-01-20 E. I. Du Pont De Nemours And Company Down-regulation of gene expression using artificial microRNAs
CA2896367A1 (en) 2007-12-20 2009-07-09 Dow Agrosciences Llc Low saturated-fat sunflower and associated methods
US9605035B2 (en) 2008-01-10 2017-03-28 Research Development Foundation Vaccines and diagnostics for the ehrlichioses
US8367895B2 (en) 2008-01-17 2013-02-05 Pioneer Hi-Bred International, Inc. Compositions and methods for the suppression of target polynucleotides from the family aphididae
US8847013B2 (en) 2008-01-17 2014-09-30 Pioneer Hi Bred International Inc Compositions and methods for the suppression of target polynucleotides from lepidoptera
WO2009094647A2 (en) 2008-01-25 2009-07-30 Introgen Therapeutics, Inc. P53 biomarkers
NZ587653A (en) * 2008-04-03 2011-10-28 Satish Puthigae Promoter fragment isolated from Lolium perenne
US8487159B2 (en) * 2008-04-28 2013-07-16 Metabolix, Inc. Production of polyhydroxybutyrate in switchgrass
NZ568190A (en) 2008-05-12 2010-09-30 Nz Inst Plant & Food Res Ltd Chimeric compositions and methods for regulating plant gene expression
US8829282B2 (en) * 2008-05-14 2014-09-09 Monsanto Technology, Llc Plants and seeds of spring canola variety SCV425044
US7964774B2 (en) * 2008-05-14 2011-06-21 Monsanto Technology Llc Plants and seeds of spring canola variety SCV384196
US7947877B2 (en) * 2008-05-14 2011-05-24 Monosanto Technology LLC Plants and seeds of spring canola variety SCV328921
US7935870B2 (en) * 2008-05-14 2011-05-03 Monsanto Technology Llc Plants and seeds of spring canola variety SCV354718
EP2294198B1 (en) * 2008-05-28 2016-01-13 Insight Genomics Limited Methods and compositions for plant improvement
AU2009255855B2 (en) * 2008-06-03 2014-02-27 Insight Genomics Limited Compositions and methods for improving plants
CA2726990C (en) 2008-06-04 2020-09-08 Cellular Dynamics International, Inc. Methods for the production of ips cells using extra-chromosomal vectors
CN102216461B (zh) 2008-07-31 2015-05-13 英荷谷物有限公司 抗除草剂的向日葵植物
JP2012500005A (ja) 2008-08-12 2012-01-05 セルラー ダイナミクス インターナショナル， インコーポレイテッド ｉＰＳ細胞を生成するための方法
CA2734325A1 (en) 2008-08-18 2010-02-25 University Of Maryland, Baltimore Derivatives of apf and methods of use
WO2010042481A1 (en) 2008-10-06 2010-04-15 University Of Chicago Compositions and methods related to bacterial eap, emp, and/or adsa proteins
WO2010045324A1 (en) 2008-10-14 2010-04-22 Monsanto Technology Llc Utilization of fatty acid desaturases from hemiselmis spp.
CA2741211A1 (en) * 2008-11-04 2010-05-14 Dow Agrosciences Llc Omega-9 quality brassica juncea
CN102271704A (zh) 2008-11-28 2011-12-07 梅里亚有限公司 重组禽流感疫苗及其用途
WO2010064934A1 (en) 2008-12-01 2010-06-10 Vialactia Biosciences (Nz) Limited Methods and compositions for the improvement of plant tolerance to environmental stresses
US8431775B2 (en) 2008-12-04 2013-04-30 Pioneer Hi Bred International Inc Methods and compositions for enhanced yield by targeted expression of knotted1
EP2370568B1 (en) 2008-12-10 2017-07-19 Dana-Farber Cancer Institute, Inc. Mek mutations conferring resistance to mek inhibitors
JP2012515532A (ja) 2009-01-20 2012-07-12 ラモット アット テル アビブ ユニバーシティ， リミテッド Ｍｉｒ−２１プロモーター駆動性標的がん治療
US8373025B2 (en) 2009-02-09 2013-02-12 Chromatin Germplasm, Llc Herbicide resistant sorghum
US20120058523A1 (en) 2009-02-17 2012-03-08 Edenspace Systems Corporation Tempering of cellulosic biomass
MY189620A (en) 2009-02-20 2022-02-21 Malaysian Palm Oil Board A constitutive promoter from oil palm
WO2010114395A1 (en) 2009-04-01 2010-10-07 Vialactia Biosciences (Nz) Limited Control of gene expression in plants using a perennial ryegrass (lolium perenne l.) derived promoter
EP3281947B1 (en) 2009-04-03 2020-02-12 The University of Chicago Compositions and methods related to protein a (spa) variants
US8304616B2 (en) * 2009-04-07 2012-11-06 University Of Georgia Research Foundation, Inc. Soybean variety G00-3209
JP2012523850A (ja) 2009-04-20 2012-10-11 モンサント テクノロジー エルエルシー 植物中の複数ウイルス抵抗性
DK2424885T3 (en) 2009-04-28 2016-06-06 Univ Vanderbilt Compositions and methods for the treatment of disorders involving epitelcelleapoptose
US8779239B2 (en) 2009-05-04 2014-07-15 Pioneeri Hi-Bred International, Inc. Yield enhancement in plants by modulation of AP2 transcription factor
MY186558A (en) * 2009-05-13 2021-07-27 Malaysian Palm Oil Board A constitutive promoter from oil palm type 2
US20100299773A1 (en) * 2009-05-20 2010-11-25 Monsanto Technology Llc Methods and compositions for selecting an improved plant
US20120220015A1 (en) 2009-05-26 2012-08-30 Biolex Therapeutics Compositions and methods for production of aglycosylated plasminogen
WO2010138971A1 (en) 2009-05-29 2010-12-02 Edenspace Systems Corporation Plant gene regulatory elements
CA2952805C (en) 2009-06-05 2021-06-01 Cellular Dynamics International, Inc. Reprogramming t cells and hematopoietic cells
CA2765034A1 (en) 2009-06-09 2010-12-23 Pioneer Hi-Bred International, Inc. Early endosperm promoter and methods of use
US8071848B2 (en) * 2009-06-17 2011-12-06 Monsanto Technology Llc Plants and seeds of spring canola variety SCV218328
US8569582B2 (en) * 2009-06-22 2013-10-29 The Samuel Roberts Noble Foundation Method for transformation of grasses
CA2766075A1 (en) 2009-06-25 2010-12-29 Syngenta Participations Ag Methods for agrobacterium-mediated transformation of sugar cane
US20120131701A1 (en) 2009-08-05 2012-05-24 Chromocell Corporation Improved plants, microbes, and organisms
MX2012002189A (es) 2009-08-21 2012-03-16 Beeologics Inc Prevencion y cura de las enfermedades de los insectos beneficiosos a traves de moleculas.
EP2470653A1 (en) 2009-08-26 2012-07-04 Research Development Foundation Methods for creating antibody libraries
CA3184328A1 (en) 2009-08-28 2011-03-03 E. I. Du Pont De Nemours And Company Compositions and methods to control insect pests
ES2671570T3 (es) 2009-09-14 2018-06-07 Sillajen Biotherapeutics, Inc. Terapia combinada contra el cáncer con virus oncolítico de vaccinia
US9181559B2 (en) 2009-09-15 2015-11-10 Metabolix, Inc. Generation of high polyhydroxybutyrate producing oilseeds
WO2011050271A1 (en) 2009-10-23 2011-04-28 Monsanto Technology Llc Methods and compositions for expression of transgenes in plants
CN102597244B (zh) 2009-10-26 2014-07-23 先锋国际良种公司 胚珠体细胞特异性启动子及应用方法
WO2011070440A2 (en) 2009-12-10 2011-06-16 Ottawa Hospital Research Institute Oncolytic rhabdovirus
AR079767A1 (es) 2009-12-28 2012-02-15 Merial Ltd Antigeno ndv (virus de la enfermedad de newcastle) recombinante y usos del mismo
WO2011082318A2 (en) 2009-12-30 2011-07-07 Pioneer Hi-Bred International, Inc. Methods and compositions for the introduction and regulated expression of genes in plants
US8704041B2 (en) 2009-12-30 2014-04-22 Pioneer Hi Bred International Inc Methods and compositions for targeted polynucleotide modification
US9970021B2 (en) 2010-01-26 2018-05-15 Pioneer Hi-Bred International, Inc. HPPD-inhibitor herbicide tolerance
WO2011100508A2 (en) 2010-02-12 2011-08-18 Arizona Board Of Regents For And On Behalf Of Arizona State University Methods and compositions related to glycoprotein-immunoglobulin fusions
EP3028699B1 (en) 2010-02-25 2018-03-21 Dana-Farber Cancer Institute, Inc. Braf mutations conferring resistance to braf inhibitors
US8143488B2 (en) * 2010-02-26 2012-03-27 Monsanto Technoloy LLC Plants and seeds of spring canola variety SCV470336
US8138394B2 (en) * 2010-02-26 2012-03-20 Monsanto Technology Llc Plants and seeds of spring canola variety SCV431158
US8148611B2 (en) * 2010-02-26 2012-04-03 Monsanto Technology Llc Plants and seeds of spring canola variety SCV453784
EP3214174B1 (en) 2010-03-04 2019-10-16 InteRNA Technologies B.V. A mirna molecule defined by its source and its diagnostic and therapeutic uses in diseases or conditions associated with emt
BR112012022218A2 (pt) 2010-03-04 2018-07-10 Mendel Biotechnology Inc reguladores de transcrição para melhorar desempenho de planta
US8581048B2 (en) * 2010-03-09 2013-11-12 Monsanto Technology, Llc Plants and seeds of spring canola variety SCV119103
US8153865B2 (en) * 2010-03-11 2012-04-10 Monsanto Technology Llc Plants and seeds of spring canola variety SCV152154
NZ602278A (en) 2010-03-12 2014-12-24 Biolex Therapeutics Inc Bluetongue virus recombinant vaccines and uses thereof
EP2555794A4 (en) 2010-04-05 2014-01-15 Univ Chicago COMPOSITIONS AND METHODS RELATING TO PROTEIN A (SPA) ANTIBODIES AS IMMUNE REACTION AMPLIFIERS
US9249195B2 (en) 2010-04-07 2016-02-02 Vanderbilt University Reovirus vaccines and methods of use therefor
WO2011133512A1 (en) 2010-04-19 2011-10-27 Research Development Foundation Rtef-1 variants and uses thereof
AU2011242727B2 (en) 2010-04-20 2015-09-10 Seminis Vegetable Seeds, Inc. White-stem trait containing plants
US20110287936A1 (en) 2010-04-23 2011-11-24 E.I. Dupont De Nemours And Company Gene switch compositions and methods of use
MX2012012672A (es) 2010-05-06 2012-12-17 Du Pont Gen y proteina acc sintasa 3 de maiz y sus usos.
WO2011146754A1 (en) 2010-05-19 2011-11-24 The Samuel Roberts Noble Foundation, Inc. Altered leaf morphology and enhanced agronomic properties in plants
PT2580322T (pt) 2010-06-09 2018-03-01 Dana Farber Cancer Inst Inc Uma mutação em mek1 que confere resistência aos inibidores de raf e mek
CA2802087A1 (en) 2010-06-15 2011-12-22 Cellular Dynamics International, Inc. A compendium of ready-built stem cell models for interrogation of biological response
WO2011159684A2 (en) 2010-06-15 2011-12-22 Cellular Dynamics International, Inc. Generation of induced pluripotent stem cells from small volumes of peripheral blood
FR2961375B1 (fr) 2010-06-16 2016-05-13 Inst De Rech Pour Le Dev (Ird) Surproduction d'acide jasmonique dans des plantes transgeniques
WO2011163292A1 (en) 2010-06-24 2011-12-29 Syngenta Participations Ag Methods for agrobacterium-mediated transformation of sugar cane
EP4449858A3 (en) 2010-06-25 2025-04-16 Corteva Agriscience LLC Compositions and methods for enhancing resistance to northern leaf blight in maize
ES2655701T3 (es) 2010-07-02 2018-02-21 The University Of Chicago Composiciones y métodos relacionados con variantes de la proteína A (SpA)
NZ605420A (en) 2010-07-06 2015-02-27 Interna Technologies Bv Mirna and its diagnostic and therapeutic uses in diseases or conditions associated with melanoma, or in diseases or conditions associated with activated braf pathway
WO2012006440A2 (en) 2010-07-07 2012-01-12 Cellular Dynamics International, Inc. Endothelial cell production by programming
US8765470B2 (en) 2010-08-04 2014-07-01 Cellular Dynamics International, Inc. Reprogramming immortalized B-cells to induced pluripotent stem cells
CA2805937A1 (en) 2010-08-13 2012-02-16 Pioneer Hi-Bred International, Inc. Chimeric promoters and methods of use
US8865967B2 (en) 2010-08-23 2014-10-21 Pioneer Hi Bred International Inc Defensin variants and methods of use
CA2807836A1 (en) 2010-09-01 2012-03-08 Pioneer Hi-Bred International, Inc. Vacuole targeting peptides and methods of use
JP5793194B2 (ja) 2010-09-09 2015-10-14 ザ・ユニバーシティ・オブ・シカゴＴｈｅ Ｕｎｉｖｅｒｓｉｔｙ Ｏｆ Ｃｈｉｃａｇｏ 感染防御ブドウ球菌抗原が関与する方法および組成物
BR112013005973A2 (pt) 2010-09-15 2019-09-24 Metabolix Inc aumento do fluxo de carbono para a produção de poli-hidroxibutirato em culturas de biomassa
WO2012040295A2 (en) 2010-09-22 2012-03-29 The Regents Of The University Of Colorado Therapeutic applications of smad7
WO2012061615A1 (en) 2010-11-03 2012-05-10 The Samuel Roberts Noble Foundation, Inc. Transcription factors for modification of lignin content in plants
WO2012074868A2 (en) 2010-12-03 2012-06-07 Ms Technologies, Llc Optimized expression of glyphosate resistance encoding nucleic acid molecules in plant cells
CA2821154A1 (en) 2010-12-22 2012-06-28 Pioneer Hi-Bred International, Inc. Viral promoter, truncations thereof, and methods of use
BR112013014698A2 (pt) 2010-12-22 2017-03-07 E I Du Point De Nemours & Company constructo de dna, vetor, célula vegetal, planta, semente transgênica proveniente da planta, método para expressão de uma sequência de nucleotídeos em uma planta, método para expressão de uma sequência de nucleotídeos em uma célula vegetal, método para expressar seletivamente uma sequência de nucleotídeos em tecidos de raiz, caule, grão e pendão de milho
US8822762B2 (en) 2010-12-28 2014-09-02 Pioneer Hi Bred International Inc Bacillus thuringiensis gene with lepidopteran activity
UY33854A (es) 2010-12-30 2012-07-31 Dow Agrosciences Llc Moléculas de ácido nucleico que se dirigen a la subunidad c de la atpasa vacuolar y confieren resistencia a las plagas de coleópteros
BRPI1107329A2 (pt) 2010-12-30 2019-11-19 Dow Agrosciences Llc moléculas de ácido nucléico que direcionadas à subunidade h de atpase vacuolar que conferem resistência a pragas de coleópteros, vetor de transformação de planta, célula transformada, bem como métodos para controlar uma população de praga de coleóptero, controlar uma infestação pela dita praga, melhorar o rendimento de uma safra e para produzir uma célula transgênica
AP2013007016A0 (en) 2010-12-30 2013-07-31 Dow Agrosciences Llc Nucleic acid molecules that confer resistance to colepteran pests
CN103429258B (zh) 2011-01-04 2016-03-09 新罗杰公司 通过施用溶瘤痘苗病毒生成针对肿瘤抗原的抗体和生成肿瘤特异性补体依赖性细胞毒性
EP2474617A1 (en) 2011-01-11 2012-07-11 InteRNA Technologies BV Mir for treating neo-angiogenesis
AR084844A1 (es) 2011-01-14 2013-06-26 Jones Jeffrey B Arboles de citricos con resistencia a cancro de los citricos
MX347631B (es) 2011-02-01 2017-05-04 Colorado Wheat Res Foundation Inc Plantas resistentes a herbicida en acetil co-enzima a carboxilasa.
AU2012214643B2 (en) 2011-02-07 2016-12-15 Research Development Foundation Engineered immunoglobulin Fc polypeptides
WO2012109208A2 (en) 2011-02-08 2012-08-16 Cellular Dynamics International, Inc. Hematopoietic precursor cell production by programming
US9109231B2 (en) 2011-02-11 2015-08-18 Pioneer Hi Bred International Inc Synthetic insecticidal proteins active against corn rootworm
US8916377B2 (en) 2011-02-15 2014-12-23 Pioneer Hi Bred International Inc Root-preferred promoter and methods of use
US8878007B2 (en) 2011-03-10 2014-11-04 Pioneer Hi Bred International Inc Bacillus thuringiensis gene with lepidopteran activity
EP2689019A2 (en) 2011-03-23 2014-01-29 Pioneer Hi-Bred International Inc. Methods for producing a complex transgenic trait locus
WO2012131495A2 (en) 2011-03-30 2012-10-04 Universidad Nacional Autónoma de México Mutant bacillus thuringiensis cry genes and methods of use
US8513487B2 (en) 2011-04-07 2013-08-20 Zenon LISIECZKO Plants and seeds of spring canola variety ND-662c
US8513494B2 (en) 2011-04-08 2013-08-20 Chunren Wu Plants and seeds of spring canola variety SCV695971
WO2012136653A1 (en) 2011-04-08 2012-10-11 Novvac Aps Proteins and nucleic acids useful in vaccines targeting staphylococcus aureus
WO2012142106A1 (en) 2011-04-11 2012-10-18 Targeted Growth, Inc. Identification and the use of krp mutants in plants
BR112013026600A2 (pt) 2011-04-15 2016-12-27 Pioneer Hi Bred Int método para a produção de uma planta híbrida autorreprodutora, planta híbrida autorreprodutora, semente, cassete de supressão, planta e cassete de expressão
US8507761B2 (en) 2011-05-05 2013-08-13 Teresa Huskowska Plants and seeds of spring canola variety SCV372145
US8901371B2 (en) 2011-05-06 2014-12-02 The Samuel Roberts Noble Foundation, Inc. Compositions and methods for improved plant feedstock
US8945588B2 (en) 2011-05-06 2015-02-03 The University Of Chicago Methods and compositions involving protective staphylococcal antigens, such as EBH polypeptides
US9062317B2 (en) 2011-05-09 2015-06-23 E I Du Pont De Nemours And Company Methods and compositions for silencing gene families using artificial microRNAs
US8513495B2 (en) 2011-05-10 2013-08-20 Dale Burns Plants and seeds of spring canola variety SCV291489
US8735662B2 (en) 2011-05-16 2014-05-27 Nunhems B.V. Lettuce variety salmon
US9150625B2 (en) 2011-05-23 2015-10-06 E I Du Pont De Nemours And Company Chloroplast transit peptides and methods of their use
US9364532B2 (en) 2011-06-08 2016-06-14 Children's Hospital Of Eastern Ontario Research Institute Inc. Compositions and methods for glioblastoma treatment
US20140216118A1 (en) 2011-06-14 2014-08-07 Synthon Biopharmaceuticals B.V. Compositions and Methods for Making and Biocontaining Auxotrophic Transgenic Plants
CN102329816B (zh) * 2011-06-15 2012-12-26 北京未名凯拓作物设计中心有限公司 一种农杆菌介导的纵切幼苗上胚轴顶端转化大豆的方法
AR086995A1 (es) 2011-06-21 2014-02-05 Pioneer Hi Bred Int Metodos y composiciones para producir plantas con esterilidad masculina
WO2013009825A1 (en) 2011-07-11 2013-01-17 Cellular Dynamics International, Inc. Methods for cell reprogramming and genome engineering
US20130097734A1 (en) 2011-07-12 2013-04-18 Two Blades Foundation Late blight resistance genes
US9951346B2 (en) 2011-08-03 2018-04-24 Pioneer Hi-Bred International, Inc. Methods and compositions for targeted integration in a plant
US9556281B2 (en) 2011-08-15 2017-01-31 The University Of Chicago Compositions and methods related to antibodies to staphylococcal protein A
US20130101664A1 (en) 2011-08-18 2013-04-25 Donald W. Kufe Muc1 ligand traps for use in treating cancers
US11377663B1 (en) 2011-08-30 2022-07-05 Monsanto Technology Llc Genetic regulatory elements
US12188028B1 (en) 2011-08-30 2025-01-07 Monsanto Technology, Llc Methods for making genetic regulatory elements
AU2012301912A1 (en) 2011-08-31 2014-03-06 E. I. Dupont De Nemours & Company Methods for tissue culture and transformation of sugarcane
EP2766388A1 (en) 2011-10-12 2014-08-20 Møller, Niels Iversen Peptides derived from campylobacter jejuni and their use in vaccination
US20130111634A1 (en) 2011-10-28 2013-05-02 E I Du Pont De Nemours And Company And Pioneer Hi-Bred International Methods and compositions for silencing genes using artificial micrornas
WO2013063344A1 (en) 2011-10-28 2013-05-02 Pioneer Hi-Bred International, Inc. Engineered pep carboxylase variants for improved plant productivity
US20140182011A1 (en) 2011-11-03 2014-06-26 The University Of Hong Kong Methods Using Acyl-Coenzyme A-Binding Proteins to Enchance Drought Tolerance in Genetically Modified Plants
FR2984076A1 (fr) 2011-12-15 2013-06-21 Inst Rech Developpement Ird Surproduction de jasmonates dans des plantes transgeniques
ES2886147T3 (es) 2011-12-22 2021-12-16 Interna Tech B V MiARN para el tratamiento del cáncer de cabeza y de cuello
US20130180008A1 (en) 2012-01-06 2013-07-11 Pioneer Hi Bred International Inc Ovule Specific Promoter and Methods of Use
US9006515B2 (en) 2012-01-06 2015-04-14 Pioneer Hi Bred International Inc Pollen preferred promoters and methods of use
CA2860692A1 (en) 2012-01-06 2013-07-11 Pioneer Hi-Bred International, Inc. A method to screen plants for genetic elements inducing parthenogenesis in plants
CA2860611A1 (en) 2012-01-06 2013-07-11 Pioneer Hi-Bred International, Inc. Compositions and methods for the expression of a sequence in a reproductive tissue of a plant
EP2807258B1 (en) 2012-01-23 2017-04-26 E. I. du Pont de Nemours and Company Down-regulation of gene expression using artificial micrornas for silencing fatty acid biosynthestic genes
BR112014018294B1 (pt) 2012-01-26 2022-01-11 Norfolk Plant Sciences, Ltd Método para produzir uma planta, cassete de expressão, e, célula bacteriana
AR089793A1 (es) 2012-01-27 2014-09-17 Du Pont Metodos y composiciones para generar locus de rasgos transgenicos complejos
EP2809144B1 (en) 2012-02-01 2019-11-13 Dow AgroSciences LLC Novel class of glyphosate resistance genes
JP6208692B2 (ja) 2012-02-02 2017-10-04 ダウ アグロサイエンシィズ エルエルシー 植物トランス活性化相互作用モチーフおよびその使用
AU2013205557B2 (en) 2012-04-17 2016-04-21 Corteva Agriscience Llc Synthetic brassica-derived chloroplast transit peptides
CA2871008C (en) 2012-04-23 2022-11-22 Bayer Cropscience Nv Targeted genome engineering in plants
US8802935B2 (en) 2012-04-26 2014-08-12 Monsanto Technology Llc Plants and seeds of spring canola variety SCV942568
NZ702282A (en) 2012-04-26 2016-07-29 Univ Chicago Compositions and methods related to antibodies that neutralize coagulase activity during staphylococcus aureus disease
US8835720B2 (en) 2012-04-26 2014-09-16 Monsanto Technology Llc Plants and seeds of spring canola variety SCV967592
HRP20201127T1 (hr) 2012-04-26 2020-11-27 University Of Chicago Antigeni stafilokokne koagulaze i metode primjene istih
US8878009B2 (en) 2012-04-26 2014-11-04 Monsanto Technology, LLP Plants and seeds of spring canola variety SCV318181
US8859857B2 (en) 2012-04-26 2014-10-14 Monsanto Technology Llc Plants and seeds of spring canola variety SCV259778
BR112014027468A2 (pt) 2012-05-04 2017-06-27 Du Pont polinucleotídeo isolado ou recombinante, construção de dna recombinante, célula, planta, explante vegetal, semente transgênica, polipeptídeo isolado, composição, métodos de produção de meganuclease, de introdução de rompimento e de integração de um polinucleotídeo.
JP6559063B2 (ja) 2012-05-07 2019-08-14 サンガモ セラピューティクス， インコーポレイテッド 導入遺伝子のヌクレアーゼ媒介標的化組み込みのための方法および組成物
US10045499B2 (en) 2012-05-24 2018-08-14 Iowa State University Research Foundation, Inc. Arabidopsis nonhost resistance gene(s) and use thereof to engineer disease resistant plants
WO2013180809A1 (en) 2012-05-29 2013-12-05 North Carolina Central University Methods for the production of cytoprotective asialo-erythropoietin in plants and its purification from plant tissues
US9347105B2 (en) 2012-06-15 2016-05-24 Pioneer Hi Bred International Inc Genetic loci associated with resistance of soybean to cyst nematode and methods of use
CA2876426A1 (en) 2012-06-15 2013-12-19 E. I. Du Pont De Nemours And Company Methods and compositions involving als variants with native substrate preference
CN102754597B (zh) * 2012-06-21 2013-12-04 华南农业大学 一种诱导大豆下胚轴外植体的不定芽再生的方法
NZ629197A (en) 2012-06-26 2016-07-29 Forage Genetics Internat Llc Methods and composition for enhanced forage quality
US9587247B2 (en) 2012-08-16 2017-03-07 Wisconsin Alumni Research Foundation Plants with altered phytochromes
WO2014036048A1 (en) 2012-08-30 2014-03-06 E. I. Du Pont De Nemours And Company Long intergenic non-coding rnas in maize
UA118090C2 (uk) 2012-09-07 2018-11-26 ДАУ АГРОСАЙЄНСІЗ ЕлЕлСі Спосіб інтегрування послідовності нуклеїнової кислоти, що представляє інтерес, у ген fad2 у клітині сої та специфічний для локусу fad2 білок, що зв'язується, здатний індукувати спрямований розрив
HK1217732A1 (zh) 2012-09-07 2017-01-20 美国陶氏益农公司 Fad3性能基因座及相應的能夠誘導靶向斷裂的靶位點特異性結合蛋白
AU2013224677B2 (en) 2012-09-13 2017-05-11 Plant Bioscience Limited Brassica oleracea plants with improved nutritional value
AU2013224684B2 (en) 2012-09-13 2017-07-06 Plant Bioscience Limited GENETIC MARKERS FOR Myb28
EP2895610B1 (en) 2012-09-13 2019-11-06 Indiana University Research and Technology Corporation Compositions and systems for conferring disease resistance in plants and methods of use thereof
FI3622810T3 (fi) 2012-09-24 2024-03-21 Seminis Vegetable Seeds Inc Menetelmät ja koostumukset kasvituotteiden säilyvyyden pidentämiseksi
WO2014059155A1 (en) 2012-10-11 2014-04-17 Pioneer Hi-Bred International, Inc. Guard cell promoters and uses thereof
US9783820B2 (en) 2012-10-15 2017-10-10 Pioneer Hi-Bred International, Inc. Methods and compositions to enhance activity of Cry endotoxins
US9890216B2 (en) 2012-10-23 2018-02-13 Board Of Regents, The University Of Texas System Antibodies with engineered IgG Fc domains
WO2014068439A2 (en) 2012-10-30 2014-05-08 Agresearch Limited Improved acyltransferase polynucleotides, polypeptides, and methods of use
MX359167B (es) 2012-10-30 2018-09-18 Agresearch Ltd Polinucleotidos, polipeptidos y metodos de uso novedosos de aciltransferasa.
US11312971B2 (en) 2012-10-30 2022-04-26 Agresearch Limited Enhanced acyltransferase polynucleotides, polypeptides and methods of use
WO2014072357A1 (en) 2012-11-06 2014-05-15 Interna Technologies B.V. Combination for use in treating diseases or conditions associated with melanoma, or treating diseases or conditions associated with activated b-raf pathway
CN104902748A (zh) 2012-11-23 2015-09-09 河西马有限公司 抗病原体方法
US20140173775A1 (en) 2012-12-13 2014-06-19 Pioneer Hi-Bred International, Inc. Methods and compositions for producing and selecting transgenic plants
AU2013361220A1 (en) 2012-12-21 2015-04-02 Pioneer Hi-Bred International, Inc. Compositions and methods for auxin-analog conjugation
AU2013365731B2 (en) 2012-12-21 2018-11-22 The New Zealand Institute For Plant And Food Research Limited Regulation of gene expression
EP3919505B1 (en) 2013-01-16 2023-08-30 Emory University Uses of cas9-nucleic acid complexes
US10125373B2 (en) 2013-01-22 2018-11-13 Arizona Board Of Regents On Behalf Of Arizona State University Geminiviral vector for expression of rituximab
JP2016513115A (ja) 2013-02-21 2016-05-12 チルドレンズ ホスピタル オブ イースタン オンタリオ リサーチ インスティチュート インコーポレイテッド ワクチン組成物
CN105229144A (zh) 2013-02-22 2016-01-06 细胞动力学国际有限公司 通过组合的遗传工程和化学工程经由正向编程产生肝细胞
NL1040073C2 (en) 2013-02-28 2014-09-01 Agrisemen B V Lactuca sativa with bremia lactucae (downy mildew) resistance.
WO2014134144A1 (en) 2013-02-28 2014-09-04 The General Hospital Corporation Mirna profiling compositions and methods of use
US20160010094A1 (en) 2013-03-01 2016-01-14 University Of Pretoria Transgenic cell selection
CN105189760A (zh) 2013-03-01 2015-12-23 加利福尼亚大学董事会 用于将rna聚合酶和非编码rna生物发生靶向特定基因座的方法和组合物
CA2904329C (en) 2013-03-08 2021-11-30 The Regents Of The University Of Colorado, A Body Corporate Ptd-smad7 therapeutics
US20160326540A1 (en) 2013-03-11 2016-11-10 Pioneer Hi-Bred International, Inc. Methods and Compositions Employing a Sulfonylurea-Dependent Stabilization Domain
CA2905377A1 (en) 2013-03-11 2014-10-09 Pioneer Hi-Bred International, Inc. Methods and compositions to improve the spread of chemical signals in plants
US9273322B2 (en) 2013-03-12 2016-03-01 Pioneer Hi Bred International Inc Root-preferred promoter and methods of use
UA123532C2 (uk) 2013-03-12 2021-04-21 Е. І. Дю Пон Де Немур Енд Компані Спосіб ідентифікації варіантного сайта розпізнавання для сконструйованого засобу, що рідко розщеплює, для індукції двониткового розриву
US9243258B2 (en) 2013-03-12 2016-01-26 Pioneer Hi Bred International Inc Root-preferred promoter and methods of use
US9803214B2 (en) 2013-03-12 2017-10-31 Pioneer Hi-Bred International, Inc. Breeding pair of wheat plants comprising an MS45 promoter inverted repeat that confers male sterility and a construct that restores fertility
US9416368B2 (en) 2013-03-13 2016-08-16 E I Du Pont De Nemours And Company Identification of P. pachyrhizi protein effectors and their use in producing Asian soybean rust (ASR) resistant plants
BR112015023268A2 (pt) 2013-03-13 2018-05-02 Du Pont método para reduzir a expressão, construto de dna, método de obtenção de célula vegetal, método de obtenção de planta transgênica ou uma parte de planta
BR112015023285B1 (pt) 2013-03-14 2022-03-03 Pioneer Hi-Bred International, Inc. Cassete de expressão, célula hospedeira bacteriana e método para controlar uma praga de planta do tipo coleóptero
BR112015023272A2 (pt) 2013-03-14 2017-07-18 Pioneer Hi Bred Int célula vegetal, planta, explante vegetal, semente transgênica, método para produzir uma célula vegetal tendo um polinucleotídeo heterólogo que codifica um polipeptídeo tendo atividade de dicamba descarboxilase, método para controlar plantas daninhas em um campo contendo uma cultura e método para controlar plantas daninhas em um campo contendo uma cultura
EP2970935A1 (en) 2013-03-14 2016-01-20 Pioneer Hi-Bred International, Inc. Compositions having dicamba decarboxylase activity and methods of use
CA2901316A1 (en) 2013-03-15 2014-09-25 Pioneer Hi-Bred International, Inc. Phi-4 polypeptides and methods for their use
EP2986725A4 (en) 2013-04-19 2016-11-09 Agres Ltd Methods and materials for encapsulating proteins
EP2810952A1 (en) 2013-06-03 2014-12-10 Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V. Novel pest control methods
WO2015006105A1 (en) 2013-07-09 2015-01-15 Board Of Trustees Of Michigan State University Transgenic plants produced with a k-domain, and methods and expression cassettes related thereto
US11459579B2 (en) 2013-07-09 2022-10-04 Board Of Trustees Of Michigan State University Transgenic plants produced with a K-domain, and methods and expression cassettes related thereto
BR122022020536B1 (pt) 2013-08-08 2023-11-21 Pioneer Hi-Bred International, Inc. Molécula de ácido nucleico isolada, construto de dna, célula hospedeira bacteriana, polipeptídeo isolado, composição, método para controlar uma população, método para matar uma praga, método para produzir um polipeptídeo, método para produzir uma planta ou célula vegetal, método para proteger uma planta
MX359026B (es) 2013-08-16 2018-09-12 Pioneer Hi Bred Int Proteinas insecticidas y metodos de uso.
CA3221516A1 (en) 2013-08-22 2015-02-26 E. I. Du Pont De Nemours And Company Plant genome modification using guide rna/cas endonuclease systems and methods of use
US11819555B2 (en) 2013-09-09 2023-11-21 Figene, Llc Gene therapy for the regeneration of chondrocytes or cartilage type cells
EA201690560A1 (ru) 2013-09-11 2016-10-31 Пайонир Хай-Бред Интернэшнл, Инк. Регуляторные элементы растений и способы их применения
US10667524B2 (en) 2013-09-13 2020-06-02 Pioneer Hi-Bred International, Inc. Insecticidal proteins and methods for their use
AU2014334590A1 (en) 2013-10-18 2016-04-28 Pioneer Hi-Bred International, Inc. Glyphosate-N-acetyltransferase (GLYAT) sequences and methods of use
US20160249542A1 (en) 2013-10-29 2016-09-01 Pioneer Hi-Bred International Inc. Self-reproducing hybrid plants
PT3552482T (pt) 2013-10-29 2022-09-27 Biotech Inst Llc Reprodução, produção, transformação e uso de canábis especial
MX358066B (es) 2013-11-04 2018-08-03 Dow Agrosciences Llc Óptimos loci de soya.
BR102014027442B1 (pt) 2013-11-04 2022-09-27 Dow Agrosciences Llc Molécula de ácido nucleico recombinante, uso de uma planta de milho, parte de planta de milho ou célula de planta de milho compreendendo a mesma e método para produzir uma célula vegetal transgênica compreendendo um dna de interesse
KR102269769B1 (ko) 2013-11-04 2021-06-28 코르테바 애그리사이언스 엘엘씨 최적 메이즈 유전자좌
EP3065775B1 (en) 2013-11-08 2020-09-30 The Board of Regents of the University of Texas System Vh4 antibodies against gray matter neuron and astrocyte
US10028902B2 (en) 2013-11-08 2018-07-24 Baylor Research Institute Nuclear localization of GLP-1 stimulates myocardial regeneration and reverses heart failure
TW201525136A (zh) 2013-11-26 2015-07-01 Dow Agrosciences Llc 利用破囊壺菌ＰＵＦＡ合成酶於油籽作物中生成ω－３長鏈多不飽和脂肪酸
WO2015082536A1 (en) 2013-12-03 2015-06-11 Evaxion Biotech Aps Proteins and nucleic acids useful in vaccines targeting staphylococcus aureus
BR102014031844A2 (pt) 2013-12-20 2015-10-06 Dow Agrosciences Llc ras oposto (rop) e moléculas de ácido nucleico relacionadas que conferem resistência a pragas de coleópteros e hemípteros
WO2015095750A1 (en) 2013-12-20 2015-06-25 Dow Agrosciences Llc Rnapii-140 nucleic acid molecules that confer resistance to coleopteran pests
BR102015000943A2 (pt) 2014-01-17 2016-06-07 Dow Agrosciences Llc expressão aumentada de proteína em planta
MX380864B (es) 2014-01-29 2025-03-12 Dana Farber Cancer Inst Inc Anticuerpos contra el dominio extracelular de muc1-c (muc1-c/ecd).
US20170166920A1 (en) 2014-01-30 2017-06-15 Two Blades Foundation Plants with enhanced resistance to phytophthora
WO2015120270A1 (en) 2014-02-07 2015-08-13 Pioneer Hi Bred International, Inc. Insecticidal proteins and methods for their use
UA120608C2 (uk) 2014-02-07 2020-01-10 Піонір Хай-Бред Інтернешнл, Інк. Очищений поліпептид ptip-83 та спосіб його застосування
US10214741B2 (en) 2014-02-14 2019-02-26 University Of Utah Research Foundation Methods and compositions for inhibiting retinopathy of prematurity
EP3110836A1 (en) 2014-02-25 2017-01-04 Research Development Foundation Sty peptides for inhibition of angiogenesis
EP3971283A1 (en) 2014-02-27 2022-03-23 Monsanto Technology LLC Compositions and methods for site directed genomic modification
US20150307565A1 (en) 2014-04-02 2015-10-29 Wisconsin Alumni Research Foundation Strategy to engineer phytochromes with improved action in crop fields
WO2015150465A2 (en) 2014-04-03 2015-10-08 Basf Se Plants having increased tolerance to herbicides
WO2015164228A1 (en) 2014-04-21 2015-10-29 Cellular Dynamics International, Inc. Hepatocyte production via forward programming by combined genetic and chemical engineering
WO2015171603A1 (en) 2014-05-06 2015-11-12 Two Blades Foundation Methods for producing plants with enhanced resistance to oomycete pathogens
JP2017514494A (ja) 2014-05-06 2017-06-08 ダウ アグロサイエンシィズ エルエルシー 種子準備のためのシステムおよび使用の方法
JP2017521747A (ja) 2014-05-06 2017-08-03 ダウ アグロサイエンシィズ エルエルシー 種子を撮像および配向するためのシステムならびに使用の方法
UY36113A (es) 2014-05-07 2015-10-30 Dow Agrosciences Llc Moléculas de ácido nucleico dre4 que confieren resistencia a plagas de coleópteros
EP3166965B1 (en) 2014-07-10 2020-09-09 Benson Hill, Inc. Compositions and methods for increasing plant growth and yield
CN106687594A (zh) 2014-07-11 2017-05-17 纳幕尔杜邦公司 用于产生对草甘膦除草剂具有抗性的植物的组合物和方法
EP3174983A4 (en) 2014-08-01 2017-12-13 The New Zealand Institute for Plant and Food Research Limited Methods and materials for producing coreless fruit
US20170218384A1 (en) 2014-08-08 2017-08-03 Pioneer Hi-Bred International, Inc. Ubiquitin promoters and introns and methods of use
BR112017003757A2 (pt) 2014-09-12 2017-12-26 Du Pont ?plantas de milho, partes de planta de milho ou sementes de milho?
CA2961733A1 (en) 2014-09-17 2016-03-24 Pioneer Hi-Bred International, Inc. Compositions and methods to control insect pests
CN113016623A (zh) 2014-09-26 2021-06-25 谱赛科美国股份有限公司 用于甜菊的单核苷酸多态性(snp)标志物
WO2016046288A1 (en) 2014-09-26 2016-03-31 Philip Morris Products S.A Reducing tobacco specific nitrosamines through alteration of the nitrate assimilation pathway
BR102014023930A2 (pt) 2014-09-26 2016-05-17 Ctc Ct De Tecnologia Canavieira S A método para transformar uma célula de planta ou tecido de planta usando agrobacterium, planta transgênica, célula transgênica ou tecido transgênico, meio de cultura, e, uso de um método para transformar uma célula de planta ou tecido de planta
EP4696781A3 (en) 2014-09-26 2026-04-01 Pioneer Hi-Bred International, Inc. Wheat ms1 polynucleotides, polypeptides, and methods of use
CN113652416A (zh) 2014-10-06 2021-11-16 奥驰亚客户服务有限公司 烟草植物中腋芽生长的遗传控制
CN107108705B (zh) 2014-10-16 2021-05-25 先锋国际良种公司 杀昆虫蛋白及其使用方法
RU2750459C2 (ru) 2014-10-16 2021-06-28 Пайонир Хай-Бред Интернэшнл, Инк. Инсектицидные полипептиды с широким спектром активности и их применения
WO2016061197A1 (en) 2014-10-16 2016-04-21 Pioneer Hi-Bred International, Inc. Insecticidal polypeptides having improved activity spectrum and uses thereof
RU2727428C2 (ru) 2014-11-04 2020-07-21 Агрисерч Лимитед Способы усовершенствования однодольных растений
CN107205355B (zh) 2014-11-04 2021-04-30 农牧研究公司 用于植物改良的方法
EP3224371B1 (en) 2014-11-04 2024-10-09 Ball Horticultural Company Altered pigment deposition in tagetes patula
EP3218497A1 (en) 2014-11-12 2017-09-20 NMC Inc. Transgenic plants with engineered redox sensitive modulation of photosynthetic antenna complex pigments and methods for making the same
EP3218002B1 (en) 2014-11-13 2020-08-19 Evaxion Biotech ApS Peptides derived from acinetobacter baumannii and their use in vaccination
WO2016100309A1 (en) 2014-12-16 2016-06-23 Pioneer Hi-Bred International, Inc. Restoration of male fertility in wheat
WO2016100804A1 (en) 2014-12-19 2016-06-23 AgBiome, Inc. Methods and compositions for providing resistance to glufosinate
ES2811279T3 (es) 2014-12-22 2021-03-11 Fraunhofer Ges Forschung Moléculas de ácido nucleico de Nucampholin para controlar plagas de insectos coleópteros
US10434162B2 (en) 2015-01-12 2019-10-08 Evaxion Biotech Aps Proteins and nucleic acids useful in vaccines targeting Klebsiella pneumoniae
EP3244727B1 (en) 2015-01-15 2025-10-22 Pioneer Hi-Bred International, Inc. Insecticidal proteins and methods for their use
JP7040941B2 (ja) 2015-01-21 2022-03-23 ビーエーエスエフ ソシエタス・ヨーロピア 除草剤耐性の増加した植物
EP3250675A1 (en) 2015-01-28 2017-12-06 SABIC Global Technologies B.V. Methods and compositions for high-efficiency production of biofuel and/or biomass
WO2016130516A1 (en) 2015-02-09 2016-08-18 Research Development Foundation Engineered immunoglobulin fc polypeptides displaying improved complement activation
WO2016134293A1 (en) 2015-02-20 2016-08-25 Baylor College Of Medicine p63 INACTIVATION FOR THE TREATMENT OF HEART FAILURE
EP3262176A1 (en) 2015-02-25 2018-01-03 Pioneer Hi-Bred International, Inc. Composition and methods for regulated expression of a guide rna/cas endonuclease complex
CA2977026A1 (en) 2015-03-11 2016-09-15 E.I. Du Pont De Nemours And Company Insecticidal combinations of pip-72 and methods of use
EP3067424A1 (en) 2015-03-13 2016-09-14 Dow AgroSciences LLC Rna polymerase i1 nucleic acid molecules to control insect pests
WO2016149352A1 (en) 2015-03-19 2016-09-22 Pioneer Hi-Bred International Inc Methods and compositions for accelerated trait introgression
WO2016164810A1 (en) 2015-04-08 2016-10-13 Metabolix, Inc. Plants with enhanced yield and methods of construction
WO2016165729A1 (en) 2015-04-13 2016-10-20 Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V. Novel aflatoxin and fungal infection control methods
CA3285074A1 (en) 2015-04-28 2026-03-02 Monsanto Technology Llc Methods and compositions for producing brachytic corn plants
UA126326C2 (uk) 2015-04-30 2022-09-21 Монсанто Текнолоджі Елелсі Спосіб відбору рослини каноли, стійкої до кили капустяних
EP3091076A1 (en) 2015-05-07 2016-11-09 Limagrain Europe Polynucleotide responsible of haploid induction in maize plants and related processes
EP3294759B1 (en) 2015-05-08 2021-04-07 Benson Hill, Inc. Methods for increasing plant growth and yield by using an ictb sequence
EP3294892B1 (en) 2015-05-09 2020-11-25 Two Blades Foundation Late blight resistance gene from solanum americanum and methods of use
EP3294878A1 (en) 2015-05-15 2018-03-21 Pioneer Hi-Bred International, Inc. Guide rna/cas endonuclease systems
RU2017144238A (ru) 2015-05-19 2019-06-19 Пайонир Хай-Бред Интернэшнл, Инк. Инсектицидные белки и способы их применения
BR102016012010A2 (pt) 2015-05-29 2020-03-24 Dow Agrosciences Llc Molécula de ácido nucleico, de ácido ribonucleico (rna) e de ácido ribonucleico de filamento duplo (dsrna), usos de célula, planta e semente, produto primário, bem como métodos para controlar uma população de pragas coleópteras e/ou hemípteras, para melhorar o rendimento de uma cultura, e para produzir uma célula vegetal transgênica e uma planta transgênica
WO2016205445A1 (en) 2015-06-16 2016-12-22 Pioneer Hi-Bred International, Inc. Compositions and methods to control insect pests
AU2016279077A1 (en) 2015-06-18 2019-03-28 Omar O. Abudayyeh Novel CRISPR enzymes and systems
EP3436575A1 (en) 2015-06-18 2019-02-06 The Broad Institute Inc. Novel crispr enzymes and systems
US9790490B2 (en) 2015-06-18 2017-10-17 The Broad Institute Inc. CRISPR enzymes and systems
EP4218404A3 (en) 2015-06-26 2023-09-06 Altria Client Services LLC Compositions and methods for producing tobacco plants and products having altered alkaloid levels
EP3317295B1 (en) 2015-07-04 2022-05-18 Evaxion Biotech A/S Proteins and nucleic acids useful in vaccines targeting pseudomonas aeruginosa
CN109475096B (zh) 2015-08-06 2022-08-23 先锋国际良种公司 植物来源的杀昆虫蛋白及其使用方法
BR112018000541B1 (pt) 2015-08-18 2023-11-28 Monsanto Technology Llc Métodos para selecionar uma população de plantas ou sementes de algodão staygreen (stg), para selecionar uma planta ou semente de algodão e para avaliar uma coleção de germoplasma de algodão
WO2017040380A2 (en) 2015-08-28 2017-03-09 Research Development Foundation Engineered antibody fc variants
DK3341483T3 (da) 2015-08-28 2020-03-16 Pioneer Hi Bred Int Ochrobactrum-medieret transformation af planter
US10858709B2 (en) 2015-09-10 2020-12-08 Monsanto Technology Llc Methods for producing corn plants with downy mildew resistance and compositions thereof
EP3150626A1 (en) 2015-10-01 2017-04-05 University of Vienna Means and methods for accelerated breeding by inducing targeted stimulation of meiotic recombination
WO2017062790A1 (en) 2015-10-09 2017-04-13 Two Blades Foundation Cold shock protein receptors and methods of use
FI4144844T3 (fi) 2015-10-12 2025-11-24 Dupont Us Holding Llc Suojatut dna-templaatit geenimuokkaukseen ja homologisen rekombinaation lisäämiseen soluissa ja niiden käyttömenetelmät
EA037614B1 (ru) 2015-10-16 2021-04-21 Пайонир Хай-Бред Интернэшнл, Инк. Получение растений маиса с повышенной устойчивостью к северной пятнистости листьев
WO2021257206A1 (en) 2020-06-17 2021-12-23 Pioneer Hi-Bred International, Inc. Generating maize plants with enhanced resistance to northern leaf blight
ES2862676T3 (es) 2015-10-20 2021-10-07 Fujifilm Cellular Dynamics Inc Producción de células precursoras hematopoyéticas multilinaje mediante programación genética
CN108138155A (zh) 2015-10-20 2018-06-08 先锋国际良种公司 经由指导cas系统恢复非功能性基因产物的功能及使用方法
CN109153980B (zh) 2015-10-22 2023-04-14 布罗德研究所有限公司 Vi-b型crispr酶和系统
AR106931A1 (es) 2015-10-22 2018-03-07 Basf Se Plantas que tienen una mayor tolerancia a herbicidas
US12203089B2 (en) 2015-10-22 2025-01-21 Basf Se Plants having increased tolerance to herbicides
WO2017070458A2 (en) 2015-10-23 2017-04-27 Donald Danforth Plant Science Center Genes encoding c4 transporter proteins for use in increasing crop plant yield
MX2018005274A (es) 2015-10-30 2019-09-19 The Regents Of The Universtiy Of California Metodos para la generacion de celulas-t a partir de celulas madre y metodos inmunoterapeuticos que utilizan las celulas-t.
WO2017079202A1 (en) 2015-11-02 2017-05-11 Board Of Regents, The University Of Texas System Methods of cd40 activation and immune checkpoint blockade
US20180258438A1 (en) 2015-11-06 2018-09-13 Pioneer Hi-Bred International, Inc. Generation of complex trait loci in soybean and methods of use
WO2017078836A1 (en) 2015-11-06 2017-05-11 Pioneer Hi-Bred International, Inc. Methods and compositions of improved plant transformation
AU2016349632A1 (en) 2015-11-07 2018-05-24 Multivir Inc. Compositions comprising tumor suppressor gene therapy and immune checkpoint blockade for the treatment of cancer
JP6987068B2 (ja) 2015-11-09 2021-12-22 ザ・チルドレンズ・ホスピタル・オブ・フィラデルフィアThe Children’S Hospital Of Philadelphia 癌マーカーおよび治療標的としてのグリピカン２
CA3004913A1 (en) 2015-11-30 2017-06-08 Pioneer Hi-Bred International, Inc. Plant regulatory elements and methods of use thereof
DK3387134T3 (da) 2015-12-11 2020-12-21 Danisco Us Inc Fremgangsmåder og sammensætninger til øget nukleasemedieret genommodifikation og reducerede virkninger uden for målstedet
WO2017103822A1 (en) 2015-12-16 2017-06-22 The New Zealand Institute For Plant And Food Research Limited Compositions and methods for manipulating the development of plants
DK3390631T3 (da) 2015-12-18 2020-07-13 Danisco Us Inc Fremgangsmåder og sammensætninger til t-rna-baseret guide-rna-ekspression
WO2017106414A1 (en) 2015-12-18 2017-06-22 Danisco Us Inc. Methods and compositions for polymerase ii (pol-ii) based guide rna expression
US12110490B2 (en) 2015-12-18 2024-10-08 The Broad Institute, Inc. CRISPR enzymes and systems
WO2017106731A1 (en) 2015-12-18 2017-06-22 Monsanto Technology Llc Methods for producing corn plants with northern leaf blight resistance and compositions thereof
BR112018012887B1 (pt) 2015-12-22 2024-02-06 Pioneer Hi-Bred International, Inc Cassete de expressão, vetor, métodos de obtenção de célula vegetal e planta transgênica, métodos para expressar um polinucleotídeo
CN105638468B (zh) * 2016-01-06 2017-09-26 沈阳善达生物科技有限公司 高效诱导大豆子叶节外植体产生丛生芽的方法及培养基
WO2017136668A1 (en) 2016-02-04 2017-08-10 Yield10 Bioscience, Inc. Transgenic land plants comprising a putative bicarbonate transporter protein of an edible eukaryotic algae
US9896696B2 (en) 2016-02-15 2018-02-20 Benson Hill Biosystems, Inc. Compositions and methods for modifying genomes
WO2017144523A1 (en) 2016-02-22 2017-08-31 Evaxion Biotech Aps Proteins and nucleic acids useful in vaccines targeting staphylococcus aureus
CA3010628A1 (en) 2016-03-11 2017-09-14 Pioneer Hi-Bred International, Inc. Novel cas9 systems and methods of use
WO2017155715A1 (en) 2016-03-11 2017-09-14 Pioneer Hi-Bred International, Inc. Novel cas9 systems and methods of use
EP3426786A1 (en) 2016-03-11 2019-01-16 Altria Client Services LLC Compositions and methods for producing tobacco plants and products having reduced or eliminated suckers
EP3426780A1 (en) 2016-03-11 2019-01-16 Pioneer Hi-Bred International, Inc. Novel cas9 systems and methods of use
MX2018011534A (es) 2016-03-24 2019-07-04 Univ Leiden Metodos para transfectar plantas y para reducir eventos de integracion aleatoria.
EP3436049B1 (en) 2016-03-31 2022-01-12 Baylor Research Institute Angiopoietin-like protein 8 (angptl8)
CA3012362A1 (en) 2016-04-14 2017-10-19 Pioneer Hi-Bred International, Inc. Insecticidal polypeptides having improved activity spectrum and uses thereof
AR108284A1 (es) 2016-04-19 2018-08-08 Pioneer Hi Bred Int Combinaciones insecticidas de polipéptidos que tienen espectro de actividad mejorado y usos de éstas
AU2017253107B2 (en) 2016-04-19 2023-07-20 Massachusetts Institute Of Technology CPF1 complexes with reduced indel activity
EA201892293A1 (ru) 2016-05-04 2019-04-30 Пайонир Хай-Бред Интернэшнл, Инк. Инсектицидные белки и способы их применения
EP3463484B1 (en) 2016-05-27 2026-01-07 The Regents of the University of California Methods and compositions for targeting rna polymerases and non-coding rna biogenesis to specific loci
WO2017216704A1 (en) 2016-06-13 2017-12-21 Benson Hill Biosystems, Inc. Increasing plant growth and yield by using a phenylalanine ammonia lyase sequence
MX2018014993A (es) 2016-06-14 2019-09-06 Pioneer Hi Bred Int Uso de endonucleasa cpf1 para modificaciones de genoma de planta.
BR112018076047A2 (pt) 2016-06-16 2019-03-26 Pioneer Hi Bred Int elemento de silenciamento, construto de dna, cassete de expressão, célula hospedeira, composição, célula vegetal, planta ou parte de planta, semente transgênica, método para controlar um inseto-praga de planta e kit
US11788083B2 (en) 2016-06-17 2023-10-17 The Broad Institute, Inc. Type VI CRISPR orthologs and systems
WO2017216384A1 (en) 2016-06-17 2017-12-21 Evaxion Biotech Aps Vaccination targeting ichthyophthirius multifiliis
WO2017222773A1 (en) 2016-06-20 2017-12-28 Pioneer Hi-Bred International, Inc. Novel cas systems and methods of use
CA3028946A1 (en) 2016-06-22 2017-12-28 Benson Hill Biosystems, Inc. Increasing plant growth and yield by using an adp-glucose pyrophosphorylase sequence
WO2017221115A1 (en) 2016-06-23 2017-12-28 Benson Hill Biosystems, Inc. Increasing plant growth and yield by using a psan sequence
RU2019101793A (ru) 2016-06-24 2020-07-24 Пайонир Хай-Бред Интернэшнл, Инк. Регуляторные элементы растений и способы их применения
WO2017220787A1 (en) 2016-06-24 2017-12-28 Evaxion Biotech Aps Vaccines against aearomonas salmonicida infection
CA3029126A1 (en) 2016-06-29 2018-01-04 Benson Hill Biosystems, Inc. Increasing plant growth and yield by using a thioredoxin sequence
JP7099967B2 (ja) 2016-07-01 2022-07-12 リサーチ ディベロップメント ファウンデーション 幹細胞由来移植片からの増殖性細胞の排除
CA3026113A1 (en) 2016-07-01 2018-01-04 Pioneer Hi-Bred International, Inc. Insecticidal proteins from plants and methods for their use
WO2018013333A1 (en) 2016-07-12 2018-01-18 Pioneer Hi-Bred International, Inc. Compositions and methods to control insect pests
CN109688807B (zh) 2016-07-15 2023-04-28 巴斯夫欧洲公司 具有增加的除草剂耐受性的植物
EP3889167A1 (en) 2016-07-22 2021-10-06 Evaxion Biotech ApS Chimeric proteins for inducing immunity towards infection with s. aureus
EP3496528A4 (en) 2016-08-11 2020-03-18 Monsanto Technology LLC METHODS AND COMPOSITIONS FOR PRODUCING CORN PLANTS WITH RESISTANCE TO LATE FAILURE
WO2018035429A1 (en) 2016-08-18 2018-02-22 Wisconsin Alumni Research Foundation Peptides that inhibit syndecan-1 activation of vla-4 and igf-1r
JP2019530440A (ja) 2016-09-02 2019-10-24 ザ リージェンツ オブ ザ ユニバーシティ オブ カリフォルニア インターロイキン６受容体α結合単鎖可変断片を含む方法および組成物
US20190225974A1 (en) 2016-09-23 2019-07-25 BASF Agricultural Solutions Seed US LLC Targeted genome optimization in plants
WO2018064208A1 (en) 2016-09-28 2018-04-05 The Broad Institute, Inc. Systematic screening and mapping of regulatory elements in non-coding genomic regions, methods, compositions, and applications thereof
KR102598351B1 (ko) 2016-10-05 2023-11-08 후지필름 셀룰러 다이내믹스, 인코포레이티드 만능 줄기 세포를 hla 동형 접합 면역 세포로 직접 분화시키기 위한 방법
EP3523422B1 (en) 2016-10-05 2024-12-25 FUJIFILM Cellular Dynamics, Inc. Generating mature lineages from induced pluripotent stem cells with mecp2 disruption
EP3523439A1 (en) 2016-10-07 2019-08-14 Altria Client Services LLC Composition and methods for producing tobacco plants and products having reduced tobacco-specific nitrosamines (tsnas)
EP4342290A3 (en) 2016-10-07 2024-06-26 Altria Client Services LLC Tobacco plants having increased nitrogen efficiency and methods of using such plants
US11939586B2 (en) 2016-10-11 2024-03-26 Vib Vzw Means and methods for improved regeneration
EP3532620A1 (en) 2016-10-31 2019-09-04 Benson Hill Biosystems, Inc. Increasing plant growth and yield by using an erf transcription factor sequence
EP3535285B1 (en) 2016-11-01 2022-04-06 Pioneer Hi-Bred International, Inc. Insecticidal proteins and methods for their use
US12404514B2 (en) 2016-12-09 2025-09-02 The Broad Institute, Inc. CRISPR-systems for modifying a trait of interest in a plant
CN110381997A (zh) 2016-12-12 2019-10-25 茂体外尔公司 用于治疗和预防癌症和感染性疾病的包含病毒基因治疗和免疫检查点抑制剂的方法和组合物
CA3044404A1 (en) 2016-12-14 2018-06-21 Pioneer Hi-Bred International, Inc. Insecticidal proteins and methods for their use
EP3555299A1 (en) 2016-12-16 2019-10-23 Two Blades Foundation Late blight resistance genes and methods of use
EA201991300A1 (ru) 2016-12-20 2019-12-30 Басф Агро Б.В. Растения, обладающие повышенной толерантностью к гербицидам
US20190309317A1 (en) 2016-12-21 2019-10-10 Altria Client Services Llc Compositions and methods for producing tobacco plants and products having altered alkaloid levels
EP3558004A1 (en) 2016-12-22 2019-10-30 Pioneer Hi-Bred International, Inc. Insecticidal proteins and methods for their use
EP3342780A1 (en) 2016-12-30 2018-07-04 Dow AgroSciences LLC Pre-mrna processing factor 8 (prp8) nucleic acid molecules to control insect pests
EP3565576A1 (en) 2017-01-05 2019-11-13 Evaxion Biotech ApS Vaccines targeting pseudomonas aeruginosa
CA3047416A1 (en) 2017-01-20 2018-07-26 The Regents Of The University Of California Targeted gene activation in plants
WO2018140214A1 (en) 2017-01-24 2018-08-02 Pioneer Hi-Bred International, Inc. Nematicidal protein from pseudomonas
CA3047011A1 (en) 2017-01-26 2018-08-02 The Regents Of The University Of California Targeted gene demethylation in plants
WO2018148001A1 (en) 2017-02-08 2018-08-16 Pioneer Hi-Bred International Inc Insecticidal combinations of plant derived insecticidal proteins and methods for their use
AU2018224065B2 (en) 2017-02-22 2021-11-11 Nuseed Nutritional Us Inc. Transgenic land plants comprising enhanced levels of mitochondrial transporter protein
WO2018169787A1 (en) 2017-03-13 2018-09-20 Ball Horticultural Company Downy mildew resistant impatiens
EP3596207B1 (en) 2017-03-15 2023-12-20 The Broad Institute, Inc. Novel cas13b orthologues crispr enzymes and systems
WO2018175635A1 (en) 2017-03-21 2018-09-27 The Regents Of The University Of California Herbicidal compositions and methods of use thereof
EP3610009A1 (en) 2017-04-12 2020-02-19 The Broad Institute, Inc. Novel type vi crispr orthologs and systems
AU2018254442B2 (en) 2017-04-18 2024-03-28 FUJIFILM Cellular Dynamics, Inc. Antigen-specific immune effector cells
WO2018202800A1 (en) 2017-05-03 2018-11-08 Kws Saat Se Use of crispr-cas endonucleases for plant genome engineering
WO2018204777A2 (en) 2017-05-05 2018-11-08 The Broad Institute, Inc. Methods for identification and modification of lncrna associated with target genotypes and phenotypes
CN110621780B (zh) 2017-05-11 2024-03-19 先锋国际良种公司 杀昆虫蛋白及其使用方法
WO2018209209A1 (en) 2017-05-12 2018-11-15 Two Blades Foundation Methods for screening proteins for pattern recognition receptor function in plant protoplasts
US12297436B2 (en) 2017-05-18 2025-05-13 The Broad Institute, Inc. Systems, methods, and compositions for targeted nucleic acid editing
WO2018213679A1 (en) 2017-05-19 2018-11-22 Second Genome, Inc. Proteins for the treatment of epithelial barrier function disorders
US11535857B2 (en) 2017-05-22 2022-12-27 Benson Hill, Inc. Increasing plant growth and yield by using an ABC transporter sequence
WO2018217333A1 (en) 2017-05-26 2018-11-29 Pioneer Hi-Bred International, Inc. Insecticidal polypeptides having improved activity spectrum and uses thereof
WO2018220582A1 (en) 2017-05-31 2018-12-06 Tropic Biosciences UK Limited Methods of selecting cells comprising genome editing events
GB201708665D0 (en) 2017-05-31 2017-07-12 Tropic Biosciences Uk Ltd Compositions and methods for increasing extractability of solids from coffee beans
GB201708662D0 (en) 2017-05-31 2017-07-12 Tropic Biosciences Uk Ltd Compositions and methods for increasing shelf-life of banana
WO2019012483A1 (en) 2017-07-13 2019-01-17 Benson Hill Biosystems, Inc. INCREASING GROWTH AND YIELD OF PLANTS WITH FERREDOXINE-THIOREDOXIN REDUCTASE
US11261458B2 (en) 2017-07-28 2022-03-01 Two Blades Foundation Potyvirus resistance genes and methods of use
CN111373040B (zh) 2017-08-09 2024-07-12 本森希尔股份有限公司 修饰基因组的组合物和方法
WO2019035003A1 (en) 2017-08-17 2019-02-21 Benson Hill Biosystems, Inc. INCREASING GROWTH AND PLANT YIELD BY MEANS OF GLUTAREXIN
FI3675623T3 (fi) 2017-08-29 2025-08-15 Kws Saat Se & Co Kgaa Parannettuja sinisiä aleuroni- ja muita segregaatiojärjestelmiä
CN111212562B (zh) 2017-09-01 2023-06-27 奥驰亚客户服务有限公司 与烟草中提高的氮利用效率有关的方法和组合物
WO2019051232A1 (en) 2017-09-11 2019-03-14 Altria Client Services Llc COMPOSITIONS AND METHODS FOR PRODUCING TOBACCO PLANTS AND PRODUCTS HAVING REDUCED OR ELIMINATED GOURMETS
BR112020004764A2 (pt) 2017-09-11 2020-09-24 R.J. Reynolds Tobacco Company métodos e composições para aumentar a expressão de genes de interesse em uma planta por coexpressão com p21
SG11202002481RA (en) 2017-09-19 2020-04-29 Tropic Biosciences Uk Ltd Modifying the specificity of non-coding rna molecules for silencing gene expression in eukaryotic cells
AU2018338318B2 (en) 2017-09-21 2022-12-22 Massachusetts Institute Of Technology Systems, methods, and compositions for targeted nucleic acid editing
US20200270622A1 (en) 2017-09-25 2020-08-27 Pioneer Hi-Bred International, Inc. Tissue-preferred promoters and methods of use
US20200165626A1 (en) 2017-10-13 2020-05-28 Pioneer Hi-Bred International, Inc. Virus-induced gene silencing technology for insect control in maize
WO2019075295A1 (en) 2017-10-13 2019-04-18 Pioneer Hi-Bred International, Inc. SYSTEMS AND METHODS FOR CELL REPELLENCE OF A PLANT CELL
EP3704250A1 (en) 2017-11-03 2020-09-09 InteRNA Technologies B.V. Mirna molecule, equivalent, antagomir, or source thereof for treating and/or diagnosing a condition and/or a disease associated with neuronal deficiency or for neuronal (re)generation
US11634725B2 (en) 2017-11-03 2023-04-25 University Of Florida Research Foundation, Inc. Methods and compositions for plant pathogen resistance in plants
EP3480314A1 (en) 2017-11-03 2019-05-08 Philip Morris Products S.A. Regulation of alkaloid content
EP3710014A1 (en) 2017-11-14 2020-09-23 Henry Ford Health System Compositions for use in the treatment and prevention of cardiovascular disorders resulting from cerebrovascular injury
US20200291416A1 (en) 2017-11-21 2020-09-17 Benson Hill, Inc. Increasing plant growth and yield by using a quinone oxidoreductase
WO2019108619A1 (en) 2017-11-28 2019-06-06 Two Blades Foundation Methods and compositions for enhancing the disease resistance of plants
WO2019118463A1 (en) 2017-12-15 2019-06-20 Danisco Us Inc Cas9 variants and methods of use
EP3502259A1 (en) 2017-12-19 2019-06-26 Universiteit Leiden A combinational strategy for reducing random integration events when transfecting plants
EP3728294A4 (en) 2017-12-19 2021-12-29 Pioneer Hi-Bred International, Inc. Insecticidal polypeptides and uses thereof
WO2019123246A1 (en) 2017-12-19 2019-06-27 Benson Hill Biosystems, Inc. Modified agpase large subunit sequences and methods for detection of precise genome edits
US11034969B2 (en) 2017-12-22 2021-06-15 Altria Client Services Llc Plant comprising recombinant polynucleotides encoding a pigment regulatory transcription factor with a tissue-preferred promoter
WO2019126709A1 (en) 2017-12-22 2019-06-27 The Broad Institute, Inc. Cas12b systems, methods, and compositions for targeted dna base editing
WO2019136174A2 (en) 2018-01-03 2019-07-11 Uwm Research Foundation, Inc. Sterile mutant and two line breeding system
EP3735467A4 (en) 2018-01-05 2021-12-01 Ottawa Hospital Research Institute MODIFIED VACCINE VECTORS
CN111836539A (zh) 2018-01-12 2020-10-27 奥驰亚客户服务有限公司 用于产生烟草植物和具有经过改变的生物碱水平的产品的组合物和方法
EP3737226A1 (en) 2018-01-12 2020-11-18 Two Blades Foundation Stem rust resistance genes and methods of use
US11939587B2 (en) 2018-01-17 2024-03-26 Basf Se Plants having increased tolerance to herbicides
WO2019145399A1 (en) 2018-01-24 2019-08-01 Evaxion Biotech Aps Vaccines for prophylaxis of s. aureus infections
US11905518B2 (en) 2018-02-12 2024-02-20 Curators Of The University Of Missouri Small auxin upregulated (SAUR) gene for the improvement of root system architecture, waterlogging tolerance, drought resistance and yield in plants and methods of uses
EP3755792A4 (en) 2018-02-23 2021-12-08 Pioneer Hi-Bred International, Inc. NEW CAS9 ORTHOLOGIST
EP3759489A1 (en) 2018-03-02 2021-01-06 Pioneer Hi-Bred International, Inc. Plant health assay
BR112020018187A2 (pt) 2018-03-05 2021-04-27 Altria Client Services Llc composições e métodos para a produção de plantas e produtos de tabaco que possuem níveis alterados de alcaloides com qualidade de folha desejável
ES3036459T3 (en) 2018-03-12 2025-09-19 Pioneer Hi Bred Int Methods for plant transformation
EP3764796B1 (en) 2018-03-14 2025-12-03 Pioneer Hi-Bred International, Inc. Insecticidal proteins from plants and methods for their use
EP3764798B1 (en) 2018-03-14 2025-12-17 Pioneer Hi-Bred International, Inc. Insecticidal proteins from plants and methods for their use
KR20200135986A (ko) 2018-03-19 2020-12-04 멀티비르 인코포레이티드 종양 억제인자 유전자 치료 및 cd122/cd132 작용제를 포함하는 암 치료를 위한 방법 및 조성물
CN111902547B (zh) 2018-03-23 2024-06-14 先锋国际良种公司 鉴定、选择和产生疾病抗性作物的方法
CN111818795A (zh) 2018-03-28 2020-10-23 菲利普莫里斯生产公司 调节植物中的还原糖含量
CN111801423A (zh) 2018-03-28 2020-10-20 菲利普莫里斯生产公司 调节植物中的氨基酸含量
WO2019186274A2 (en) 2018-03-30 2019-10-03 University Of Geneva Micro rna expression constructs and uses thereof
EP3773017B1 (en) 2018-04-03 2022-06-08 Altria Client Services LLC Composition and methods for producing tobacco plants and products having increased phenylalanine and reduced tobacco-specific nitrosamines (tsnas)
US10968257B2 (en) 2018-04-03 2021-04-06 The Broad Institute, Inc. Target recognition motifs and uses thereof
CN111988988A (zh) 2018-04-18 2020-11-24 先锋国际良种公司 鉴定、选择和产生抗白叶枯病水稻的方法
GB201807192D0 (en) 2018-05-01 2018-06-13 Tropic Biosciences Uk Ltd Compositions and methods for reducing caffeine content in coffee beans
US11708580B2 (en) 2018-05-09 2023-07-25 Benson Hill, Inc. Increasing plant growth and yield by using a DUF2996 domain-containing protein
CA3096516A1 (en) 2018-05-22 2019-11-28 Pioneer Hi-Bred International, Inc. Plant regulatory elements and methods of use thereof
EP3800994A4 (en) 2018-05-24 2022-03-09 Monsanto Technology LLC GENOME EDITING IN PLANTS
US11851668B2 (en) 2018-06-06 2023-12-26 Huazhong Agricultural University Methods of identifying, selecting, and producing southern corn rust resistant crops
CA3102975A1 (en) 2018-06-07 2019-12-12 The State Of Israel, Ministry Of Agriculture & Rural Development, Agricultural Research Organization (Aro) (Volcani Center) Methods of regenerating and transforming cannabis
US11827892B2 (en) 2018-06-07 2023-11-28 The State Of Israel, Ministry Of Agriculture & Rural Development, Agricultural Research Organization (Aro) (Volcani Center) Nucleic acid constructs and methods of using same
AR115540A1 (es) 2018-06-14 2021-01-27 Benson Hill Biosystems Inc Aumento del crecimiento de las plantas y el rendimiento usando una proteína de la superfamilia de ring / u-box
BR112020026640A2 (pt) 2018-06-28 2021-04-06 Pioneer Hi-Bred International, Inc. Métodos para selecionar plantas transformadas
US20210324398A1 (en) 2018-06-29 2021-10-21 Pioneer Hi-Bred International, Inc. Edited nac genes in plants
CN112714792B (zh) 2018-08-02 2024-10-22 奥驰亚客户服务有限公司 经优化的组织优选性启动子和其用途
WO2020033601A1 (en) 2018-08-07 2020-02-13 The Broad Institute, Inc. Novel cas12b enzymes and systems
EP3844283A1 (en) 2018-08-29 2021-07-07 Pioneer Hi-Bred International, Inc. Insecticidal proteins and methods for their use
BR112021004034A2 (pt) 2018-09-04 2021-05-25 Yield10 Bioscience, Inc. plantas terrestres geneticamente modificadas que expressam uma proteína de rendimento de sementes aumentado e/ou um rna de rendimento de sementes aumentado
WO2020069313A2 (en) 2018-09-28 2020-04-02 Henry Ford Health System Use of extracellular vesicles in combination with tissue plasminogen activator and/or thrombectomy to treat stroke
EP3870207A1 (en) 2018-10-22 2021-09-01 Evaxion Biotech ApS Vaccines targeting m. catharrhalis
CN113166744B (zh) 2018-12-14 2025-02-07 先锋国际良种公司 用于基因组编辑的新颖crispr-cas系统
AU2019406778A1 (en) 2018-12-17 2021-07-22 Massachusetts Institute Of Technology Crispr-associated transposase systems and methods of use thereof
AU2020208190B2 (en) 2019-01-15 2022-07-28 Seminis Vegetable Seeds, Inc. Green bean plants with improved disease resistance
CN113660857A (zh) 2019-01-24 2021-11-16 奥驰亚客户服务有限公司 包含降低的烟碱和降低的烟草特异性亚硝胺的烟草植物
WO2020157164A1 (en) 2019-01-30 2020-08-06 Enobraq Modified plant with improved rubisco activity
WO2020171889A1 (en) 2019-02-19 2020-08-27 University Of Rochester Blocking lipid accumulation or inflammation in thyroid eye disease
EP3931206A1 (en) 2019-02-27 2022-01-05 Evaxion Biotech ApS Vaccines targeting h. influenzae
US12215382B2 (en) 2019-03-01 2025-02-04 The General Hospital Corporation Liver protective MARC variants and uses thereof
BR112021017998A2 (pt) 2019-03-11 2021-11-16 Pioneer Hi Bred Int Métodos para a produção de plantas clonais
GB201903520D0 (en) 2019-03-14 2019-05-01 Tropic Biosciences Uk Ltd Modifying the specificity of non-coding rna molecules for silencing genes in eukaryotic cells
GB201903519D0 (en) 2019-03-14 2019-05-01 Tropic Biosciences Uk Ltd Introducing silencing activity to dysfunctional rna molecules and modifying their specificity against a gene of interest
GB201903521D0 (en) 2019-03-14 2019-05-01 Tropic Biosciences Uk Ltd No title
WO2020191102A1 (en) 2019-03-18 2020-09-24 The Broad Institute, Inc. Type vii crispr proteins and systems
EP3947425A1 (en) 2019-03-27 2022-02-09 Pioneer Hi-Bred International, Inc. Plant explant transformation
WO2020198496A1 (en) 2019-03-28 2020-10-01 Pioneer Hi-Bred International, Inc. Modified agrobacterium strains and use thereof for plant transformation
JP2022526982A (ja) 2019-04-05 2022-05-27 ダニスコ・ユーエス・インク 線状組換えＤＮＡコンストラクトを使用してバチルス（Ｂａｃｉｌｌｕｓ）のゲノムにドナーＤＮＡ配列を組み込むための方法及びその組成物
CA3136113A1 (en) 2019-04-05 2020-10-08 Danisco Us Inc. Methods for polynucleotide integration into the genome of bacillus using dual circular recombinant dna constructs and compositions thereof
AU2020272047B2 (en) 2019-04-11 2026-03-12 The Regents Of The University Of California Engineered phosphoenolpyruvate carboxylase enzymes
US12543674B2 (en) 2019-04-18 2026-02-10 Pioneer Hi-Bred International, Inc. Embryogenesis factors for cellular reprogramming of a plant cell
AU2020273471B2 (en) 2019-05-14 2026-05-07 The University Of Chicago Methods and compositions comprising Staphylococcus protein A (SpA) variants
US12116583B2 (en) 2019-05-14 2024-10-15 Yield10 Bioscience, Inc. Modified plants comprising a polynucleotide comprising a non-cognate promoter operably linked to a coding sequence that encodes a transcription factor
US20220235340A1 (en) 2019-05-20 2022-07-28 The Broad Institute, Inc. Novel crispr-cas systems and uses thereof
WO2020236972A2 (en) 2019-05-20 2020-11-26 The Broad Institute, Inc. Non-class i multi-component nucleic acid targeting systems
BR112021026248A2 (pt) 2019-06-27 2022-03-03 Two Blades Found Molécula de ácido nucleico isolada que codifica uma proteína atrlp23 engenheirada, proteína atrlp23 engenheirada, cassete de expressão, vetor, célula hospedeira, planta ou célula vegetal, métodos para fazer uma proteína atrlp23 engenheirada, para fazer uma molécula de ácido nucleico que codifica uma proteína atrlp23 engenheirada e para intensificar a resistência de uma planta
WO2021011348A1 (en) 2019-07-12 2021-01-21 The Regents Of The University Of California Plants with enhanced resistance to bacterial pathogens
US20220257653A1 (en) 2019-07-19 2022-08-18 The Children's Hospital Of Philadelphia Chimeric antigen receptors containing glypican 2 binding domains
EP4017252A1 (en) 2019-08-23 2022-06-29 Pioneer Hi-Bred International, Inc. Methods of identifying, selecting, and producing anthracnose stalk rot resistant crops
WO2021041922A1 (en) 2019-08-30 2021-03-04 The Broad Institute, Inc. Crispr-associated mu transposase systems
CN114729381A (zh) 2019-09-05 2022-07-08 本森希尔股份有限公司 修饰基因组的组合物和方法
US11542513B2 (en) 2019-09-26 2023-01-03 Seminis Vegetable Seeds, Inc. Lettuce plants having resistance to Nasonovia ribisnigri biotype Nr:1
US12297426B2 (en) 2019-10-01 2025-05-13 The Broad Institute, Inc. DNA damage response signature guided rational design of CRISPR-based systems and therapies
WO2021072241A1 (en) 2019-10-10 2021-04-15 Altria Client Services Llc Compositions and methods for producing tobacco plants and products having altered alkaloid levels with desirable leaf quality via manipulating leaf quality genes
CN115175680A (zh) 2019-10-18 2022-10-11 加利福尼亚大学董事会 Plxdc激活剂及其用于治疗血管病症的用途
PY2070811A (es) 2019-11-01 2021-08-12 Purecircle Usa Inc Cultivar de stevia 18136109
EP3825408A1 (en) 2019-11-19 2021-05-26 FRAUNHOFER-GESELLSCHAFT zur Förderung der angewandten Forschung e.V. Methods of multi-species insect pest control
WO2021113337A1 (en) 2019-12-03 2021-06-10 Altria Client Services Llc Compositions and methods for producing tobacco plants and products having altered alkaloid levels
WO2021113644A1 (en) 2019-12-05 2021-06-10 Multivir Inc. Combinations comprising a cd8+ t cell enhancer, an immune checkpoint inhibitor and radiotherapy for targeted and abscopal effects for the treatment of cancer
JP7607903B2 (ja) * 2019-12-06 2025-01-06 株式会社アクトリー キヌアの目的遺伝子が導入された多芽体誘導法及び該誘導法で得られた多芽体
US20230050225A1 (en) 2020-01-06 2023-02-16 Evaxion Biotech A/S Vaccines targeting Neisseria gonorrhoeae
CN111153974A (zh) 2020-01-15 2020-05-15 华中农业大学 玉米抗病基因和分子标记及其应用
CN115516098A (zh) 2020-01-17 2022-12-23 奥驰亚客户服务有限公司 与改进的氮利用效率有关的方法和组合物
WO2021146641A1 (en) 2020-01-17 2021-07-22 The Broad Institute, Inc. Small type ii-d cas proteins and methods of use thereof
WO2021146508A2 (en) * 2020-01-17 2021-07-22 Asklepios Biopharmaceutical, Inc. Systems and methods for synthetic regulatory sequence design or production
WO2021173480A2 (en) 2020-02-24 2021-09-02 Pioneer Hi-Bred International, Inc. Intra-genomic homologous recombination
BR112022017197A2 (pt) 2020-02-28 2022-11-01 Pioneer Hi Bred Int Sistema de produção haploide duplicado de sorgo
US12467061B2 (en) 2020-04-15 2025-11-11 Pioneer Hi-Bred International, Inc. Plant pathogen effector and disease resistance gene identification, compositions, and methods of use
IL298558A (en) 2020-05-27 2023-01-01 Antion Biosciences Sa Adapter molecules redirect CAR T cells to the antigen of interest
BR112022024064A2 (pt) 2020-05-29 2023-01-31 Fujifilm Cellular Dynamics Inc Bicamada de epitélio pigmentado da retina e fotorreceptores e uso dos mesmos
PH12022552993A1 (en) 2020-05-29 2023-01-16 Fujifilm Cellular Dynamics Inc Retinal pigmented epithelium and photoreceptor dual cell aggregates and methods of use thereof
CN116171106A (zh) 2020-06-03 2023-05-26 奥驰亚客户服务有限公司 用于生产具有改变的生物碱水平的烟草植物和制品的组合物和方法
WO2021252238A1 (en) 2020-06-12 2021-12-16 Pioneer Hi-Bred International, Inc. Alteration of seed composition in plants
CA3186978A1 (en) 2020-07-14 2022-01-20 Pioneer Hi-Bred International, Inc. Insecticidal proteins and methods for their use
AU2021204717A1 (en) 2020-07-15 2022-02-03 Seminis Vegetable Seeds, Inc. Green Bean Plants with Improved Disease Resistance
EP4192848A1 (en) 2020-08-10 2023-06-14 Pioneer Hi-Bred International, Inc. Plant regulatory elements and methods of use thereof
MX2023001641A (es) 2020-08-10 2023-03-08 Du Pont Composiciones y metodos para mejorar la resistencia al tizon foliar norte?o en el maiz.
WO2022072335A2 (en) 2020-09-30 2022-04-07 Pioneer Hi-Bred International, Inc. Rapid transformation of monocot leaf explants
IL302088A (en) 2020-10-13 2023-06-01 Phytotech Labs Inc Methods and compositions for axillary shoot micropropagation of cannabis and related plants
CA3197681A1 (en) 2020-10-21 2022-04-28 Pioneer Hi-Bred International, Inc. Parthenogenesis factors and methods of using same
US20230407324A1 (en) 2020-10-21 2023-12-21 Pioneer Hi-Bred International, Inc. Doubled haploid inducer
US20240009241A1 (en) 2020-11-05 2024-01-11 Board Of Regents, The University Of Texas System Engineered t cell receptors targeting egfr antigens and methods of use
EP4244371A1 (en) 2020-11-12 2023-09-20 Technische Universität Darmstadt Production of cannabinoids/phytocannabinoids with a plant extract
US12173293B2 (en) 2020-11-20 2024-12-24 AgBiome, Inc. Compositions and methods for incorporation of DNA into the genome of an organism
CA3202849A1 (en) 2021-02-09 2022-08-18 University Of Houston System Oncolytic virus for systemic delivery and enhanced anti-tumor activities
US20240122865A1 (en) 2021-02-19 2024-04-18 Pfizer Inc. Methods of Protecting RNA
AU2022226265A1 (en) 2021-02-26 2023-09-07 Signalchem Plantech Corporation Methods of high production of polyphenols from red lettuces and uses thereof
GB202103256D0 (en) 2021-03-09 2021-04-21 Tropic Biosciences Uk Ltd Method for silencing genes
CN115216554A (zh) 2021-04-16 2022-10-21 华中农业大学 植物病原体效应子和疾病抗性基因鉴定、组合物和使用方法
WO2022226316A1 (en) 2021-04-22 2022-10-27 Precision Biosciences, Inc. Compositions and methods for generating male sterile plants
CA3216719A1 (en) 2021-05-03 2022-11-10 Astellas Institute For Regenerative Medicine Methods of generating mature corneal endothelial cells
TW202309268A (zh) 2021-05-07 2023-03-01 安斯泰來再生醫藥協會 產生成熟肝細胞的方法
EP4336997A1 (en) 2021-05-11 2024-03-20 Two Blades Foundation Methods for preparing a library of plant disease resistance genes for functional testing for disease resistance
WO2022251443A1 (en) 2021-05-26 2022-12-01 FUJIFILM Cellular Dynamics, Inc. Methods to prevent rapid silencing of genes in pluripotent stem cells
EP4351621A1 (en) 2021-06-09 2024-04-17 Morehouse School of Medicine Hevamine-related plant composition and methods
GB202109586D0 (en) 2021-07-02 2021-08-18 Tropic Biosciences Uk Ltd Method for editing banana genes
US20240287535A1 (en) 2021-07-02 2024-08-29 Tropic Biosciences UK Limited Delay or prevention of browning in banana fruit
US20240424079A1 (en) 2021-07-05 2024-12-26 Evaxion Biotech A/S Vaccines targeting Neisseria gonorrhoeae
AU2022324708A1 (en) 2021-08-06 2024-02-15 Kws Vegetables B.V. Durable downy mildew resistance in spinach
GB202112866D0 (en) 2021-09-09 2021-10-27 Tropic Biosciences Uk Ltd Resistance to fusarium wilt in a banana
GB202112865D0 (en) 2021-09-09 2021-10-27 Tropic Biosciences Uk Ltd Resistance to black sigatoka disease in banana
CA3233104A1 (en) 2021-09-21 2023-03-30 Benson Hill, Inc. Compositions and methods comprising plants with reduced lipoxygenase and/or desaturase activities
JP2024536851A (ja) 2021-09-27 2024-10-08 モンサント テクノロジー エルエルシー 単子葉植物種子から切除された胚外植片を形質転換するための組成物及び方法
WO2023056269A1 (en) 2021-09-30 2023-04-06 Two Blades Foundation Plant disease resistance genes against stem rust and methods of use
WO2023067574A1 (en) 2021-10-21 2023-04-27 Benson Hill, Inc. Compositions and methods comprising plants with modified sugar content
EP4430063A1 (en) 2021-11-09 2024-09-18 Benson Hill, Inc. Promoter elements for improved polynucleotide expression in plants
WO2023089556A1 (en) 2021-11-22 2023-05-25 Pfizer Inc. Reducing risk of antigen mimicry in immunogenic medicaments
US20230193310A1 (en) 2021-12-10 2023-06-22 Seminis Vegetabe Seeds, Inc. Lettuce plants having resistance to downy mildew
WO2023111961A1 (en) 2021-12-15 2023-06-22 Benson Hill, Inc. Spatio-temporal promoters for polynucleotide expression in plants
WO2023117661A1 (en) 2021-12-20 2023-06-29 Philip Morris Products S.A. Increasing anatabine in tobacco leaf by regulating methyl putrescine oxidase
WO2023117701A1 (en) 2021-12-21 2023-06-29 Philip Morris Products S.A. Modulation of nicotine production by alteration of nicotinamidase expression or function in plants
WO2023119135A1 (en) 2021-12-21 2023-06-29 Benson Hill, Inc. Compositions and methods for modifying genomes
GB2614309A (en) 2021-12-24 2023-07-05 Stratosvir Ltd Improved vaccinia virus vectors
US12004476B2 (en) 2022-01-26 2024-06-11 Monsanto Technology Llc. Cotton variety 20R750B3XF
WO2023144779A1 (en) 2022-01-28 2023-08-03 Pfizer Inc. Coronavirus antigen variants
US20230270073A1 (en) 2022-02-25 2023-08-31 Benson Hill, Inc. Compositions and methods comprising plants with modified anthocyanin content
US20230295661A1 (en) 2022-03-16 2023-09-21 University Of Houston System Persistent hsv gene delivery system
WO2023183918A1 (en) 2022-03-25 2023-09-28 Pioneer Hi-Bred International, Inc. Methods of parthenogenic haploid induction and haploid chromosome doubling
US20230340515A1 (en) 2022-04-01 2023-10-26 Benson Hill, Inc. Compositions and methods comprising plants with modified saponin content
US20260002170A1 (en) 2022-04-01 2026-01-01 Benson Hill, Inc. Compositions and methods comprising plants with modified organ size and/or protein composition
GB202206507D0 (en) 2022-05-04 2022-06-15 Antion Biosciences Sa Expression construct
US20250288657A1 (en) 2022-05-04 2025-09-18 Evaxion Biotech A/S Staphylococcal protein variants and truncates
JP2025519556A (ja) 2022-06-10 2025-06-26 リサーチ ディベロップメント ファウンデーション 操作されたＦｃＲＩＩｂ選択的ＩｇＧ１ Ｆｃバリアントおよびその使用
US20230404003A1 (en) 2022-06-21 2023-12-21 Seminis Vegetable Seeds, Inc. Novel qtls conferring resistance to cucumber mosaic virus
WO2024006911A1 (en) 2022-06-29 2024-01-04 FUJIFILM Holdings America Corporation Ipsc-derived astrocytes and methods of use thereof
WO2024023764A1 (en) 2022-07-27 2024-02-01 Benson Hill, Inc. Increasing gene expression for increased protein content in plants
US20260109992A1 (en) 2022-07-27 2026-04-23 Benson Hill, Inc. Decreasing gene expression for increased protein content in plants
WO2024023578A1 (en) 2022-07-28 2024-02-01 Institut Pasteur Hsc70-4 in host-induced and spray-induced gene silencing
AU2023337091A1 (en) 2022-09-09 2025-03-06 Eidgenössische Technische Hochschule Zürich Plant regulatory elements and uses thereof
EP4602060A1 (en) 2022-10-13 2025-08-20 Philip Morris Products S.A. Increasing leaf biomass and nitrogen use efficiency by regulating ntp2
EP4360451A1 (en) 2022-10-25 2024-05-01 Limagrain Europe Mutants for haploid induction
WO2024118881A1 (en) 2022-12-01 2024-06-06 Genencor International Bv Iterative muliplex genome engineering in microbial cells using a bidirectional selection marker system
EP4627076A1 (en) 2022-12-01 2025-10-08 Danisco Us Inc Iterative multiplex genome engineering in microbial cells using a recombinant self-excisable selection marker system
WO2024118882A1 (en) 2022-12-01 2024-06-06 Genencor International Bv Iterative multiplex genome engineering in microbial cells using a selection marker swapping system
EP4633362A1 (en) 2022-12-12 2025-10-22 BASF Agricultural Solutions US LLC Plants having increased tolerance to herbicides
WO2024127362A1 (en) 2022-12-15 2024-06-20 Benson Hill, Inc. Spatio-temporal promoters for polynucleotide expression in plants
WO2024130212A1 (en) 2022-12-16 2024-06-20 Turnstone Biologics Corp. Recombinant vaccinia virus encoding one or more natural killer cell and t lymphocyte inhibitors
WO2024158934A1 (en) 2023-01-24 2024-08-02 Yale University Compositions and methods for controlling t-dna copy number in transformed plants
WO2024166076A1 (en) 2023-02-10 2024-08-15 King Abdullah University Of Science And Technology Recombinant production of antimicrobial peptides in planta
WO2024186630A1 (en) 2023-03-03 2024-09-12 Henry Ford Health System Use of extracellular vesicles for the treatment of cancer
US20240301436A1 (en) 2023-03-10 2024-09-12 Benson Hill, Inc. Compositions and methods comprising plants with increased seed amino acid content
JP2026510998A (ja) 2023-03-20 2026-04-10 パイオニア ハイ－ブレッド インターナショナル， インコーポレイテッド 変更されたＰＡＭ認識を有するＣａｓポリペプチド
US12225874B2 (en) 2023-03-27 2025-02-18 Redsea Science And Technology Inc. Tomato plant designated ‘X22-31’
US20240327856A1 (en) 2023-03-28 2024-10-03 Benson Hill, Inc. Compositions and methods comprising plants with reduced raffinose family oligosaccharides and/or high protein content
WO2024201416A1 (en) 2023-03-31 2024-10-03 Benson Hill, Inc. Compositions and methods comprising plants with modified organ size and/or protein composition
GB202305021D0 (en) 2023-04-04 2023-05-17 Tropic Biosciences Uk Ltd Methods for generating breaks in a genome
MX2024004405A (es) 2023-04-14 2024-11-08 Seminis Vegetable Seeds Inc Métodos y composiciones de resistencia a peronospora en la espinaca
EP4455293A1 (en) 2023-04-25 2024-10-30 Limagrain Europe Methods for providing a plant having increased resistance to a virus of the luteoviridae family
GB202306619D0 (en) 2023-05-04 2023-06-21 Antion Biosciences Sa Cell
EP4728079A1 (en) 2023-06-13 2026-04-22 Confluence Genetics, LLC Plants with modified fatty acid and/or oil content
EP4743481A1 (en) 2023-07-14 2026-05-20 Two Blades Found METHODS FOR IMPROVING THE THERMOSTABILITY OF PLANT IMMUNE RECEPTORS
EP4743480A1 (en) 2023-07-15 2026-05-20 Two Blades Found Broad-spectrum polerover gene
US20250075226A1 (en) 2023-08-29 2025-03-06 University Of Freiburg Proteins for regulation of symbiotic infection and associated regulatory elements
WO2025050009A2 (en) 2023-09-01 2025-03-06 Children's Hospital Medical Center Identification of targets for immunotherapy in melanoma using splicing-derived neoantigens
WO2025059184A1 (en) 2023-09-12 2025-03-20 Pioneer Hi-Bred International, Inc. Methods and compositions for generating genome-edited paternal doubled haploids
WO2025059533A1 (en) 2023-09-13 2025-03-20 The Broad Institute, Inc. Crispr enzymes and systems
WO2025117544A1 (en) 2023-11-29 2025-06-05 The Broad Institute, Inc. Engineered omega guide molecule and iscb compositions, systems, and methods of use thereof
GB202402745D0 (en) 2024-02-27 2024-04-10 Antion Biosciences Sa Cell
WO2025184759A1 (en) 2024-03-04 2025-09-12 Pioneer Hi-Bred International, Inc. Maize plants comprising resistance to gray leaf spot and compositions and methods for selecting and producing the same
WO2026003150A1 (en) 2024-06-28 2026-01-02 Basf Se Plants with mutated tubulin polypeptide having increased tolerance to herbicides
US20260071285A1 (en) 2024-09-11 2026-03-12 Seminis Vegetable Seeds, Inc. Novel qtls conferring resistance to cucurbit aphid-borne yellow virus
WO2026062583A2 (en) 2024-09-20 2026-03-26 Confluence Genetics, Llc Compositions and methods for modifying genomes
GB202415862D0 (en) 2024-10-28 2024-12-11 Antion Biosciences Sa Intronic mirna

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4992375A (en) * 1983-11-25 1991-02-12 Monsanto Company Method of regenerating soybeans from cultured soybean cotyledonary nodes
WO1989012102A1 (en) * 1988-06-01 1989-12-14 The Texas A&M University System Method for transforming plants via the shoot apex
WO1992017598A1 (en) * 1991-03-29 1992-10-15 The Board Of Trustees Of The University Of Illinois Production fo transgenic soybean plants

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
Ｐｌａｎｔ Ｃｅｌｌ Ｒｅｐｏｒｔｓ ９（１９９１）Ｐ．６７１−６７５

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